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Effects of tacrolimus on infection of Friend murine leukemia virus to Fυ-4 gene heterozygous mice

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Acta Pharmacologica Sinica 2004年第2期25卷 82-86页

作者： Feng-min ZHANG2, Bao-feng YANG3, Hong-xi gu, Xiao-bei CHEN, Zhao-hua ZHONG, Zhi CHENG Department of Microbiology Department of Pharmacology, Harbin Medical University, Harbin 150086, China 3 Department of Microbiology Harbin Medical University Harbin 150086 Department of Pharmacology Harbin Medical University Harbin 150086

AIM: To investigate the effect of tacrolimus (FK506) on the infection of Friend murine leukemia virus (Friend MuLV) in vivo. METHODS: Three kinds of mice were used including Friend MuLV-sensitive BALB/c mice, Friend MuLV-resistant Fv-4 gene-homozygous mice (Fv-4 mice), and Friend MuLV-resistant Fv-4 gene-heterozygous mice (F1 mice). Tacrolimus was administrated ip to those mice in every 2 d. Those treated mice were inoculated ip with Friend MuLV once on d 3. The symptoms and viral proliferations in those mice were observed to recognize the Friend MuLV infection. The expression and genotype of Fv-4 gene that resistant against the infection of Friend MuLV were analyzed to confirm the genomic background and related mechanism of the resistance. RESULTS: BALB/c mice and F1 mice, but not Fv-4 mice, appeared obvious early death, spleenomegaly, and viral proliferation after both treatments of viral inoculation and tacrolimus administration, whereas the expression and genotype of Fv-4 gene was not changed in F1 mice and Fv-4 mice with treatment of tacrolimus. Compared to the virus- inoculated control, the Friend MuLV-sensitivity of tacrolimus-treated BALB/c mice and the Friend MuLV-resistance of tacrolimus-treated Fv-4 mice were the same as the controls, but only F1 mice became the symptoms and viral proliferation after both treatments. It suggested the Friend MuLV-resistant F1 mice could be converted to be Friend MuLV-sensitive by treatment of tacrolimus, and this conversion was not depended on the expression and genotype of Fv-4 gene. CONCLUSION: Tacrolimus could not inhibit the infection of Friend MuLV in all mice, furthermore, it could enhance the infection of Friend MuLV in F1 mice. The enhancement may be related to the immunosuppres- sive effect of tacrolimus.

关键词： tacrolimus murine leukemia virus transgenic mice

视黄醇对LPS诱发的原代培养大鼠乳腺上皮细胞炎症反应的调节

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畜牧兽医学报 2012年第4期43卷 627-633页

作者：顾蓓蓓苗晋锋卢劲晔邹思湘南京农业大学动物医学院南京210095 泰州出入境检验检疫局泰州225300

本研究旨在优化大鼠乳腺上皮细胞培养体系,建立LPS诱发的炎症反应模型,研究视黄醇对其炎症反应的调节机制。采用胶原酶与透明质酸酶联合消化分离大鼠乳腺上皮细胞。待细胞铺满整个培养瓶的90%时,(1)用不同浓度的LPS处理乳腺上皮细胞,24... 详细信息

本研究旨在优化大鼠乳腺上皮细胞培养体系,建立LPS诱发的炎症反应模型,研究视黄醇对其炎症反应的调节机制。采用胶原酶与透明质酸酶联合消化分离大鼠乳腺上皮细胞。待细胞铺满整个培养瓶的90%时,(1)用不同浓度的LPS处理乳腺上皮细胞,24h收集细胞及培养上清液;(2)分为试验组(添加1μmol.L-1视黄醇)和对照组,处理24h后更换培养液,用10μ***-1的LPS处理细胞,分别于不同时间点收集细胞。结果表明:LPS处理大鼠乳腺上皮细胞后引起各个时间点炎性因子mRNA表达极显著升高,视黄醇能显著下调上述炎性细胞因子的表达。视黄醇预处理能减轻LPS诱发的大鼠乳腺上皮细胞的炎症损伤。

关键词：脂多糖乳腺上皮细胞视黄醇炎性反应

A Multi-center,Randomized,Controlled and Open Clinical Trial of Heyan Kuntai Capsule (和颜坤泰胶囊) and Hormone Therapy in Perimenopausal Women

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Chinese Journal of Integrative Medicine 2018年第7期24卷 487-493页

作者： SUN Ai-jun WANG Ya-ping gu bei ZHENG Ting-ping LIN Shou-qing BAI Wen-pei WEI Yang ZHANG Shao-fen ZHANG Ying Department of Gynecology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Department of Obstetrics and Gynecology Beijing Shijitan Hospital Department of Gynecology Obstetrics and Gynecology Hospital of Fudan University

Objective： To evaluate the efficacy and safety of Heyan Kuntai Capsule（和颜坤泰胶囊, HYKT） and hormone therapy（HT） on perimenopausal syndromes（PMSs）. Methods： From 2005 to 2008, 390 women with PMSs were recruited from 4 clinic centers. The inclusion criteria included ages 40 to 60 years, estradiol（E2） below 30 ng/L, and follicle stimulating hormone（FSH） above 40 IU/L, etc. The patients were randomly assigned to HYKT group or HT group by random number table method, administrated HYKT or conjugated estrogen with/without medroxyprogesterone acetate tablets for 12 months. During treatment, the patients were interviewed quarterly, Kupperman Menopausal Index（KMI） scores, hot flush scores, insomnia scores, Menopause-Specific Quality of Life（MENQOL） scores and adverse effects were used for evaluating drug efficacy and safety respectively. The last interview was made at the end of 12-month treatment. Results： After treatment, KMI scores of HYKT group and HT group were both significantly decreased compared with baseline（P〈0.01） and there was no significant difference between groups（P〉0.05）, except that KMI of HYKT group was higher after 3-month treatment（P〈0.05）. After treatment, hot flush and insomnia scores were both improved significantly in two groups（P〈0.01）; and HT had a better performance than HYKT in improving hot flush（P〈0.05）. MENQOL were significantly improved in both groups after treatment（P〈0.01）; but there was no significant difference between two groups（P〉0.05）. The incidence of adverse event in the HYKT group was much lower than that in the HT group（P〈0.01）. Conclusions： HYKT could effectively relieve PMSs and improve patient quality of life without severe adverse reactions. Although HYKT exerted curative effects more slowly than hormone, it possessed better safety profile than hormone.

关键词： perimenopausal syndromes Heyan Kuntai Capsule hormone therapy Kupperman Menopausal Index Menopause-Specific Quality of Life Chinese medicine

HCA-PCA法在水环境质量综合评价中的应用

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中国农村水利水电 2009年第9期 49-51页

作者：李川顾蓓蕾南京林业大学江苏省林业生态工程重点实验室南京210037 无锡市锡山区环境科学研究所有限公司江苏无锡214101

应用系统聚类分析方法(HCA,Hierarchical Cluster Analysis)和主成分分析方法(PCA,Principal ComponentAnalysis)有机结合而成的HCA-PCA法对南京市雨花台区板桥河流域的环境污染现状进行分析,将区域水环境质量按月份污染轻重进行分类,... 详细信息

应用系统聚类分析方法(HCA,Hierarchical Cluster Analysis)和主成分分析方法(PCA,Principal ComponentAnalysis)有机结合而成的HCA-PCA法对南京市雨花台区板桥河流域的环境污染现状进行分析,将区域水环境质量按月份污染轻重进行分类,确定了污染较重的月份是4月、6月和10月,再进一步明确该区域污染因子为石油类,并提出相应的水环境管理建议。

关键词：系统聚类分析法主成分分析法水环境

Protective effect of retinoid against endotoxin-induced mastitis in rats

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Protective effect of retinoid against endotoxin-induced mast...

中国生物化学与分子生物学会农业生物化学与分子生物学分会...

作者： bei-bei gu Jin-Feng Miao Yu-Mm Zhu Yu’-E Deng Si-xiang Zou College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China

Objective:A lipopolysaccharide (LPS) induced mastitis model in rats was used to study the protective effect of *** and methods:Commencing at gestation day 10,retinoid (dissolved in olive oil) or an equal volume of olive oil wasadministered to rats daily by gavage until *** or pyrogen-free physiological saline was inoculated into the mammary gland

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高性能计算机系统及平台发展状况分析

计算机工程与科学 2013年第11期35卷 6-13页

作者：迟学斌顾蓓蓓武虹王龙朱鹏中国科学院计算机网络信息中心北京100190

近年来,随着国家级高性能计算平台的相继建立,超级计算中心的运维模式也从过去强调购置开销向注重运营管理成本转变。在分析相关技术与应用发展趋势的同时,从高性能计算机系统、芯片等硬件设施和超级计算中心自身及应用等软环境两方面... 详细信息

近年来,随着国家级高性能计算平台的相继建立,超级计算中心的运维模式也从过去强调购置开销向注重运营管理成本转变。在分析相关技术与应用发展趋势的同时,从高性能计算机系统、芯片等硬件设施和超级计算中心自身及应用等软环境两方面对超级计算中心发展现状做出分析,探讨制约超级计算中心可持续发展的相关问题。

关键词：高性能计算机硬件系统软环境超级计算中心协同设计

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当今美术专业毕业生应有水平构想

湖北美术学院学报 2005年第4期8卷 32-33页

作者：顾蓓江汉大学艺术学院

随着时代的进步,经济的迅猛发展,社会对专业人才提出了新的更高要求,为适应当今社会的需求,美术专业毕业生,一方面应该具有多学科优势与艺术技能特长,基础实、学科宽、能力强、使所学知识,技能与社会需要有机结合的专业素质;另一方面还... 详细信息

随着时代的进步,经济的迅猛发展,社会对专业人才提出了新的更高要求,为适应当今社会的需求,美术专业毕业生,一方面应该具有多学科优势与艺术技能特长,基础实、学科宽、能力强、使所学知识,技能与社会需要有机结合的专业素质;另一方面还应具备良好的个人素质,完善的人格、健康的心理、良好的品质、以及与人合作和沟通的能力等。具备这些综合素质复合型美术人才在众多的美术专业毕业生中才能具有竞争的实力,找到理想的职业。

关键词：美术专业毕业生能力综合素质

47例甲状腺手术方法改进以减轻切口疤痕的临床体会

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安徽卫生职业技术学院学报 2009年第6期8卷 37-38页

作者：顾蓓昆山市第二人民医院外科江苏215300

目的:探讨对甲状腺手术方法的改进,能否达到术中术野显露充分、手术安全、手术时间短、术后切口低小及美学效果好的效果。方法:对47例甲状腺手术,注重微创观念,与常规手术方法作三点改进,一是切口的精确设计,二是缝合技术的改进,三是引... 详细信息

目的:探讨对甲状腺手术方法的改进,能否达到术中术野显露充分、手术安全、手术时间短、术后切口低小及美学效果好的效果。方法:对47例甲状腺手术,注重微创观念,与常规手术方法作三点改进,一是切口的精确设计,二是缝合技术的改进,三是引流的放置。结果:47例均治愈,无1例发生喉返神经、甲状旁腺损伤及术后出血,随访4～12个月,无1例复发,无1例疤痕高于皮面,且47例疤痕颜色呈肤色,视觉效果良好。于术后第3～6天痊愈出院,住院时间5～7天。结论:甲状腺的改良切除手术,具有手术创伤减少、手术时间缩短、切口疤痕粘连发生率明显降低等优点,值得临床推广。

关键词：甲状腺手术切口疤痕

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气体电子倍增器的研制及性能测试

核电子学与探测技术 2005年第5期25卷 511-514页

作者：张明龙夏义本王林军顾蓓蓓汪琳杨莹上海大学材料科学与工程学院上海201800

气体电子倍增器(GEM)以其独特的性能在辐射探测器领域得到了广泛的应用,对50μm厚聚酰亚胺(kapton)薄膜利用真空热蒸发和激光掩膜打孔法制作GEM膜,孔径100μm,孔距223μm,并封装流气式探测器,有效探测面积3mm×3mm。5.9keV55F e X射线... 详细信息

气体电子倍增器(GEM)以其独特的性能在辐射探测器领域得到了广泛的应用,对50μm厚聚酰亚胺(kapton)薄膜利用真空热蒸发和激光掩膜打孔法制作GEM膜,孔径100μm,孔距223μm,并封装流气式探测器,有效探测面积3mm×3mm。5.9keV55F e X射线测量了GEM在不同高压和混合气体比例时的脉冲幅度分布情况。讨论了高压和气体比例对探测器计数率和能量分辨率的影响。结果表明GEM具有较高的信噪比,能量分辨率可达18.2%。

关键词：气体电子倍增器辐射探测器聚酰亚胺薄膜 X射线