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Preoperative evaluation and outcome of corneal transplantation for limbal dermoids：aten-yearfollow-up study

International Journal of Ophthalmology(English edition) 2016年第12期9卷 1756-1760页

作者： Meng Xin Yue-rong gong Shan-Hao Jiang Chun-Hua Dai Su-Xia Li Wei-Yun Shi Shandong University Jinan 250012 Shandong ProvinceChina Department of Ophthalmology Yantai Affiliated Hospital of Binzhou Medical University Yantai 264100 Shandong Province China Department of Ophthalmology Binzhou Medical University Hospital Binzhou 256603 Shandong Province China Shandong Eye Hospital Shandong Eye Institute Shandong Academy of Medical Sciences Jinan 250021 Shandong Province China

AIM： To summarize preoperative evaluation and outcome of corneal transplantation for limbal dermoids for ten ***： Eighty-five patients diagnosed with limbal dermoids and treated with corneal transplantation were analyzed retrospectively. All patients were further divided into two groups according to absence or presence of neovascularization surrounding the dermoids in the corneal stroma. Eighty-two eyes were treated with tumor excision combined with partial lamellar sclerokeratoplasty, and the other three eyes were performed by penetrating keratoplasty. The size and location of the tumor, the associated ocular and systemic anomalies, the depth of the corneal penetration of tumor tissues, the preoperative and postoperative best-corrected visual acuity （BCVA）, graft survival and cosmetic outcome, and surgical complications were recorded ***： The average age at surgery was 5.3y （range, 3mo-36y）. The mean size of dermoids was 6.1±1.6 mm. The 43.5% of eyes （37/85） were present with hair at the surface of the dermoid and 72.9% of dermoids were located inferotemporal of the eye. Amplyopia was present in 34.1% of patients （29/85） and 9.4% of patients （8/85） had lipodermoids. Eighteen patients suffered from Goldenhar’s syndrome with an accessory ear. The 75% of patients in group 1 had involvement of the corneal deep stroma down to Descemet’s membrane without involving it, but 71.4% of patients had Descemet’s membrane involvement in group 2. Preoperative BCVA ranged from counting fingers to 20/20. Postoperatively 81.1% had a BCVA of 20/800 or better. There was no significant difference between the post-surgical BCVA of the two groups （t=1.584, P〉0.05）. The grafts of 70.5% patients were present as 1＋ opacity, 21.1% as 2＋ opacity, 8.2% as 3＋ opacity and none as 4＋ opacity. Surgical complications included graft rejection, microperforation, prolonged reepithelialization, steroid glaucoma, interface neovascularization, and interface ***

关键词： limbal dermoids corneal transplantation neovascularization

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Plasmon-enhanced photocatalytic oxidative coupling of amines in the air using a delicate Ag nanowire@NH_(2)-UiO-66 core-shell nanostructures

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Chinese Chemical Letters 2024年第4期35卷 506-510页

作者： Yuan Zhang Shenghao gong A.R.Mahammed Shaheer rong Cao Tianfu Liu Fujian Science Technology Innovation Laboratory for Optoelectronic Information of China Fuzhou 350108China College of Chemistry and Materials Science Fujian Normal UniversityFuzhou 350007China State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China

MOF-based core-shell structures with high surface area, abundant active sites, and broad absorption bands are viable alternatives to traditional single-component photocatalysts. In this report, we describe the design and construction of delicate Ag nanowires@NH_(2)-UiO-66 with a core-shell structure for use as photocatalysts in imine synthesis under light. The optimized composites exhibited 80% imine production, which was higher than both MOF and Ag NWs. The significant improvement in photocatalytic activity under light may be attributed to the plasmonic effect of silver nanowires and their core-shell structure, which promotes the separation of electron-hole pairs. Moreover, the photocatalytic activity of the core-shell nanostructure may provide valuable insight into the design and construction of MOF-based composite photocatalysts for oxidative coupling of amines.

关键词： Metal-organic frameworks Plasmonic silver nanowires Photocatalyst Core-shell nanostructure Oxidative coupling of amines

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Production of Functional Materials Derived from Regenerated Silk Fibroin by Utilizing 3D Printing and Biomimetic Enzyme-induced Mineralization

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Chinese Journal of Polymer Science 2024年第3期42卷 299-310,I0005页

作者： Ni Chen Fei-Yu Luo gong-Wen Yang Jin-rong Yao Xin Chen Zheng-Zhong Shao State Key Laboratory of Molecular Engineering of Polymers Laboratory of Advanced Materials and Department of Macromolecular ScienceFudan UniversityShanghai 200433China

Critical-sized bone defects, commonly encountered in clinical orthopedic surgery, present a significant challenge. One of the promising solutions is to prepare synthetic bone substitute materials with precise structural control, mechanical compatibility, and enhanced osteogenic induction performance, nevertheless the successful preparation of such materials remains difficult. In this study, a two-step technique,integrating an extrusion-based printing process with biomimetic mineralization induced by alkaline phosphatase(ALP), was developed. Initially,a pre-cured hydrogel of regenerated silk fibroin(RSF) with a small quantity of hydroxypropyl cellulose(HPC) and ALP was prepared through heating the mixed aqueous solution. This pre-cured hydrogel demonstrated thixotropic property and could be directly extruded into predetermined structures through a 3D-printer. Subsequently, the 3D-printed RSF-based materials with ALP underwent biomimetic in situ mineralization in calcium glycerophosphate(Ca-GP) mineralizing solution, utilizing the polymer chains of RSF as templates and ALP as a trigger for cleaving phosphate bonds of Ca-GP. The resulting 3D-printed RSF-mineral composites including hydrogel and sponge possessed adjustable compression modulus of megapascal grade and variable hydroxyapatite content, which could be controlled by manipulating the duration of the mineralization ***, these 3D-printed RSF-mineral composites demonstrated non-cytotoxicity towards rat bone marrow mesenchymal stem cells. Therefore, they may hold great potential for applications involving the replacement of tissues characterized by osteoinductivity and intricate structures.

关键词： Silk protein Alkaline phosphatase Extrusion-based printing Biomineralization

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Clinical and experimental study on angiopoietin-like protein 8 associated with proliferative diabetic retinopathy

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International Journal of Ophthalmology(English edition) 2017年第12期10卷 1819-1823页

作者： Chang-Xia Dong Cai-Ping Song Chun-Ping Zhang Mei Dong Xiu-rong gong He-Ying Gao Hong Wang Department of Ophthalmology Yantai Yuhuangding HospitalYantai 264000Shandong ProvinceChina Department of Ophthalmology Weihai Municipal HospitalWeihai 264200Shandong ProvinceChina Department of Stomatology Yantai Yuhuangding HospitalYantai 264000Shandong ProvinceChina Department of Ophthalmology Shandong Qilu HospitalShandong UniversityJinan 25000Shandong ProvinceChina

AIM：To confirm the role of angiopoietin-like protein 8（Angptl 8） in proliferative diabetic retinopathy（PDR）.METHODS：The sera and aqueous humor of 10 PDR patients and 10 non-diabetic retinopathy（NDR） patients（idiopathic macular hole patients） were collected and the expression of Angptl 8 was detected by enzyme linked immune-sorbent assay（ELISA）.Experimental diabetes mice model was induced with *** expression of glycosylated hemoglobin and Angptl 8 in sera was *** Angptl 8 was re-infused into wild type（WT） diabetic mice and spatial frequency threshold and contrast sensitivity were *** vitro retinal pigment epithelium（RPE） were stimulated by recombinant Angptl 8 for 24 *** assay were used to detect cell *** the same time,q RT-PCR and Western blot was used to measure the expression of proliferation-related factors in PRE ***：The expression of Angptl 8 was markedly increased in the sera and aqueous humor of PDR patients（F=99.02,P〈0.0001 in sera;t=10.42,P〈0.0001 in aqueous）.After successfully establishing the diabetic mice model,we found that glycosylated hemoglobin and Angptl 8 expression levels were ***-infusion of recombinant Angptl 8 into WT diabetic mice could further decrease spatial frequency threshold and contrast sensitivity（P〈0.01）.In vitro,RPE cells stimulated by recombinant Angptl 8could increase the relative absorbance of MMT assay（1.486±0.042 vs 1.000±0.104,P〈0.05） and proliferating cell nuclear antigen（PCNA） expression（0.55±0.01 vs 0.29±0.03,P〈0.05）.The proliferative effect of Angptl 8 is mainly mediated by increasing the expression of proliferation-activating factors cyclin A1（4.973±0.205 vs 2.720±0.197,P〈0.05）,cyclin F（5.690±0.219 vs 4.297±0.292,P〈0.05） and E2 F2（2.297±0.102 vs 1.750±0.146,P〈0.05）,and reducing the expression of proliferation-inhibiting factors cdkn1（2.370±0.074 vs 3.317±0.135,P〈0.05） and cdkn2（4.793±0.065 vs 5.387±0.149,P〈0.05）.CONCLUSION：The expression of Angptl 8

关键词： angiopoietin-like protein 8 proliferative diabeticretinopathy retinal pigment epithelium proliferation

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Reinforcement of High Impact Polystyrene by Aramid Nanoparticle Fillers Prepared via an In situ Bottom-up Approach

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Chinese Journal of Polymer Science 2024年第8期42卷 1167-1176页

作者： Xiang-Jun gong Zhi-Ping Zhao Chang-Mei Sun Ying Zhang rong-Jun Qu Ying Wang School of Chemistry and Materials Science Ludong UniversityYantai 264025China

In this work,aramid nanoparticles(ANPs)were prepared in dimethyl formamide(DMF)solution containing high impact polystyrene(HIPS)via a bottom-up *** electron microscopy(TEM)images showed that the obtained ANPs were evenly distributed in the HIPS matrix without any *** composition of the ANPs,i.e.,poly(p-phenyl-p-phenylenediamine)(PPTA),was confirmed by Fourier transform infrared spectroscopy(FTIR),X-ray photoelectron spectroscopy(XPS)and X-ray diffractometer(XRD).The ANP/HIPS composites,obtained after ethanol precipitation,were added to neat HIPS as fillers to fabricate ANP/HIPS composite *** surface roughness and the glass transition temperature(T_g)of the sheets were characterized by atomic force microscope(AFM)and differential scanning calorimetry(DSC),*** with neat HIPS,the mechanical properties of the composite sheet were significantly improved,and the Young's modulus increased from 246.55 MPa to 2025.12 MPa,the tensile strength increased from 3.07 MPa to 29.76 MPa,and the toughness increased from 0.32 N/mm^2 to 3.92 N/mm^2,with an increase rate of 721%,869%and 1125%,***,the thermal stability of the composites improved with the increase in ANP content.

关键词： High impact polystyrene PPTA nanoparticle Reinforce Mechanical property

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Changes of the expression of CCNL2 gene during the differentiation of P19 cells to cardiac myocytes

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Journal of Nanjing Medical University 2009年第4期23卷 275-278页

作者： Zhiwen Jiang Lili Zhuo Jie gong rong Yang Yanhui Sheng Xiangqing Kong Kejiang Cao Department of Cardiology the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China

Objective： To investigate the changes of cyclin L2（CCNL2） gene mRNA and protein during the differentiation of P19 cells to cardiac myocytes, and to explore the relationship between CCNL2 gene and the differentiation of cardiac myocytes. Methods： P19 cells were cultured with 0.9% DMSO for 18 days. Western blots of cardiac troponin I （cTnI） were used to identify cell differentiation. Total RNA was extracted from P19 cells during the process of differentiation at various time points：pre-differentiation（Day 0）, and Day 1 to Day 18. The expression levels of CCNL2 gene mRNA and protein were evaluated by RT-PCR and Western blot, respectively. Results： After being induced to differentiate by DMSO for 4 days in suspension, spontaneously and rhythmically beating ceils were seen at 8 day, which were cTnI-positive. In P19 cells, both the expression level of CCNL2 gene mRNA and protein were gradually down-regulated. Conclusion： Both the expression of CCNL2 gene and protein were down-regulated during the process of the differentiation of P19 cells into cardiac myocytes, suggesting a possible role for this cyclin in their differentiation.

关键词： cyclin L2 protein gene P19 cells cell differentiation

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富水多变灰岩-砾石地层顶管施工地表变形规律及机理研究

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地质灾害与环境保护 2024年第3期35卷 95-100页

作者：杨子川徐琬赵宇鹏刘柯李好懿龚玉蓉昆明理工大学国土资源工程学院昆明650093

为探讨研究顶管施工引起的地表变形和对地表建筑物或构筑物产生的影响,以云南昆明某工程顶管施工现场为例,通过对工程现场监测,在富水多变灰岩-砾石地层条件下对顶管施工引起的地表变形规律进行研究。分析监测数据表明,土压力、孔隙水... 详细信息

为探讨研究顶管施工引起的地表变形和对地表建筑物或构筑物产生的影响,以云南昆明某工程顶管施工现场为例,通过对工程现场监测,在富水多变灰岩-砾石地层条件下对顶管施工引起的地表变形规律进行研究。分析监测数据表明,土压力、孔隙水压力与顶管轴线距离呈负相关,影响范围在1.5倍管径内。地表变形为先沉降后隆起再沉降,掘进机机头顶至监测点时隆起值达到最大,影响范围在掘进机前方2倍、掘进机后方3倍顶管深度内。顶管推进过程中,由于土体的固结作用导致部分地面沉降,而灰岩地层中不存在固结效应,因此地表的最终沉降量会随着灰岩比例的增加而减小。该研究为后续减小顶管施工对周围环境的影响提供理论基础。

关键词：顶管地面变形现场监测机理分析

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Stay-Green Panicle Branches Improve Processing Quality of Elite Rice Cultivars

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Rice science 2023年第1期30卷 11-14页

作者： CHEN Yibo ZHAO Lei WANG Chongrong LI Hong HUANG Daoqiang WANG Zhidong ZHOU Degui PAN Yangyang gong rong ZHOU Shaochuan Rice Research Institute Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Laboratory for Lingnan Modern AgricultureGuangzhou 510640China

Rice panicle branches are key routes for transportation of photo synthetic products to *** senescence is an important trait that the breeders considered when breeding elite rice cultivars in South ***,little is known about the mechanisms underlying the contributions of stay-green panicle branches to rice yield and ***,we reported stay-green branches that continuously maintained transportation function and provided carbohydrates for basal grain development at the late stages of rice *** ensured the coordination of development between apical and basal grains,and further improved the processing quality of elite *** findings provided strategies for improving the fullness and quality of rice grains and presented an important basis for cultivating elite rice.

关键词： elite breeding cultivar

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Breeding Effects and Genetic Compositions of a Backbone Parent (Fengbazhan) of Modern indica Rice in China

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Rice science 2022年第5期29卷 397-401,I0001-I0011页

作者： ZHAO Lei ZHOU Shaochuan WANG Chongrong LI Hong HUANG Daoqiang WANG Zhidong ZHOU Degui CHEN Yibo gong rong PAN Yangyang Rice Research Institute Guangdong Academy of Agricultural SciencesGuangdong Laboratory for Lingnan Modern AgricultureGuangdong key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering LaboratoryGuangzhou 510640China

Fengbazhan(FBZ)and its derived varieties have been widely cultivated in China,accounting for 22%of China’s paddy ***,evaluating the *** of FBZ and elucidating its genetic compositions are effective strategies for interpreting the history of modern rice *** integrative bioinformatics analysis and population validation,we found that the expression of favorable genes on chromosome 6 was important for the breeding of FBZ-derived varieties and these favorable genes were gradually optimized during the breeding process,which was consistent with the conjecture of the‘Rice Core Germplasm Breeding Theory’.For several decades,tremendous efforts have been made by Chinese scientists in rice breeding to improve grain yield,nutritional quality and environmental performance,thereby achieving substantial progress in global food *** rice breeding technologies have been developed,including semi-dwarf breeding,utilization of heterosis,launching the‘Super Rice Project’,development of green super rice and molecular design breeding(Qian et al,2016;Bai et al,2018;Tang and Cheng,2018;Yu et al,2022).In southern China,rice can be divided into the early,middle and late rice,and the cultivation area in southern China accounts for approximately 80%of China’s rice cultivation ***,several distinct rice varieties are widely cultivated in southern China.

关键词： breeding paddy dwarf

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借助差热分析研究镍基高温合金/陶瓷型壳界面反应

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铸造技术 2005年第6期26卷 523-524页

作者：龚荣昌贵州安吉航空精密铸造有限责任公司贵州安顺561018

借助差热分析研究镍基高温合金/陶瓷型壳的界面反应,分别从热力学和动力学的角度分析出镍基高温合金叶片在生产过程中陶瓷型壳所需的最佳面层涂料是CoAl2O4。

关键词：差热分析型壳界面反应

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