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Solvent Effects on the Ultrastructure and Chemical Composition of Cuticular Wax and Its Potential Bioactive Role Against Alternaria alternata in Pingguoli Pear

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Journal of Integrative Agriculture 2014年第5期13卷 1137-1145页

作者： CHEN Song-jiang LI yong-cai BI Yang YIN Yan ge yong-hong WANG Yi College of Food Science and Engineering Gansu Agricultural University

Effects of different polarity solvents on the ultrastructure and chemical composition of cuticular wax in Pingguoli pear as well as their bioactive role against Alternaria alternate were studied and the results showed that the highest wax content was extracted with chloroform, and its wax content was up to 322.2 gg cm2. Long-chain fatty acids predominated in menthol extracts and n-alkanes were predominant in wax extracted with ether, chloroform and n-hexane. Pingguoli pear fruit surface was covered by a smooth and amorphous wax layer with small, scattered crystal. The morphology of recrystallized wax in vitro after removal with different solvents was not similar to that of the intact fruit surface. Removal of cuticular wax with various solvents significantly enhanced A. alternata infection, except for wax removed by methanol. The solvent extracts of methanol and chloroform stimulated the spore germination and mycelium growth ofA. alternata, but the ether and n-hexane extracts showed antifungal activity.

关键词： fruit cuticular membrane Alternaria alternata antifungal or stimulus activity

Effects of thiamine on Trichothecium and Alternaria rots of muskmelon fruit and the possible mechanisms involved

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Journal of Integrative Agriculture 2017年第11期16卷 2623-2631页

作者： ge yong-hong LI Can-ying Lü Jing-yi ZHU Dan-shi College of Food Science and Engineering Bohai University/Food Safety Key Laboratory of Liaoning Province~National & LocalJoint Engineering Research Center of Storage Processing and Safety Control Technology for Fresh Agricultural and AquaticProducts Jinzhou 121013 P.R. China

The effects of thiamine against pink and black spot rots caused by Trichothecium roseum and Alternaria alternata and modulation on the metabolism of reactive oxygen species （ROS） and phenylpropanoid pathway were investigated in this paper. In vitro test indicated that thiamine significantly inhibited mycelia growth and spore germination of T. roseum and A. alternata. Thiamine at 100 mmol L-1 effectively inhibited lesion development of muskmelon fruit inoculated with T. roseum or A. alternata, enhanced production rate of O2; and H2O2 content, activities of catalase （CAT）, ascorbate peroxidase （APX） and glutathione reductase （GR） in muskmelon fruit. Thiamine also affect phenylpropanoid pathway in muskmelon fruit by increasing the activities of phenylalanine ammonia lyase （PAL） and peroxidase （POD）, the content of total phenolic compounds, flavonoids and lignin. These results suggest that the effects of thiamine on pink and black spot rots in muskmelon fruits are associated with its direct fungitoxic against the pathogens and the modulation of O2- and H2O2 production, elimi- nating enzymes and phenylpropanoid pathway.

关键词： induced resistance thiamine muskmelon fruit antimicrobial activity phenylpropanoid pathway

Induces Resistance Against Fusarium and Pink Rots by Acibenzolar-S-Methyl in Harvested Muskmelon (***)

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Agricultural Sciences in China 2008年第1期7卷 58-64页

作者： ge yong-hong BI Yang LI Xuan LI Mei College of Food Science and Engineering Gansu Agricultural University Lanzhou 730070 P.R.China

Acibenzolar-S-methyl （ASM） is a chemical activator of systematic resistance in many plants. The effect of preharvest and postharvest application of ASM was evaluated for its ability to induce resistance in muskmelon fruit. The results indicated that 50 and 100 mg L^-1 ASM or 1 mL L^-1 imazalil at 1 week or 1 day before harvest were effective in reducing the lesion area with 100 mg L^-1 ASM the most effective. No treatment inhibited the infection rate. The postharvest results showed that 50 and 100 mg L^-1 ASM, and 0.1 mL L^-1 imazalil were effective in reducing the lesion area with 100 mg L^-1 ASM the most effective. No treatment inhibited the infection rate. There was a clear time-dependent response of the fruit to postharvest ASM treatment, in which treatments applied 1, 3, and 5 day before inoculation provided the best results. ASM did not demonstrate any fungicide effect in vitro and suppressed lesion area in treated muskmelons, indicating that disease resistance was induced. The protection of ASM was associated with the activation of peroxidase （POD） in treated muskmelons.

关键词： acibenzolar-S-methyl (ASM) induced resistance muskmelon peroxidase (POD)

Application of methyl jasmonate postharvest maintains the quality of Nanguo pears by regulating mitochondrial energy metabolism

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Journal of Integrative Agriculture 2021年第11期20卷 3075-3083页

作者： LI Can-ying CHENG Yuan HOU Jia-bao ZHU Jie SUN Lei ge yong-hong College of Food Science and Technology/National and Local Joint Engineering Research Center of Storage Processing and Safety Control Technology for Fresh Agricultural and Aquatic ProductsBohai UniversityJinzhou 121013P.R.China

The present study was conducted to investigate the effects of methyl jasmonate(Me JA)dipping treatment on mitochondrial energy metabolism and quality parameters of Nanguo pears during room temperature *** results showed that Me JA treatment suppressed the respiration rate and weight loss,and maintained the flesh firmness of Nanguo *** JA also effectively maintained the content of ascorbic acid and titratable acidity in the ***,the activities of H^(+)-ATPase,Ca^(2+)-ATPase,succinate dehydrogenase(SDH)and cytochrome C oxidase(CCO)of the Me JA-treated fruit were significantly higher than those of the untreated *** contents of adenosine triphosphate(ATP)and adenosine diphosphate(ADP)and the energy charge were also enhanced by Me JA *** results suggest that postharvest Me JA treatment could maintain the quality of Nanguo pears,in part by modulating mitochondrial energy metabolism during room temperature storage.

关键词： Pyrus ussuriensis energy metabolism methyl jasmonate respiration rate

Postharvest Chitosan Treatment Induces Resistance in Potato Against Fusarium sulphureum

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Agricultural Sciences in China 2008年第5期7卷 615-621页

作者： SUN Xiao-juan BI Yang LI yong-cai HAN Rui-feng ge yong-hong College of Food Science and Engineering Gansu Agricultural University Lanzhou 730070 P.R.China College of Agronomy Gansu Agricultural University Lanzhou 730070 P.R.China

The effects of chitosan treatment and inoculation on dry rot in tubers and slices of potato were studied. The results showed that chitosan treatment significantly reduced the lesion diameter of potato inoculated with Fusarium sulphureum. The treatment at 0.25% showed the best effect. Chitosan at 0.25% increased the activities of peroxidase and polyphenoloxidase, and the contents of flavonoid compounds and lignin in tissues. Increased activities of 13-1,3-glucanase, and phenylalanine ammonialyase were observed, but there were no significant differences between the treated and the control. These findings suggested that the effects of chitosan could be associated with the induced resistance against Fusarium dry rot in potato.

关键词： chitosan potato decay induced resistance

Postharvest ASM or Harpin Treatment Induce Resistance of Muskmelons Against Trichothecium roseum

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Agricultural Sciences in China 2008年第2期7卷 217-223页

作者： WANG Yi LI Xuan BI Yang ge yong-hong LI yong-cai XIE Fang College of Food Science and Engineering Gansu Agricultural Unversity Lanzhou 730070 P.R.China College of Chemical and Biological Engineering Lanzhou Jiaotong University Lanzhou 730070 P.R.China

Induced resistance was studied in muskmelons （cv. Yindi） inoculated Trichothecium roseum with postharvest 1,2,3- benzothiadiazole-7-carbothioic acid S-methyl ester （ASM）（100 mg L^-1） or harpin （50 mg L^-1） treatment. Both ASM and harpin significantly reduced lesion diameter in inoculated fruit. Lesion diameter was limited in the treated and untreated halves of the same fruit, indicating that the local and systemic resistance was induced. Inhibiting efficacy of elicitors lasted 7 and 5 days in the treated and untreated halves. The resistance increased by the chemicals was associated with the activation of peroxidase （POD） and chitinase （CHT）. The elicitors induced a significantly and progressively increasing activity of POD and CHT in the treated and untreated halves, and the activation lasted at least 5 days. The activities of POD isoenzymes increased in the treated fruit. However, no new enzyme band was found in the treated and untreated halves.

关键词： muskmelon elicitor decay induced resistance

Control of Postharvest Fusarium Rot and Trichothecium Rot in Harvested Muskmelon (cv. Yindi) by Harpin

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Agricultural Sciences in China 2005年第12期4卷 943-948页

作者： ge yong-hong BI Yang LI Mei LI Xuan College of Food Science and Engineering Gansu Agricultural University Lanzhou 730070 P.R.China College of Agronomy Gansu Agricultural University Lanzhou 730070 P.R.China

Preharvest and postharvest application of Harpin was evaluated for its ability to induce resistance in muskmelon fruit and control rots. Preharvest treatments were 30 mg L^-1, 60 mg L^-1 Harpin or 1 mg L^-1 Imazalil either I week or I day before harvest. The CK was untreated. Fruit were then inoculated with Fusarium semitectum or Trichothecium roseum 48 h after harvest, and stored at 23± 1℃, RH （relative humidity） 50-60% for 4 d. All treatments were effective in reducing the lesion area with 60 mg L^-1 Harpin the most effectively. No treatments inhibited infection rate. Postharvest treatment was fruit dips of 30 mg L i, 60 mg L^-1 Harpin, 0.1 mg L i Imazalil. Fruit were inoculated with F. semitectum or T. roseum 24, 72, 120 or 168 h after treatment, and stored at 23 ± 1℃, RH 50-60% for 4 days. All treatments were effective in reducing the lesion area with 60 mg L^-1 Harpin the most effectively. No treatments inhibited the infection rate. Postharvest Harpin treatment induced the peroxidase activity increase, peroxidase activity reached maximum after 8 d and the activation lasted at least 10 d.

关键词： Harpin Muskmelon Fusarium semitectum Trichothecium roseum Peroxidase

基于OMRON PLC与HMI的青藏铁路客车电气控制系统

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机车电传动 2009年第3期 20-23,41页

作者：葛洪勇北车青岛四方车辆研究所有限公司山东青岛266031

阐述了以OMRON的CPM2A系列PLC及其扩展模块、NT系列可编程终端HMI为控制核心的青藏铁路客车电气控制系统,详细分析了OMRON自动化产品在青藏铁路客车电气控制屏柜中的设计原理、编程与调试技巧、故障处理方法等典型应用。

关键词：青藏铁路电气控制低压电气屏柜 PLC HMI触摸屏

新型城镇化视角下智慧旅游城市建设模式研究——基于河北省智慧旅游城市建设分析

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河北学刊 2016年第3期36卷 197-202页

作者：王红宝葛永红河北地质大学商学院河北石家庄050031

随着中国新型城镇化建设的加速,旅游业的迅猛发展,现代信息化技术的不断更新,智慧旅游城市建设逐步成为旅游城市转型升级的新途径。河北省城市旅游资源丰富,建设智慧旅游城市优势明显。本文在智慧旅游城市相关理论的基础上,分析了河北... 详细信息

随着中国新型城镇化建设的加速,旅游业的迅猛发展,现代信息化技术的不断更新,智慧旅游城市建设逐步成为旅游城市转型升级的新途径。河北省城市旅游资源丰富,建设智慧旅游城市优势明显。本文在智慧旅游城市相关理论的基础上,分析了河北省智慧旅游城市建设现状,明确了构建智慧旅游城市的前提条件,并从目标模式和实施模式两个角度对智慧旅游城市建设模式进行了构建,可对河北省乃至全国智慧旅游城市建设提供重要启示意义。

关键词：智慧旅游城市建设模式新型城镇化物联网数据平台