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RpL 38 modulates germ cell differentiation by controlling Bam expression in Drosophila testis

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Science China Life Sciences 2024年

作者： Yang Fang Fengchao Zhang Fangzhen Zhao Jiajia Wang Xinkai Cheng fei Ye Jiayu He long Zhao Ying Su Key Laboratory of Evolution & Marine Biodiversity (Ministry of Education) and Institute of Evolution & Marine Biodiversity Ocean University of China College of Marine Life Sciences Ocean University of China Fisheries College Ocean University of China

Switching from mitotic spermatogonia to meiotic spermatocytes is critical to producing haploid sperms during male germ cell differentiation. However, the underlying mechanisms of this switch remain largely unexplored. In Drosophila melanogaster, the gene RpL38encodes the ribosomal protein L38, one component of the 60S subunit of ribosomes. We found that its depletion in spermatogonia severely diminished the production of mature sperms and thus led to the infertility of male flies. By examining the germ cell differentiation in testes,we found that RpL38-knockdown blocked the transition from spermatogonia to spermatocytes and accumulated spermatogonia in the testis. To understand the intrinsic reason for this blockage, we conducted proteomic analysis for these spermatogonia populations. Differing from the control spermatogonia, the accumulated spermatogonia in RpL38-knockdown testes already expressed many spermatocyte markers but lacked many meiosis-related proteins, suggesting that spermatogonia need to prepare some important proteins for meiosis to complete their switch into spermatocytes. Mechanistically, we found that the expression of bag of marbles (bam), a crucial determinant in the transition from spermatogonia to spermatocytes, was inhibited at both the mRNA and protein levels upon RpL38 depletion. We also confirmed that the bam loss phenocopied RpL38 RNAi in the testis phenotype and transcriptomic profiling. Strikingly, overexpressing bam was able to fully rescue the testis abnormality and infertility of RpL38-knockdown flies, indicating that bam is the key effector downstream of RpL38 to regulate spermatogonia differentiation. Overall, our data suggested that germ cells start to prepare meiosis-related proteins as early as the spermatogonial stage, and RpL38 in spermatogonia is required to regulate their transition toward spermatocytes in a bamdependent manner, providing new knowledge for our understanding of the transition process from spermatogonia to spe

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殷商·清商·楚商

南京艺术学院学报（音乐与表演版） 2024年第1期 78-81页

作者：陇菲不详

商者,原为商人之磬。“商音”,乃五帝之遗声,是赫赫有名的商人之音。此《礼记·乐记》所谓“爱者宜歌”“肆直而慈爱”的“商声”,在《史记·乐书》里,被诬为“亡国之声”“靡靡之音”。战国时期“乐戒商音”,是因律学原因而不取“商声... 详细信息

商者,原为商人之磬。“商音”,乃五帝之遗声,是赫赫有名的商人之音。此《礼记·乐记》所谓“爱者宜歌”“肆直而慈爱”的“商声”,在《史记·乐书》里,被诬为“亡国之声”“靡靡之音”。战国时期“乐戒商音”,是因律学原因而不取“商声”,与政事无关。“清商”特色之音是太簇升高一律(半音)的夹钟商,后泛指汉魏六朝乃至隋唐之“清商”“清乐”,一度成为华夏正声。见之于古琴曲的“楚商”,是一个非常奇特的六声调式音阶,至今湖南仍有使用这个调式的民歌。“楚商”除调式音阶之义外,又泛指楚地之乐。楚地之清商、楚商、侧商等,成为魏晋隋唐中国音乐主调。迄今西北戏曲、民歌多使用楚乐侧调之“苦音”,也常常与“花音”交替使用。

关键词：殷商清商楚商商乐商声侧商

时奏宫角杂之以徵羽——兼析清华简《五音图》乐制

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人民音乐 2024年第4期 63-67页

作者：陇菲不详

根据一系列文献记载以及实践传统,保守上古“律居阴而治阳”乐制的古琴家,将五运四宫纪之以三之十二吕律,结晶物化,构成一种以阴阳一体之宫为中介,“时奏宫角,杂之以徵羽”^(1),“弹羽角应,弹宫徵应”^(2),“徐动宫徵,微挥角羽”^(3)的... 详细信息

根据一系列文献记载以及实践传统,保守上古“律居阴而治阳”乐制的古琴家,将五运四宫纪之以三之十二吕律,结晶物化,构成一种以阴阳一体之宫为中介,“时奏宫角,杂之以徵羽”^(1),“弹羽角应,弹宫徵应”^(2),“徐动宫徵,微挥角羽”^(3)的乐制。

关键词：乐制清华简十二吕律四宫纪之以三

Systematic identification of wheat spike developmental regulators by integrated multiomics, transcriptional network, GWAS, and genetic analyses

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Molecular Plant 2024年第3期17卷 438-459页

作者： Xuelei Lin Yongxin Xu Dongzhi Wang Yiman Yang Xiaoyu Zhang Xiaomin Bie Lixuan Gui Zhongxu Chen Yiliang Ding long Mao Xueyong Zhang fei Lu Xiansheng Zhang Cristobal Uauyo Xiangdong Fu Jun Xiao Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Nanjing Agricultural University NanjingJiangsu 210095China Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTaianShandong 271018China Department of Life Science Tcuni Inc.ChengduSichuan 610000China John Innes Centre Norwich Research ParkNorwich NR47UHUK Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China CAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS) Institute of Genetics and Developmental BiologyCASBeijing 100101China

The spike architecture of wheat plays a crucial role in determining grain number,making it a key trait for optimization in wheat breeding *** this study,we used a multi-omic approach to analyze the transcriptome and epigenome profiles of the young spike at eight developmental stages,revealing co-ordinated changes in chromatin accessibility and H3K27me3 abundance during the flowering *** constructed a core transcriptional regulatory network(TRN)that drives wheat spike formation and experimentally validated a multi-layer regulatorymodule involving TaSPL15,TaAGLG1,and *** integrating the TRN with genome-wide association studies,we identified 227 transcription factors,including 42 with known functions and 185 with unknown *** investigation of 61 novel transcription factors using multiple homozygous mutant lines revealed 36 transcription factors that regulate spike architecture or flowering time,such as TaMYC2-A1,TaMYB30-A1,and *** particular interest,TaMYB30-A1,downstream of and repressed by WFzP,was found to regulate fertile spikelet ***,the excellent haplotype of TaMYB30-A1,which contains a C allele at the WFzP binding site,was enriched during wheat breeding improvement in China,leading to improved agronomic ***,we constructed a free and open access Wheat Spike Multi-Omic Database(http://39.***.48.156:8800/#/).Our study identifies novel and high-confidence regulators and offers an effective strategy for dissecting the genetic basis of wheat spike development,with practical value forwheat breeding.

关键词： wheat spike development epigenome TRN TaMYB30-A1 breeding selection

Asymmetric Drying and Wetting Trends in Eastern and Western China

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Advances in Atmospheric Sciences 2024年第2期41卷 221-232页

作者： Wen WU fei JI Shujuan HU Yongli HE College of Atmospheric Sciences Lanzhou UniversityLanzhou 730000China Collaborative Innovation Center for Western Ecological Safety Lanzhou UniversityLanzhou 730000China

As an important factor that directly affects agricultural production, the social economy, and policy implementation,observed changes in dry/wet conditions have become a matter of widespread concern. However, previous research has mainly focused on the long-term linear changes of dry/wet conditions, while the detection and evolution of the non-linear trends related to dry/wet changes have received less attention. The non-linear trends of the annual aridity index, obtained by the Ensemble Empirical Mode Decomposition(EEMD) method, reveal that changes in dry/wet conditions in China are asymmetric and can be characterized by contrasting features in both time and space in China. Spatially, most areas in western China have experienced transitions from drying to wetting, while opposite changes have occurred in most areas of eastern China. Temporally, the transitions occurred earlier in western China compared to eastern China. Research into the asymmetric spatial characteristics of dry/wet conditions compensates for the inadequacies of previous studies, which focused solely on temporal evolution;at the same time, it remedies the inadequacies of traditional research on linear trends over centennial timescales. Analyzing the non-linear trend also provides for a more comprehensive understanding of the drying/wetting changes in China.

关键词： aridity index EEMD dry/wet conditions asymmetric evolution

Could the EU carbon border adjustment mechanism promote climate mitigation?An economy-wide analysis

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Advances in Climate Change Research 2024年第3期15卷 557-571页

作者： Kun ZHANG Yun-fei YAO Xiang-Yan QIAN Yu-fei ZHANG Qiao-Mei LIANG Yi-Ming WEI College of Economics and Management Beijing University of TechnologyBeijing 100124China Center for Energy and Environmental Policy Research Beijing Institute of TechnologyBeijing 100081China School of Management and Economics Beijing Institute of TechnologyBeijing 100081China Beijing Key Laboratory of Energy Economics and Environmental Management Beijing 100081China Sinopec Research Institute of Petroleum Engineering SinopecBeijing 100101China College of Economics and Management Nanjing Agricultural UniversityNanjing 210095China

Due to concerns about carbon leakage and sectoral competitiveness,the European Union(EU)proposed implementing the carbon border adjustment mechanism(CBAM).The effectiveness and potential negative consequences of CBAM have aroused extensive *** the perspective of the economy-wide analysis,this study uses a global computable general equilibrium model to explore the rationality of CBAM from the aspects of socioeconomic impact and the effects of promoting climate ***,the potential alternative mechanism of CBAM is *** results show that CBAM can reduce the EU's gross domestic product(GDP)loss;however,the GDP loss in all other regions ***,CBAM raises household welfare losses in most regions,including the ***,although CBAM can reduce the marginal abatement cost in eight regions,it comes at the cost of greater economic ***,the economic and household welfare cost of raising emissions reduction targets in regions like the USA and Japan is substantially higher than the impact of passively accepting the CBAM;therefore,CBAM's ability to drive ambitious emission reduction initiatives may be ***,for the potential alternative mechanism,from the perspective of reducing economic cost and household welfare losses,the EU could implement domestic tax cuts in the short-term and promote global unified carbon pricing in the long-term.

关键词： Border adjustment mechanism Computable general equilibrium model Carbon price Climate policy Global carbon pricing

TOPK Inhibition Enhances the Sensitivity of Colorectal Cancer Cells to Radiotherapy by Reducing the DNA Damage Response

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Current Medical Science 2024年第3期44卷 545-553页

作者： Shi-gui PANG Xin ZHANG Zhao-xin LI Li-fei HE Feng CHEN Ming-long LIU Ying-ze HUANG Jian-mei MO Kong-lan LUO Juan-juan XIAO Feng ZHU Department of Oncology The Affiliated Hospital of Guilin Medical UniversityGuilin541001China Cancer Research Institute The Affiliated Hospital of Guilin Medical UniversityGuilin541001China Translational Medical Center Huaihe HospitalHenan UniversityKaifeng475000China guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair The Affiliated Hospital of Guilin Medical UniversityGuilin541001China

Objective Abnormal expression of T-lymphokine-activated killer cell-originated protein kinase(TOPK)was reported to be closely related to the resistance of prostate cancer to radiotherapy and to targeted drug resistance in lung ***,the role of TOPK inhibition in enhancing radiosensitivity of colorectal cancer(CRC)cells is *** study aimed to evaluate the radiosensitization of TOPK knockdown in CRC *** The expression of TOPK was detected in CRC tissues by immunohistochemistry,and the effect of TOPK knockdown was detected in CRC cells by Western ***-8 and clonogenic assays were used to detect the growth and clonogenic ability of CRC cells after TOPK knockdown combined with radiotherapy in CRC ***,proteomic analysis showed that the phosphorylation of TOPK downstream proteins changed after *** damage was detected by the comet *** in the DNA damage response signaling pathway were analyzed by Western blotting,and apoptosis was detected by flow *** The expression of TOPK was significantly greater in CRC tissues at grades 2–4 than in those at grade *** irradiation,CRC cells with genetically silenced TOPK had shorter comet tails and reduced expression levels of DNA damage response-associated proteins,including phospho-cyclin-dependent kinase 1(p-CDK1),phospho-ataxia telangiectasia-mutated(p-ATM),poly ADP-ribose polymerase(PARP),and meiotic recombination 11 homolog 1(MRE11).Conclusions TOPK was overexpressed in patients with moderately to poorly differentiated ***,TOPK knockdown significantly enhanced the radiosensitivity of CRC cells by reducing the DNA damage response.

关键词： T-lymphokine-activated killer cell-originated protein kinase colorectal cancer DNA damage response radiosensitivity

Two polyoxovanadoborates with over 100 nuclei assembled from a {VCoB} cluster

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Science China Chemistry 2024年

作者： Shuang-Xue Wu Yu-Jiao Dong Yang Yang Chao Qin Chun-Yi Sun Wei guan Xin-long Wang Zhong-Min Su Key Laboratory of Polyoxometalate and Reticular Material Chemistry of Ministry of Education Northeast Normal University School of Chemistry and Environmental Engineering Changchun University of Science and Technology Key Laboratory of Advanced Materials of Tropical Island Resources Hainan University

Combining theoretical prediction and experimental exploration,the first sandwich-type polyoxovanadoborate (POVB) with an odd number of nuclei,[V11B26O73(OH)10]8-(1),was discovered under hydrothermal ***-functional theory (DFT)gives a rationalization for the existence of this structure and the structural feature is consistent with the{VNB2N+X}(X=2,4,6,8)general ***,when Co and 1,10-phenanthroline (phen) are introduced into the synthesis of vanadoborates system,a 100-nuclei[Co6(phen)10(H2O)8][V14Co12B72O144(OH)20]·28H2O (2) and a 102-nuclei[Co6(phen)14(H2O)4][V14Co12B72O144(OH)20]·23H2O (3) with the same core structure of[V14Co12B72]12-are *** vase-shaped[V14Co12B72]polyanion is a multilayered sandwich-type structure containing seven distinct structural layers and is the largest known ***,2demonstrates exceptional thermal and chemical stability and serves as an efficient and stable catalyst for the photoreduction of carbon dioxide.

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Minimal Context-Switching Data Race Detection with Dataflow Tracking

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Journal of Computer Science & Technology 2024年第1期39卷 211-226页

作者：郑龙李洋辛杰刘海峰郑然廖小飞金海 National Engineering Research Center for Big Data Technology and System School of Computer Science and Technology Huazhong University of Science and TechnologyWuhan 430074China Services Computing Technology and System Laboratory School of Computer Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China Cluster and Grid Computing Laboratory School of Computer Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China

Data race is one of the most important concurrent anomalies in multi-threaded *** con-straint-based techniques are leveraged into race detection,which is able to find all the races that can be found by any oth-er sound race ***,this constraint-based approach has serious limitations on helping programmers analyze and understand data ***,it may report a large number of false positives due to the unrecognized dataflow propa-gation of the ***,it recommends a wide range of thread context switches to schedule the reported race(in-cluding the false one)whenever this race is exposed during the constraint-solving *** ad hoc recommendation imposes too many context switches,which complicates the data race *** address these two limitations in the state-of-the-art constraint-based race detection,this paper proposes DFTracker,an improved constraint-based race detec-tor to recommend each data race with minimal thread context ***,we reduce the false positives by ana-lyzing and tracking the dataflow in the *** this means,DFTracker thus reduces the unnecessary analysis of false race *** further propose a novel algorithm to recommend an effective race schedule with minimal thread con-text switches for each data *** experimental results on the real applications demonstrate that 1)without removing any true data race,DFTracker effectively prunes false positives by 68%in comparison with the state-of-the-art constraint-based race detector;2)DFTracker recommends as low as 2.6-8.3(4.7 on average)thread context switches per data race in the real world,which is 81.6%fewer context switches per data race than the state-of-the-art constraint based race ***,DFTracker can be used as an effective tool to understand the data race for programmers.

关键词： data race satisfiability modulo theory multi-threaded program dynamic detection