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Effects of Corticosterone, cAMP, cGMP, Ca^(2+), and Protein Kinase C on Apoptosis of Mouse Thymocytes Induced by X-ray Irradiation

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Biomedical and Environmental Sciences 2008年第2期21卷 167-172页

作者： SHOU-LIAng GOng LI-HUA DOng guang-WEI LIU PIng-SHEng GOng WEN-TIAN LU HOng-guang ZHAO XIAO-JIng JIA YOng ZHAO Key Laboralory of Radiobiology Unit Ministry of Health （School of Public Health Jilin University）Changchun 130021 Jilin China Transplantation Biology Research Division State Key Laboratory of Membrane and Membrane Biotechnology Institute of Zoology Chinese Academy of SciencesBeijing 100080 China Key Laboratory of Molecular Enzymology and Engineering Ministry of Education Jinlin University Changchun 130023 Jilin China

Objective To observe the effects of signal factors of corticosterone （CS）, cAMP, cGMP, Ca^2＋ and protein kinase C （PKC） on lymphocyte apoptosis in mouse thymus induced by X-rays of 4 Gy in vitro. Methods The DNA lyric rate for thymocytes was measured by fluomspectrophotometry. Results The DNA lyric rate for thymocytes 4-8 hours after irradiation with 2-8 Gy was significantly higher than that in the control （P〈0.01）. As compared with the control, the DNA lyric rate for thymocytes treated with 0.01 μnol/L CS （P〈0.01）, 50 ng/mL cAMP （P〈0.01）, 0.05-0.4 μg/mL ionomycin （Iono, P〈0.05 or P〈0.01） or 0.05-0.4 ng/mL phorbol myristate acetate （PMA, P〈0.05 or P〈0.01）, respectively, was significantly increased, while the rate for thymocytes treated with 50 ng/mL cGMP was not significantly increased. The DNA lyric rate for thymocytes treated with 0.01 μmol/L CS （P〈0.01）, 50 ng/mL cAMP （P〈0.01）, 0.2 and 0.4 μg/mL Iono （P〈0.05）, and 0.2 and 0.4 ng/mL PMA （P〈0.05） plus 4-Gy irradiation, respectively, was significantly higher than that treated with single 4-Gy irradiation, while the rate for thymocytes treated with 50 ng/mL cGMP plus 4-Gy irradiation was not increased. When both 0.4 ***/mL Iono and 0.4 ng/mL PMA acted on the thymocytes, the DNA lyric rate for thymocytes was significantly higher than that in the control （P〈0.01）, the DNA lytic rate for thymocytes treated with both 0.4 μg/mL Iono and 0.4 ng/mL PMA plus 4-Gy irradiation was significantly higher than that treated with single 4-Gy irradiation （P〈0.05）, but was Iono plus 4-Gy irradiation or 0.4 ng/mL PMA plus 4-Gy irradiation. can promote thymocyte apoptosis induced by larger dose X-rays. not significantly higher than that treated with 0.4 μg/mL Conclusion CS, cAMP, Ca^2＋, and PKC signal factors can promote thymocyte apoptosis induced by larger dose X-rays.

关键词： Corticosterone cAMP cGMP Ca^2+ PKC X-ray irradiation Lymphocyte Apoptosis

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Development of Sequence Characterized Amplified Region (SCAR) Primers for the Detection of Resistance to Sporisorium reiliana in Maize

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Agricultural Sciences in China 2009年第8期8卷 910-919页

作者： SHI Hong-liang LI Xin-hai ZHAng De-gui XIE Chuan-xiao HAO Zhuan-fang LI Ming-shun PAN guang-tang ZHAng Sbi-huang Maize Research Institute Sichuang Agricultural University Ya ＇an 625014 P.R. China Institute of Crop Sciences Chinese Academy of Agricultural Sciences/National Key Facility of Crop Gene Resources and Genetic Improvement Beijing 100081 P.R. China

Head smut of maize （Zea mays L.）, which was caused by Sporisorium reiliana, occurred in most of the maize growing areas of the world. The purpose of this study was to develop SCAR markers for map-based cloning of resistance genes and MAS. Two sets of BC3 progenies, one （BC3Q） derived from the cross Qi319 （resistance）×Huangzao 4 （susceptible）, the other （BC3M） from Mol7 （resistance）× Huangzao 4 （susceptible）, were generated. Huangzao 4 was the recurrent parent in both progenies. A combination of BSA （bulked segregant analysis） with AFLP （amplified fragment length polymorphism） method was applied to map the genes involving the resistance to S. reiliana, and corresponding resistant and susceptible bulks and their parental lines were used for screening polymorphic AFLP primer pairs. One fragment of PI3M61-152 was converted into SCAR （sequence charactered amplified fragment） marker S130. The marker was mapped at chromosome bin 2.09, the interval of a major QTL region previously reported to contribute to S. reiliana resistance. Furthermore, S130 was highly and facilitate map-based cloni associated with resistance to S. reiliana, and could be useful for marker-assisted selection ng of resistance genes.

关键词： maize (Zea mays L.) Sporisorium reiliana bulked segregant analysis amplified fragment length polymorphism sequence characterized amplified region

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急性基底节梗死rs-fMRI度中心度的相关研究

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中国临床医学影像杂志 2021年第2期32卷 87-89页

作者：汤林峰王建良杨光昆山市中西医结合医院放射科江苏昆山215332 江苏大学附属昆山医院昆山市第一人民医院放射科江苏昆山215300

目的:运用静息态磁共振成像(rs-fMRI)技术,通过研究急性基底节梗死后大脑度中心度(Degree centrality,DC)的变化,探讨脑功能改变的可能形成机制。方法:收集急性基底节梗死患者以及正常对照组各32例,均进行rs-fMRI扫描,收集数据并采用DC... 详细信息

目的:运用静息态磁共振成像(rs-fMRI)技术,通过研究急性基底节梗死后大脑度中心度(Degree centrality,DC)的变化,探讨脑功能改变的可能形成机制。方法:收集急性基底节梗死患者以及正常对照组各32例,均进行rs-fMRI扫描,收集数据并采用DC的方法分析脑功能的情况,计算两组的DC值,观察两个组别有差异的脑区。结果:急性基底节梗死组左侧小脑后叶、左侧中央前回、左侧中央后回及左侧额上回、左侧额中回的DC值低于正常对照组(P<0.05),右侧边缘叶的DC值高于对照组(P<0.05)。结论:DC是一种描述脑网络的图论方法,反映了节点在脑网络信息传输中的作用,可从一个新的角度去研究急性基底节梗死后脑功能网络的改变。

关键词：脑梗死磁共振成像

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Clinical and oncologic findings of extraprostatic extension on needle biopsy in de novo metastatic prostate cancer

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Asian Journal of Andrology 2020年第4期22卷 427-431页

作者： Jun-Ru Chen Jin-Ge Zhao Sha Zhu Meng-Ni Zhang Ni Chen Jian-Dong Liu guang-Xi Sun Peng-Fei Shen Hao Zeng Department of Urology Institute of UrologyWest China HospitalSichuan UniversityChengdu 610041China Department of Pathology West China HospitalSichuan UniversityChengdu 610041China

This study aimed to explore the clinical and oncologic findings in patients with de novo metastatic prostate cancer(mPCa)and extraprostatic extension(EPE)on *** retrospectively evaluated data on 630 patients with de novo mPCa between January 2009 and December 2017 in the West China Hospital(Chengdu,China),including evaluating the relationships between EPE and other variables and the association of EPE with survival outcomes by the Chi-square test,Kaplan–Meier curves,and the Cox proportional-hazards *** was found in 70/630 patients,making a prevalence of 11.1%.The presence of EPE on biopsy was associated with higher Gleason scores and higher incidence of neuroendocrine differentiation(NED),intraductal carcinoma of the prostate(IDC-P),and perineural invasion(PNI).Compared with those without EPE,patients with EPE had shorter castration-resistant prostate cancer-free survival(CFS;median:14.1 vs 17.1 months,P=0.015)and overall survival(OS;median:43.7 vs 68.3 months,P=0.032).According to multivariate analysis,EPE was not an independent predictor for *** analyses demonstrated that patients with favorable characteristics,including negative NED or IDC-P status,Eastern Cooperative Oncology Group(ECOG)scoreng ml−1,had worse prognoses if EPE was *** patients with PSAng ml−1,EPE was a negative independent predictor for OS(hazard ratio[HR]:4.239,95%confidence interval[CI]:1.218–14.756,P=0.023).EPE was strongly associated with other aggressive clinicopathological features and poorer CFS and *** data suggest that EPE may be an indicator of poor prognosis,particularly in patients,otherwise considered likely to have favorable survival outcomes.

关键词： extraprostatic extension metastatic prostate cancer prevalence prognosis

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The prognostic factors of effective ketoconazole treatment for metastatic castration-resistant prostate cancer： who can benefit from ketoconazole therapy？

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Asian Journal of Andrology 2012年第5期14卷 732-737页

作者： Guo-Wen Lin Xu-Dong Yao Ding-Wei Ye Yao Zhu Shi-Lin Zhang Bo Dai Hai-Liang Zhang Yi-Jun Shen Chun-guang Ma Department of Urology Fudan University Shanghai Cancer CenterShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China

We investigated the prognostic value of some variables of effective ketoconazole treatment for metastatic castration-resistant prostate cancer （mCRPC）. In total, 163 patients with mCRPC were eligible, receiving ketoconazole 200-400 mg three times daily with replacement doses of prednisone. Progression-free survival （PFS） was calculated from the beginning of the ketoconazole therapy to the onset of disease progression. The prognostic value of different variables for PFS was assessed by Cox regression analysis. The median PFS was 2.6 months （0.5-8.6 months） for these patients. The serum testosterone level changed during therapy, which decreased when the prostate-specific antigen （PSA） declined; the serum testosterone level increased as the levels of PSA relapsed. The median PFS values for patients associated with different factors were the following： 1.4 and 3.5 months for a nadir PSA of ≥ 0.2 and 〈0.2 ng ml- 1, respectively （hazard rate （HR）=4.767, P〈0.001）; 3.1 and 1.6 months for a baseline testosterone of ≥0.1 and 〈0.1 ng m1-1, respectively （HR=2.865, P=0.012）; 2.8 and 1.9 months for a baseline haemoglobin of ≥ 120 and 〈120 g 1-1, respectively （HR= 1.605, P〈0.001）; and 3.0 and 1.9 months for a PSA doubling time （PSADT） of ≥ 2.0 and 〈2.0 months, respectively （HR= 1.454, P=-0.017）. A risk model was constructed according to the four factors that divided patients into three subgroups of low risk （0-1 factors）, moderate risk （2 factors） and high risk （3-4 factors） with PFS values of 3.6, 3.0 and 1.4 months, respectively （HR=1.619, P〈0.001）. A nadir PSA of ≥0.2 ng m1-1, a baseline testosterone of 〈0.1 ng m1-1, a baseline haemoglobin of 〈 120 g I- 1 and a PSADT of 〈2 months were associated with a poor PFS. This risk model could provide evidence to predict the survival benefit of ketoconazole therapy.

关键词： castration-resistant prostate cancer ketoconazole therapy predictor progression-free survival

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贵州省喀斯特地区土地可持续利用评价研究

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国土资源科技管理 2009年第3期26卷 61-63页

作者：姜英聂光华贵州交通职业技术学院贵州贵阳550008 贵州大学贵州贵阳550003

以联合国粮农组织提出的土地可持续评价指标为依据,从生产的稳定性、资源的安全性、生态的保持性、经济的可行性和社会的可接受性等五个方面建立了指标体系,对贵州喀斯特地区的土地利用进行可持续的评价研究,评价结果表明贵州喀斯特地... 详细信息

以联合国粮农组织提出的土地可持续评价指标为依据,从生产的稳定性、资源的安全性、生态的保持性、经济的可行性和社会的可接受性等五个方面建立了指标体系,对贵州喀斯特地区的土地利用进行可持续的评价研究,评价结果表明贵州喀斯特地区的土地可持续利用度得到了不断的提高,但是总体水平较低,特别是在资源安全性和经济可行性方面有待进一步加强。

关键词：可持续利用评价喀斯特贵州省

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状态依存的公司治理模式研究

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西安财经学院学报 2016年第1期29卷 37-42页

作者：张完定王广三吕骊歌西安财经学院商学院陕西西安710100

不同公司治理模式的本质区别在于谁应该从公司的决策和行动中获益。文章提出风险—贡献模型,用两个维度,承担的风险和对企业价值的贡献将空间分为4个象限,并根据利益相关者在二维空间中的位置来决定在公司治理中的地位。这说明治理模式... 详细信息

不同公司治理模式的本质区别在于谁应该从公司的决策和行动中获益。文章提出风险—贡献模型,用两个维度,承担的风险和对企业价值的贡献将空间分为4个象限,并根据利益相关者在二维空间中的位置来决定在公司治理中的地位。这说明治理模式内生于这两个变量。利用这一模型,文章分析了美国的股东模式和日本的利益相关者模式企业公司治理的特点,分析表明,不同的治理模式只是模型的特定情况,因而可以将不同治理模式统一在同一分析框架下。最后,还根据中国公司的特点对中国公司治理模式提出了建议。

关键词：公司治理模式股东利益相关者风险一贡献模型

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纽约市族群地位的历史考察——对《1992年纽约市族群地位的调查》的一种解读

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华东师范大学学报（哲学社会科学版） 2005年第5期37卷 89-95页

作者：林广华东师范大学中国现代城市研究中心上海200062

纽约是个典型的移民城市,每个移民群体都为纽约的发展作出过或大或小的贡献。《1992年纽约市族群地位的调查》证实,犹太裔对纽约城市发展影响最大,西班牙裔影响最小;而经济地位、参政意识和公众影响力则对族群地位的高低起着决定性的作用。

关键词：纽约移民族群地住

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Evaluation of PSA-age volume score in predicting prostate cancer in Chinese population

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Asian Journal of Andrology 2018年第4期20卷 324-329页

作者： Yi-Shuo Wu Xiao-Bo Wu Ning Zhang guang-Liang Jiang Yang Yu Shi-Jun Tong Hao-Wen Jiang Shan-Hua Mao Rong Na Qiang Ding Department of Urology Huashan Hospital Fudan University Shanghai 200040 China Urology Research Center Fudan University Shanghai 200040 China Department of Urology Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China

This study was performed to evaluate prostate-specific antigen-age volume （PSA-AV） scores in predicting prostate cancer （PCa） in a Chinese biopsy population. A total of 2355 men who underwent initial prostate biopsy from January 2006 to November 2015 in Huashan Hospital were recruited in the current study. The PSA-AV scores were calculated and assessed together with PSA and PSA density （PSAD） retrospectively. Among 2133 patients included in the analysis, 947 （44.4%） were diagnosed with PCa. The mean age, PSA, and positive rates of digital rectal examination result and transrectal ultrasound result were statistically higher in men diagnosed with PCa （all P 〈 0.05）. The values of area under the receiver operating characteristic curves （AUCs） of PSAD and PSA-AV were 0.864 and 0.851, respectively, in predicting PCa in the entire population, both performed better than PSA （AUC = 0.805; P 〈 0.05）. The superiority of PSAD and PSA-AV was more obvious in subgroup with PSA ranging from 2.0 ng ml^-1 to 20.0 ng ml^-1. A PSA-AV score of 400 had a sensitivity and specificity of 93.7% and 40.0%, respectively. In conclusion, the PSA-AV score performed equally with PSAD and was better than PSA in predicting PCa. This indicated that PSA-AV score could be a useful tool for predicting PCa in Chinese population.

关键词： age China prostate cancer prostate-specific antigen volume

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Analysis of 19-nortestosterone residue in animal tissues by ion-trap gas chromatography-tandem mass spectrometry

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第6期12卷 460-467页

作者： Jin-qing JIAng Lei ZHAng guang-ling LI Hai-tang ZHAng Xue-feng YAng Jun-wei LIU Ren-feng LI Zi-liang WAng Jian-hua WAng College of Veterinary Medicine Northwest A &F University Yangling 712100 China College of Animal Science Henan Institute of Science and Technology Xinxiang 453003 China College of Resources and Environment Henan Institute of Science and Technology Xinxiang 453003 China

A rapid sample treatment procedure for the gas chromatography-tandem mass spectrometry （GC-MS） determination of 19-nortestosterone （19-NT） in animal tissues has been developed. In our optimized procedures, enzymatic hydrolysis with β-glucuronidase from Escherichia coliwas performed in an acetate buffer （pH 5.2, 0.2 mol/L） Next, the homogenate was mixed with methanol and heated at 60℃ for 15 min, then placed in an ice-bath at -18℃ for 2 h. After liquid-liquid extraction with n-hexane, the analytes were subjected to a normal-phase solid phase ex- traction （SPE） C18 cartridge for clean-up. The dded organic extracts were derivatized with heptafluorobutydc anhydride （HFBA）, and then the products were injected into GC-MS. Using electron impact mass spectrometry （El-MS） with positive chemical ionization （PCI）, four diagnostic ions （mlz 666, 453, 318, and 306） were determined. A standard calibration curve over the concentration range of 1-20 ng/g was reached, with Y=467084X-68354 （R^2=0.9997） for 19-NT, and the detection limit was 0.3 ng. When applied to spiked samples collected from bovine and ovine, the recoveries ranged from 63% to 101% with relative standard deviation （RSD） between 2.7% and 8.9%. The procedure is a highly efficient, sensitive, and more economical method which offers considerable potential to resolve cases of suspected nandrolone doping in husbandry animals.

关键词： 19-Nortestosterone (19-NT) Gas chromatography-tandem mass spectrometry (GC-MS) Animal tissues

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