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Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization

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Chinese Science Bulletin 2008年第19期53卷 2973-2980页

作者： YI ChuanDeng TANG ShuZhu ZHOU Yong LIANG GuoHua gong zhiyun GU MingHong Jiangsu Province Key Laboraty of Crop Genetics and Physiology/Key Laboraty of Plant Functional Genomics of Ministry of Education Yangzhou University Yangzhou 225009 China

Oryza sativa and O. latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, interspecific hybrids of these species were obtained using the embryo rescue technique. Hybrid panicle traits, such as long awns, small grain, exoteric large purple stigma, grain shattering and dispersed panicles, resemble that of the paternal parent, O. latifolia, whereas there is obvious heterosis in such respects as plant height, tillering ability and vegetative vigor. Chromosome pairing and the genomic components of the hybrid were subsequently investigated using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) analysis. Based on the mitotic metaphase chromosome numbers of the root tips investigated, the hybrid is a triploid with 36 chromosomes. The genomic constitution of the hybrid is ACD. In the meiotic metaphase I of the hybrid pollen mother cell, poor chro- mosome pairing was identified and most of the chromosomes were univalent, which resulted in com- plete male sterility in the hybrid.

关键词：漂白亚麻纤维卷胚芽种间杂交技术

Generating of rice OsCENH3-GFP transgenic plants and their genetic applications

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Chinese Science Bulletin 2008年第19期53卷 2981-2988页

作者： YU HengXiu WANG Xin gong zhiyun TANG Ding GU MingHong CHENG ZhuKuan Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education Yangzhou University Yangzhou 225009 China Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

In order to investigate rice functional centromeres, OsCENH3-GFP chimeric gene was constructed and transformed into the indica rice variety, Zhongxian 3037, mediated by Agrobacturium. The integration of the exogenous genes in the transgenic plants was confirmed by PCR and Southern blotting. The transgenic plants grow normally during their whole life time, just like Zhongxian 3037. No significant defects were detected in either mitosis or meiosis of the transgenic plants. The overlapping of GFP signals and anti-CENH3 foci in both mitotic and meiotic cells from T0 and T1 generation plants indicated that GFP had been successfully fused with CENH3, so the GFP signals can well represent the CENH3 locations on each chromosome. To evaluate the applicability of the transgenic plants to other genetic studies, fluorescence in situ hybridization (FISH) using rice centromeric tandem repetitive sequence CentO as the probe was conducted on the zygotene chromosomes of pollen mother cells (PMCs). It has been revealed that the GFP signals are overlapping with CentO FISH signals, showing that CentO is one of the key elements constituting rice functional centromeres. Immunofluorescent staining using anti-α-tublin antibody and anti-PAIR2 antibody on the chromosomes during mitosis and meiosis stages of the transgenic plants further reveals that OsCENH3-GFP transgenic plants can be widely used for studying rice molecular biology, especially for tagging functional centromeres in both living cells and tissues.

关键词：着丝点稻子遗传物质栽培技术

Isolation and Identification of a Functional Centromere Element in the Wild Rice Species Oryza granulata with the GG Genome

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Journal of Genetics and Genomics 2015年第12期42卷 699-702页

作者： Chuandeng Yi Mingsen Wang Wei Jiang Derong Wang Yong Zhou zhiyun gong Guohua Liang Minghong Gu Jiangsu Key Laboratory of Crop Genetics and Physiology Co-Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou University

The centromere of eukaryotic chromosomes is the crucial locus responsible for sister chromatid cohesion and for correct segregation of chromosomes to daughter cells during cell division. In the structural genomics era, centromeres represent the last frontiers of higher eukaryotic genomes because of their densely methylated, highly repetitive and, heterochromatic DNA （Hall et al., 2004）. Although these functions are conserved among all eukaryotes, centromeric DNA sequences are evolving rapidly （Jiang et al., 2003）.

关键词： FISH BAC Isolation and Identification of a Functional Centromere Element in the Wild Rice Species Oryza granulata with the GG Genome

Histone deacetylase OsHDA706 increases salt tolerance via H4K5/K8 deacetylation of OsPP2C49 in rice

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Journal of Integrative Plant Biology 2023年第6期65卷 1394-1407页

作者： Kai Liu Jijin Chen Shang Sun Xu Chen Xinru Zhao Yingying Hu Guoxiao Qi Xiya Li Bo Xu Jun Miao Chao Xue Yong Zhou zhiyun gong Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology Agricultural College of Yangzhou UniversityYangzhou 225009China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009China Institute of Lianyungang Agricultural Science of Xuhuai Area Lianyungang 222006China

High salt is a major environmental factor that threatens plant growth and *** evidence indicates that histone acetylation is involved in plant responses to various abiotic stress;however,the underlying epigenetic regulatory mechanisms remain poorly *** this study,we revealed that the histone deacetylase OsHDA706 epigenetically regulates the expression of salt stress response genes in rice(Oryza sativa L.).OsHDA706 localizes to the nucleus and cytoplasm and OsHDA706 expression is significantly induced under salt ***,oshda706 mutants showed a higher sensitivity to salt stress than the *** vivo and in vitro enzymatic activity assays demonstrated that OsHDA706 specifically regulates the deacetylation of lysines 5 and 8 on histone H4(H4K5and H4K8).By combining chromatin immunoprecipitation and mRNA sequencing,we identified the clade A protein phosphatase 2C gene,OsPP2C49,which is involved in the salt response as a direct target of H4K5 and H4K8 *** found that the expression of OsPP2C49 is induced in the oshda706 mutant under salt ***,the knockout of OsPP2C49 enhances plant tolerance to salt stress,while its overexpression has the opposite *** together,our results indicate that OsHDA706,a histone H4 deacetylase,participates in the salt stress response by regulating the expression of OsPP2C49 via H4K5 and H4K8 deacetylation.

关键词： histone deacetylase OsHDA706 OsPP2C49 rice salt stress

Centromere inactivation in a dicentric rice chromosome during sexual reproduction

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Chinese Science Bulletin 2013年第33期58卷 4602-4607页

作者： gong zhiyun XUE Chao LIU XiuXiu ZHANG MingLiang ZHOU Yong YU HengXiu GU MingHong Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education Yangzhou University

T0135 is a variant selected from the progeny of a rice line telotrisomic for the short arm of chromosome 11(2n+11S·).Fluorescent in situ hybridization(FISH)results indicated that T0135 contained two telocentric chromosomes,which have two centromere-specific molecular markers(5S rDNA)for chromosome 11;thus T0135 is a newly-described rice chromosome variant with two dicentric chromosomes,named 22+11L·+11L·+11S·11S·+11S·11S·(22 represents the 22 chromosomes excluding chromosome 11 in the rice genome,"·"represents the centromere).To investigate the genetic stability of the rice dicentric chromosomes during sexual reproduction,we observed the chromosome types in the ***-four percent of the progeny had the same chromosome type as the parental *** result indicates that the dicentric chromosomes are mostly stable during mitosis and *** analysis for centromere specific histone H3(CENH3)revealed that only one centromere is active and the other centromere is inactivated in the rice dicentric chromosomes.

关键词： 1号染色体着丝粒繁殖过程遗传稳定性水稻品系失活免疫荧光分析分子标志物

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Diagnosis and Monitoring Value of Circulating Tumour Cells in Breast Cancer with an Optimised Microfluidic Device:A Retrospective Study

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Diagnosis and Monitoring Value of Circulating Tumour Cells i...

Histone Acetyltransferase GCN5 Regulates Rice Growth and Development and Enhances Salt Tolerance

2022CCTB中国肿瘤标志物学术大会暨中国整合肿瘤学大会暨第十六届肿瘤标志物青年科学家论坛暨中国肿瘤标志物产业创新大会

作者： zhiyun gong Renquan Lu Department of Clinical Laboratory Fudan University Shanghai Cancer Center

Background and aims:More excellent detection system of circulating tumor cells(CTCs) is *** we performed CTCs detection with a novel automatic *** and methods:137 breast cancer patients,49 patients with benign breast diseases and42 healthy volunteers were *** blood was obtained and followed CTCs *** performance was verified by spiking assay and clinical *** number of CTCs and CD45+ CTCs were analyzed along with clinicopathologic features.A short-term follow-up review was presented for treatment monitoring by CTC and CD45+ ***:In general,CTCs were found in 86.86% of patients with malignant breast cancer and63.27% of patients with benign breast diseases but only 1 in healthy *** sensitivity and specificity of 74.5% and 90.1% respectively when the cut-off was *** significant differences of CTC level were found between malignant and non-malignant group(P<0.01),tumor progression(P= 0.0216) and tumour size(P= 0.0326).In follow-up,the 65.22% of patients exhibited reduction in CTCs count because ***,dual-positive CTC(CD45+/CK+) exerted descending pattern during treatment period in patients undergone chemotherapy,target-therapy,hormonotherapy and ***:A qualified performance of this novel platform was validated in our *** and CD45+ CTCs both have clinical significance in breast cancer diagnosis and monitoring.

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Rice science 2024年第6期31卷 688-699,I0015页

作者： XUE Chao ZHAO Xinru CHEN Xu CAI Xingjing HU Yingying LI Xiya ZHOU Yong gong zhiyun Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Agricultural College Yangzhou UniversityYangzhou 225009China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou UniversityYangzhou 225009China School of Agronomy and Horticulture Jiangsu Vocational College of Agriculture and ForestryZhenjiang 212499China

Histone acetylation is indispensable in the process of crops resisting abiotic stress,which is jointly catalyzed by histone acetyltransferases and ***,the mechanism of regulating salt tolerance through histone acetyltransferase GCN5 is still *** revealed that GCN5 can catalyze the acetylation of canonical H3 and H4 lysine residues both in vivo and in vitro in *** knockout mutants and RNA interference lines of Os GCN5 exhibited severe growth inhibition and defects in salt tolerance,while the over-expression of Os GCN5 enhanced the salt tolerance of rice seedlings,indicating that Os GCN5 positively regulated the response of rice to salt ***-seq analysis suggested Os GCN5 may positively regulate the salt tolerance of rice by inhibiting the expression of Os HKT2;1 or other salt-responsive *** together,our study indicated that GCN5 plays a key role in enhancing salt tolerance in rice.

关键词： GCN5 histone acetyltransferase salt tolerance Oryza sativa

隐秘而矛盾的象征——高更艺术中的“人”与“相”

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艺术设计研究 2022年第1期 103-109页

作者：龚之允上海师范大学美术学院

高更艺术的魅力,既源于艺术家离经叛道的艺术实践,也与其矛盾丛生的造型语言息息相关。高更自认为是一个远离欧洲现代文明的纯粹者,另一面却一直制造着一种与现实状况并不相符的个人形象,以达到引领艺术方向的目的,以实现其艺术大师的... 详细信息

高更艺术的魅力,既源于艺术家离经叛道的艺术实践,也与其矛盾丛生的造型语言息息相关。高更自认为是一个远离欧洲现代文明的纯粹者,另一面却一直制造着一种与现实状况并不相符的个人形象,以达到引领艺术方向的目的,以实现其艺术大师的理想。本文将围绕文明衍进、前卫趋向和自我认知这三个方面的矛盾,讨论高更对"人"与"相"的认知和表达,希望能够解答其艺术理念中隐秘象征的内核。

关键词：高更象征主义他者矛盾

Mapping of QTLs ControllingTissue Culture Response in Rice

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Mapping of QTLs ControllingTissue Culture Response in Rice