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Effects of Different Electromagnetic Fields on Circadian Rhythms of Some Haematochemical Parameters in Rats

Biomedical and Environmental Sciences 2009年第4期22卷 348-353页

作者： LAURA CONTALBRIGO CALOGERO STELLETTA LAURA FALCIONI STEFANIA CASELLA giuseppe piccione MORANDO SOFFRITTI AND MASSIMO MORGANTE European Foundation of Oncology and Environmental Sciences "B. Ramazzini" Department of Clinical Veterinary Science University of Padova Department of Experimental Sciences and Applied Biotechnology Faculty of Veterinary Medicine University of Messina

Objective To investigate the effects of different electromagnetic fields on some haematochemical parameters of circadian rhythms in Sprague-Dawley rats. Methods The study was carried out in 18 male and 18 female rats in good health conditions exposed to 50 Hz magnetic sinusoid fields at the intensity of 1000 μT, 100 μT, and 0 μT （control group） respectively, and in 18 male and 18 female rats in good health conditions exposed to 1.8 GHz electromagnetic fields at the intensity of 50 V/m, 25 V/m and 0 V/m （control group）, respectively. Following haematochemical parameters for glucose, triglycerides, and total cholesterol were measured. Results Different effects of electromagnetic fields on circadian rhythms of both male and female rats were observed. Different changes occurred in some haematochemical parameters for glucose, triglycerides, and total cholesterol （P〈0.05）. Conclusion Exposure to different electromagnetic fields is responsible for the variations of some haematochemical parameters in rats.

关键词： Extremely low frequency electromagnetic fields (50 Hz) Radiofrequency electromagnetic fields (1.8 GHz) Haematochemical parameters Circadian rhythm Sprague-Dawley rat

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Effects of Exposure to Extremely Low Frequency Electro-magnetic Fields on Circadian Rhythms and Distribution of Some Leukocyte Differentiation Antigens in Dairy Cows

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Biomedical and Environmental Sciences 2007年第2期20卷 164-170页

作者： CALOGERO STELLETTA PAOLA DE NARDO FRANCESCO SANTIN giuseppe BASSO BARBARA MICHIELOTTO giuseppe piccione MASSIMO MORGANTE Università degli Studi di Padova Dipartimento di Scienze Cliniche VeterinarieViale dell'Università 1635020 Legnaro(PD)Italy Istituto Superiore di Sanità Dipartimento Ambiente e Connessa Prevenzione Primaria Viale Regina Elena 29900161 RomaItaly Università degli Studi di Padova Oncoematologia Pediatrica Via Giustiniani 335128 PadovaItaly Università degli Studi di Messina Dipartimento di Morfologia BiochimicaFisiologia e Produzioni AnimaliLaboratorio di Cronofisiologia VeterinariaSezione di Fisiologia VeterinariaPolo Universitario dell'Annunziata98168MessinaItaly

Objective To investigate the effects of extremely low frequency magnetic and electric fields （ELFEMFs） emitted from 380 kV transmission lines on some leukocyte differentiation antigens in dairy cows. Methods The study was carded out in 5 cows exposed to 1.98-3.28 μT of ELFEMFs and in 5 control cows exposed to 0.2-0.7 μT of ELFEMFs. Following haematological and immunologic parameters were measured in both groups： WBC, CD45R, CD6, CD4, CD8, CD21, and CD11B leukocyte antigen expression. Results Some of the haematological and immunologic parameters under investigation were similar in both groups. However, CD8 （T lymphocyte surface antigen） was higher in the exposed group （1.35 ±0.120 vs 0.50 ±0.14×10^3/mL）. Furthermore, the CD4/CD8 ratio （0.84 ±0.05 and 2.19±0.16 for exposed and not exposed cows respectively） and circadian rhythm were different between the two groups. Conclusion Exposure to ELFEMFs is responsible of the abnormal temporal variations and distribution of some haematological and immunological parameters in dairy cows.

关键词： Electro-magnetic fields Low frequency Leukocyte Circadian rhythm Dairy cow

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Case Report: Caseous Calcification of the Mitral Annulus in an 88-Year-Old Woman

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Open Journal of Internal Medicine 2020年第4期10卷 337-341页

作者： giuseppe Fede giuseppe Abate Paola Belluardo Carmelo Di Tommasi Maria Luisa Guarrella Nicoletta Guccione Guglielmo piccione Salvatore Solarino giuseppe Sulsenti Giovanni Tasca Sabina Ficili Department of Cardiovascular Disease Maggiore Hospital Modica Italy

The caseous calcification of the mitral annulus (CCMA) is a rare variant of the mitral annulus calcification (MAC), with prevalence of 0.067% in general population. It is usually an asymptomatic condition but rarely it can cause severe mitral valve dysfunction, embolization, and conduction abnormalities. Transthoracic echocardiography (TTE) is a useful diagnostic tool. However, it can be necessary a multi-modality imaging approach for a differential diagnosis among other intra-cardiac masses. We are presenting a case of CCMA in an 88-year-old woman admitted to our department for acute decompensation of chronic heart failure. TTE has shown pathognomonic findings of CCMA. Tridimensional trans-esophageal echocardiography (3D TEE) gave a better characterization of the CCMA, avoiding further diagnostic procedures.

关键词： Mitral Valve Caseous Calcification Tridimensional Echocardiography

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Adenocarcinoma of the third duodenal portion:Case report and review of literature

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World Journal of Gastrointestinal Surgery 2012年第1期4卷 23-26页

作者： Federico Sista giuseppe De Santis Antonio Giuliani Emanuela Marina Cecilia Federica piccione Laura Lancione Sergio Leardi Gianfranco Amicucci General Surgery Department of SurgeryUniversity of L’Aquila

We focus on the diagnostic and therapeutic problems of duodenal adenocarcinoma, reporting a case and reviewing the literature. A 65-year old man with adenocarcinoma in the third duodenal portion was successfully treated with a segmental resection of the third part of the duodenum, avoiding a duodeno-cephalo-pancreatectomy. This tumor is very rare and frequently affects the III and IV duodenal portion. A precocious diagnosis and the exact localization of this neoplasia are crucial factors in order to decide the surgical strategy. Given a non-specificity of symptoms, endoscopy with biopsy is the diagnostic gold standard. Duodeno-cephalo-pancreatectomy (DCP) and segmental resection of the duodenum (SRD) are the two surgical options, with overlapping morbidity (27% vs 18%) and post operative mortality (3% vs 1%). The average incidence of postoperative long-term survival is 100%, 73.3% and 31.6% of cases after 1, 3 and 5 years from surgery, respectively. Long-term survival is made worse by two factors: the presence of metastatic lymph nodes and tumor localization in the proximal duodenum. The two surgical options are radical: DCP should be used only for proximal localizations while SRD should be chosen for distal localizations.

关键词： Duodenal carcinoma Duodeno-cephalo-pancreatectomy Segmental resection Survival III duodenal portion Duodenal embryological development

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Systemic inflammation and immune response after laparotomy vs laparoscopy in patients with acute cholecystitis, complicated by peritonitis

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World Journal of Gastrointestinal Surgery 2013年第4期5卷 73-82页

作者： Federico Sista Mario Schietroma giuseppe De Santis Antonella Mattei Emanuela Marina Cecilia Federica piccione Sergio Leardi Francesco Carlei Gianfranco Amicucci Department of Surgery University of L'Aquila Department of Medicine Health and Environment Sciences University of L'Aquila

AIM: To evaluate acute cholecystitis, complicated by peritonitis, acute phase response and immunological status in patients treated by laparoscopic or open approach. METHODS: From January 2002 to May 2012, we conducted a prospective randomized study on 45 consecutive patients (27 women, 18 men; mean age 58 years). These subjects were taken from a total of 681 patients who were hospitalised presenting similar preoperative findings: acute upper abdominal pain with tenderness, involuntary guarding under the right hypochondrium and/or in the flank; fever higher than 38 ℃, leukocytosis greater than 10 × 10 9 /L or both, and ultrasonographic evidence of calculous cholecystitis possibly complicated by peritonitis. These patients had undergone cholecystectomy for acute calculous cholecystitis,complicated by bile peritonitis. Randomly, 23 patients were assigned to laparoscopic cholecystectomy (LC), and 22 patients to open cholecystectomy (OC). Blood samples were collected from all patients before operation and at days 1, 3 and 6 after surgery. Serum bacteraemia, endotoxaemia, white blood cells (WBCs), WBC subpopulations, human leukocyte antigen-DR (HLA-DR), neutrophil elastase, interleukin-1 (IL-1) and IL-6, and C-reactive protein (CRP) were measured at 0, 30, 60, 90, 120 and 180 min, at 4, 6, 12, 24 h, and then daily (8 A.M.) until post-operative day ***: The two groups were comparable in the severity of peritoneal contamination as indicated by the viable bacterial count (open group = 90% of positive cultures vs laparoscopic group = 87%) and endotoxin level (open group = 33.21 ± 6.32 pg/mL vs laparoscopic group = 35.02 ± 7.23 pg/mL). Four subjects in the OC group (18.1%) and 1 subject (4.3%) in the LC group (P < 0.05) developed intra-abdominal abscess. Severe leukocytosis (range 15.8-19.6/mL) was observed only after OC but not after LC, mostly due to an increase in neutrophils (days 1 and 3, P < 0.05). This value returned to the normal range within 3-4 d after LC and

关键词： Systemic inflammation Immune response Laparoscopy Cholecystectomy Bile peritonitis

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Case Report: Caseous Calcification of the Mitral Annulus in an 88-Year-Old Woman

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Open Journal of Internal Medicine（内科学期刊） 2020年第4期

作者： giuseppe Fede giuseppe Abate Paola Belluardo Carmelo Di Tommasi Maria Luisa Guarrella Nicoletta Guccione Guglielmo piccione Salvatore Solarino giuseppe Sulsenti Giovanni Tasca Sabina Ficili

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