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R-(+)-长叶薄荷酮对腹腔感染耐药菌小鼠的抗炎作用

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中华医院感染学杂志 2024年第16期34卷 2405-2410页

作者：宫海燕杨珊珊王晓娟郑琳川季兴磊熊文娟广东医科大学附属东莞第一医院检验科广东东莞523710 新疆医科大学第五附属医院(第五临床医学院)检验科新疆乌鲁木齐830011

目的明确腹腔感染耐药菌小鼠的半数致死浓度(LC50)和绝对致死浓度(LC100),探讨R-(+)-长叶薄荷酮对腹腔感染耐药菌小鼠的抗炎作用。方法随机将小鼠分为正常对照组(NC组)、耐药菌株感染模型组(Model组)、R-(+)-长叶薄荷酮组(PU组,20 mg/... 详细信息

目的明确腹腔感染耐药菌小鼠的半数致死浓度(LC50)和绝对致死浓度(LC100),探讨R-(+)-长叶薄荷酮对腹腔感染耐药菌小鼠的抗炎作用。方法随机将小鼠分为正常对照组(NC组)、耐药菌株感染模型组(Model组)、R-(+)-长叶薄荷酮组(PU组,20 mg/kg)、头孢克肟组(CFM组,26 mg/kg),每组10只;除NC组外,其他试验组进行干预,连续3 d, 1次/d, Model组按照20 ml/kg经口灌胃食用油,PU组、CFM组分别给予等体积的R-(+)-长叶薄荷酮、头孢克肟,第3天给药后1 h,腹腔注射耐药大肠埃希菌(10 ml/kg),观察24、48 h后收集小鼠眼眶血、腹腔冲洗液、腹膜组织;采用全自动细胞分析仪进行血液、腹腔冲洗液的白细胞计数;瑞氏-吉姆萨染色观察血细胞形态变化;苏木精-伊红(HE)染色法观察腹腔组织病理形态变化。结果腹腔感染耐药菌小鼠的LC50和LC100分别是1.50×10~8 CFU/ml、2.25×10~8 CFU/ml;与NC组相比,Model组小鼠腹腔内存在弥漫性炎症,腹腔充血、水肿;而PU组、CFM组小鼠腹腔内炎症、充血、水肿程度较Model组不明显;眼眶血白细胞计数表明PU组较NC组有所降低,较Model组、CFM组无统计学差异;腹腔冲洗液白细胞计数PU组低于Model组,较NC组增高,较CFM组无统计学差异,表明R-(+)-长叶薄荷酮对腹腔感染耐药菌小鼠具有抗炎作用(P<0.05);HE结果显示,PU组、CFM组腹膜组织结构完整,间皮细胞排列整齐,腹膜组织间质较NC组可见少量淋巴细胞浸润,相对于Model组腹膜组织结构更加完整,淋巴细胞浸润较少,表明R-(+)-长叶薄荷酮对腹腔耐药菌的感染具有显著的抗感染保护作用。结论 R-(+)-长叶薄荷酮对腹腔感染耐药菌小鼠具有一定抗炎作用,为R-(+)-长叶薄荷酮抗感染药理作用机制的探索提供一定的研究基础。

关键词： R-(+)-长叶薄荷酮腹腔感染耐药性大肠埃希菌抗炎作用

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The Midgut Bacterial Flora of Laboratory-Reared Hard Ticks, Haemaphysalis longicornis, Hyalomma asiaticum, and Rhipicephalus haemaphysaloides

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Journal of Integrative Agriculture 2014年第8期13卷 1766-1771页

作者： LI Chun-hong CAO Jie ZHOU Yong-zhi ZHANG Hou-shuang gong hai-yan ZHOU Jin-lin Key Laboratory of Animal Parasitology Ministry of Agriculture/Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences

Ixodid ticks play an important role in the transmission of a variety of zoonoses of viral, bacterial and protozoan origin, and they also harbor a wealth of microorganisms. To gain more detailed insights into the potential interactions between bacterial flora and tick-borne pathogens, we investigated the midgut bacterial flora of laboratory-reared Haemaphysalis longicornis, Hyalomma asiaticum and Rhipicephalus haemaphysaloides. Based on morphological, biochemical, and 16 S rDNA sequencing results, we identified 15 species belonging to 12 genera in the midgut of the three ticks. The bacterial communities were similar to those found in other studies of hematophagous arthropods. Kocuria sp. was the most frequently isolated species and its 16 S rDNA gene sequence was very similar to Kocuria koreensis P31 T. To our knowledge, this is the first report of the bacterial flora of tick midguts and the results show that there were many different bacterial species in each tick species. Among the most common genera, there may have been a novel species in the genus Kocuria. The results might be the first step for looking for different aspects of the pathogen and tick interaction.

关键词： bacteria Kocuria sp. tick midgut

Proteomic Analysis of Proteins in the Salivary Glands of the Fed and Unfed Female Tick Rhipicephalus haemaphysaloides

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Agricultural Sciences in China 2009年第1期8卷 121-127页

作者： XIANG Fei-yu ZHANG Jian-wu ZHOU Yong-zhi LI Zhuang gong hai-yan ZHOU Jin-lin Key Lab of Animal Parasitology of Ministry of Agriculture of China~Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences Shanghai 200232 P.R.China Shanghai lnsititute of Cardiovascular Diseases Zhongshan Hospital Fudan University Shanghai 200032 P.R.China

Identification of differentially expressed salivary gland proteins between the fed and unfed female Rhipicephalus haemaphysaloides may obtain valuable functional molecules. In this study, comparative two-dimensional gel electrophoresis （2-DE） and mass spectrometry were used to separate and identify differentially expressed salivary gland proteins between the fed and unfed female R. haemaphysaloides. The soluble proteins from the salivary glands of fed and unfed female R. haemaphysaloides were separated by a sequential extraction method followed by 2-DE and 2-DE images. Image analysis of the gels revealed 1 096 ± 87 protein spots from the fed female ticks and 991 ±64 protein spots from the unfed female ticks. Among those protein spots, about 724 ±34 were present both in the fed and unfed female ticks. Fourteen spots from the fed ticks and six spots from the unfed ticks were selected for peptide mass fingerprinting （PMF） and sequencing assay by mass spectrometry （MS）. Bioinformatic analysis showed that a majority of the differentially expressed proteins were involved in signal transduction, metabolism, and transcriptional regulation. These differentially expressed proteins might be antigen candidates for the development of vaccines against the tick.

关键词： Rhipicephalus haemaphysaloides salivary g/and differentially expressed proteins two-dimensional gel electrophoresis mass spectrometry

Development of a novel two color tracer perfusion technique for the hydrodynamic study of aqueous outflow in bovine eyes

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Chinese Medical Journal 2010年第5期123卷 599-605页

作者： ZHU Jing-yin YE Wen gong hai-yan Department of Ophthalmology Boston University School of Medicine Boston MA 02118 USA Department of Ophthalmology Huashan Hospital of Fudan University Shanghai 200040 China Department of Ophthalmology Huadong Hospital of Fudan University Shanghai 200040 China

Background Elevation of intraocular pressure is usually associated with primary open angle glaucoma and caused by increased outflow resistance. A two-color fluorescent tracer technique was developed to investigate the hydrodynamics of aqueous humor outflow with changing intraocular pressure within the same eye, to better understand the relationship between outflow facility and effective filtration area. Methods Eighteen enucleated bovine eyes were first perfused at 30 mmHg with Dulbecco＇s phosphate-buffered saline containing 5.5 mmol/L D-glucose. After a stable baseline facility, red fluorescent microspheres （0.5 um, 0.002% v/v） were exchanged and perfused. Eyes in the one-color control group （n=6） were immediately perfused with fixative. In the experimental group （n=6）, eyes were perfused with green tracer after intraocular pressure reduced to 7 mmHg, while in the two-color control group （n=6）, eyes were perfused with green tracer with intraocular pressure remaining at 30 mmHg. All 12 eyes were then per＇fusion-fixed. Outflow facility was continuously recorded in all eyes. Confocal images were taken along the inner wall of the aqueous plexus and the percent of the effective filtration length （PEFL; length of inner wall exhibiting tracer labeling/total length of inner wall） was measured. The relationships between outflow facility and PEFL were analyzed statistically. Results No significant differences were found in baseline facilities （***-1） among the three groups （the experimental group： 0.93±0.12; the two-color control group： 0.90±0.19; the one-color control group： 0.98±0.13）. In the experimental group, the outflow facility was significantly higher at 7 mmHg （4.29±1.01） than that at 30 mmHg （1.90±0.67, P 〈0.001）, which corresponded to a significant increase in the PEFL at 7 mmHg （54.70±8.42） from that at 30 mmHg （（11.76±4.56）%, P 〈0.001）. The PEFL labeled by red fluorescent microspheres in the experimental group （（11.76±4.56）%） showed no significant diff

关键词： aqueous humor intraocular pressure trabecular meshwork filtration fluorescent, microspheres perfusion

Reversible changes in aqueous outflow facility, hydrodynamics, and morphology following acute intraocular pressure variation in bovine eyes

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Chinese Medical Journal 2013年第8期126卷 1451-1457页

作者： ZHU Jing-ying YE Wen WANG Ti gong hai-yan Department of Ophthahnology Huashan Hospital of FudanUniversity Shanghai 200040 China Department of Ophthalmology Huadong Hospital of FudanUniversity Shanghai 200040 China Department of Ophthahnology Boston University School ofMedicine Boston MA 02118 USA Department of Ophthalmology 85th Hospital of People'sLiberation Army Shanghai 200052 China

Background Elevated intraocular pressure （lOP） is primarily due to increased aqueous outflow resistance, but how aqueous outflow resistance is generated and regulated are still not fully understood. The aim of this study is to determine whether changes in outflow facility, outflow pattern, and morphology following acute lOP elevation were reversible when the lOP was returned to a normal level in bovine eyes using a two-color tracer technique to label outflow patterns within the same eye. Methods Twelve fresh enucleated bovine eyes were perfused with Dulbecco＇s phosphate buffer saline （PBS） containing 5.5 mmol/L glucose （DBG） at 30 mmHg first to establish the baseline outflow facility followed by a fixed volume of red fluorescent microspheres （0.5 μm, 0.002% v/v）. After the red tracer being replaced with DBG in the anterior chamber, perfusion was continued at 7 mmHg with the same volume of green tracer, followed by a fixative. In two control groups, the eyes were constantly perfused at either 30 mmHg （n=6） or 7 mmHg （n=6） using the same methods. The outflow facility （C, ***.-lmmHg-1）, was continuously recorded. Confocal images were taken along the inner wall （IW） of the aqueous plexus （AP） in frontal sections. The percent of the effective filtration length （PEFL, PEFL=IW length exhibiting tracer labeling/total length of IW） was measured. Sections with AP were processed and examined by light microscopy. The total length of IW and the length exhibiting separation （SL） in the juxtacanalicular connective tissue （JCT） were measured. A minimum of eight collector channel （CC） ostia per eye were analyzed for herniations. Results In the experimental （30-7 mmHg） group, the outflow facility was significantly higher at 7 mmHg （（4.81±1.33） #lmin-1 mmHg-1） than that at 30 mmHg （（0.99±0.15） μ***-1 mmHg-1, P=-0.002）, corresponding to a significant increase in the PEFL （P=-0.0003）. The percent of CC ostia exhibiting herniations in the experimental group （（67.40±8.90） μ***·-1mm

关键词： aqueous humor ouUTow Jacility: ocular perfusion intraocular pressure: trabecular meshwork:fluorescent microspheres

Implications of the Stellar Mass Density of High-z Massive Galaxies from JWST on Warm Dark Matter

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Research in Astronomy and Astrophysics 2024年第1期24卷 128-134页

作者： Hengjie Lin yan gong Bin Yue Xuelei Chen National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Science Center for China Space Station Telescope National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China Department of Physics College of SciencesNortheastern UniversityShenyang 110819China Center for High Energy Physics Peking UniversityBeijing 100871China

A significant excess of the stellar mass density at high redshift has been discovered from the early data release of James Webb Space Telescope(JWST),and it may require a high star formation ***,this will lead to large number density of ionizing photons in the epoch of reionization(EoR),so that the reionization history will be changed,which can arise tension with the current EoR *** dark matter(WDM),via the free streaming effect,can suppress the formation of small-scale structure as well as low-mass *** provides an effective way to decrease the ionizing photons when considering a large star formation efficiency in high-z massive galaxies without altering the cosmic reionization *** the other hand,the constraints on the properties of WDM can be derived from the JWST *** this work,we study WDM as a possible solution to reconcile the JWST stellar mass density of high-z massive galaxies and reionization *** find that,the JWST high-z comoving cumulative stellar mass density alone has no significant preference for either CDM or WDM *** using the observational data of other stellar mass density measurements and reionization history,we obtain that the WDM particle mass with mw=0.51_(-0.12)^(+0.22) keV and star formation efficiency parameter f_(*)^(0)> 0.39 in 2σ confidence level can match both the JWST high-z comoving cumulative stellar mass density and the reionization history.

关键词： Cosmology (cosmology:)dark matter (cosmology:)large-scale structure of universe

Lightweight innovation ADIs help development of renewable energy and new technology industries in China

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China Foundry 2024年第5期21卷 507-515页

作者： Qi-dong yan Xu-dong gong Wen-bang gong Jin-cheng Liu ADI Committee of China Foundry Association Beijing 100000China Yuzhou Henglilai New Materials Co. Ltd.Xuchang 452570HenanChina Kunshan Changjian Foundry Industry Co.Ltd. Kunshan 215311JiangsuChina School of Mechanical Engineering and Automation Wuhan Textile UniversityWuhan 430200China WFO Ferrous Metals Commission Shenyang 110022China

The world is undergoing profound changes in energy and *** are vigorously developing new sustainable energy sources and *** energy sources encompass various technologies such as wind turbines,solar energy,nuclear energy,and ***,emerging technology fields include new energy vehicles,robots,and artificial intelligence devices,among *** renewable energy industries and implementation of new technologies necessitate the development and adoption of new equipment and *** ductile iron(ADI)is renowned for its unique microstructure and superior *** utilizing ADI,lightweight and innovative castings can be designed to not only reduce weight but also save energy and decrease *** importantly,these castings enhance the efficiency and reliability of new energy equipment and emerging technology *** paper describes the development,applications,and future prospects of lightweight and innovative ADI castings within sectors such as solar photovoltaic(PV),wind power generation,industry robots,and trucks in China.

关键词： lightweight ADI renewable energy emission reduction

The Role of Nudging in Systems

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Journal of Systems Science and Information 2024年第3期12卷 412-422页

作者： Qiguo gong hai yanG School of Economics and Management University of Chinese Academy of SciencesBeijing 100190China

The 2017 Nobel Prize in Economics was awarded to Thaler,whose concept of nudges has been widely *** existing research has proposed numerous nudging strategies,no research comprehensively examines the role of nudging,resulting limited in attention across various *** fill this gap,this study aims to present how to analyze the role of nudges in the system using a systems thinking *** analysis indicates that nudges have a multiplier effect in the system and can achieve significant results at a small *** use three cases to analyze the effective impact of nudges on decision making:changing the default setting in organ donation,providing the check list to avoid mistakes in surgery,and the approach to prevent the coronavirus epidemic applied in China.

关键词： nudging system

Phase modulation of nickel-tin alloys in regulating electrocatalytic nitrogen reduction properties

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Rare Metals 2024年第6期43卷 2851-2858页

作者： Lin Wang Hang-Ning Liu Xin Meng Chang-Hui Sun hai-Dong Liu Liang-Yu gong Zhen-Hua yan Jie Wang College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural UniversityQingdao 266109China Department of Chemistry-Angstrom Laboratory Uppsala University75121UppsalaSweden Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) College of ChemistryNankai UniversityTianjin 300071China

Electrocatalytic nitrogen reduction reaction(NRR)is one of the attractive methods for ammonia(NH_(3))production at ambient conditions,but the catalytic performance largely depends on the physical properties of the ***,Ni-Sn alloy nanoparticles with well-controlled physical properties have been synthesized via a simple two-step method,which are applied as efficient electrocatalysts for the NRR.

关键词： alloys alloy nickel