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generation and Analysis of Expressed Sequence Tags(ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

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Journal of Integrative Agriculture 2014年第2期13卷 378-386页

作者： ZHAO Su-mei LIU Yong-gang PAN Hong-bing ZHANG Xi ge chang-rong JIA Jun-jing GAO Shi-zheng Yunnan Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University

Porcine skeletal muscle genes play a major role in determining muscle growth and meat quality. Construction of a full-length cDNA library is an effective way to understand the expression of functional genes in muscle tissues. In addition, novel genes for further research could be identified in the library. In this study, we constructed a full-length cDNA library from porcine muscle tissue. The estimated average size of the cDNA inserts was 1 076 bp, and the cDNA fullness ratio was 86.2%. A total of 1 058 unique sequences with 342 contigs （32.3%） and 716 singleton （67.7%） expressed sequence tags （EST） were obtained by clustering and assembling. Meanwhile, 826 （78.1%） ESTs were categorized as known genes, and 232 （21.9%） ESTs were categorized as unknown genes. 65 novel porcine genes that exhibit no identity in the TIGR gene index of Sus scrofa and 124 full-length sequences with unknown functions were deposited in the dbEST division of genBank （accession numbers： EU650784-EU650788, ge843306, GH228978-GH229100）. The abundantly expressed genes in porcine muscle tissue were related to muscle fiber development, energy metabolism and protein synthesis. gene ontology analysis showed that sequences expressed in porcine muscle tissue contained a high percentage of binding activity, catalytic activity, structural molecule activity and motor activity, which involved mainly in metabolic, cellular and developmental process, distributed mainly in intracellular region. The sequence data generated in this study would provide valuable information for identifying porcine genes expressed in muscle tissue and help to advance the study on the structure and function of genes in pigs.

关键词： muscle tissue full-length cDNA library expressed sequence tag pig

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云南盐津乌骨鸡肉中鲜味肽的提取与鉴定

中国调味品 2024年第5期49卷 10-15,46页

作者：杨超辉梁双敏葛长荣肖智超云南农业大学食品科学技术学院昆明650201 云南农业大学云南省畜产品加工工程技术研究中心昆明650201 云南农业大学昆明650201

利用超滤和凝胶过滤色谱法对盐津乌骨鸡肉中的鲜味肽进行分离纯化,结合感官评价得到鲜味最佳的组分F1,进一步采用液相色谱-串联质谱联用技术从F1组分中鉴定出鲜味肽,对鉴定出的鲜味肽进行条件筛选、氨基酸组成和活性片段预测分析。结果... 详细信息

利用超滤和凝胶过滤色谱法对盐津乌骨鸡肉中的鲜味肽进行分离纯化,结合感官评价得到鲜味最佳的组分F1,进一步采用液相色谱-串联质谱联用技术从F1组分中鉴定出鲜味肽,对鉴定出的鲜味肽进行条件筛选、氨基酸组成和活性片段预测分析。结果表明,从盐津乌骨鸡肉中鉴定出921条多肽,筛选后得到551条多肽,通过鲜味活性预测得到鲜味片段出现频率大于1的10条多肽,其中DTEEVEHgeE、EVHEEEVH、HEAEEVHEE、HEKLSEESEE、KVED、TPIPDLP 6条多肽可能具有较强的鲜味。

关键词：盐津乌骨鸡鲜味肽分离鉴定条件筛选活性预测

Muscle Biological Characteristics of Differentially Expressed genes in Wujin and Landrace Pigs

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Journal of Integrative Agriculture 2014年第10期13卷 2236-2242页

作者： XU Hong HUANG Ying LI Wei-zhen YANG Ming-hua ge chang-rong ZHANG Xi LI Liu-an GAO Shi-zheng ZHAO Su-mei School of Public Finance and Economics Yunnan University of Finance and Economics Yunnan Key Laboratory of Animal Nutrition and Feed Science Yunnan Agricultural University Faculty of Animal Science and Technology Yunnan Agricultural University Animal Husbandry Post-Doctoral Station Nanjing Agricultural University Department of Animal Science Tianjin Agricultural University

The biological chemistry would be responsible for the meat quality. This study tried to investigate the transcript expression profile and explain the characteristics of differentially expressed genes between the Wujin and Landrace pigs. The results showed that 526 differentially expressed genes were found by comparing the transcript expression profile of muscle tissue between Wujin and Landrace pigs. Among them, 335 genes showed up-regulations and 191 genes showed down-regulations in Wujin pigs compared with the Landrace pigs. gene ontology （GO） analysis indicated that the differentially expressed genes were clustered into three groups involving in protein synthesis, energy metabolism and immune response. Kyoto encyclopedia of genes and genomes （KEGG） pathway analysis found that these differentially expressed genes participated in protein synthesis metabolism, energy metabolism and immune response pathway. The Database for Annotation, Visualization and Integrated Discovery （DAVID） analysis of protein function and protein domains function also confirmed that differentially expressed genes belonged to protein synthesis, energy metabolism and immune response. genes related protein synthesis metabolism pathway in Landrace was higher than in Wujin pigs. However, differentially expressed genes related energy metabolism and immune response was up-regulated in Wujin pigs compared with Landrace pigs. Quantitative real-time RT-PCR on selected genes was used to confirm the results from the microarray. These suggested that the genes related to protein synthesis, energy metabolism and immune response would contribute to the growth performance, meat quality as well as anti-disease capacity.

关键词： pigs muscle tissue microarray bioinformatics analysis differentially expressed genes

Effects of the Sheep Polyclonal Antibodies Against the Porcine Adipocyte Plasma Membrane Proteins on Porcine Carcass Composition and Meat Quality

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Agricultural Sciences in China 2007年第10期6卷 1256-1261页

作者： GAO Shi-zheng HU Hong-mei LIU Ling-yun ZHANG Xi LIU Yong-gang ge chang-rong Key Laboratory of Animal Nutrition and Feed of Yunnan Province Yunnan Agricultural University Kunming 650201 P.R.China Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences Jinan 250100 P.R.China Kunming Normal School Kunming 650201 P.R.China

To detect the effects of the polyclonal antibodies raised in sheep against porcine adipocyte plasma membranes on the porcine carcass composition and meat quality, 30 pigs assigned into 6 treatment groups were given intraperitoneal injections of sheep antipig adipocyte plasma membrane immunoglobulin （ASIg） or sheep nonimmune serum immunoglobulin （NSIg）. At the end of the experiment, the pigs were slaughtered at 90 kg body weight, and carcasses and meat quality were evaluated. The results showed that when pigs intraperitoneally immunized with 20 or 30 mg ASIg at 15 kg body weight, 20 mg purified ASIg twice at 15 and 60 kg body weight, or 20 mg purified ASIg at 60 kg body weight, respectively, their lean meat percentage, fat meat percentage, backfat thickness, loin eye area leaf fat weight, caul fat weight, heart weight, liver weight, and kidney weight were significantly affected. However, the kidney weight, lurrg weight, dressing percentage, and spleen weight did not remarkably change. Our results indicated that pigs intraperitoneally immunized with 20 or 30 mg ASIg at 15 kg body weight, and 20 mg ASIg twice at 15 and 60 kg body weight, have significantly different drip loss rate, cooked meat ratio, tenderness, storage loss rate, muscle fiber diameter, moisture content, dry matter content, crude protein content, and crude fat content from the control group that received 20 mg NSIg at 15 kg body weight. However, meat pH, meat color value, meat marbling score, inosinate, and myohemoglobin were not significantly affected. Our results indicated ASIg could not significantly affect the content of most muscular amino acids and intramuscular fatty acids.

关键词： polyclonal antibodies pig carcass composition meat quality

Effects of Monoclonal Antibody Against Adipocyte-Specific Membrane Protein on Lipid Metabolism in Pigs

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Agricultural Sciences in China 2008年第2期7卷 232-238页

作者： GAO Shi-zheng LIU Ling-yun ZHAO Su-mei HU Hong-mei ge chang-rong LIU Yong-gang ZHANG Xi Key Laboratory of Animal Nutrition and Feed of Yunnan Province Yunnan Agricultural University Kunming 650201 P.R.China Kunming Normal School Kunming 650201 P.R.China Institute of Zootechnics and Veterinary Science Shandong Academy of Agricultural Sciences Jinan 250100 P.R.China

This study was to investigate the regulation of monoclonal antibodies against adipocyte membrane proteins （McAb） on lipid metabolism in pigs. Forty Landrace x Saba pigs were randomly divided into eight groups; the control group was given 10 mL saline and the treat groups were given monoclonal antibody against adipocyte-specific membrane protein with 0.1, 0.5, and 1.0 mg kg-I body weight at 15 and 60 kg body weight, respectively, by intraperitoneal injection. The results showed that McAb could increase, significantly, serum lipoprotein lipase activity and reduce serum nonesterified fatty acid （NEFA） content. Meanwhile, McAb increased content of serum lipid, triglyceride （TG）, cholesterol （CHO）, high density lipoprotein （HDL）, and low density lipoprotein （LDL） both at 15 and 60 kg body weight. However, McAb affected more significantly the lipid metabolism at 15 kg body weight than at 60 kg body weight. Moreover, this effect of McAb on lipid metabolism exhibited dose-dependent effect. These results suggested that this monoclonal antibody increased lipase activity, promoted lipolysis, and utilization of lipid so that McAb could be applied to restrain excessive fat deposition in porcine production through the regulation of fat metabolism.

关键词： adipocyte membrane protein monoclonal antibody lipid metabolism porcine

Effect of Crossbreed on the Muscle Quality(Chemical Composition) in Yun-Ling Black Goats

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Agricultural Sciences in China 2009年第1期8卷 108-114页

作者： JIA Jun-jing TIAN Yun-bo ZHANG Xi HUANG Qi-chao WEN Sheng-ping GU Feng-ying ge chang-rong CAO Zhen-hui CHENG Zhi-bin M. Jois Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science Yunnan Agricultural University Kunming 650201 P.R.China College of Zhongkai Agricultural Technology and Science Guangzhou 510225 P.R. China College of Food Science and Technology Yunnan Agricultural University Kunming 650201 P.R.China Department of Agriculture School of Life Sciences La Trobe University Bundoora 3086 Victoria Australia

Twenty castrated male goats, each of Yun-Ling Black goats （YLB goat）, N × YLB hybrid goats （Nubian ♂ ×Yun-Ling Black goats 9） and B × YLB hybrid goats （Boer ♂× Yun-Ling Black goats ♀）, were used to evaluate the effect of crossbreeding on the meat chemical composition in the YLB goats of China. After weaning of 90 days, all the experimental goats were reared on natural pasture when they were slaughtered at an age of 730 days. The longissimus dorsi （LD） and biceps femoris （BF） muscles were sampled from each carcass to determine chemical compositions. Both hybrid goats had higher protein content （P 〈 0.01） and lower fat content （P 〈 0.05） than the YLB goats in the two types of muscle. The inosinic acid contents of LD muscle for the YLB goats and the B × YLB hybrid goats were significantly higher （P 〈 0.05） than that for the N × YLB hybrid goats. The LD muscle from the YLB goats contained higher essential amino acid （P 〈 0.01）, total amino acid （P 〈 0.01）, and some individual amino acid （P 〈 0.05） than those from the hybrid goats. The concentration of unsaturated fatty acid （USFA） of LD muscle from the N × YLB hybrid goats was significantly higher than the other two goat breeds （P〈0.05） but did not differ between the other two goat breeds （P〉0.05）. The YLB goats had significantly higher （P〈0.05） concentrations of oleic acid （C18：1） and linolenic acid （C18：3） of LD muscle than the hybrid goats, had significantly higher （P 〈 0.05） proportion of mono-unsaturated （Sum for C 16：1 and C 18：1） than the B ×YLB hybrid goats, and tended to be higher than the N × YLB hybrid goats （P 〉 0.05）. In contrast, the proportion of poly-unsaturated in the YLB goats was significantly lower （P 〈 0.05） than that in the hybrid goats.

关键词： Yun-Ling Black goat crossbreed muscle amino acids muscle fatty acids

Two-photon polymerization-based 4D printing and its applications

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International Journal of Extreme Manufacturing 2024年第1期6卷 1-25页

作者： Bingcong Jian Honggeng Li Xiangnan He rong Wang Hui Ying Yang Qi ge Shenzhen Key Laboratory of Soft Mechanics&Smart Manufacturing Southern University of Science and TechnologyShenzhen 518055People’s Republic of China Department of Mechanical and Energy Engineering Southern University of Science and TechnologyShenzhen 518055People’s Republic of China Pillar of Engineering Product Development Singapore University of Technology and Design8 Somapah RoadSingapore 487372Singapore

Two-photon polymerization(TPP)is a cutting-edge micro/nanoscale three-dimensional(3D)printing technology based on the principle of two-photon absorption.TPP surpasses the diffraction limit in achieving feature sizes and excels in fabricating intricate 3D micro/nanostructures with exceptional resolution.The concept of 4D entails the fabrication of structures utilizing smart materials capable of undergoing shape,property,or functional changes in response to external stimuli over time.The integration of TPP and 4D printing introduces the possibility of producing responsive structures with micro/nanoscale accuracy,thereby enhancing the capabilities and potential applications of both technologies.This paper comprehensively reviews TPP-based 4D printing technology and its diverse applications.First,the working principles of TPP and its recent advancements are introduced.Second,the optional4D printing materials suitable for fabrication with TPP are discussed.Finally,this review paper highlights several noteworthy applications of TPP-based 4D printing,including domains such as biomedical microrobots,bioinspired microactuators,autonomous mobile microrobots,transformable devices and robots,as well as anti-counterfeiting microdevices.In conclusion,this paper provides valuable insights into the current status and future prospects of TPP-based4D printing technology,thereby serving as a guide for researchers and practitioners.

关键词： two-photonpolymerization 4D printing nano/micro fabrication microrobot

Enantioselective Synthesis of Benzimidazole Atropisomers Featuring a N-N Axis

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Chinese Journal of Chemistry 2024年第7期42卷 711-718页

作者： Fang-Bei ge Qi-Kun Yin Chuan-Jun Lu Xuan Xuan Jia Feng Ren-rong Liu College of Chemistry and Chemical Engineering Qingdao UniversityQingdaoShandong 266071China School of Pharmacy Key Laboratory of Mlecular Pharmacoogy and Drug EvaluationMinistry of EducationYantai Universit YantaiShandong 264005China

Atroposelective synthesis of N-N atropisomers is an emerging area but remains underexplored;in particular,the synthesis of N-N benzimidazole atropisomers is still unprecedented.Herein,the first enantioselective synthesis of N-N benzimidazole atropisomers via the palladium-catalyzed de novo construction of benzimidazole skeleton is presented.With readily available palladium catalyst and biphosphine ligand,a broad range of nonbiaryl benzimidazole and indole-benzimidazole atropisomers were conveniently ac-cessed in high yields and with excellent enantioselectivities.Significantly,these N-N benzimidazole atropisomers showed great anti-tumor activity and selectivity to breast cancer MCF-7 cells.The simple catalytic system,broad substrate scope,high enantioselectivi-ty,and good bioactivity make this approach highly attractive.

关键词： Asymmetric synthesis Atropisomerism Palladium Benzimidazole Antitumor

MXene Enhanced 3D Needled Waste Denim Felt for High‑Performance Flexible Supercapacitors

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Nano-Micro Letters 2024年第2期16卷 383-394页

作者： Wei Fan Qi Wang Kai rong Yang Shi Wanxi Peng Handong Li Zhanhu Guo Ben Bin Xu Hua Hou Hassan Algadi Shengbo ge Key Laboratory of Functional Textile Material and Product of the Ministry of Education School of Textile Science and EngineeringInstitute of Flexible electronics and Intelligent TextileXi’an Polytechnic UniversityXi’an 710048People’s Republic of China Co‑Innovation Center of Efficient Processing and Utilization of Forest Resources College of Materials Science and EngineeringNanjing Forestry UniversityNanjing 210037People’s Republic of China Henan Province International Collaboration Lab of Forest Resources Utilization School of ForestryHenan Agricultural UniversityZhengzhou 450002People’s Republic of China Integrated Composites Lab Department of Mechanical and Construction EngineeringNorthumbria UniversityNewcastle Upon Tyne NE18STUK College of Materials Science and Engineering Taiyuan University of Science and TechnologyTaiyuan 030024People’s Republic of China Department of Electrical Engineering Faculty of EngineeringNajran University11001 NajranSaudi Arabia

MXene,a transition metal carbide/nitride,has been prominent as an ideal electrochemical active material for supercapacitors.However,the low MXene load limits its practical applications.As environmental concerns and sustainable development become more widely recognized,it is necessary to explore a greener and cleaner technology to recycle textile by-products such as cotton.The present study proposes an effective 3D fabrication method that uses MXene to fabricate waste denim felt into ultralight and flexible supercapacitors through needling and carbonization.The 3D structure provided more sites for loading MXene onto Z-directional fiber bundles,resulting in more efficient ion exchange between the electrolyte and electrodes.Furthermore,the carbonization process removed the specific adverse groups in MXenes,further improving the specific capacitance,energy density,power density and electrical conductivity of supercapacitors.The electrodes achieve a maximum specific capacitance of 1748.5 mF cm-2 and demonstrate remarkable cycling stability maintaining more than 94%after 15,000 galvanostatic charge/discharge cycles.Besides,the obtained supercapacitors present a maximum specific capacitance of 577.5 mF cm^(-2),energy density of 80.2μWh cm^(-2)and power density of 3 mW cm^(-2),respectively.The resulting supercapacitors can be used to develop smart wearable power devices such as smartwatches,laying the foundation for a novel strategy of utilizing waste cotton in a high-quality manner.

关键词： MXene 3D needled waste denim felt Supercapacitors Carbonization