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Metagenomics reveals the microbial community and functional metabolism variation in the partial nitritation-anammox process:From collapse to recovery

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Journal of Environmental Sciences 2024年第1期135卷 210-221页

作者： Hong Wang Hui gong Xiaohu dai Min Yang State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and EngineeringTongji UniversityShanghai 200092China BIOMATH Department of Data Analysis and Mathematical ModellingGhent UniversityGhent 9000Belgium

Mainstream partial nitritation-anammox(PNA)process easily suffers from performance instability and even reactor collapse in ***,it is of great significance to unveil the characteristic of performance recovery,understand the intrinsic mechanism and then propose operational *** this study,we combined long-term reactor operation,batch tests,and metagenomics to reveal the succession of microbial community and functional metabolism variation from system collapse to *** aeration control(0.10-0.25mg O_(2)/L)was critical for performance *** was also found that Candidatus Brocadia became the dominant flora and its abundance increased from 3.5%to 11.0%.Significant enhancements in carbon metabolism and phospholipid biosynthesis were observed during system recovery,and the genes abundance related to signal transduction was dramatically *** up-regulation of sdh and suc genes showed the processes of succinate dehydrogenation and succinyl-CoA synthesis might stimulate the production of amino acids and the synthesis of proteins,thereby possibly improving the activity and abundance of AnAOB,which was conducive to the performance ***,the increase in abundance of hzs and hdh genes suggested the enhancement of the anammox *** in the abundance of key genes involved in nitrogen metabolism indicated that nitrogen removal pathway was more diverse after system *** achievement of performance recovery was driven by anammox,nitrification and denitrification coupled with dissimilatory nitrate reduction to *** results provide deeper insights into the recovery mechanism of PNA system and also provide a potential regulation strategy for the stable operation of the mainstream PNA process.

关键词： Partial nitritation-anammox Metagenomics Microbial community Functional metabolism Recovery

Defects in phospholipase C zeta cause polyspermy and low fertilization after conventional IVF:not just ICSI failure

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Asian Journal of Andrology 2024年第2期26卷 175-182页

作者： Jian-Fang Che Hui-Xia Wu Si-Cong Zeng Yue-Ren Wu Jing dai De-Hua Cheng Fei gong guang-Xiu Lu Ge Lin Can dai School of Medicine Hunan Normal UniversityChangsha 410013China Reproductive and Genetic Hospital of CITIC-Xiangya Changsha 410008China Clinical Research Center for Reproduction and Genetics in Hunan Province Changsha 410078China National Engineering and Research Center of Human Stem Cell Changsha 410205China

Phospholipase C zeta(PLC)is a key sperm-borne oocyte-activating factor that triggers Ca^(2+)oscillations and the subsequent block to polyspermy following gamete *** in PLCZ1,the gene encoding PLCζ,cause male infertility and intracytoplasmic sperm injection(ICSI)fertilization failure;and PLCζ expression and localization patterns are significantly correlated with ICSI fertilization rate(FR).However,in conventional in vitro fertilization(cIVF),whether and how sperm PLCζ affects fertilization remain ***,we identified one previously reported and two novel PLCZ1 mutations associated with polyspermy in vitro that are characterized by excessive sperm-zona binding and a delay in pronuclei(PN)*** staining and oocyte activation testing revealed that virtually all spermatozoa from patients lacked functional PLCζ and were thus unable to evoke Ca^(2+) *** with an artificial oocyte activation treatment successfully rescued the polyspermic phenotype and resulted in a live ***,we analyzed PLCζ in an additional 58 males after cIVF treatment in the Reproductive and Genetic Hospital of CiTiC-Xiangya(Changsha,China)between February 2019 and January *** found that the proportion of spermatozoa that expressed PLCζ was positively correlated with both 2PN rate and total *** optimal cutoff value below which males were likely to experience low FR(total FR≤30%)after clVF was 56.7%for the proportion of spermatozoa expressing *** study expands the mutation and the phenotypic spectrum of PLCZ1 and further suggests that PLCζ constitutes a promising biomarker for identifying low FRs cases in cIVF due to sperm-related oocyte activation deficiency and that sperm PLCζ analysis may benefit the widermale population and not onlymen with IcsI failure.

关键词： conventional IVF gene variants PLCζ polyspermy

A Constraint Adaptive Multi-Tasking Differential Evolution Algorithm:Designed for Dispatch of Integrated Energy System in Coal Mine

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Tsinghua Science and Technology 2024年第2期29卷 368-385页

作者： Canyun dai Xiaoyan Sun Hejuan Hu Yong Zhang Dunwei gong School of Information and Control Engineering China University of Mining and TechnologyXuzhou 221116China School of Information Science and Technology Qingdao University of Science and TechnologyQingdao 266061China

The dispatch of integrated energy systems in coal mines(IES-CM)with mine-associated supplies is vital for efficient energy utilization and carbon emissions ***,IES-CM dispatch is highly challenging due to its feature as multi-objective and strong *** constrained multi-objective evolutionary algorithms often fall into locally feasible domains with poorly distributed Pareto front,which greatly deteriorates dispatch *** tackle this problem,we transform the traditional dispatch model of IES-CM into two tasks:the main task with all constraints and the helper task with constraint *** we propose a constraint adaptive multi-tasking differential evolution algorithm(CA-MTDE)to optimize these two tasks *** helper task with constraint adaptive is developed to obtain infeasible solutions near the feasible *** purpose of this infeasible solution is to transfer guiding knowledge to help the main task move away from local ***,a dynamic dual-learning strategy using DE/current-to-rand/1 and DE/current-to-best/1 is developed to maintain task diversity and ***,we comprehensively evaluate the performance of CA-MTDE by applying it to a coal mine in Shanxi Province,considering two IES-CM *** demonstrate the feasibility of CA-MTDE and its ability to generate a Pareto front with exceptional convergence,diversity,and distribution.

关键词： dispatch integrated energy system coal mine evolutionary multi-tasking constraint differential evolution

Online task planning method of anti-ship missile based on rolling optimization

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Journal of Systems Engineering and Electronics 2024年第3期35卷 720-731页

作者： LU Faxing dai Qiuyang YANG guang JIA Zhengrong College of Weaponry Engineering Naval University of EngineeringWuhan 430033China

Based on the wave attack task planning method in static complex environment and the rolling optimization framework, an online task planning method in dynamic complex environment based on rolling optimization is proposed. In the process of online task planning in dynamic complex environment,online task planning is based on event triggering including target information update event, new target addition event, target failure event, weapon failure event, etc., and the methods include defense area reanalysis, parameter space update, and mission re-planning. Simulation is conducted for different events and the result shows that the index value of the attack scenario after re-planning is better than that before re-planning and according to the probability distribution of statistical simulation method, the index value distribution after re-planning is obviously in the region of high index value, and the index value gap before and after re-planning is related to the degree of posture change.

关键词： target allocation of anti-ship missile defense area rolling optimization task re-planning

DLF-YOLOF:an improved YOLOF-based surface defect detection for steel plate

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Journal of Iron and Steel Research International 2024年第2期31卷 442-451页

作者： guang-hu Liu Mao-xiang Chu Rong-fen gong Ze-hao Zheng School of Electronic and Information Engineering University of Science and Technology LiaoningAnshan114051LiaoningChina

Surface defects can affect the quality of steel *** methods based on computer vision are currently applied to surface defect detection of steel ***,their real-time performance and object detection of small defect are still *** improved object detection network based on You Only Look One-level Feature(YOLOF)is proposed to show excellent performance in surface defect detection of steel plate,called ***,the anchor-free detector is used to reduce the network ***,deformable convolution network and local spatial attention module are introduced into the feature extraction network to increase the contextual information in the feature ***,the soft non-maximum suppression is used to improve detection accuracy ***,data augmentation is performed for small defect objects during training to improve detection *** show the average precision and average precision for small objects are 42.7%and 33.5%at a detection speed of 62 frames per second on a single GPU,*** shows that DLF-YOLOF has excellent performance to meet the needs of industrial real-time detection.

关键词： Steel surface defects detection YOLOF Anchor-free detector Small object detection Real-time detection

Cr^(3+) activated Na_(3)RESi_(3)O_(9)(RE=Y,Lu,Sc) silicate broadband near-infrared phosphors for luminescence towards NIR-Ⅱ region via a multi-site occupancy strategy

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Journal of Rare Earths 2024年第8期42卷 1447-1457,I0002页

作者： Jiantong Wang Changshuai gong Sihan Yang Qi Zhu Xuejiao Wang Ji-guang Li College of Chemistry and Materials Engineering Bohai UniversityJinzhou121007China Key Laboratory for Anisotropy and Texture of Materials(Ministry of Education) School of Materials Science and EngineeringNortheastern UniversityShenyang110819China Research Center for Electronic and Optical Materials National Institute for Materials ScienceTsukubaIbaraki 305-0044Japan

Phosphor-converted near-infrared light-emitting diodes(NIR pc-LEDs) are finding applications in various fields including food quality analysis,biomedical imaging,night vision,and *** crucial factor in the development of NIR pc-LEDs devices lies in the advancement of high-performance broadband NIR phosphors,In this work,novel Cr^(3+)-activated silicate phosphors NaRESi_(3)O_(9)(RE=Y,Lu,Sc) are *** silicate has a special 3D network structure in which RE has four different sites,forming four octahedrons and providing suitable occupation sites for Cr^(3+).The phosphors demonstrate a wide emission spectrum ranging from 750 to 1450 nm when excited by light at 468 *** full width at half maximum(FWHM),which benefits from the presence of Cr^(3+)ions occupying multiple sites,is measured to be 203 ***,the strongest emission peak is observed at a longer wavelength of 984 nm compared to most other systems activated by Cr^(3+).The Na_(3)ScSi_(3)O_(9) lattice provides a weak crystal field(Dq/B=1.97) and weak phonon-photon coupling for Cr^(3+),and the integrated emission intensity of Na_(3)ScSi_(3)O_(9):0,03Cr^(3+)is 4.66 times stronger than that of Na_(3)YSi_(3)O_(9):0.03Cr^(3+).

关键词： Rare earths Broadband near-infrared Silicate Photoluminescence Cr^(3+)

Relative orbit determination algorithm of space targets with passive observation

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Journal of Systems Engineering and Electronics 2024年第3期35卷 793-804页

作者： dai Chenchao QIANG Hongfu ZHANG Degang HU Shaolei gong Baichun Zhijian Laboratory Rocket Force University of EngineeringXi’an 710025China College of Astronautics Nanjing University of Aeronautics and AstronauticsNanjing 210016China China Flight Test Research Institute Xi’an 710089China Beijing Institute of Control and Electronic Technology Beijing 100038China

Angles-only relative orbit determination for space non-cooperative targets based on passive sensor is subject to weakly observable problem of the relative state between two spacecraft. Previously, the evidence for angles-only observability was found by using cylindrical dynamics, however, the solution of orbit determination is still not provided. This study develops a relative orbit determination algorithm with the cylindrical dynamics based on differential evolution. Firstly, the relative motion dynamics and line-of-sight measurement model for nearcircular orbit are established in cylindrical coordinate ***, the observability is qualitatively analyzed by using the dynamics and measurement model where the unobservable geometry is found. Then, the angles-only relative orbit determination problem is modeled into an optimal searching frame and an improved differential evolution algorithm is introduced to solve the problem. Finally, the proposed algorithm is verified and tested by a set of numerical simulations in the context of highEarth and low-Earth cases. The results show that initial relative orbit determination(IROD) solution with an appropriate accuracy in a relative short span is achieved, which can be used to initialize the navigation filter.

关键词： angles-only measurement relative orbit determination cylindrical coordinate differential evolution

Classification and rating of disintegrated dolomite strata for slope stability analysis

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第7期16卷 2552-2562页

作者： Wenlian Liu Xinyue gong Jiaxing Dong Hanhua Xu Peixuan dai Shengwei Zhang Kunming Prospecting Design Institute of China Nonferrous Metals Industry Co. Ltd.Kunming650051China Key Laboratory of Geotechnical Engineering and Geological Disasters of Yunnan Province Kunming650051China Faculty of Electric Power Engineering Kunming University of Science and TechnologyKunming650500China

Although disintegrated dolomite,widely distributed across the globe,has conventionally been a focus of research in underground engineering,the issue of slope stability issues in disintegrated dolomite strata is gaining increasing *** is primarily due to their unique properties,including low strength and loose *** methods for evaluating slope stability,such as basic quality(BQ)and slope stability probability classification(SSPC),do not adequately account for the poor integrity and structural fragmentation characteristic of disintegrated *** address this challenge,an analysis of the applicability of the limit equilibrium method(LEM),BQ,and SSPC methods was conducted on eight disintegrated dolomite slopes located in Baoshan,Southwest ***,conflicting results were ***,this paper introduces a novel method,SMRDDS,to provide rapid and accurate assessment of disintegrated dolomite slope *** method incorporates parameters such as disintegrated grade,joint state,groundwater conditions,and excavation *** findings reveal that six slopes exhibit stability,while two are considered partially ***,the proposed method demonstrates a closer match with the actual conditions and is more time-efficient compared with the BQ and SSPC ***,due to the limited research on disintegrated dolomite slopes,the results of the SMRDDS method tend to be conservative as a safety *** conclusion,the SMRDDS method can quickly evaluate the current situation of disintegrated dolomite slopes in the *** contributes significantly to disaster risk reduction for disintegrated dolomite slopes.

关键词： Disintegrated dolomite slope Basic quality(BQ) Slope stability probability classification (SSPC) Rock mass quality classification Limit equilibrium method(LEM)

The effects of various factors on spontaneous imbibition in tight oil reservoirs

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Petroleum Science 2024年第1期21卷 315-326页

作者： Cheng Liu Tian-Ru Wang Qing You Yue-Chun Du guang Zhao Cai-Li dai School of Energy Resources China University of GeosciencesBeijing100083PR China School of Petroleum Engineering China University of PetroleumQingdao266580ShandongPR China

Slickwater fracturing fluids have gained widespread application in the development of tight oil reservoirs. After the fracturing process, the active components present in slickwater can directly induce spontaneous imbibition within the reservoir. Several variables influence the eventual recovery rate within this procedure, including slickwater composition, formation temperature, degree of reservoir fracture development, and the reservoir characteristics. Nonetheless, the underlying mechanisms governing these influences remain relatively understudied. In this investigation, using the Chang-7 block of the Changqing Oilfield as the study site, we employ EM-30 slickwater fracturing fluid to explore the effects of the drag-reducing agent concentration, imbibition temperature, core permeability, and core fracture development on spontaneous imbibition. An elevated drag-reducing agent concentration is observed to diminish the degree of medium and small pore utilization. Furthermore, higher temperatures and an augmented permeability enhance the fluid flow properties, thereby contributing to an increased utilization rate across all pore sizes. Reduced fracture development results in a lower fluid utilization across diverse pore types. This study deepens our understanding of the pivotal factors affecting spontaneous imbibition in tight reservoirs following fracturing. The findings act as theoretical, technical, and scientific foundations for optimizing fracturing strategies in tight oil reservoir transformations.

关键词： Tight oil reservoir Spontaneous imbibition Nuclear magnetic resonance Slickwater fracturing fluid Fluid utilization degree