检索结果-南通市图书馆

Molecular characterization of GmNHX2,a Na^+/H^+ antiporter gene homolog from soybean,and its heterologous expression to improve salt tolerance in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2009年第19期54卷 3536-3545页

作者： ZHOU GuoAn guan rongxia LI YingHui CHANG RuZhen QIU LiJuan National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Lab of Germplasm & Biotechnology (MOA) Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China

Na+/H+ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. Here, a putative Na+/H+ antiporter gene homolog GmNHX2 from soybean was cloned and predicted to encode a protein of 534 amino acids with 10 putative transmembrane domains. GmNHX2 was expressed in all soybean plant tissues but enriched in roots and its expression was induced by NaCl and polyethylene glycol (PEG) treatments. GmNHX2 exhibits greater sequence similarity with LeNHX2 and AtNHX6 than that of AtNHX1 and AtSOS1. Although phylogenetic analysis clustered GmNHX2 with organellar (tonoplast and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant cells. Furthermore, transgenic Arabidopsis plants expressing GmNHX2 were more tolerant to high NaCl concentrations during germination and seedling stages when compared with wild-type plants. These results suggest that GmNHX2 is a membrane Na+/H+ antiporter and may function to regulate ion homeostasis under salt stress.

关键词：植物耐盐基因工程盐胁迫离子分布植物组织 GmNHX2

Co-regulation of indole glucosinolates and camalexin biosynthesis by CPK5/CPK6 and MPK3/MPK6 signaling pathways^°°

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2020年第11期62卷 1780-1796页

作者： Liuyi Vang Yan Zhang rongxia guan Sen Li Xuwen Xu Shuqun Zhang Juan Xu State Key Lab oratory of Plant Physiology and Biochem istry College of Life SciencesZhejiang UniversityHangzhouZhejiang 310058China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Division of Biochemistry University of MissouriColumbiaMO 65211USA

Secondary plant metabolites,represented by indole glucosinolates(IGS)and camalexin,play important roles in Arabidopsis ***,we demonstrated the importance of MPK3 and MPK6,two closely related MAPKs,in regulating Botrytis cinerea(Bc)-induced IGS and camalexin *** we report that CPK5 and CPK6,two redundant calcium-dependent protein kinases(CPKs),are also involved in regulating the biosynthesis of these secondary *** loss-of-function of both CPK5 and CPK6 compromises plant resistance to *** profiling of CPK5-VK transgenic plants,in which a truncated constitutively active CPK5 is driven by a steroidinducible promoter,revealed that biosynthetic genes of both IGS and camalexin pathways are coordinately upregulated after the induction of CPK5-VK,leading to high-level accumulation of camalexin and 4-methoxyindole-3-ylmethylglucosinolate(4MI3G).Induction of camalexin and 4MI3G,as well as the genes in their biosynthesis pathways,is greatly compromised in cpks cpk6 mutant in response to *** a conditional cpk5 cpk6 mpk3 mpk6 quadruple mutant,Be resistance and induction of IGS and camalexin are further reduced in comparison to either cpks cpk6 or conditional mpk3 mpk6 double mutant,suggesting that both CPK5/CPK6 and MPK3/MPK6 signaling pathways contribute to promote the biosynthesis of 4MI3G and camalexin in defense against Be.

关键词： indole coordinate constitutive

Deep genotyping of the gene GmSNAP facilitates pyramiding resistance to cyst nematode in soybean

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2019年第5期7卷 677-684页

作者： Yu Tian Bo Liu Xuehui Shi Jochen C.Reif rongxia guan Ying-hui Li Li-juan Qiu The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Lab of Germplasm Utilization(MOA) Institute of Crop SciencesChinese Academy of Agricultural Sciences100081 BeijingChina Department of Breeding Research Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)GaterslebenGermany

Soybean cyst nematode(SCN)is a highly destructive *** soybean host genome harbors at least two major genes for resistance(rhg1 and Rhg4),as well as a minor locus(SCN3-11).In the present study,a splicing site in GmSNAP11,the potential causal gene of SCN3-11,was identified by comparison of the GmSNAP11 cDNA sequences generated from resistant and susceptible soybean *** sequence information was used to design a codominant CAPS marker,GmSNAP11-2565,which was used to genotype a panel of 209 soybean accessions varying with respect to SCN *** of the effect of the haplotypes formed by GmSNAP11-2565 and another large-effect(nonsynonymous)locus,GmSNAP11-2307,previously identified in GmSNAP11,revealed linkage disequilibrium(P<0.0001)between the two loci,suggesting that GmSNAP11-2565 could be used as a marker for ***11-2565 was accordingly used,along with established markers for GmSNAP18(rhg1)and GmSHMT(Rhg4),to characterize the panel *** mean SCN female index of accessions carrying only the GmSNAP11 allele associated with resistance(20.3%)was higher than that associated with accessions carrying alleles for resistance at both GmSNAP11 and GmSNAP18(12.4%),while the index for accessions carrying alleles for resistance at all of GmSNAP11,GmSNAP18,and GmSHMT was very low(1.9%).Selection on all three markers was effective for maintaining a high level of resistance to SCN race 3.

关键词： SCN α-SNAP Allelic combination Marker-assisted selection(MAS) Haplotype analysis

Geographical distribution of GmTfl1 alleles in Chinese soybean varieties

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2015年第5期3卷 371-378页

作者： Guifeng Liu Lin Zhao Benjamin J.Averitt Ying Liu Bo Zhang Ruzhen Chang Yansong Ma Xiaoyan Luan rongxia guan Lijuan Qiu Soybean Research Institute Northeast Agricultural University Department of Crop and Soil Environmental Sciences VA Polytechnic Institute and State University The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Institute of Crop ScienceChinese Academy of Agricultural Sciences Heilongjiang Academy of Agricultural Sciences

Stem growth habit is an important agronomic trait in soybean and is subject to artificial selection. This study aimed to provide a theory for genotypic selection of stem growth habit for breeding purposes by analyzing the alleles of Gm Tfl1 gene in Chinese soybean varieties and establishing a database of Gm Tfl1 variation. Using knowledge of insertion and deletion(Indel) in the non-coding region and four single-nucleotide polymorphisms(SNPs) in the coding sequences of the Gm Tfl1 gene, four CAPS and one Indel markers were developed and used to test 1120 Chinese soybean varieties. We found that the dominant Gm Tfl1 allele was prevalent in accessions from the Northern ecoregion, whereas the recessive allele, Gmtfl1, was more common in the Southern ecoregion, and the proportions of Gm Tfl1 and recessive alleles were respectively 40.1% and 59.9% in the Huang-Huai ecoregion. The proportion of Gm Tfl1 decreased and that of Gmtfl1 increased, gradually from north to south. Allele Gm Tfl1-a was present in higher proportions in the Huang-Huai spring, Huang-Huai summer, and Northern spring sub-ecoregions than that in the other sub-ecoregions. Gm Tfl1-b was common in the Northeast spring, Northern spring and Southern summer sub-ecoregions. Gmtfl1-ta was found mainly in the Huang-Huai spring,Huang-Huai summer and Southern spring sub-ecoregions. The Gmtfl1-ab allele was distributed in all six soybean sub-ecoregions. The Gmtfl1-bb allele was distributed mainly in the Huang-Huai spring and summer and Southern spring and summer sub-ecoregions,but the Gmtfl1-tb allele was detected only in the Huang-Huai summer sub-ecoregion. The distributions of Gm Tfl1 and Gmtfl1 have shown no large changes in nearly 60 years of breeding, but the frequency of the recessive genotype Gmtfl1 has shown a rising trend in the last 20 years. This study provides a theoretical foundation for breeding new soybean varieties for different ecoregions.

关键词： Soybean Stem growth habit Gm Tfl1 Distribution

Genetic contribution of foreign germplasm to elite Chinese soybean (Glycine max) cultivars revealed by SSR markers

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2006年第9期51卷 1078-1084页

作者： QIN Jun CHEN Weiyuan guan rongxia JIANG Chengxi LI Yinghui FU Yashu LIU Zhangxiong ZHANG Mengchen CHANG Ruzhen QIU Lijuan National Key Facility of Crop Gene Resources and Genetic Improvement Key Lab of Crop Gerrnplasm Resources & Biotechology (MOA) Institute of Crop Science Chinese Agricultural Academy of Sciences Beijing 100081 China Hebei Academy of Agricultural and Forestry Science Shijiazhuang 050031 China Heilongjiang Academy of Agricultural Science Suihua 152052 China

Simple sequence repeats (SSR) marker analysis, combined with pedigree analysis, was car- ried out to trace the genetic constitutes of Suinong 14 and Hefeng 25 in assessing the contribution of for- eign germplasm to those elite cultivars. The overall goal is to accumulate information for further effective utilization of foreign germplasm in Chinese soybean breeding programs. SSR clustering results indicated that the genetic base of elite Chinese soybean culti- vars, including Suinong 14 and Hefeng 25, were broadened and enhanced via introduction of Amsoy from the U.S. and Shishengchangye from Japan. The pedigree analysis showed a very high coefficient of parentage between Shishengchangye and Hefeng 25, between Shishengchangye and Suinong 14, and between Amsoy and Suinong 14. The genetic simi- larity between Suinong 14 and Hefeng 25 was 60.58%. Among 20 linkage groups, more genomic regions were transferred from Hefeng 25 to Suinong 14 in linkage group (LG) I, L and C2 than other LGs. Among the unique alleles of foreign parents, five specific loci in Amsoy were transferred to Suinong 14, and three loci from Shishengchangye to Suinong 14. Some SSR loci were proved to be correlated with phenotypes: two loci for seed size introgressed from Shishengchangye to Suinong 14, and one locus forprotein content from Amsoy to Suinong 14. These results indicate that the two foreign parents might have important contributions in the development of Suinong 14 and Hefeng 25.

关键词：大豆谱系分析 SSR 序列分析种质资源

Mapping and validation of a dominant salt tolerance gene in the cultivated soybean(Glycine max) variety Tiefeng 8

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2014年第6期2卷 358-365页

作者： rongxia guan Jiangang Chen Jinghan Jiang guangyu Liu Ying Liu Lei Tian Lili Yu Ruzhen Chang Li-juan Qiu The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Key Laboratory of Germplasm & Biotechnology (MOA)Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Salt is an abiotic stress factor that strongly affects soybean growth and production. A single dominant gene has been shown to confer salt tolerance in the soybean cultivar Tiefeng *** objective of the present study was to genetically map the salt-tolerance gene in an F2:3population and a recombinant inbred line(RIL) population derived from a cross between two cultivated soybeans, Tiefeng 8(tolerant) and 85-140(sensitive). The F2:3families and RILs were treated with 200 mmol L-1Na Cl to evaluate salt tolerance. The F2:3population showed 1(42 tolerant): 2(132 segregating): 1(65 sensitive) segregation, indicating a single dominant gene for salt tolerance in Tiefeng 8. A sequence-characterized amplified region(SCAR) marker from a previously identified random amplified polymorphic DNA(RAPD)marker and four insertion/deletion polymorphism(In Del) markers were developed within the mapping region. Using these markers along with SSR markers, the salt-tolerance gene was mapped within 209 kb flanked by SCAR marker QS08064 and SSR marker Barcsoyssr_3_1301 on chromosome 3. Three markers that cosegregated with the salt tolerance gene and SCAR marker QS08064 were used to genotype 35 tolerant and 23 sensitive soybean accessions. These markers showed selection efficiencies of 76.2% to94.2%. The results indicate that these markers will be useful for marker-assisted breeding and facilitating map-based cloning of the salt tolerance gene in soybean.

关键词： Salt tolerance Gene mapping Soybean Glycine max

Characterization of Two GmSALT3 Promoters Involved in Salt Tolerance in Soybean

在线全文

学校读者我要写书评

暂无评论

Characterization of Two GmSALT3 Promoters Involved in Salt T...

Assessing the numbers of SNPs needed to establish molecular IDs and characterize the genetic diversityof soybean cultivars derived from Tokachi nagaha

中国作物学会——2015年学术年会

作者： Lili Yu rongxia guan Lijuan Qiu National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural Sciences

Two cultivar soybean Tiefeng8(salt tolerance)and Mayibao(salt sensitive)belong to haplotypel and haplotype3 respectively were used for the identification and characterization of the promoter regions for the soybean salt tolerance associated gene(GmSALT3).The DNA sequences 5’upstream from the transcriptional start sites of GmSALT3,1748 bp and 1921 bp in length respectively,were *** analysis shows that a total of 45 SNP and 8 InDel were identified.A number of binding sites of the transcription factors respond to stress including ABRE,GATA-BOX,GTl,I-BOX,MYB,MYC and W-BOX were founded,as well as basic element TATA-BOX,CAAT-BOX and enhancer *** were many obvious differences in number of these ***,the binding sites of enhancer element-300CORE and abiotic stress response element ASFl were only found in Tiefeng8 *** investigated the expression pattern of GmSALT3 by quantifying the relative abundance of the mRNA in root of Tiefeng8 and *** expression was much higher in Tiefeng8than Mayibao after 200 mM salt *** conclusion,the bioinformatics analysis suggested that the expression of GmSALT3 was regulated by a variety of factors;however,the mechanisms of the regulation were different between the two cultivar soybeans.

关键词： Bioinformatics analysis Promoter Salt tolerance associated gene Salt Soybean

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The Crop Journal 2017年第4期5卷 326-336页

作者： Zhangxiong Liu Jun Li Xuhong Fan Nang Myint Phyu Sin Htwe Shuming Wang Wen Huang Jiyu Yang Lili Xing Lijun Chen Yinghui Li rongxia guan Ruzhen Chang Dechun Wang Lijuan Qiu National Key Facility for Gene Resources and Genetic Improvement Key Laboratory of Crop Germplasm Utilization Ministry of AgricultureInstitute of Crop Science Chinese Academy of Agricultural Science Beijing 100081 China College of Agriculture Guangxi University Nanning 530004 China Institute of Soybean Research Jilin Academy of Agricultural Sciences Changchun 130124 China Tonghua Academy of Agricultural Sciences Meihekou 135007 Jilin China Jilin City Academy of Agricultural Sciences Jilin 132101 China Beijing Seed Administration Station Beijing 100088 China Department of Plant Soil and Microbial Sciences Michigan State University East Lansing 48824 USA

The development of a core set of SNP molecular markers that could be widely used in soybean genetic research would greatly facilitate research into the genetic diversity of *** conducted an analysis of Tokachi nagaha and 137 of its descendant soybean cultivars using 4044 SNP markers with the goal of determining the appropriate number of single-nucleotide polymorphisms(SNPs)needed to construct unambiguous molecular IDs and characterize genetic diversity based on a genetic distance matrix correlation *** the number of SNPs was held constant,the number of accession pairs that could be distinguished increased as the polymorphism informative content(PIC)value of the SNPs increased.A core panel of 20 selected SNPs from 11 linkage groups with a mean PIC value of 0.3703 and a range of 0.3640–0.3749 was able to identify almost all of the accession pairs in our study[9445 pairs(99.92%)].The eight accession pairs that could not be identified with this core SNP set all originated from the same province and some of them had the same parental *** molecular IDs of the 138 accessions were constructed using the core 20 *** is known that both the number of SNPs and PIC values should be considered when SNPs are selected for use in the analysis of genetic *** this study,when the PIC value was 0.3460,the correlation coefficient between the genetic distance matrices associated with a panel of 200 SNPs and the total population was>0.800,indicating satisfactory *** high-accuracy,high-resolution core SNP panel for germplasm fingerprinting and our findings about assessing genetic diversity will likely markedly improve the management and utilization efficiency of soybean germplasm resources.

关键词： Soybean SNP Genetic diversity Genetic distance Molecular ID

Numerical Methods for a Class of Quadratic Matrix Equations

在线全文

学校读者我要写书评

暂无评论

应用数学 2024年第4期37卷 962-970页

作者： guan Jinrui WANG Zhixin SHAO rongxia School of Mathematics and Statistics Taiyuan Normal UniversityJinzhong 030619China Shanxi Key Laboratory of Intelligent Optimization Computing and Blockchain Technology Taiyuan Normal UniversityJinzhong 030619China School of Statistics and Data Science Xinjiang University of Finance and EconomicsUrumqi 830012China

Quadratic matrix equations arise in many elds of scienti c computing and engineering *** this paper,we consider a class of quadratic matrix *** a certain condition,we rst prove the existence of minimal nonnegative solution for this quadratic matrix equation,and then propose some numerical methods for solving *** analysis and numerical examples are given to verify the theories and the numerical methods of this paper.

关键词： Quadratic matrix equation M-matrix Minimal nonnegative solution Newton method Bernoulli method