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Nitrate removal to its fate in wetland mesocosm filled with sponge iron:Impact of influent COD/N ratio

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Frontiers of Environmental Science & Engineering 2020年第1期14卷 77-85页

作者： Zhihao Si Xinshan Song Xin Cao Yuhui Wang Yifei Wang yufeng Zhao Xiaoyan ge Awet Arefe Tesfahunegn College of Environmental Science and Engineering State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile IndustryDonghua UniversityShanghai 201620China

Sponge iron(s-Fe°)is a porous metal with the potential to be an electron donor for *** study aims to evaluate the feasibility of using s-Fe as the substrate of wetland ***,wetland mesocosms with the addition of s-Fe"particles(CW-Fc)and a blank control group(CW-CK)were *** NOj-N reduction property and water quality parameters(*** ORP)were examined at three COD/N ratios(*** 10).Results showed that the NO3--N removal efficiencies were significantly increased by 6.6 to 58.9%in the presence of s-Fe NH4+-N was mainly produced by chemical denitrification,and approximately 50% of the NO3--N was reduced to N4+-N,at the COD ratio of *** increase of the influent COD/N ratio resulted in lower chemical denitrification and higher *** chemical denitrification mediated by s-Fe led to an accumulation of NH4+-N at COD/N ratios of 0 and *** TIN removal efficiencies increased by 4.5#12.4#.Moreover,the effluent pll,*** ORP values showed a significant negative correlation with total Fe and Fe(II)(P<0.01).High-throughput sequencing analysis indicated that Trichococctis(77.2%)was the most abundant microorganism in the CW-Fc mcsocosm,while Thauera,*** Herbaspirillum were the primary denitrifying *** denitrifiers,Simplicispira,IXxhloronwnas,and *** the dominant bacteria for *** study provides a valuable method and an improved understanding of NOj-N reduction characteristics of s-Fc in a wetland mcsocosm.

关键词： Sponge iron Wetland mesocosm Electronic donor Denitrification COD/N ratio

伴BRAF V600E基因突变的儿童后肾间质瘤1例并文献复习

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中华实用儿科临床杂志 2024年第4期39卷 306-310页

作者：毛舒婷王叨李白刘姗姗魏林林苏淑芳徐岩马亚南周歌刘玉峰郑州大学第一附属医院儿科郑州450052

对2022年6月郑州大学第一附属医院收治的1例伴BRAF V600E基因突变的儿童后肾间质瘤(MST)患儿的临床资料进行回顾性分析,并进行文献复习。患儿,女,2岁,因发现左侧腹部肿物就诊,肿瘤长径10.5 cm。行左侧肾根治性切除术,术后病理提示MST。... 详细信息

对2022年6月郑州大学第一附属医院收治的1例伴BRAF V600E基因突变的儿童后肾间质瘤(MST)患儿的临床资料进行回顾性分析,并进行文献复习。患儿,女,2岁,因发现左侧腹部肿物就诊,肿瘤长径10.5 cm。行左侧肾根治性切除术,术后病理提示MST。镜下,肿瘤缺乏包膜,膨胀性生长为主,肿瘤细胞呈梭形或星状,富细胞区核分裂易见。肿瘤内部可见发育不良的血管;血管及内陷肾小管周围肿瘤细胞呈洋葱皮样排列。免疫组织化学染色CD34阳性,荧光PCR法检测到BRAF V600E突变阳性。文献检索相关病例报道共21篇,其中英文16篇、中文5篇,结合本研究1例,共纳入58例MST患儿,年龄2 d至15岁,中位年龄为2岁。除2例性别不明外,男女比例约为1.4∶1.0。MST多数无症状,肿瘤大小平均5.3 cm。肿瘤细胞CD34呈不同程度阳性表达。8例行BRAF V600E突变检测,均为阳性。58例患儿接受肾切除术,其中7例患儿辅助放化疗,随访0~156个月,1例死亡,1例复发。MST是临床罕见的肾脏良性间质瘤,BRAF V600E突变见于多种恶性肿瘤。本研究为国内首次对MST病例进行BRAF V600E基因突变的报道,并指出了BRAF突变的分子检测对MST准确诊断的重要性。

关键词：病理后肾间质瘤儿童 BRAF V600E基因

用于快速识别红耳龟在中国野外分布的特异性引物筛选

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亚热带农业研究 2018年第2期14卷 86-90页

作者：曹代男李伟业滑留帅葛研魏玉峰段好冉龚世平广东省生物资源应用研究所/广东省动物保护与资源利用重点实验室/广东省野生动物保护与利用公共实验室广东广州510260

红耳龟(Trachemys scripta elegans)是世界上最危险的100个入侵物种之一,自20世纪80年代被引入中国,目前已在中国野外广泛分布。环境DNA技术是物种调查和监测的有力工具,为了能够利用环境DNA技术快速识别中国野外红耳龟的分布状况,筛选... 详细信息

红耳龟(Trachemys scripta elegans)是世界上最危险的100个入侵物种之一,自20世纪80年代被引入中国,目前已在中国野外广泛分布。环境DNA技术是物种调查和监测的有力工具,为了能够利用环境DNA技术快速识别中国野外红耳龟的分布状况,筛选了红耳龟线粒体COI基因的特异性引物。该引物能够有效区分红耳龟与中国野外其他常见的10种龟类,可为红耳龟的野外分布监测和防控提供参考。

关键词：红耳龟快速识别线粒体COI基因环境DNA 中国

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黄脚黄羽地方蛋鸡杂交组合性能测定

中国畜禽种业 2023年第8期19卷 4-9页

作者：葛宇峰王星果马猛王克华郭军窦套存胡玉萍曲亮江苏省家禽科学研究所江苏扬州225125

地方鸡种如皋黄鸡的C3品系拥有良好的产蛋性能,但其蛋重较小、淘汰母鸡体重较轻,因此有必要利用体型较大的地方鸡公鸡进行杂交改良。该研究以来源于鹿苑鸡的C5品系为父本,与C3品系母鸡杂交,测定后代的体重体尺、产蛋性能和蛋品质。结果... 详细信息

地方鸡种如皋黄鸡的C3品系拥有良好的产蛋性能,但其蛋重较小、淘汰母鸡体重较轻,因此有必要利用体型较大的地方鸡公鸡进行杂交改良。该研究以来源于鹿苑鸡的C5品系为父本,与C3品系母鸡杂交,测定后代的体重体尺、产蛋性能和蛋品质。结果发现,与C3相比,C5(父)×C3(母)生长发育速度快,产蛋性能低,蛋品质高。可见,C5(父)×C3(母)基本达到杂交改良的目的,后期选育中需要重点提高C5品系产蛋性能。

关键词：如皋黄鸡鹿苑鸡杂交生产性能

Cobalt doped Fe-Mn@CNTs catalysts with highly stability for lowtemperature selective catalytic reduction of NO_(x)

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Nano Research 2022年第4期15卷 3001-3009页

作者： Pengfei Li Tiezhen Zhang Haixiao Sun yufeng Gao Yanyuan Zhang Yuanyuan Liu Chengmin ge Hao Chen Xiaoping Dai Xin Zhang State Key Laboratory of Heavy Oil Processing College of Chemical EngineeringChina University of PetroleumBeijing 102249China Petrochina Daqing Petrochemical Research Center Daqing 163714China ShanDong DongYuan New Material Technology Co. LtdDongying 257300China

In this paper,we report the fabrication of cobalt-doped de-NO_(x)catalyst by pyrolyzing an analogous metal-organic framework-74(MOF-74)containing Fe&*** resulted catalyst exhibits distinctive microstructures of manganese,cobalt,and iron immobilized on N-doped carbon nanotubes(CNTs).It is found through experiments that the trimetallic catalyst Fe_(2)Mn_(1)Co_(0.5)/CNTs-50 has the best NH_(3)-selective catalytic reduction(SCR)*** Fe_(2)Mn_(1)Co_(0.5)/CNTs-50 exhibited excellent water and sulfur resistance and good stability under the harsh gas environment of 250℃ and/or 170℃,NO=NH_(3)=1,000 ppm,8 vol.%O_(2),20 vol.%H2O,1,000 ppm SO_(2),and gas hourly space velocity(GHSV)=75,000 h^(-1).The de-NO_(x)conversion was maintained about 55%and 25%after 192 *** water and sulfur resistance performance were much higher than commercial vanadium series *** highly water and sulfur resistance performance may be attributed to the unique core-shell microstructure and the synergistic effect of manganese,cobalt,and iron which helps reduce the formation for byproducts(NH_(4)HSO_(4)).This study may promote to explore the development of a high stability catalyst for low-temperature selective catalytic reduction of NO_(x)with NH_(3).

关键词： low-temperature NH_(3)-selective catalytic reduction(SCR) metal-organic framework(MOFs) water and sulfur resistance high stability

Rapid bioluminescence assay for monitoring rat CES1 activity and its alteration by traditional Chinese medicines

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Journal of Pharmaceutical Analysis 2020年第3期10卷 253-262页

作者： Jun Zhang Dandan Wang Liwei Zou Min Xiao yufeng Zhang Ziwei Li Ling Yang Guangbo ge Zhong Zuo School of Pharmacy Faculty of MedicineThe Chinese University of Hong KongHong KongChina Institute of Interdisciplinary Medicine Shanghai University of Traditional Chinese MedicineShanghai201203China

In traditional Chinese medicine herbs(TCM),including Radix Salviae Miltiorrhizae(Danshen),Radix Puerariae Lobatae(gegen),Radix Angelicae Sinensis(Danggui),and Rhizoma Chuanxiong(Chuanxiong)are widely used for the prevention and treatment of cardiovascular diseases and also often co-administered with Western drugs as a part of integrative medicine *** 1(CES1)plays a pivotal role in the metabolisms of pro-drugs,Since(S)-2-(2-(6-dimethylamino)-benzothiazole)-4,5-dihydrothiazole-4-carboxylate(NLMe)has recently been identified by us as a selective CES1 bioluminescent sensor,we developed a rapid method using this substrate for the direct measurement of CES1 activity in *** bioluminescence assay was applied to determine CES1 activity in rat tissues after a two-week oral administration of each of the four herbs noted *** results demonstrated the presence of CES1 enzyme in rat blood and all tested tissues with much higher enzyme activity in the blood,liver,kidney and heart than that in the small intestine,spleen,lung,pancreas,brain and *** addition,the four herbs showed tissue-specific effects on rat CES1 *** on the CES1 biodistribution and its changes after treatment in rats,the possibility that Danshen,gegen and Danggui might alter CES1 activities in human blood and kidney should be *** summary,a selective and sensitive bioluminescence assay was developed to rapidly evaluate CES1 activity and the effects of orally administered TCMs in rats.

关键词： Traditional Chinese medicines Carboxylesterase 1(CES1) NLMe Bioluminescence assay Biomatrice in rats

Vero细胞培养及其在病毒疫苗生产中的应用

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中兽医医药杂志 2021年第4期40卷 34-37页

作者：葛玉凤崔治亮武发菊龙玉红陶世宇刘学荣安芳兰中农威特生物科技股份有限公司甘肃兰州730046

Vero细胞因易于培养、稳定性强、增殖能力强等特性,广泛应用于狂犬病、流感及猪流行性腹泻病毒疫苗生产,是WTO批准的可用于人和动物疫苗生产的首选细胞系。就Vero细胞培养及其在病毒疫苗生产上的应用的研究进展予以综述,旨在为以Vero细... 详细信息

Vero细胞因易于培养、稳定性强、增殖能力强等特性,广泛应用于狂犬病、流感及猪流行性腹泻病毒疫苗生产,是WTO批准的可用于人和动物疫苗生产的首选细胞系。就Vero细胞培养及其在病毒疫苗生产上的应用的研究进展予以综述,旨在为以Vero细胞为基质的病毒性疫苗的研发提供参考。

关键词： Vero细胞疫苗生产研究进展应用

The Effect of the Cyp19a1 gene Methylation Modification on Temperature-dependent Sex Determination of Reeves' Turtle(Mauremys reevesii)

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Asian Herpetological Research 2017年第3期18卷 213-220页

作者： Wenxiu RU Liushuai HUA yufeng WEI Weiye LI Dainan CAO Yan ge Hong CHEN Xianyong LAN Shiping GONG Shaanxi Key Laboratory of Molecular Biology for Agriculture College of Animal Science and TechnologyNorthwest A&F University Guangdong Key Laboratory of Animal Conservation and Resource Utilization Guangdong Public Laboratory of Wild Animal Conservation and UtilizationGuangdong Institute of Applied Biological Resources Henan Key Laboratory of Farm Animal Breeding and Nutritional Regulation Institute of Animal Husbandry and Veterinary ScienceHenan Academy of Agricultural Sciences

Temperature-dependent sex determination（TSD） is a type of environmental sex determination in which the sex of the embryos depends on the ambient temperature; however,the molecular mechanisms governing this process remain ***,encoded by the cyp19a1 gene,which converts androgens into estrogens in animals,was considered to be the key gene for *** this study,the 5＇-flanking region of the cyp19a1 gene in Reeves＇ turtle（Mauremys reevesii） was cloned,and the promoter region was identified using the luciferase reporter *** the eggs of Reeves＇ turtle were incubated at different temperatures（26°C： male-biased temperature; 29°C： non-sex-biased temperature and 32°C： female-biased temperature）.During the thermosensitive period,the adrenal kidney gonad complexes（AKG） were *** methylation analysis of the AKG samples showed that the promoter region of the cyp19a1 gene was significantly de-methylated in the female-biased temperature regime（P＆lt;0.01）.Quantitative analysis of the cyp19a1 gene and estrogen by q PCR and Elisa assay showed that the expression level of the cyp19a1 gene and estrogen content were both upregulated significantly at the female-biased temperature（P＆lt;0.01）.These results indicated that the de-methylation response of the cyp19a1 gene to incubation temperature,especially at the female-biased temperature,could lead to temperature-specific expression of aromatase and increased estrogen levels,which may further determine gonadal development in Reeves＇ *** findings provide insights into the genetic mechanisms underlying TSD.

关键词： Reeves' turtle Mauremys reevesii temperature-dependent sex determination (TSD) cyp19a1 gene,methylation

加速康复外科理念下骨盆骨折的围手术期康复措施:一项前瞻性随机对照研究

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中华创伤骨科杂志 2021年第10期23卷 850-855页

作者：王金辉葛宇峰郭险峰陶莉刘晓华李蔷王宇章杨明辉朱仕文王振中金霖侯志勇贾健王连成余斌吴新宝北京积水潭医院创伤骨科 100035 北京积水潭医院康复医学科 100035 河北医科大学第三医院创伤骨科石家庄050051 天津医院创伤骨科 300211 南方医科大学南方医院骨科-创伤骨科广州510515

目的评价基于加速康复外科(ERAS)理念下的骨盆骨折围手术期康复措施的有效性和安全性。方法采用前瞻性随机对照研究的方法,纳入2019年6月至2020年12月北京积水潭医院创伤骨科急诊收治入院行手术治疗的114例骨盆骨折患者,按照随机数字表... 详细信息

目的评价基于加速康复外科(ERAS)理念下的骨盆骨折围手术期康复措施的有效性和安全性。方法采用前瞻性随机对照研究的方法,纳入2019年6月至2020年12月北京积水潭医院创伤骨科急诊收治入院行手术治疗的114例骨盆骨折患者,按照随机数字表法分为试验组57例,男42例,女15例;年龄18~77岁;患者采用初步建立的基于ERAS理念的围术期康复途径进行治疗,阶段性调整康复计划;对照组57例,男40例,女17例;年龄17~70岁;患者围手术期采用常规骨盆骨折康复措施,即术后院内会诊-康复医师指导模式。比较两组患者术后2、6、12、24周的骨盆Majeed评分、Barthel指数,以及术后12、24周的早期疼痛程度、健康状况调查简表(SF-36)。结果共105例患者完成随访,时间151~254 d(平均177 d),其中试验组55例,对照组50例。两组患者术前一般资料比较差异均无统计学意义(P>0.05),具有可比性。术后2、6、12、24周试验组患者的Majeed评分[(44±13)、(67±16)、(86±14)、(98±7)分]和Barthel指数(57±13、79±16、95±8、100±2)均显著高于对照组[(35±16)、(51±16)、(73±14)、(91±12)分和(45±19、67±18、86±12、98±4)],术后12、24周试验组患者的SF-36评分[(129±15)、(141±6)分]均显著高于对照组[(114±15)、(131±12)分],差异有统计学意义(P0.05)。结论与常规骨盆骨折治疗相比,基于ERAS理念的围手术期康复途径可以改善骨盆骨折患者的早期功能,有助于患者尽早恢复日常生活自理能力。

关键词：骨盆骨折加速康复外科骨科康复随机对照试验