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Evaluation of FGF10 as a candidate gene for high myopia in a Han Chinese population

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Eye and Vision 2019年第1期6卷 284-290页

作者： Lingxi Jiang Dongyan Luo Tingting Wang Rui Zheng Yaru Zhai Xiaoqi Liu bo gong Zhengzheng Wu Yin Yang Zhenglin Yang Yi Shi Sichuan Provincial Key Laboratory for Human Disease Gene Study Department of Clinical LaboratorySichuan Academy of Medical Sciences&Sichuan Provincial People’s HospitalSchool of MedicineUniversity of Electronic Science and Technology of China32 the First Ring Road West 2Chengdu 610072SichuanChina Department of Ophthalmology Sichuan Academy of Medical Sciences&Sichuan Provincial People’s HospitalChengduChina. Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital ChengduSichuanChina

Background:Fibroblast growth factor 10(FGF10)is implicated in the growth and development of the *** singles nucleotide polymorphisms(SNPs)in the FGF10 gene(including rs1384449,rs339501,rs12517396 and rs10462070)were found to be associated with extreme myopia(EM,refractive error≤−10.0 diopters)in Japanese and Chinese Taiwan *** case-control association study was conducted to explore the relationship between these four SNPs and high myopia in a western Chinese ***:A total of 869 high myopia patients(HM,including 485 EM patients)and 899 healthy controls were *** four SNPs were genotyped using the ABI SNaPshot *** genetic models(allelic,homozygous,heterozygous,dominant,and recessive)were applied to further evaluate the possible correlation between the SNPs and high *** linkage-disequilibrium block(LD)structure was tested by Haploview ***:In our study,no statistically significant differences were found between HM/EM patients and controls after bonferroni multiple-correction(P>0.05)in the allele frequencies of these four SNPs in the FGF10 *** further found that rs12517396AA and rs10462070GG carriers showed a decreased risk of HM/EM compared with rs12517396AC+CC and rs10462070GA+AA carriers(P=0.045,OR=0.366;P=0.021,OR=0.131;P=0.03,OR=0.341;P=0.015,OR=0.122;respectively).Additionally,rs12517396AA and rs10462070GG carriers showed the same decreased risk of HM/EM compared with rs12517396CC and rs10462070AA carriers(P=0.048,OR=0.370;P=0.023,OR=0.133;P=0.032,OR=0.346;P=0.017,OR=0.126).However,these significant associations between rs12517396/rs10462070 and HM/EM disappeared after bonferroni multiple-correction(P>0.05).Conclusion:Our findings indicate that rs12517396 and rs10462070 had marginal association with HM and *** other two common polymorphisms in FGF10 unlikely have significant effects in the genetic predisposition to HM/EM in western Chinese *** replication studies are needed

关键词： High myopia Extreme myopia Association study Fibroblast growth factor 10

A series of stable, metastable and unstable salts of Imatinib with improved solubility

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Chinese Chemical Letters 2022年第4期33卷 2159-2164页

作者： Zheng-Yu Fang Bao-Xi Zhang Wen-Hui Xing Hong-Li Jia Xue Wang Ning-bo gong Yang Lu Guan-Hua Du Beijing Key Laboratory of Polymorphic Drugs Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191 China Beijing City Key Laboratory of Drug Target Identification and Drug Screening Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

Pharmaceutical salt formation is the most preferred and effective method to enhance the physicochemical properties of APIs. The aim of the study was to design and synthesize a series of new salts to improve the solubility of Imatinib(IM). Two stable salts with malonic acid(S1) and citric acid(S5), one metastable salt with fumaric acid(S2), two unstable salts with citric acid(S3, S4) were obtained for the first *** crystal and powder X-ray diffraction, Fourier transform infrared, differential scanning calorimetry,and thermogravimetric analysis were used to characterize the novel salts. The solubility and stability of the solid were also evaluated, and three salts(S1, S2, S5) had a more than 20 folds of solubility and a faster dissolution rate improved as compared to the pure drug in water and p H 6.8 buffer, respectively.

关键词： Imatinib Salt Characterization Stability Solubility

Epigenetic and transcriptional activation of the secretory kinase FAM20C as an oncogene in glioma

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Journal of Genetics and Genomics 2023年第6期50卷 422-433页

作者： bo gong Yi Liang Qian Zhang Huan Li Jialing Xiao Liang Wang Han Chen Wenjie Yang Xiaoqing Wang Yi Wang Zongze He Department of Health Management Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China The Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Department of Neurology Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Department of Neurosurgery Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Department of Pathology Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Department of Critical Care Medicine Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan610072China

Gliomas are the most prevalent and aggressive malignancies of the nervous *** bioinformatic studies have revealed the crucial role of the secretory pathway kinase FAM20C in the prediction of glioma invasion and ***,little is known about the pathogenesis of FAM20C in the regulation of ***,we construct the full-length transcriptome atlas in paired gliomas and observe that 22 genes are upregulated by full-length transcriptome and differential APA *** of ATAC-seq data reveals that both FAM20C and NPTN are the hub genes with chromatin openness and differential ***,in vitro and in vivo studies suggest that FAM20C stimulates the proliferation and metastasis of glioma ***,NPTN,a novel cancer suppressor gene,counteracts the function of FAM20C by inhibiting both the proliferation and migration of *** blockade of FAM20C by neutralizing antibodies results in the regression of xenograft ***,MAX,BRD4,MYC,and REST are found to be the potential trans-active factors for the regulation of *** together,our results uncover the oncogenic role of FAM20C in glioma and shed new light on the treatment of glioma by abolishing FAM20C.

关键词： Glioma Alternativepolyadenylation sites FAM20C Trans-activefactors

The Mechanism of Heating Rate on the Secondary Recrystallization Evolution in Grain Oriented Silicon Steel

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 275-282页

作者： GAO Qian LI Jun WANG Xianhui CAO Laifu gong Jian LI bo Central Iron and Steel Research Institute Group Beijing 100081China Shougang Zhixin Electromagnetic Material R&D(Beijing)Co. Ltd.Beijing 101300China Beijing Shougang Co. Ltd.Qian’an 064404China

Grain-oriented silicon steels were prepared at different heating rates during high temperature annealing,in which the evolution of magnetic properties,grain orientations and precipitates were *** illustrate the Zener factor,the diameter and number density of precipitates of interrupted testing samples were statistically *** effect of precipitate ripening on the Goss texture and magnetic property was *** indicated that the trend of Zener factor was similar under different heating rates,first increasing and then decreasing,and that the precipitate maturing was greatly inhibited as the heating rate *** recrystallization was developed at the temperature of 1010℃when a heating rate of 5℃/h was used,resulting in Goss,Brass and{110}oriented grains growing abnormally and a magnetic induction intensity of ***,increasing the heating rate to 20℃/h would inhibit the development of undesirable oriented grains and obtain a sharp Goss ***,when the heating rate was extremely fast,such as 40℃/h,poor secondary recrystallization was developed with many island grains,corresponding to a decrease in magnetic induction intensity to 1.87 *** a suitable heating rate of 20℃/h,the sharpest Goss texture and the highest magnetic induction of 1.94 T with an onset secondary recrystallization temperature of 1020℃were found among the experimental variables in this *** heating rate affected the initial temperature of secondary recrystallization by controlling the maturation of precipitates,leading to the deviation and dispersion of Goss texture,thereby reducing the magnetic properties.

关键词： high temperature annealing heating rate secondary recrystallization grain oriented silicon steel

Novel distribution pattern of fibrinolytic components in rabbit tissues extract: a preliminary study

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第8期8卷 570-574页

作者： LU Xing-guo WU Xian-guo XU Xiao-hua gong Xu-bo ZHOU Xuan XU Gen-bo ZHU Lei ZHAO Xiao-ying Department of Haematology the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Department of Clinical Laboratory Diagnosis the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Objective: The purpose of this work was to investigate the distribution pattern of fibrinolytic factors and their inhibitors in rabbit tissues. Methods: The components of the fibrinolytic system in extracts from a variety of rabbit tissues, including tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), plasminogen (Plg), plasmin (Pl) and α2 plasmin inhibitor (α2PI), were determined by colorimetric assay. Results: The tissue extracts in renal, small intestine, lung, brain and spleen demonstrated strong fibrinolytic function, in which high activity of tPA, Plg and Pl was manifested; whereas in skeletal muscle, tongue and stomach, higher activity of PAI-1 and α2PI showed obviously. Also excellent linear correlations were found between levels of tPA and PAI-1, Pl and α2PI, Plg and Pl. In related tissues, renal cortex and renal marrow showed distinctly higher activity of tPA and lower activity of PAI-1, with the levels of Plg and Pl in renal cortex being higher than those in renal marrow, where the α2PI level was higher than that in renal cortex. Similarly, the levels of tPA, Plg and Pl in small intestine were higher than those in large intestine, but with respect to PAI-1 and α2PI, the matter was reverse. In addition, the fibrinolytic activity in muscle tissue was lower, however, the levels of tPA, Plg, and Pl in cardiac muscle were obviously higher than those in skeletal muscles, and the levels of PAI-1 and α2PI were significantly lower than those in skeletal muscle. Conclusion: Our data demonstrate that a remarkable difference of the fibrinolytic patterns exists in rabbit tissues, which has probable profound significance in understanding the relationship between the function of haemostasis or thrombosis and the physiologic function in tissues.

关键词： Tissue extract Fibrinolytic factors Fibrinolytic inhibitors

Sodium 4-phenylbutyrate ameliorates the effects of cataract-causing mutant gammaD-crystallin in cultured cells

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Sodium 4-phenylbutyrate ameliorates the effects of cataract-...

Association analysis of genetic variants in critical patients with COVID-19 and validation in a Chinese population

中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： bo gong Sichuan people

Purpose gammaD-Crystallin(CRYGD) is a major structural lens crystallin and its mutations result in congenital cataract *** this study,we attempted to correct the altered protein features of G165fsX8 CRYGD protein with small chemical molecules. Methods Recombinant FLAG-tagged mutants (R15C,R15S,P24T,R61C and G165fsX8) CRYGD were expressed in COS-7 cells and treated

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来源： cnki会议评论

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Virologica Sinica 2024年第2期39卷 347-350页

作者： Yi Yin Yi Zhang Lelin Sun Shuqiang Wang Yong Zeng bo gong Lulin Huang Yongquan He Zhenglin Yang Center for Natural Products Research Chengdu Institute of BiologyChinese Academy of SciencesChengdu610213China Sichuan Provincial Key Laboratory for Human Disease Gene Study Center for Medical GeneticsDepartment of Laboratory MedicineSichuan Academy of Medical Sciences&Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu610072China Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences(2019RU026) Sichuan Academy of Medical Sciences&Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu610072China University of Chinese Academy of Sciences Beijing100049China Infectious Disease Department Sichuan Academy of Medical Sciences&Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu610072China Department of Ophthalmology Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu610072China

Dear Editor,Genome-wide association studies(GWASs)have reported a genetic association between certain populations and the severity of *** severe patients,changes in the locus 3p21.31(including SLC6A20,LZTFL1,CCR9,FYCO1,CXCR6,and XCR1)are associated with COVID-19 hospitalization(Ellinghaus et al.,2020).Moreover,variations in 9p34.2 region(including genes related to the Abo blood grouping)can affect both susceptibility and severity of COVID-19;in particular,individuals with blood group A may have an increased risk of COVID-19 infection,while those with blood group O may have a protective effect(Ellinghaus et al.,2020;Shelton et al.,2021).

关键词： patients protective

Expression of early growth response factor-1 in rats with cerulein-induced acute pancreatitis and its significance

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World Journal of Gastroenterology 2005年第32期11卷 5022-5024页

作者： Lan-bo gong Li He Yang Liu Xue-Qing Chen bo Jiang Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou 510515 Guangdong Province China Department of Gastroenterology Second Affiliated Hospital of Shenyang Medical College Shenyang 110002 Liaoning Province China

AIM： To observe the expressions of early growth response factor-1 （Egr-1） and tissue factor （TF） in rats with cerulein-induced acute pancreatitis and to explore its significance. METHODS： A large dose of cerulein was used to create the experimental acute pancreatitis model in rats. The changes of Egr-1 mRNA and protein in rats were observed during 30 min to 4 h after the treatment and immunohistochemical method was used to observe the localized expression of Egr-1 in tissues. In addition to the mRNA expression of Egr-1 target gene, TF was also observed. A blank control group, and a bombesinadministered group were used for comparison. RESULTS： Alter the stimulation of a large dose of cerulein, the rats showed typical inflammatory changes of acute pancreatitis. Thirty minutes alter the stimulation, the mRNA expression of Egr-1 in the pancreatic tissue reached its peak and then declined, while the expression of Egr-1 protein reached its peak 2 h after the stimulation. Histologically, 2 h after the stimulation, almost all pancreatic acinar cells had the expression of Egr-1 protein, which was focused in the nuclei. The mRNA expression of TF occurred 1 h after the stimulation and gradually increased within 4 h. However, a large dose of bombesin only stimulated the pancreatic tissue to produce a little mRNA expression of Egr-1 and no mRNA expression of Egr-1 protein and TF. CONCLUSION： Egr-1 as a pro-inflammatory transcription factor may play an important role in the pathogenesis of acute pancreatitis by modulating the expression of TF.

关键词： Growth response factor-1 Tissue factor Acute pancreatitis Cerulein bombesin

Study on Removal of Trace Lead from Nickel Sulphate Solution by Ion-exchange Method

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Journal of Central South University 2000年第4期12卷 191-193页

作者： XIAO Lian-sheng ZHANG Gui-qing ZHANG Qi-xiu gong bo-fan MA Jing-xia LI Ji-zhong Zhu Rui-lun Metallurgical Separation Science and Engineering Lab. Central South UniversityChangsha 410083China Jinchuan Non-ferrousMetals Company Jinchuan737104China

By comparing the adsorption capacity of several kinds of resins, D363 resin is regarded as the most suitable one for removing lead from nickel sulphate electrolyte. The effects of pH, temperature and contact time on exchange adsorption during removing trace lead from industrial nickel sulphate solution with D363 large pore weak alkali anion exchange resin are discussed. Optimum conditions of adsorption of lead from nickel electrolyte by D363 resin are at room temperature, pH 1 2, contact time 30 min.

关键词： ion-exchange nickel sulphate lead