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Preoperative serum liver enzyme markers for predicting early recurrence after curative resection of hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2015年第2期14卷 178-185页

作者： Zhong-Xia Wang Chun-Ping Jiang Yin Cao guang Zhang Wei-Bo Chen Yi-Tao Ding Department of Hepatobiliary Surgery the Affiliated Drum Tower Hospital of Nanjing University Medical School Department of Hepatobili-ary Surgery Nanjing Drum Tower Hospital Clinical Medical College of Nanjing Medical University Department of Hepatobiliary Surgery Nanjing Drum Tower Hospital Clinical Medical College of Nanjing Medical University

BACKGROUND：Early recurrence of hepatocellular carcinoma（HCC）is associated with worse prognosis after liver resection This study aimed to investigate the prognostic value of com mon liver enzyme markers in HCC early recurrence after cu rative hepatectomy and to establish a simple predictive model for HCC early ***： A total of 200 patients who had undergone curative resection for HCC were retrospectively analyzed. The patients were divided into early recurrence （within 2 years） and non-early recurrence groups. Demographical characteristics, preopera- tive liver function parameters, surgical factors and tumor related factors of the patients were assessed by univariate analysis to identify potential significant predictors for early recurrence after resection of HCC. Parameters with statisti- cal significance were entered into a Cox proportional hazard model to find independent risk factors. Receiver operating characteristic analysis was done to determine optimal cut-off values and the number of combined factors in multi-factor predictive model. RESULTS： Of 13 potential risk factors for early recurrence identified by univariate analysis, high lactate dehydrogenase （LDH〉206 U/L, HR=1.711, P=0.006）, high aspartate aminotransferase （AST）/alanine aminotransferase （ALT） ratio （AST/ ALT〉0.96, HR=1.769, P=0.006）, elevated alpha-fetoprotein （AFP〉8.6 ng/mL, HR=2.079, P=0.007）, small resection margin （〈1 cm, HR=2.354, P〈0.001） and advanced TNM stage （TNM III-IV, HR=2.164, P〈0.001） were independent risk factors for early recurrence of HCC shown by multivariate analysis. Patients with three or more concurrent independent risk factors had significantly higher risk for early recurrence than those with low risk factors. The sensitivity and specificity of this predictive model are 53.6% and 80.7%, respectively （area under curve=0.741, 95% CI 0.674-0.800, P〈0.0001）. CONCLUSIONS： Preoperative common fiver enzyme markers, LDH and AST/ALT ratio, were independently associated

关键词： hepatocellular carcinoma liver enzyme recurrence resection hepatectomy

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肥胖与神经调节

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生理科学进展 2001年第1期32卷 45-51页

作者：黄之瑜孙志娟范少光北京大学医学部生理学系北京100083

机体的能量获取和能量消耗 ,在一定时期内 ,是处于一种相对平衡的状态 :获取的能量等于消耗的能量。在这一调节中 ,神经系统起有重要的作用。如果获取的能量 (进食 )大于消耗的能量 ,将产生肥胖。由于很多疾病与肥胖的产生有密切的关系 ... 详细信息

机体的能量获取和能量消耗 ,在一定时期内 ,是处于一种相对平衡的状态 :获取的能量等于消耗的能量。在这一调节中 ,神经系统起有重要的作用。如果获取的能量 (进食 )大于消耗的能量 ,将产生肥胖。由于很多疾病与肥胖的产生有密切的关系 ,因此 ,对能量平衡调节的研究越来越受到重视。本文简要总结了近年来这方面的研究进展。内容包括 :(1)饱感的产生与进食的终止 ;(2 )机体脂肪储存信号与进食的调节 ;(3)与进食有关的中枢 ;(4)下丘脑中传递与进食有关信息的一级和二级神经元 ;(5)

关键词：肥胖能量平衡神经调节发病机理

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Distribution characteristics of poly-and perfluoroalkyl substances in the Yangtze River Delta

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Journal of Environmental Sciences 2017年第11期29卷 97-109页

作者： Binghui Zheng Xiaolei Liu Rui Guo Qing Fu Xingru Zhao Shanjun Wang Sheng Chang Xing Wang Mengjiao Geng guang Yang State Environmental Protection Key Laboratory of Source Water Protection Chinese Research Academy of Environmental Sciences College of Chemistry and Materials Science Hebei Normal University

In this work, a method was developed and optimized for the analysis of polyfluoroalkyl and/or perfluoroalkyl substances（PFASs） content in surface water and sediment samples with high instrumental response and good separation. Surface water and sediment samples were collected from the Yangtze River Delta（YRD） to analyze the distribution characteristics of perfluoroalkyl carboxylic acids（PFCAs）, perfluoroalkane sulfonic acids（PFSAs）, perfluoroalkyl phosphonic acids（PFPAs）, perfluoroalkyl phosphinic acids（PFPiAs）, and polyfluoroalkyl phosphoric acid diesters（di PAPs）. The results showed that the total concentrations of PFCAs and PFSAs in YRD varied from 31 to 902 ng/L. PFCAs（≥ 11 carbons） and PFSAs（≥ 10 carbons atoms） were not detected in any surface water samples. The mean concentrations of all PFCAs and PFSAs in surface water from the sampling areas decreased in the following order：Yangtze river（191 ng/L） ≈ Taihu lake（189 ng/L）〉 Huangpu river（122 ng/L） ≈ Qiantang river（120 ng/L）〉 Jiaxing urban river（100 ng/L）. Strong significant（p 〈 0.05） correlations between the concentrations of many of the compounds were found in the sampling areas, suggesting a common source for these compounds. Only perfluorooctanoic acid（PFOA） was observed in all sediment samples, at concentrations varying from 0.02 to 1.35 ng/g. Finally, detection rates of two di PAPs were only 8% and 10%, respectively and the concentration of di PAPs was two to three times lower compared to PFCAs and PFSAs.

关键词： Perfluorinated compounds Polyfluoroalkyl substances Surface water Sediment

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A New Method for Ultra-sensitive P24 Antigen Assay Based on Near-infrared Fluorescent Microsphere Immunochromatography

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Biomedical and Environmental Sciences 2020年第3期33卷 174-182页

作者： WAng Qi HOU Mei Ling LIU Li Peng MA Jing ZHAng Xiao guang ZHOU Zhi Xiang CAO Yu Xi Beijing University of Technology College of Life Science and BioengineeringBeijing 100124China Beijing Runbio Biotechnology Development Co. LtdBeijing 100106China National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

Objective To develop a rapid,highly sensitive quantitative method for detecting P24 antigen based on near-infrared fluorescent microsphere *** First,we prepared a lateral flow assay test strip,and labeled the detection antibody using a fluorescent ***,we optimized the antibody labeling ***,we optimized the detection ***,we created a working ***,we conducted a methodological assessment of the established fluorescent microsphere immunochromatography ***-six clinical samples were tested,and we compared the established fluorescent microsphere immunochromatography with the quantitative ELISA *** According to the working curve,the detection limit of the method is 3.4 pg/mL,and the detection range is 3.4 pg/mL to 10 ng/*** average intra-assay recovery was 99.6%,and the Coefficient of Variation(CV)was 5.4%–8.6%;the average inter-assay recovery was 97.3%,and the CV was 8.5%–11%.The detection rate of fluorescent microsphere immunochromatography was higher than ELISA method,and had a good correlation with *** The P24 antigen quantitative detection method based on near-infrared fluorescent microsphere immunochromatography has the advantages of rapid detection,high sensitivity,and wide detection range;thus,it is suitable for early clinical diagnosis and continuous monitoring of AIDS.

关键词： Fluorescent microsphere immunochromatography HIV P24 antigen POCT

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乙烯裂解炉管HP40合金的蠕变性能

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天津大学学报 2011年第10期44卷 930-935页

作者：安俊超荆洪阳徐连勇胡庆广天津大学材料科学与工程学院天津市现代连接技术重点实验室天津300072

在900,℃不同应力条件下,对HP40合金高温下的蠕变性能进行研究,观察试验前后显微组织的转变,并得到其蠕变本构方程.同时借助SEM+EDX对组织转变及相成分变化进行深入分析.HP40合金在高温下,随着应力的增加蠕变速率增大.其原始组织骨架状... 详细信息

在900,℃不同应力条件下,对HP40合金高温下的蠕变性能进行研究,观察试验前后显微组织的转变,并得到其蠕变本构方程.同时借助SEM+EDX对组织转变及相成分变化进行深入分析.HP40合金在高温下,随着应力的增加蠕变速率增大.其原始组织骨架状的共晶碳化物(r+M7C3+少量M23C6),经过长时蠕变试验后,逐渐变成条状和块状的M23C6,且有一定量的二次碳化物析出.HP40合金中骨架状共晶碳化物的消失会造成蠕变强度的下降.同时由于蠕变孔洞的出现,导致蠕变性能的下降,加速其蠕变变形的过程.

关键词：乙烯裂解炉管 HP40合金蠕变

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Determination of levels of adenosine phosphates in blood by ion chromatography

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Chinese Chemical Letters 2011年第12期22卷 1485-1488页

作者： Pei Jin Xie Ming Li Ye Zhong Yang Hu guang Wen Pan Yan Zhu Jia Jie Zhang Department of Chemistry Zhejiang UniversityHangzhou 310028China Shanghai Lab Dionex China Co.Ltd.Shanghai 201203China

A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange *** three adenosine phosphates were separated by Dionex IonPac AG18（50 mm×4 mm） guard column and IonPac AS 18（250 mm×4 mm） analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits（S/N=3） of AMP,ADP and ATP were 38,47,108 ng/L,*** relative standard deviations of retention time,peak area and peak height were all less than 1.87%and a good linear relationship was *** method was applied to analyze human blood samples.

关键词： Adenosine phosphate Ion chromatography Blood

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Protective Effect of Pyrroloquinoline Quinone on TNF-α-induced Mitochondrial Injury in Chondrocytes

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Current Medical Science 2021年第1期41卷 100-107页

作者： guang-tao HAN Wei-song CAI Yu-biao ZHAng Si-qi ZHOU Bin HE Hao-huan LI Department of Orthopedics Renmin Hospital of Wuhan UniversityWuhan430060China

Osteoarthritis(OA)is a degenerative disease characterized by matrix degradation and cell death leading to a gradual loss of articular cartilage *** a bacterial synthesis of quinine,pyrroloquinoline quinone(PQQ)is a strong redox cofactor with a variety of biological benefits,including antioxidant,anti-inflammation-induced mitochondrial metabolism *** study was designed to investigate the effect of PQQ on TNF-α-induced mitochondrial damage in *** isolated from C57BL/6 mice were exposed to TNF-α50 ng/mL,TNF-α50 ng/mL+PQQ 10µmol/L for 24 ***,morphological study,functional study and mechanism study were *** results revealed TNF-α-induced chondrocyte mitochondrion damage could be reduced by application of PQQ,evidenced by elevated number of mitochondria,well-kept mtDNA integrity,preserved ATP level,reestablished mitochondrial membrane potential,and prevented mitochondrial *** present work strongly suggests that the mitochondrion is an important target for OA chondrocyte damage induced by TNF-αand the PQQ protection from this damage ameliorates mitochondrial dysfunction induced by TNF-α.PQQ might be a potential chemical for OA intervention.

关键词： pyrroloquinoline quinone mitochondrial injury osteoarthritis TNF-α chondrocyte

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Alterations in gut microbiota during remission and recurrence of diabetes after duodenal-jejunal bypass in rats

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World Journal of Gastroenterology 2016年第29期22卷 6706-6715页

作者： Ming-Wei Zhong Shao-Zhuang Liu guang-Yong Zhang Xiang Zhang Teng Liu San-Yuan Hu Department of General Surgery Qilu Hospital of Shandong University

AIM: To observe the alterations in gut microbiota in high-fat diet(HFD)-induced diabetes recurrence after duodenal-jejunal bypass(DJB) in rats. METHODS: We assigned HDF- and low-dose streptozotocin-induced diabetic rats into two major groups to receive DJB and sham operation respectively. When the DJB was completed, we used HFD to induce diabetes recurrence. Then, we grouped the DJB-operated rats by blood glucose level into the DJB-remission(DJB-RM) group and the DJB-recurrence(DJB-RC) group. At a sequence of time points after operations, we compared calorie content in the food intake(calorie intake), oral glucose tolerance test, homeostasis model assessment of insulin resistance(HOMA-IR), concentrations of glucagon-like peptide 1(GLP-1), serum insulin, total bile acids(TBAs) and lipopolysaccharide(LPS) and alterations in colonic ***: The relative abundance of Firmicutes in the control(58.06% ± 11.12%; P ng/m L; P ng/m L) and decreased LPS(0.12 ± 0.04 ng/m L, P ng/m L) were observed the in DJB-RM group; however, these improvements were reversed in the DJB-RC group, with the exception of GLP-1(DJB-RM vs DJB-RC P > 0.05). CONCLUSION: Alterations in gut

关键词： Duodenal-jejunal bypass Gut microbiota Diabetes recurrence Lipopolysaccharide Total bile acids

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Characteristics of elevated cardiac troponin I in patients with acute ischemic stroke

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Journal of Geriatric Cardiology 2017年第6期14卷 401-406页

作者： Yu-Xia CUI Hui REN Chong-You LEE Su-Fang LI Jun-Xian SOng Xu-guang GAO Hong CHEN Department of Cardiology Peking University People's Hospital Beijing China Beijing Key Laboratory of Early Prediction and Intervention of Acute Myocardial Infarction Peking University People's Hospital Beijing China Center for Cardiovascular Translational Research Peking University People's Hospital Beijing China Department of Neurology Peking University People's Hospital Beijing China

Objective To study prognostic characteristics of cardiac troponin I （cTnI） elevation in acute ischemic stroke. Methods We retrospectively studied patients （n = 248） with acute ischemic stroke, acute ST-segment elevation myocardial infarction, and acute non-ST-elevation myocardial infarction who were treated between January 2013 and October 2015. Baseline demographic data and changes in cTnI levels among these three groups were compared. Patients with acute ischemic stroke were assigned to either the cTnI elevation group （cTnI 〉 0.034 ng/mL） or the no cTnI elevation group （cTnI ≤ 0.034 ng/mL）. Logistic regression analysis was used to identify risk factors associated with elevated serum cTnI in patients with acute ischemic stroke. Moreover, the duration of hospital stay and incidence of major cardiovascular outcomes were compared in patients with acute ischemic stroke, with or without elevated cTnI. Results In this study population of patients with acute ischemic stroke （n = 178）, acute ST-segment elevation myocardial infarction （n = 35）, and acute non-ST-elevation myocardial infarction （n = 35）, patients with acute ischemic stroke with elevated cTnI comprised 18.54% of subjects. Patients with elevated cTnI were older and more likely to have a history of hypertension. In addition, these patients had higher levels of inflammatory markers, reduced renal functions, increased D-dimer levels, higher NIH stroke scores, and lower left ventricular ejection fractions. Logistic regression analysis showed that both percentage of neutrophil and NIH stroke scores were elevated; estimated glomerular filtration rate and left ventricular ejection fraction were decreased in patients with acute ischemic stroke who had elevated cTnI, and they had more frequent major cardiovascular events during hospital stay. Conclusion Elevated cTnI detected in patients with acute ischemic stroke, indicated a greater likelihood of poor short-term prognosis during hospital stay.

关键词： Acute ischemic stroke Acute myocardial infarction Cardiac troponin I

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A New Method for Blood NT-proBNP Determination Based on a Near-infrared Point of Care Testing Device with High Sensitivity and Wide Scope

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Biomedical and Environmental Sciences 2017年第6期30卷 426-431页

作者： ZHAng Xiao guang SHU Yao Gen GAO Ju WAng Xuan LIU Li Peng WAng Meng CAO Yu Xi ZEng Yi National lnstitute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Beijing Runbio Biotechnology Development Co. Ltd Beijing 100106 China No.2 People's Hospital of Dali City Dali 671003 Yunnan China Department of Laboratory Medicine Beijing Hospital National Center of Gerontology Beijing 100730 China

Objective To develop a rapid, highly sensitive, and quantitative method for the detection of NT-proBNP levels based on a near-infrared point-of-care diagnostic （POCT） device with wide scope. Methods The lateral flow assay （LFA） strip of NT-proBNP was first prepared to achieve rapid detection. Then, the antibody pairs for NT-proBNP were screened and labeled with the near-infrared fluorescent dye Dylight-800. The capture antibody was fixed on a nitrocellulose membrane by a scribing device. Serial dilutions of serum samples were prepared using NT-proBNP-free serum series. The prepared test strips, combined with a near-infrared POCT device, were validated by known concentrations of clinical samples. The POCT device gave the output of the ratio of the intensity of the fluorescence signal of the detection line to that of the quality control line. The relationship between the ratio value and the concentration of the specimen was plotted as a work curve. The results of 62 clinical specimens obtained from our method were compared in parallel with those obtained from the Roche E411 kit. Results Based on the log-log plot, the new method demonstrated that there was a good linear relationship between the ratio value and NT-proBNP concentrations ranging from 20 pg/mL to 10 ng/mL. The results of the 62 clinical specimens measured by our method showed a good linear correlation with those measured by the Roche E411 kit. Conclusion The new LFA detection method of NT-proBNP levels based on the near-infrared POCT device was rapid and highly sensitive with wide scope and was thus suitable for rapid and early clinical diagnosis of cardiac impairment.

关键词： NT-proBNP Near-infrared POCT device Lateral flow assay

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