检索结果-南通市图书馆

Fast separation of intact proteins with high resolution under high sample loading using non-porous RPLC packings

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2010年第16期55卷 1604-1607页

作者： WANG Fei geng xindu Institute of Modern Separation Science Shaanxi Provincial Key Laboratory of Modern Separation Science Northwest University Xi'an 710069China

A new approach for separation of intact proteins with high resolution, high speed and high sample loading ("three highs") by non-porous packings in high performance liquid chromatography is presented.A chromatographic cake having larger diameter than its thickness is firstly employed to completely separate 1.0 and 40 μg of the mixture of 7 standard proteins within 1 min un-der conventional chromatographic ***, 0.5 mg of the mixture was almost completely separated within 1 min, accom-plishing the "three high" *** research expands the application utilized for separation of intact proteins with non-porous packings.A smaller geometric size of chromatographic cake packed with much smaller particles of non-porous packings may improve results.

关键词：高效液相色谱法蛋白质分离色谱填料快速分离高负荷采样分辨率反相高分辨率

Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1999年第9期44卷 826-831页

作者： Langchong He Guangde Yang xindu geng NW Univ Xian Inst Modern Separat Sci Xian 710069 Peoples R China Xian Med Univ Coll Pharm Xian 710061 Peoples R China

A new cell membrane preparation, silica carrier cell membrane (CCM), has been presented, in which cell membrane was immobilized on silica which acted as carrier by the adsorption. The surface characteristics of the silica CCM have been tested by a scanning electron microscope and surface energy spectrometer. Comparison has been made of the changeable rule of enzymatic activity of rabbit red cell membrane in three states: the silica CCM, the suspension and the precipitation cell membranes, with temperature and time. The result showed that the silica CCM still keeps the same enzymatic activity as the other two states of cell membranes. The interactions between calcium antagonists and rabbit red and cardiac muscle cell membranes, and the stereoscopic interactions between dihydropridines and rabbit cerebellar cell membrane have been investigated by the chromatographic system of the silica CCM as a stationary phase. From the foregoing results, the chromatographic parameters obtained from the system would

关键词： cell membrane preparation silica CCM enzymatic activity affinity chromatography enantiomers.

Effect of ligand structure of stationary phase of high performance hydrophobic interaction chromatography on renaturation efficiency of GuHCl-denaturedα-chymotrypsin

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2005年第Z1期48卷 33-36页

作者： SHEN Yehua WANG Haibo BAI Quan geng xindu Institute of Modern Separation Science Shaanxi Key Laboratory of Modern Separation ScienceNorthwest UniversityXi'an 710069China

The renaturation of the denaturedα-chymotrypsin(α-Chy)with 1.7 mol·L^(-1)guanidine hydrochloride(GuHCI)by three kinds of stationary phase of high performance hydrophobic interaction chromatography(STHIC)with a comparable hydrophobicity but different ligand structures was *** obtained result indicates that the ligand structures of the three STHIC contribute to the renaturation efficiency ofα-Chy in the order of the end ligands PEG-600

关键词： protein refolding renaturation α-chymotrypsin high performance hydrophobic interaction chromatography stationary phase

Recent developments of the stoichiometric displacement model for separation processes

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2001年第21期46卷 1763-1772页

作者： geng xindu Fred E.Regnier WANG Yan Institute of Modern Separation Science Northwest Universitythe Key Lab of Modern Separation Science of Shaanxi ProvinceXi’an 710069China Department of Chemistry Purdue UniversityWest LafayetteIN 4 7907USA

Substantial attention has been paid to the stoi-chiometric displacement models for solute retention (SDM-R) in liquid chromatography and adsorption (SDM-A) at surfaces since they were developed. The SDM has a strongly theoretical basis and has evolved to the point that it is widely applied in chemistry, biochemistry, molecular biology and biotechnology. This review introduces the history, recent developments, and new concepts relating to the SDM, including theory, mathematical expressions, and applications across a broad range of fields.

关键词： liquid chromatography liquid-solid system stoichiomet- ric displacement retention model adsorption mechanism protein folding.

Studies on the refolding of the reduced-denatured insulin with size exclusion chromatography

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2005年第Z1期48卷 55-59页

作者： BAI Quan KONG Yu DONG Cuihua geng xindu Institute of Modern Separation Science Shaanxi Key Laboratory of Modern Separation ScienceNorthwest UniversityXi'an 710069China

The refolding of the reduced-denatured insulin from bovine pancreas was investigated with the size exclusion chromatography(SEC).It was shown that the reduced-denatured insulin originally denatured with 7.0 mol·L-1 guanidine hydrochloride(GuHCl)or 8.0 mol·L-1 urea could not be refolded with a non-oxidized mobile *** the oxidized and reduced glutathione(GSSG and GSH)were employed in the oxidized mobile phase,the reduced-denatured insulin still could not be ***,in the presence of 2.0 mol·L-1 urea in the oxidized mobile phase employed,the reduced-denatured insulin can be refolded with SEC,and the aggregation of denatured insulin can be diminished by *** addition,the disulfide exchange of reduced-denatured insulin also can be accelerated with GSSG/GSH in the oxidized mobile *** three disulfide bridges of insulin were formed correctly and the reduced-unfolded insulin can be renatured *** results were further tested with reversed-phase liquid chromatography(RPLC)and hydrophobic interaction chromatography(HIC).

关键词： protein folding size exclusion chromatography reduced-denaturation disulfide bonding insulin

Determination of free energy of protein folding on liquid-solid interface

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2000年第3期45卷 237-241页

作者： xindu geng Jing Zhang Yinmao Wei NW Univ Xian Inst Modern Separat Sci Prov Key Lab Modern Separat Sci Xian 710069 Peoples R China

Based on the fact that the stoichiometric displacement model for retention of solute and the total adsorption free energy of solute on a solid surface can be divided into two components, net adsorption and net desorbed energies, a new principle and an equation for calculating the free energy of protein folding, △△GF, on the solid surface are proposed. With high-performance hydrophobic interaction chromatography (HPHIC), an experimental method for determining the △△GF is established. Lysozyme and a-amylase have been selected as examples to test the new method, and their △△GF on the HPHIC stationary phase surface are found to be much higher than that reported from a solution. In addition, the △△GF of the two proteins are found to increase with the concentration of denaturing agent employed. The average standard deviations,±4.7% for lysozyme and ± 3.0% for a-amylase, indicate that the new method has a satisfactory reproducibility and reliability.

关键词： stoichiometric displacement model high-performance hydrophobic interaction chromatography adsorption free energy protein folding.

反相液相色谱中生物大分子保留自由能及相比的测定

在线全文

学校读者我要写书评

暂无评论

化学学报 2002年第5期60卷 870-875页

作者：白泉耿信笃西北大学现代分离科学研究所现代分离科学陕西省重点实验室西安710069

依据液相色谱中溶质的计量置换保留模型 (SDM R)和反相液相色谱 (RPLC)中小分子溶质柱相比的热力学新定义 ,建立了准确测定生物大分子柱相比的理论模式和实验方法 ,准确测定了生物大分子的柱相比 .同时依据计量置换参数logI和Z具有热力... 详细信息

依据液相色谱中溶质的计量置换保留模型 (SDM R)和反相液相色谱 (RPLC)中小分子溶质柱相比的热力学新定义 ,建立了准确测定生物大分子柱相比的理论模式和实验方法 ,准确测定了生物大分子的柱相比 .同时依据计量置换参数logI和Z具有热力学平衡常数的性质 ,并以logI和Z对 1/T作图呈线性为途径 ,解决了生物大分子色谱热力学研究中存在的三个难题 ,准确测定了七种标准蛋白质在RPLC保留过程中的自由能变。

关键词：反相液相色谱热力学自由能柱相比生物大分子计量置换保留模型

卵清蛋白在疏水连续棒状色谱中吸附量与溶剂浓度间关系的研究

在线全文

学校读者我要写书评

暂无评论

离子交换与吸附 2001年第3期17卷 248-255页

作者：席琛王彦耿信笃西安 710069 西北大学现代分离科学研究所!陕西省现代分离科学重点实验室

以推导改进的Langmuir模型过程中的总热力学平衡常数的表达式为基础，考察了卵清蛋白在疏水连续棒状色谱柱上的吸附行为，发现当蛋白在保留情况下，能很好地符合Langmu ir模型。而且，进一步研究了吸附量与溶剂浓度的关系，发现在保留... 详细信息

以推导改进的Langmuir模型过程中的总热力学平衡常数的表达式为基础，考察了卵清蛋白在疏水连续棒状色谱柱上的吸附行为，发现当蛋白在保留情况下，能很好地符合Langmu ir模型。而且，进一步研究了吸附量与溶剂浓度的关系，发现在保留情况下，其吸附量的对数与流动相中置换剂的摩尔浓度之间成线性关系。当流动相中硫酸铵浓度仅相差0.5mol/L时，其最大吸附量相差30倍。说明在比较柱子的吸附量时，应选择适合的溶剂浓度。提出了在等浓度条件下洗脱条件蛋白能够在色谱柱保留时的饱和吸附量为其有效吸附量，在这种条件下的流动相的组成，才是研究溶质饱和吸附量的合适组成。

关键词：液相色谱吸附量溶剂浓度疏水连续棒状色谱

液—固吸附体系中扩展的Langmuir方程的推导和检验

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

化学学报 2001年第11期59卷 1847-1852页

作者：耿信笃王彦虞启明西北大学现代分离科学研究所

从气-固吸附体系中推导出的Langmuir方程,近一世纪来只能经验性地描述液相吸附.本研究以液-固界面上的溶质计量置换模型为基础,考虑到液-固吸附体系中各组分之间的相互作用,从理论上推导出了在液-固体系中描述在不同溶剂浓度条件下的溶... 详细信息

从气-固吸附体系中推导出的Langmuir方程,近一世纪来只能经验性地描述液相吸附.本研究以液-固界面上的溶质计量置换模型为基础,考虑到液-固吸附体系中各组分之间的相互作用,从理论上推导出了在液-固体系中描述在不同溶剂浓度条件下的溶质吸附的扩展的Langmuir公式，并称其为扩展的LLangmuir公式。将Langmuir公式中经验参数与液相色谱中的计量置换平衡中的参数相关联，还将其扩展到在不同溶剂浓度条件下的溶质定量吸附的描述，为Langmuir方程在描述不同溶剂浓度条件下的组分吸附奠定了理论基础，扩大了Langmuir公式的应用。以不同溶剂浓度条件下所得到的吸附等温线数据对理论推导出的扩展的Langmuir公式进行了验证，并与计量置换平衡中的参数相关联，表明用吸附等温线法计算的计量置换参数Z与用高效液相色谱法得到的Z值符合程度很好。

关键词：液-固界面吸附机理 Langmuir模型计量置换模型吸附等温线液固吸附体系