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Journal of Ocean University of China 2024年第6期23卷 1666-1676页

作者： JIANG Yaohan ZHANG Xue LIU Dan TANG Xianglin gong ruitao School of Marine Sciences Ningbo UniversityNingbo 315800China Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Yantai Institute China Agricultural UniversityYantai 264670China

Ciliates are eukaryotic unicellular organisms with complex morphology and developmental processes,including asexual and sexual *** is a form of sexual process that renews genetic ***,visualizing conjugation in ciliates is a challenge due to the complexity and dynamics of the process,while traditional staining methods are often insufficient for the *** study introduces a new method for visualizing developmental progression in the nuclei during conjugation using Hoechst33342 *** describes how to proceed from cell culture,conjugation induction and synchronization,staining preparation,and observation to statistical *** combination of fluorescent staining with the‘volume-fixing'technique eliminates the fixation and dehydration steps,thus reducing the overall operation time to just 20 *** method offers several advantages over traditional staining techniques for studying the nuclei during *** improves image quality and workflow efficiency and enables real-time observation of live cell *** solutions to challenges that may arise during experimental procedures are introduced and references and guidelines for cytological research are provided in this paper.

关键词： ciliates conjugation nucleus cytology Hoechst33342 staining

Nuclear events during conjugation in the poorly studied model ciliate Paramecium jenningsi

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Water Biology and Security 2023年第4期2卷 69-77页

作者： Xue Zhang ruitao gong Yaohan Jiang Xiaoteng Lu Chenyin Wu Lihan Wang Hongwei Ma Zhengxiang Zhang Weibo Song Khaled A.S.Al-Rasheid Adriana Vallesi Feng Gao Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao266003China Department of Biology Shenzhen MSU-BIT UniversityShenzhen518172China College of Fisheries Ocean University of ChinaQingdao266003China Laoshan Laboratory Qingdao266237China Zoology Department College of ScienceKing Saud UniversityRiyadh11451Saudi Arabia Laboratory of Eukaryotic Microbiology and Animal Biology University of CamerinoCamerino62032Italy

Ciliated protists are highly differentiated unicellular eukaryotes that possess special sexual processes(conjugation and autogamy)that rely on their unique nuclear dimorphism,i.e.,the presence of both a germline micronucleus(MIC)and a somatic macronucleus(MAC)in a single *** sexual processes show a high diversity in different *** better understand the differentiation and evolution of sexual processes in closely related species,we investigated the nuclear events during conjugation in Paramecium jenningsi strain GZ,a poorly studied close relative of the well-known *** *** main results include:(1)the conjugation process takes about 48 h,including three prezygotic divisions(meiosis I,II,and mitosis)and three postzygotic divisions;(2)the MICs are dominated by the“parachute”phase at the prophase of meiosis I;(3)after meiosis II,a variable number of nuclei undergo the third prezygotic division,and the two products near the paroral cone become the genetically identical migratory and stationary pronuclei;(4)the synkaryon divides three times to form the MIC and the MAC anlagen,but only one nuclear product of the first postzygotic division completes the next two divisions;and(5)an extra cell division is required to complete the last step of conjugation,during which two MIC anlagen undergo mitotic division while two MAC anlagen are distributed between the daughter cells without *** comparison of the nuclear events during conjugation in representatives of the class Oligohymenophorea reveals that usually there are three prezygotic divisions and a variable number of postzygotic ***,the number of selected and differentiated nuclei after each division differs among *** study provided a new model ciliate for further investigations of nuclear selection and differentiation as well as nuclear morphology during meiosis.

关键词： Ciliates Conjugation Cytology Life cycle Nuclear events Paramecium jenningsi

基于多目标遗传算法的云服务系统资源调度失效局部最优感知方法

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长江信息通信 2024年第8期37卷 21-23页

作者：龚瑞涛迈韦尼通讯技术(上海)有限公司上海200050

针对当前资源调度失效感知效果不佳,可靠性和感知灵敏度的问题,提出基于多目标遗传算法的云服务系统资源调度失效局部最优感知方法,结合多目标遗传算法,通过资源调度的失效特征规律识别,对资源调度的失效特征规律识别方法优化,抽取受信... 详细信息

针对当前资源调度失效感知效果不佳,可靠性和感知灵敏度的问题,提出基于多目标遗传算法的云服务系统资源调度失效局部最优感知方法,结合多目标遗传算法,通过资源调度的失效特征规律识别,对资源调度的失效特征规律识别方法优化,抽取受信任的云计算资源,识别和筛查资源失效特征归类,并对局部调度失效资源进行定位,构建云服务系统资源调度失效局部最优感模型,求解云资源局部调度失效区域定位规则。实验结果表明,该方法具有较高的可靠性和感知灵敏度,保证云服务系统资源调度失效感知结果的有效性。

关键词：多目标遗传算法云服务系统资源调度局部最优感知

Running safety assessment of a train traversing a three-tower cable-stayed bridge under spatially varying ground motion

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Railway Engineering Science 2020年第2期28卷 184-198页

作者： Wei gong Zhihui Zhu Yu Liu ruitao Liu Yongjiu Tang Lizhong Jiang Shool of Civil Engineering Central South UniversityChangsha 410075China National Engineering Laboratory for High-Speed Railway Construction Changsha 410075China

To explore the influence of spatially varying ground motion on the dynamic behavior of a train passing through a three-tower cable-stayed bridge,a 3D train–track–bridge coupled model is established for accurately simulating the train–bridge interaction under earthquake excitation,which is made up of a vehicle model built by multi-body dynamics,a track–bridge finite element model,and a 3D rolling wheel–rail contact model.A conditional simulation method,which takes into consideration the wave passage effect,incoherence effect,and site-response effect,is adopted to simulate the spatially varying ground motion under different soil *** multi-time-step method previously proposed by the authors is also adopted to improve computational *** dynamic responses of the train running on a three-tower cablestayed bridge are calculated with differing earthquake excitations and train *** results indicate that(1)the earthquake excitation significantly increases the responses of the train–bridge system,but at a design speed,all the running safety indices meet the code requirements;(2)the incoherence and site-response effects should also be considered in the seismic analysis for long-span bridges though there is no fixed pattern for determining their influences;(3)different train speeds that vary the vibration characteristics of the train–bridge system affect the vibration frequencies of the car body and bridge.

关键词： Earthquake Spatially varying ground motion Long-span bridges Nonlinear wheel-rail contact Running safety

Superconductivity in Kagome Metal YRu_(3)Si_(2)with Strong Electron Correlations

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Chinese Physics Letters 2022年第8期39卷 59-64页

作者： Chunsheng gong Shangjie Tian Zhijun Tu Qiangwei Yin Yang Fu ruitao Luo Hechang Lei Laboratory for Neutron Scattering and Beijing Key Laboratory of Optoelectronic Functional Materials&MicroNano DevicesDepartment of PhysicsRenmin University of ChinaBeijing 100872China

We report the detailed physical properties of YRu_(3)Si_(2)with the Ru kagome lattice at normal and superconducting *** results of resistivity and magnetization show that YRu_(3)Si_(2)is a type-II bulk superconductor with T_(c)~3.0 *** specific heat measurement further suggests that this superconductivity could originate from the weak or moderate electron-phonon *** the other hand,both large Kadawaki–Woods ratio and Wilson ratio indicate that there is a strong electron correlation effect in this system,which may have a connection with the featured flat band of kagome lattice.

关键词： Strong (3) Electron

Pressure-induced superconductivity at 32 K in MoB2

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National Science Review 2023年第5期10卷 215-220页

作者： Cuiying Pei Jianfeng Zhang Qi Wang Yi Zhao Lingling Gao Chunsheng gong Shangjie Tian ruitao Luo Mingtao Li Wenge Yang Zhong-Yi Lu Hechang Lei Kai Liu Yanpeng Qi School of Physical Science and Technology Shanghai Tech University Department of Physics and Beijing Key Laboratory of Opto-electronic Functional Materials&Micro-nano Devices Renmin University of China Shanghai Tech Laboratory for Topological Physics Shanghai Tech University Center for High Pressure Science and Technology Advanced Research Shanghai Key Laboratory of High-resolution Electron Microscopy Shanghai Tech University

Since the discovery of superconductivity in MgB2(Tc～ 39 K),the search for superconductivity in related materials with similar structures or ingredients has never *** about 100 binary borides have been explored,only a few of them show superconductivity with relatively low *** this work,we report the discovery of superconductivity up to 32 K,which is the highest Tcin transition-metal borides,in MoB2under *** Tcof MoB2in the α phase can be well explained by theoretical calculations in the framework of electron-phonon ***,the coupling between the d electrons of Mo and the out-of-plane Mo-phonon modes are the main driving force of the 32 K superconductivity of *** study sheds light on the exploration of high-Tcsuperconductors in transition metal borides.

关键词： superconductivity high pressure borides