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Identification of a novel caspase cleavage motif AEAD

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Virologica Sinica 2024年第5期39卷 755-766页

作者： Yujie Fang zhou gong Miaomiao You Ke Peng State Key Laboratory of Virology Center for Antiviral ResearchCenter for Biosafety Mega-ScienceWuhan Institute of VirologyChinese Academy of SciencesWuhan430207China University of Chinese Academy of Sciences Beijing100049China State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics Innovation Academy for Precision Measurement Science and Technology Chinese Academy of SciencesWuhan430071China Provincial Key Laboratory of Jiangxia Wuhan430207China

Infections of many viruses induce caspase activation to regulate multiple cellular pathways,including programmed cell death,immune signaling and *** of caspase cleavage sites and substrates are important for understanding the regulation mechanisms of caspase ***,we identified and analyzed a novel caspase cleavage motif AEAD,and confirmed its caspase dependent cleavage activity in natural substrate,such as nitric oxide-associated protein 1(NOA1).Fusing the enhanced green fluorescent protein(EGFP)with the mitochondrial marker protein Tom20 through the AEAD motif peptide localized EGFP to the *** the activation of caspase triggered by Sendai virus(SeV)or herpes simplex virus type 1(HSV-1)infection,EGFP diffusely localized to the cell due to the caspase-mediated cleavage,thus allowing visual detection of the virusinduced caspase *** AEAD peptide-derived inhibitor Z-AEAD-FMK were developed,which significantly inhibited the activities of caspases-1,-3,-6,-7,-8 and-9,exhibiting a broad caspase inhibition *** inhibitor further prevented caspases-mediated cleavage of downstream substrates,including BID,PARP1,LMNA,pro-IL-1β,pro-IL-18,GSDMD and GSDME,protecting cells from virus-induced apoptotic and pyroptotic cell ***,our findings provide a new perspective for the identification of novel caspase cleavage motifs and the development of new caspase inhibitors and anti-inflammatory drugs.

关键词： Caspase AEAD motif Inhibitor Virus Cell death

Optimization of Multi-Project Multi-Site Location Based on MOPSOs

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Journal of China University of Mining and Technology 2006年第2期16卷 167-170页

作者： ZHANG Yong gong Dun-wei zhou Yong School of Information and Electronic Engineering China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China

Multi-project multi-site location problems are multi-objective combinational optimization ones with discrete variables which are hard to solve. To do so, the case of particle swarm optimization is considered due to its useful char- acteristics such as easy implantation, simple parameter settings and fast convergence. First these problems are trans- formed into ones with continuous variables by defining an equivalent probability matrix in this paper, then multi-objective particle swarm optimization based on the minimal particle angle is used to solve them. Methods such as continuation of discrete variables, update of particles for matrix variables, normalization of particle position and evalua- tion of particle fitness are presented. Finally the efficiency of the proposed method is validated by comparing it with other methods on an eight-project-ten-site location problem.

关键词： multi-project location problems multi-objective optimization particle swarm optimization

Updating magnetic resonance imaging of small bowel:Imaging protocols and clinical indications

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World Journal of Gastroenterology 2008年第21期14卷 3403-3409页

作者： Jiong Zhu Jian-Rong Xu Hong-Xia gong Yan zhou Department of Radiology Ren Ji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127 China

High soft tissue contrast resolution,acquisition of multi-planar images and the possibility to obtain functional information make magnetic resonance an interesting imaging technique to evaluate the small bowel *** absence of ionizing radiation is an important feature of magnetic resonance imaging(MRI) examinations because inflammatory diseases such as Crohn's disease(CD) are studied most frequently,which are prevalent among children and young ***,using modern equipment and a rigorous technical approach,can offer detailed morphologic information and functional data on the small *** article discusses the MRI protocols for small bowel and the MR imaging findings of small bowel diseases,such as CD and small bowel neoplasms.

关键词： Magnetic resonance imaging Small bowel Crohn's Neoplasm

Deciphering In-vivo Cross-linking Mass Spectrometry Data for Dynamic Protein Structure Analysis

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Chemical Research in Chinese Universities 2022年第3期38卷 758-762页

作者： ZHAO Lili gong zhou ZHAO Qun ZHANG Lihua ZHANG Yukui CAS Key Laboratory of Separation Science for Analytical Chemistry National Chromatographic R.&A.CenterDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023P.R.China University of Chinese Academy of Sciences Beijing100039P.R.China Innovation Academy for Precision Measurement Science and Technology Chinese Academy of SciencesWuhan430071P.R.China

Protein structure and protein-protein interactions(PPIs)are crucial for regulating cellular activities required for cell viability and *** cross-linking coupled with mass spectrometry(CXMS)has become a versatile tool providing insights into both protein structure with distance restraints and protein-protein interactions with interface ***-links as the most information-rich data in a CXMS experiment are responsible for the structural model validation and integrative modeling with high throughput and *** this work,ensemble refinement of the existing protein structure against the in-vivo cross-linking distance restraints was performed for dynamic protein structure modeling and protein interaction binding interface building in the intracellular *** results indicate great potential of in-vivo CXMS data for providing a molecular basis of protein structural dynamics exploration and function performance.

关键词： In-vivo chemical cross-linking Cross-linking distance restraint Ensemble refinement Structural dynamics Disorder region

Effect of cement on the stabilization of loess

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Journal of Mountain Science 2017年第11期14卷 2325-2336页

作者： ZHANG Chong-lei JIANG Guan-lu SU Li-jun zhou gong-dan Key Laboratory of Mountain Hazards and Earth Surface Processes Institute of Mountain Hazards and Environment Chinese Academy of Sciences Key Laboratory of High-speed Railway Engineering of Ministry of Education School of Civil Engineering Southwest Jiaotong University CAS Center for Excellence in Tibetan Plateau Earth Sciences University of Chinese Academy of Sciences

Considering the potential use of cementstabilized loess(CSL) as a construction material for structures that are subjected to frequent loess landslides, this paper explores the stabilization and improvement of geotechnical characteristics of loess achieved by the addition of 0%-9% cement by dry weight. Laboratory evaluations investigated the consistency limits, compaction, compressibility, California bearing ratio(CBR), direct shear strength, and unconfined compression strength(UCS) of CSL for different curing stages. A durability index was quantified to estimate the influence of wetting-drying(w-d) cycles on CSL strength, and an optimum cement dosage was also identified. The results reveal that the cohesion of CSL is substantially more sensitive to structure than its friction angle and that cohesion is responsible for shear strength increase after remoulding. The cement proportions have an effective role in the enhancement of compressibility. The development of UCS can be categorized into the early stage(14 days). The increase in strength primarily occurred in the first 14 days. The w-d cycles have a significantinfluence on the decrease in compression strength. The CBR value increases with increments of additional proportions and compaction times. The relationships of UCS versus the compressibility modulus and UCS versus CBR are established to facilitate the mix design for strength. A rational predictive exponential equation is proposed to predict the durability index for different w-d cycles.

关键词： Landslide Cement Loess stabilization Durability index Wetting-drying cycle

Effect of shenfu injection on gastrointestinal microcirculation in rabbits after myocardial ischemia-reperfusion injury

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World Journal of Gastroenterology 2006年第27期12卷 4389-4391页

作者： Xue-Juan Zhang Li Song Zan-gong zhou Xiu-Mei Wang Department of Cardiology Affiliated Hospital of Qingdao Medical University Qingdao 266003 Shandong Province China

AIM： To investigate the effect of shenfu injection on gastrointestinal microcirculation after myocardial ischemic-reperfusion （IR） injury in rabbits and probe into the mechanism. METHODS： Forty healthy flap-eared white rabbits were randomly divided into 4 groups： IR injury control group （group Ⅰ ）, shenfu injection 5 mL/kg per h group （group Ⅱ）, shenfu injection 10 mL/kg per h group （group Ⅲ） and shenfu injection 20 mL/kg per h group （group Ⅳ）. The four groups were treated with Lactated Ringer＇s solution, shenfu injection 5, 10, and 20 mL/ kg per h were infused intravenously 30 min before experiment respectively. The values of hemodynamics [mean arterial pressure （MAP）, heart rate （HR）, gastric intramucosal partial pressure of carbon dioxide （PCO2）, blood gas analysis and pH] were measured and compared with those before myocardial ischemia, 60 min after myocardial ischemia and 60, 90, and 180 rain after reperfusion. RESULTS： The MAP, HR and gastric intramucosal pH were （70.50 ± 4.50） kPa, （165 ± 14） beats per rain, 7.032 ± 0.024 in group Ⅰ 60 min after myocardial ischemia, which were significantly decreased compared with those before myocardial ischemia （88.50 ± 9.75 kPa, 217 ± 18 beats per rain, 7.112 ± 0.035, P 〈 0.05）. The MAP, HR and gastric intramucosal pH were significantly decreased in group Ⅰ 60, 90, and 180 min after reperfusion （61.50 ± 5.25 kPa, 133 ± 31 beats per rain, 6.997 ± 0.025） compared with those before reperfusion respectively （P 〈 0.05）, whereas the values were insignificantly different in groups Ⅱ, Ⅲ or Ⅳ after reperfusion, compared with those before reperfusion, and there were no significant differences between groups Ⅱ, Ⅲ, and Ⅳ after reperfusion. CONCLUSION： Pre-infusion of shenfu injection has a protective effect on gastrointestinal microcirculation after myocardial IR injury in rabbits, in a dose independent manner.

关键词： Shenfu injection Myocardial ischemicreperfusion injury Gastrointestinal microcirculation

Extending the Bandwidth of Electric Ring Resonator Metamaterial Absorber

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Chinese Physics Letters 2011年第3期28卷 85-88页

作者： LUO Hao WANG Tao gong Rong-zhou NIE Yan WANG Xian Department of Electronic Science and Technology Huazhong University of Science and TechnologyWuhan 430074 College of Physics and Electronic Engineering Xinyang Normal UniversityXinyang 464000

An efficient method is proposed to extend the bandwidth of a metamaterial absorber with multi-resonance *** basic unit cell of a metamaterial absorber consists of the electric ring resonator,dielectric substrate(FR-4)and *** assembling five sandwiched structures with different geometric dimensions into a unit cell,we obtain the superposition of five different absorption *** the bandwidth of metamaterial absorption is extended and the full width at half maximum is up to 1.3 *** simulated and experimental results are consistent.

关键词： structure absorber sandwich

Comparison of Rheometric Devices for Measuring the Rheological Parameters of Debris Flow Slurry

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Journal of Mountain Science 2015年第5期12卷 1125-1134页

作者： YANG Hong-juan WEI Fang-qiang HU Kai-heng zhou gong-dan LYU Juan Key Laboratory of Mountain Hazards and Earth Surface Process Chinese Academy of Sciences Institute of Mountain Hazards and Environment Chinese Academy of Sciences

Soil samples with clay content ranging from 15% to 31%, were taken from three debris flow gullies in Southwest China. Three debris flow slurry samples were prepared and tested with four measuring systems of an Anton Paar Physica MCR301 rheometer, including the concentric cylinder system,the parallel-plate system, the vane geometry, and the ball measuring system. All systems were smoothwalled. Flow curves were plotted and yield stress was determined using the Herschel-Bulkley model,showing differences among the different *** curves from the concentric cylinder and parallelplate systems involved two distinct regions, the low shear and the high shear regions. Yield stresses determined by data fitting in the low shear region were significantly lower than the values from the inclined channel test which is a practical method for determining yield stress. Flow curves in the high shear region are close to those from the vane geometry and the ball measuring system. The fitted values of yield stress are comparable to the values from the inclined channel test. The differences are caused by wall-slip effects in the low shear *** geometry can capture the stress overshoot phenomenon caused by the destruction of slurry structure, whereas end effects should be considered in the determination of yield stress. The ball measuring system can give reasonable results, and it is applicable for rheological testing of debris flow slurries.

关键词： Debris flow Slurry Rheometer Concentric cylinder system Parallel plate system Vane Ball measuring system

Effect of dry density on the liquefaction behaviour of Quaternary silt

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Journal of Mountain Science 2018年第7期15卷 1597-1614页

作者： ZHANG Chong-lei JIANG Guan-lu SU Li-jun Liu Wei-ming zhou gong-dan Key Laboratory of Mountain Hazards and Earth Surface Processes Institute of Mountain Hazards and EnvironmentChinese Academy of Sciences Chengdu 610043 China Ministry of Education Key Laboratory of High-speed Railway Engineering School of Civil Engineering Southwest Jiaotong University Chengdu 610031 China CAS Center for Excellence in Tibetan Plateau Earth Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

Quaternary silt is widely distributed in China and easily liquefies during earthquakes. To identify the influence of the dry density on the liquefaction behaviour of Quaternary silt, 40 cyclic triaxial liquefaction tests were performed on loose silt(dry density rd=1.460 g/cm^3) and dense silt(rd=1.586 g/cm^3) under different cyclic stress ratios(CSRs) to obtain liquefaction assessment criteria, determine the liquefaction resistance, improve the excess pore water pressure(EPWP) growth model and clarify the relationship between the shear modulus and damping ratio. The results indicate that the initial liquefaction assessment criteria for the loose and dense silts are a double-amplitude axial strain of 5% and an EPWP ratio of 1. The increase in the anti-liquefaction ability for the dense silt is more significant under lower confining pressures. The CSR of loose silt falls well within the results of the sandy silt and Fraser River silt, and the dense silt exhibits a higher liquefaction resistance than the sand-silt mixture. The relationships between the CSR and loading cycles were obtained at a failure strain of 1%. The EPWP development in the dense and loose silts complies with the "fast-stable" and "fast-gentle-sharp" growth modes, respectively. The power function model can effectively describe the EPWP growth characteristics of the dense silt. Finally, based on the liquefaction behaviour of silt, a suggestion for reinforcing silt slopes or foundations is proposed.

关键词： Liquefaction Quaternary silt Dry density Earthquake magnitude Liquefaction assessment Cyclic stress ratio