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Effects of Shot Peening Process on Thermal Cycling Lifetime of TBCs Prepared by EB-PVD

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Chinese Journal of Aeronautics 2007年第2期20卷 145-147页

作者： ZHOU Zhao-hui gong sheng-kai LI He-fei XU Hui-bin ZHANG Chun-gang WANG Lu School of Materials Science and Engineering Beij＇ing University of Aeronautics and Astronautics Beij＇ing 100083 China Shenyang Liming.4ero-Engine Group Corporation Shenyang 110043 China

Conventional two-layered thermal barrier coatings （TBCs） are prepared by electron beam physical vapor deposition （EB-PVD） with ZrO2-8 wt% Y2O3 （8YSZ） as top coat and CoCrAlY as bond coat on disk-shaped Ni based super-alloy. In this paper, three kinds of shot peening process with different lengths of operating time were adopted for bond coating. As a result, changes took place in its surface roughness and the surface micro-hardness. A thermal cycling test at 1 273 Kx55 rain and another at room temperature for 5 min were performed to study the effects of shot peening process on the thermal cycling lifetime of TBCs. It is found that a moderate shot peening process will be able to prolong the life time. The oxidation dynamic of the as-processed TBCs basically accords with the parabolic rule, and the oxidation test also attests to the spallation between YSZ and thermal growth oxide （TGO） responsible mainly for the failure of TBCs.

关键词： thermal cycling lifetime shot peening thermal barrier coatings (TBCs)

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中洞法施工技术

岩土工程学报 2006年第B11期28卷 1772-1775页

作者：龚胜广东省基础工程公司广东广州510620

中洞法作为矿山法的一种,具有如下特点:安全性高,灵活性好,可操作性强,机械化程度低,出土效率高,工序间干扰较少等。北京地铁四号线石榴庄隧道工程中采用了中洞法施工技术,工程将中洞法施工划分成13个步骤,遵照“小分块、短台阶、早成... 详细信息

中洞法作为矿山法的一种,具有如下特点:安全性高,灵活性好,可操作性强,机械化程度低,出土效率高,工序间干扰较少等。北京地铁四号线石榴庄隧道工程中采用了中洞法施工技术,工程将中洞法施工划分成13个步骤,遵照“小分块、短台阶、早成环、环套环”的原则,先进行中洞施工,然后两侧洞同步采用正台阶法自上而下分部掘进成环施工,有效地控制了地面沉降,保证了施工安全。

关键词：中洞法地铁隧道施工技术

中国近海新生代盆地至今仍然是油气成藏的活跃期

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石油学报 2005年第6期26卷 1-6页

作者：龚再升中国海洋石油总公司北京100027

传统的石油地质学理论表明:石油、天然气是在漫长的地质历史时间内由有机质降解产生的,又是在漫长的地质历史时间内,缓慢渗流运移到有圈闭的储集层中。在中国近海油气勘探实践中,运用了这些传统的理论和概念,又逐步充实了某些新的认识,... 详细信息

传统的石油地质学理论表明:石油、天然气是在漫长的地质历史时间内由有机质降解产生的,又是在漫长的地质历史时间内,缓慢渗流运移到有圈闭的储集层中。在中国近海油气勘探实践中,运用了这些传统的理论和概念,又逐步充实了某些新的认识,即油气生成、运移、聚集不只是漫长地质历史时期中存在的,而且至今仍然存在;特别是上新世至今的新构造运动对盆地的油气田分布和定型都有重要的控制作用。新构造运动控制晚期油气成藏,而且总是处于供聚大于逸散的动平衡状态中。以墨西哥湾尤金岛330区块油气晚期补给、渤海盆地PL19-3油田、莺歌海DF1-1气田晚期成藏以及我国近期几次大地震与油气相关性来阐述至今油气仍处于活跃的成藏过程及可能存在的突发性的幕式快速运聚充注过程,其中天然地震可能是油气运移的主要驱动力之一。

关键词：新构造运动晚期成藏天然地震驱动力新生代中国近海盆地

Gene transfer into primary cultures of fetal neural stem cells by a recombinant adenovirus carrying the gene for green fluorescent protein

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第4期9卷 299-305页

作者： Yong FU Shen-qing WANG Ying-peng LIU Guo-peng WANG Jian-ting WANG Shu-sheng gong Department of Otorhinolaryngology the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China Department of Otorhinolaryngology Union Hospital of Tongii Medical College Huazhong University of Science and Technology Wuhan 430022 China Department of Surgery-Otolaryngology University of Kentucky Medical Center Lexington Kentucky 40536 USA Department of Surgery-Otolaryngology Beijing Tongren Hospital Capital Medical University Beijing 100730 China

Objective: To evaluate the transduction efficiency of a recombinant adenovirus carrying the gene for green fluorescent protein (Ad-GFP) into the primary cultures of fetal neural stem cells (NSCs) by the expression of GFP. Methods: The Ad-GFP was constructed by homologous recombination in bacteria with the AdEasy system; NSCs were isolated from rat fetal hippocampus and cultured as neurosphere suspensions. After infection with the recombinant Ad-GFP, NSCs were examined with a fluorescent microscopy and a flow cytometry for their expression of GFP. Results: After the viral infection, flow cytometry analysis revealed that the percentage of GFP-positive cells was as high as 97.05%. The infected NSCs sustained the GFP expression for above 4 weeks. After differentiated into astrocytes or neurons, they continued to express GFP efficiently. Conclusion: We have success- fully constructed a viral vector Ad-GFP that can efficiently infect the primary NSCs. The reporter gene was showed fully and sustained expression in the infected cells as well as their differentiated progenies.

关键词： Recombinant adenovirus vector Viral infection Fetal neural stem cells Green fluorescent protein

Co-doped IT-M0S2 nanosheets embedded in N,S-doped carbon nanobowls for high-rate and ultra-stable sodium-ion batteries

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Nano Research 2019年第9期12卷 2218-2223页

作者： Peihao Li Yong Yang sheng gong Fan Lv Wei Wang Yiju Li Mingchuan Luo Yi Xing Qian Wang Shaojun Guo Department of Materials Science & Engineering College of EngineeringPeking UniversityBeijing 100871China Department of Materials Science and Engineering Massachusetts Institute of TechnologyCambridgeMA 02139USA Center far Applied Physics and Technology College of EngineeringPeking UniversityBeijing 100871China BIC-ESAT College of EngineeringPeking UniversityBeijing 100871China

Despite various 2H-MoS/carbon hybrid nanostructures have been constructed and committed to improve the performance for sodium-ion batteries(SIBs),they still show the limited cycle stability due to the relatively large volumetric expansion during the charge-discharge process Herein,we report the construction of cobalt-doped few-layered 1T-MoS2 nanosheets embedded in N,S-doped carbon(CMS/NSC)nanobowls derived from metal-organic framework(MOF)precursor via a simple in situ sulfurization *** unique hierarchical structure enables the uniformly dispersed Co-doped 1T-MoS2 nanosheets intimately couple with the highly conductive carbon nanobowls,thus efficiently preventing the *** particular,the Co-doping plays a crucial role in maintaining the integrity of structure for MoS2 during cycling tests,confirmed by first-principles *** with pristine MoS2,the volume deformation of Co-doped MoS2 can be shrunk by a prominent value of 52%during ***,the few-layered MoS2 nanosheets with 1T metalic phase endow higher conductivity,and thus can surpass its counterpart 2H semiconducting phase in battery *** virtue of the synergistic effect of stable structure,appropriate doping and high conductivity,the resulting CMS/NSC hybrid shows superior rate capability and cycle *** capacity of CMS/NSC can still be 235.9 mAh·g^-1 even at 25 A·g^-1,which is 51.3%of the capacity at 0.2 A·g^-***,the capacity can still remain 218.6 mAh·g^-1 even over 8,240 cycles at 5 Ag·g^-1 with a low decay of 0.0044%per cycle,one of the best performances among the reportec MoS2-based anode materials for SIBs.

关键词： sodium-ion battery anode IT-MoS2 cobalt-doping metal-organic framework(MOF)

Atomistic simulation of the coupled adsorption and unfolding of protein GB1 on the polystyrenes nanoparticle surface

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Science China(Physics,Mechanics & Astronomy) 2018年第3期61卷 56-64页

作者： HuiFang Xiao Bin Huang Ge Yao WenBin Kang sheng gong Hai Pan Yi Cao Jun Wang Jian Zhang Wei Wang School of Physics Collaborative Innovation Center of Advanced Microstructures and National Laboratory of Solid State Microstructures Nanjing University Nanjing 210093 China Department of Pharmaceutics Jinling Hospital Nanjing University School of Medicine Nanjing 210002 China School of Public Health and Management Hubei University of Medicine Shiyan 442000 China

Understanding the processes of protein adsorption/desorption on nanopartieles＇ surfaces is important for the development of new nanotechnology involving biomaterials; however, an atomistic resolution picture for these processes and for the simultaneous protein conformational change is missing. Here, we report the adsorption of protein GB 1 on a polystyrene nanoparticle surface using atomistic molecular dynamic simulations. Enabled by metadynamics, we explored the relevant phase space and identified three protein states, each involving both the adsorbed and desorbed modes. We also studied the change of the secondary and tertiary structures of GB 1 during adsorption and the dominant interactions between the protein and surface in different adsorption stages. The results we obtained from simulation were found to be more adequate and complete than the previous one. We believe the model presented in this paper, in comparison with the previous ones, is a better theoretical model to understand and explain the experimental results.

关键词： protein adsorption atomistic simulations free energy landscape biomaterials nanomaterials metadynamics

Synthesis,Structure and Optical Properties of Cerium(Ⅲ) Triphosphate CeP_3O_9

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Chinese Journal of Structural Chemistry 2007年第3期26卷 338-346页

作者： TONG Hua-Nan ZHANG Hao CHENG Wen-Dan WU Dong-sheng gong Ya-Jing ZHU Jing HUANG Shu-Ping ZHAO Dan State Key Laboratory of Structural Chemistry Fujian Institute of Research on theStructure of Matter The Chinese Academy of Sciences Fuzhou Fujian 350002 China

The single crystal and crystallized powder of triphosphate CeP3O9 have been synthesized, and the space group of CeP3O9has been determined to be C2221 with the cell parameters ofa = 8.6059, b = 11.2437, c = 7.3518 A, V= 711.4（3） A^3, Z= 4, D,. = 3.520 g/cm^3, F（000） = 700, R = 0.0377 and wR = 0.0930. The absorption and emission spectra have been measured, for which the strongest absorption and emission peaks are located at 280 and 320 nm, respectively. The density of state （DOS） and dielectric function have been calculated by the DFT method. The crystal is transparent provided the wavelength is larger than 341 nm, and the observed ultraviolet cut-off edge is at about 350 nm for a polycrystalline power sample. It is possible that the triphosphate CeP3O9 will become an ultraviolet emission material.

关键词： crystal structure phosphate optical properties density of states

Effects of siRNA specific to the protein kinase CK2α on apoptosis of laryngeal carcinoma cells

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Chinese Medical Journal 2012年第9期125卷 1581-1585页

作者： WANG Jian-ting gong Shu-sheng Department of Otorhinolaryngology Head and Neck Surgery Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China Department of Otorhinolaryngology Head and Neck Surgery Beijing Tongren Hospital Capital Medical University Beijing 100730 China

Background The relationship between apoptosis and tumors is a major focus in cancer research. RNA interference （RNAi） technology has emerged as a very potent tool to generate cellular knockdown phenotypes of a desired gene. The aim of this study was to explore the effect of siRNA specific to the protein casein kinase 2α （CK2α） on apoptosis of laryngeal carcinoma cells and to explore possible mechanisms.Methods An siRNA expression plasmid specific to CK2α, psiRNA-hH1neo-CK2α, and a non-specific siRNA expression plasmid, psiRNA-hH1neo-cont, were constructed and transfected into Hep-2 cells by a lipofectamine method. The mRNA and protein levels of CK2α in transfected cells were determined by reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting analysis. Apoptosis was measured by Annexin V-fluorescein isothiocyanate （FITC）/ propidium iodide （PI） double-staining methods. The morphological changes to Hep-2 cells were observed under transmission electron microscopy （TEM）. The levels of Bcl-2 and Bax proteins were measured by Western blotting analysis.Results Levels of CK2α mRNA and protein were significantly decreased in the psiRNA-hH1neo-CK2α group compared to the other groups （P 〈0.05）. The apoptotic rate of the psiRNA-hH1neo-CK2α transfected group was significantly higher compared to that in the untransfected group and the siRNA-hH1neo-cont transfected group （25.66%±0.83%, 3.66%±0.43%, and 5.18%±0.22%）（P 〈0.05）. Compared with the untransfected group and the siRNA-hH1neo-cont transfected group, the psiRNA-hH1neo-CK2α transfected group presented with classical ultrastructural features of apoptosis, such as karyopyknosis, chromatic agglutination adjacent to the nuclear membrane, and apoptotic bodies. Compared with the other two groups, the level of Bcl-2 protein in the psiRNA-hH1neo-CK2α transfected group was decreased （0.20±0.09 vs. 0.72±0.16, 0.56±0.11, P 〈0.01）, while the Bax protein level was increased （0.81±0.17 vs. 0.26±0.12, 0.33±0.1

关键词： RNA interference small interfering RNA laryngeal neoplasms apoptosis casein kinase 2

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我国黄磷深加工发展方向的探讨

现代化工 2006年第5期26卷 1-6页

作者：贡长生武汉化工学院化工与制药学院湖北武汉430073

根据我国黄磷工业快速发展的实际情况,研究和探讨了黄磷深加工产品的发展方向。重点论述了黄磷深加工和精细化的发展方向,介绍了黄磷直接催化合成亚磷酸和有机磷化合物的最新研究进展。在分析国内黄磷深加工发展趋势的基础上,对我国黄... 详细信息

根据我国黄磷工业快速发展的实际情况,研究和探讨了黄磷深加工产品的发展方向。重点论述了黄磷深加工和精细化的发展方向,介绍了黄磷直接催化合成亚磷酸和有机磷化合物的最新研究进展。在分析国内黄磷深加工发展趋势的基础上,对我国黄磷深加工的发展问题提出了建议。

关键词：黄磷深加工精细磷酸盐有机磷化合物