检索结果-南通市图书馆

Hepatitis C virus infection down-regulates the expression of peroxisome proliferator-activated receptor a and carnitine palmitoyl acyl-CoA transferase 1A

引用

World Journal of Gastroenterology 2005年第48期11卷 7591-7596页

作者： Yang Cheng sébastien Dharancy Mathilde Malapel Pierre Desreumaux Equipe mixte INsERM 0114

AIM： To elucidate the role of the peroxisome proliferator-activated receptor α （PPARα） and its target gene camitine palmitoyl acyl-CoA transferase 2A （CPT1A） in the pathogenesis of hepatitis C virus （HCV） infection.METHODs： Liver samples were collected from the patients with chronic HCV infection and controls. HepG2 cells were transfected with vector pEF352neo carrying. Two independent clones （clone N3 and N4） stably expressing HCV core protein were analyzed. Total RNA was extracted from cells and liver tissues. PPARα and CPT1A mRNAs were quantified by real-time polymerase chain reaction （PCR） using sYBR Green Master. Total extracted proteins were separated by polyacrylamide gel electrophoresis, and electroblotted. Membranes were incubated with the anti-PPARα antibody, then with a swine anti-rabbit IgG conjugated to horseradish peroxidase for PPARα. Protein bands were revealed by an enhanced chemiluminescence reaction for PPARα. For immunohistochemical staining of PPARα, sections were incubated with the primary goat polyclonal antibody directed against PPARα at room temperature.REsULTs： Real-time PCR indicated that the PPARα level and expression level of CPT1A gene in hepatitis C patients lowered significantly as compared with the controls （1.8±2.8 vs 13±3.4, P = 0.0002; 1.1±1.5 vs 7.4±1, P = 0.004）. Western blot results showed that the level of PPARα protein in the livers of hepatitis C patients was lower than that in controls （2.3±0.3 vs 3.6±0.2, P = 0.009）. The immunohistochemical staining results in chronic hepatitis C patients indicated a decrease in PPARα staining in hepatocytes compared with those in the control livers. The in vitro studies found that in the N3 and N4 colon stably expressing HCV core protein, the PPARα mRNA levels were significantly lower than that in the controls.CONCLUsION： The impaired intrahepatic PPARα expression is associated with the pathogenic mechanism in hepatic injury during chronic HCV infection. HCV infection reduced the ex

关键词： Hepatitis C virus Infection PPARα CPT1A

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

sugar-Hormone Cross-Talk in seed Development： Two Redundant Pathways of IAA Biosynthesis Are Regulated Differentially in the Invertase- Deficient miniature 1 （mn 1） seed Mutant in Maize

引用

Molecular Plant 2010年第6期3卷 1026-1036页

作者： Prem s. Chourey Qin-Bao Li Dibyendu Kumar UsDA-ARs Center for Medical Agricultural and Veterinary Entomology Gainesville FL 32608-1069 USA Interdisciplinary Center for Biotechnology Research University of Florida Gainesville FL 32610 USA

The miniature1 （mn1） seed phenotype is a loss-of-function mutation at the Mnl locus that encodes a cell wall invertase; its deficiency leads to pleiotropic changes including altered sugar levels and decreased levels of IAA throughout seed development. To understand the molecular details of such a sugar-hormone relationship, we have initiated studies on IAA biosynthesis genes in developing seeds of maize. Two tryptophan-dependent pathways of IAA biosynthesis, tryptamine （TAM） and indole-3-pyruvic acid （IPA）, are of particular interest. We report on molecular isolation and characterization of an endosperm-specific ZmTARelatedl （ZmTarl） gene of the IPA branch; we have also reported recently on ZmYucl gene in the TAM branch. Comparative gene expression analyses here have shown that （1） the ZmTarl transcripts were approximately 10-fold higher levels than the ZmYucl; （2） although both genes showed the highest level of expression at 8-12 d after pollination （DAP） coincident with an early peak in IAA levels, the two showed highly divergent （antagonistic） response at 12 and 16 DAP but similar patterns at 20 and 28 DAP in the Mnl and ran1 endosperm. The Western blot analyses for the ZmTAR1 protein, however, displayed disconcordant protein/transcript expression patterns. Overall, these data report novel observations on redundant trp-dependent pathways of auxin biosynthesis in developing seeds of maize, and suggest that homeostatic control of IAA in this important sink is highly complex and may be regulated by both sucrose metabolism and developmental signals.

关键词： seed development IAA biosynthesis tryptophan-aminotransferase 1 ZmYUCCA1 gene expression q-PCR maize.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Platelet-rich plasma,an adjuvant biological therapy to assist peripheral nerve repair

引用

Neural Regeneration Research 2017年第1期12卷 47-52页

作者： Mikel sánchez Ane Garate Diego Delgado sabino Padilla Arthroscopic surgery Unit Hospital Vithas San JoseVitoria-GasteizSpain Department of Biomedical Engineering University of MinnesotaMinneapolisMNUSA BTI Biotechnology Institute Vitoria-GasteizSpain

Therapies such as direct tension-free microsurgical repair or transplantation of a nerve autograft,are nowadays used to treat traumatic peripheral nerve injuries（PNI）,focused on the enhancement of the intrinsic regenerative potential of injured ***,these therapies fail to recreate the suitable cellular and molecular microenvironment of peripheral nerve repair and in some cases,the functional recovery of nerve injuries is ***,new biomedical engineering strategies based on tissue engineering approaches through molecular intervention and scaffolding offer promising outcomes on the *** this sense,evidence is accumulating in both,preclinical and clinical settings,indicating that platelet-rich plasma products,and fibrin scaffold obtained from this technology,hold an important therapeutic potential as a neuroprotective,neurogenic and neuroinflammatory therapeutic modulator system,as well as enhancing the sensory and motor functional nerve muscle unit recovery.

关键词： peripheral nerve injuries(PNI) schwann cells axons platelet-rich plasma biomolecules fibrin scaffold intraneural perineural microenvironment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enrichment-ELIsA for Detection of salmonella typhi From Food and Water samples

引用

Biomedical and Environmental sciences 2008年第2期21卷 137-143页

作者： s. KUMAR K. BALAKRIsHNA HV. BATRA Division of Microbiology Defence R & D Establishment Jhansi Road Gwalior 474002 India

Objective Development of monoclonal antibody based sandwich enzyme linked immunosorbant assay （sELIsA） for rapid detection of salmonella enterica serovar typhi （s. typhi） from food and water samples and optimization of enrichment procedures for use with the developed sELIsA to increase the detection sensitivity of the assay. Methods spleen cells from BALB/c mice immunized with flagellin （H=d） antigen of s. typhi were fused with sp2/0 myeloma cells. The hybridoma cell line specific to H=d antigen was established, characterized and ascites raised against one of these clones. The hyperimmune serum to flagellin antigen was raised in New Zealand White rabbits. An sELIsA was developed using polyclonal antibody as capture and monoclonal antibody as detection antibody. To design the efficient culture strategies for use with the sELIsA, different pre-enrichment and enrichment broths were evaluated. The media included buffered peptone water （BPW） and brain heart infusion broth for pre-enrichment and selenite F broth and Rappaport-Vassiliadis broth as enrichment broths. The developed sELIsA with preceding enrichment step in BPW （Enrichment-ELIsA） was evaluated in various food samples artificially inoculated with s. typhi bacteria. Various food （30） and water （35） samples collected from field were also tested by Enrichment-ELIsA and culture method. Results Out of four specific clones to H=d antigen, one clone （# 2/56, IgG2a isotype） was used in sELIsA. The sELIsA had the detection limit of 10^4-10^5 cfu of s. typhi. Of the various broths used with sELIsA, BPW was found to yield maximum ELIsA values. Enrichment-ELIsA, when tested in artificially inoculated food samples, generally, could detect 10^2 s. typhi cfu/mL within 10 h from various food rinses （meat, vegetable） and milk samples. After overnight enrichment in BPW, as less as 2 bacteria per 10 mL of milk, meat rinse, and chicken rinse could be detected. Only one of the field samples （water） gave false positive result by Enr

关键词： Enrichment-ELIsA Food Monoclonal antibody salmonella sandwich ELIsA Water

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identifying female phenotypes that promote behavioral isolation in a sexually dimorphic species of fish Etheostoma zonale

引用

Current Zoology 2021年第2期67卷 225-236页

作者： Natalie s.ROBERTs Tamra C.MENDELsON Department of Biological sciences University of Maryland Baltimore CountyBaltimoreMD21250USA

In sexually dimorphic species characterized by exaggerated male ornamentation,behavioral isola-tion is often attributed to female preferences for conspecific male ***,in a number of sexu-ally dimorphic species,male mate choice also results in behavioral *** many of these cases,the female traits that mediate species boundaries are *** in sexually dimorphic species typically lack many of the elaborate traits that are present in males and that are often used for taxonomic classification of *** a diverse and largely sexually dimorphic group of fishes called darters(Percidae:Etheostoma),male mate choice contributes to behavioral isolation be-tween a number of species;however,studies addressing which female traits males prefer are *** this study,we identified the dominant female pattern for two sympatric species,Etheostoma zonale and Etheostoma barrenense,using pattern energy analysis,and we used discriminate func-tion analysis to identify which aspects of female patterning can reliably classify *** then tested the role of female features in male mate choice for ***,by measuring male preference for computer animations displaying the identified(species specific)conspecific *** found that the region above the lateral line is important in mediating male mate preferences,with males spending a significantly greater proportion of time with animations exhibiting conspecific female patterning in this region than with animations exhibiting heterospecific female *** results suggest that the aspects of female phenotypes that are the target of male mate choice are different from the conspicuous male phenotypes that traditionally characterize species.

关键词： darters discriminant function analysis Etheostoma female phenotype male mate choice 3D animations

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Roles of computational modelling in understanding p53 structure, biology, and its therapeutic targeting

引用

Journal of Molecular Cell Biology 2019年第4期11卷 306-316页

作者： Yaw sing Tan Yasmina Mhoumadi Chandra s. Verma Bioinformatics Institute Agency for Science Technology and Research Singapore 138671 school of Biological sciences Nanyang Technological University Singapore 637551 Department of Biological sciences National University of Singapore Singapore 117543

The transcription factor p53 plays pivotal roles in numerous biological processes, including the suppression of tumours. The rich availability of biophysical data aimed at understanding its structure–function relationships since the 1990s has enabled the application of a variety of computational modelling techniques towards the establishment of mechanistic models. Together they have provided deep insights into the structure, mechanics, energetics, and dynamics of p53. In parallel, the observation that mutations in p53 or changes in its associated pathways characterize several human cancers has resulted in a race to develop therapeutic modulators of p53, some of which have entered clinical trials. This review describes how computational modelling has played key roles in understanding structural-dynamic aspects of p53, formulating hypotheses about domains that are beyond current experimental investigations, and the development of therapeutic molecules that target the p53 pathway.

关键词： p53 structure computational modelling therapeutic targeting

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A study of the temporomandibular joint during bruxism

引用

International Journal of Oral science 2014年第2期6卷 116-123页

作者： María s Commisso Javier Martínez-Reina Juana Mayo Department of Mechanical Engineering University of Seville Seville Spain

A finite element model of the temporomandibular joint （TMJ） and the human mandible was fabricated to study the effect of abnormal loading, such as awake and asleep bruxism, on the articular disc. A quasilinear viscoelastic model was used to simulate the behaviour of the disc. The viscoelastic nature of this tissue is shown to be an important factor when sustained （awake bruxism） or cyclic loading （sleep bruxism） is simulated. From the comparison of the two types of bruxism, it was seen that sustained clenching is the most detrimental activity for the TMJ disc, producing an overload that could lead to severe damage of this tissue.

关键词： bruxism finite element simulation temporomandibular disorder temporomandibular joint

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Extraintestinal manifestations and complications in inflammatory bowel diseases

引用

World Journal of Gastroenterology 2006年第30期12卷 4819-4831页

作者： Katja s Rothfuss Eduard F stange Klaus R Herrlinger Department of Gastroenterology Hepatology and Endocrinology Robert-Bosch-Hospital Stuttgart Germany

Crohn＇s disease （CD） and ulcerative colitis （UC） are chronic inflammatory bowel diseases （IBD） that often involve organs other than those of the gastrointestinal tract. These nonintestinal affections are termed extraintestinal symptoms. Differentiating the true extraintestinal manifestations of inflammatory bowel diseases from secondary extraintestinal complications, caused by malnutrition, chronic inflammation or side effects of therapy, may be difficult. This review concentrates on frequency, clinical presentation and therapeutic implications of extraintestinal symptoms in inflammatory bowel diseases. If possible, extraintestinal manifestations are differentiated from extraintestinal complications. special attention is given to the more recently described sites of involvement; i.e. thromboembolic events, osteoporosis, pulmonary involvement and affection of the central nervous system.

关键词： Inflammatory bowel diseases Crohn's disease Ulcerative colitis Extraintestinal manifestations Complications Therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gamma-ray and neutron shielding efficiency of Pb-free gadolinium-based glasses

引用

Nuclear science and Techniques 2016年第4期27卷 206-213页

作者： V.P.singh N.M.Badiger s.Kothan s.Kaewjaeng T.Korkut H.J.Kim J.Kaewkhao Department of Physics Karnatak University Department of Radiologic Technology Faculty of Associated Medical SciencesChiang Mai University Department of Nuclear Energy Engineering Faculty of Engineering Sinop University Department of Physics Kyungpook National University Center of Excellence in Glass Technology and Materials science Nakhon Pathom Rajabhat University

The radiation shielding efficiency of material depends upon photon attenuation, exposure buildup factors and neutron removal capacity. A newly developed Pb-free gadolinium-based glasses in compositions(80-x) B_2O_3-10 si O_2-10 Ca O-x Gd_2O_3(where x = 15, 20, 25, 30 and35 mol%) had completely been investigated for their shielding efficiency with Geant4 simulation for mass attenuation coefficients and neutron total macroscopic cross section and by calculating exposure buildup *** exposure buildup factors for photon energy from 0.015 to 15 Me V had been calculated up to 40 mean free paths using five factors geometric progression method. The mass attenuation coefficients of the Pb-free glasses were simulated for energies from 223 to 2614 ke V and compared with the possible available experimental results. The neutron shielding efficiency of these glasses was discussed by calculating neutron total macroscopic cross section for energies from 1 e V to 14.1 Me V. Present investigations are found to be very useful for applications in nuclear engineering.

关键词：无铅玻璃屏蔽效率中子质量衰减系数 γ射线光子能量截面计算 Geant4

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Preparation of electroless Ni-P composite coatings containing nano-scattered alumina in presence of polymeric surfactant

引用

Progress in Natural science:Materials International 2012年第4期22卷 318-325页

作者： s.Afroukhteh C.Dehghanian M.Emamy University of Tehran College of EngineeringSchool of Metallurgical and Materials EngineeringTehranIran

Ni-P electroless coating was applied on low carbon steel with the incorporation of different amounts of nano Al2O3 powder (ranging from 3g/l to 30 g/l) in electroless bath. Corrosion properties and microstructures of the coating were studied. The dispersion stability of alumina colloidal particles stabilized by polymeric (non-ionic) surfactants in an electroless bath was also investigated. The surface morphology and the relevant structure were evaluated by scanning electron microscopy (sEM) and X-ray diffraction (XRD). Corrosion behavior of the coated steel was evaluated by electrochemical impedance spectroscopy (EIs) and polarization techniques. The results showed that increasing alumina concentration not only changed the surface morphology, but also promoted the corrosion resistance. Addition of surfactants has an indirect effect on the amount of the incorporated particles. Meanwhile, in the presence of surfactant, corrosion resistance of Ni-P coating containing even a small quantity of alumina was improved since a stabilized bath was obtained.

关键词： Electroless coating Alumina Corrosion resistance surfactant

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：