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Chinese Journal of Integrative Medicine 2017年第3期23卷 221-225页

作者： CHEN Peng SHEN Qin-xuan SHEN Lin-yan WU Zhi-bing PI Li-hong ge wen-hua QI Wei Department of Neurology the First Clinical Medical College of Guangzhou University of Chinese Medicine Guangzhou(510006) China Department of Rehabilitation Medicine the First Affiliated Hospital of Guangzhou Medical University Guangzhou (510006) China

Cerebellar infarction accounts for almost 3%of all ischemic strokes,with 27,400 new cases each year in the USA alone.（1）Since the computerized tomography（CT）scan is often negative in the early hours after onset,it... 详细信息

关键词： computerized cerebellar alone consciousness decoction stasis vomiting intravenous infusion coagulation

Regulation of Tau Protein on the Antidepressant Effects of Ketamine in the Chronic Unpredictable Mild Stress Model

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神经药理学报 2019年第Z1期 90-91页

作者： wen ge-hua YAO Hui LI Yan-ning DING Run-tao REN Xing-hua TAN Ya-qing REN Wei-shu YU Hao ZHAN Xiao-ni WANG Xiao-long XU En-yu YAO Jun ZHANG Guo-hua LU Yan WU Xu School of Forensic Medicine China Medical University Department of Forensic Medicine School of Basic MedicineGannan Medical University Key Laboratory of Health Ministry in Congenital Malformation the Affiliated Sheng jing Hospital of China Medical University

Tau protein is known to play an important role in maintaining microtubule assembly and stabilization,and maintaining the normal morphology of neurons,but several studies have found that chronic stress leads to Tau hyperphosphorylation. A large number of clinical trials have found that ketamine,which is an N-methy-D-aspartate receptor antagonist,produces a rapid,long-lasting,and potent antidepressant effect in patients suffering from major depression. This rapid antidepressant effect of ketamine,which involves many mechanisms,has attracted wide attention. However,the relationship between ketamine’s antidepressant effects and Tau protein has rarely been examined. We used C57 BL/6 and Tau KO mice exposed to 42 days of chronic unpredictable mild stress(the CUMS model) to investigate the effect of ketamine on behavioral changes and synaptic functioning of the hippocampus. The results showed that a single treatment of ketamine rapidly relieved the CUMS-induced anhedonia,depression-like,and anxious behaviors of the C57 BL/6 mice. The abnormal behaviors were accompanied by increased levels of specific alterations of hyperphosphorylated Tau protein in cytoplasm and synapse in the hippocampus of the C57 BL/6 mice,but ketamine reduced the aggregation of hyperphosphorylated Tau protein only in the synapse. We also found that CUMS exposure reduced the levels of GluA1 and PSD95 in the hippocampus of the C57 BL/6 mice and that these deficits were reversed by ketamine. However,the Tau KO mice did not develop any stress-induced depressive behaviors or deficits of hippocampal function. The antidepressant effect of ketamine may decrease the levels of hyperphosphorylated Tau protein in synapse of C57 BL/6 mice.

关键词： depression hippocampus Tau protein phosphorylation synapse ketamine

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企业员工组织承诺表征与测度研究

青岛农业大学学报（社会科学版） 2011年第4期23卷 70-73页

作者：葛青华林盛葛文华天津大学管理与经济学部天津300072 山东安和乐利有限公司山东济南250101 青岛农业大学人事处山东青岛266109

以企业员工组织承诺表征为切入点,从情感承诺、持续承诺和规范承诺三个方面阐述了企业员工组织承诺的表征变量体系及内涵,并运用层次分析法,研究了某企业员工组织承诺及其重要程度二维矩阵。结果表明,通过对组织承诺的表征变量及其重要... 详细信息

以企业员工组织承诺表征为切入点,从情感承诺、持续承诺和规范承诺三个方面阐述了企业员工组织承诺的表征变量体系及内涵,并运用层次分析法,研究了某企业员工组织承诺及其重要程度二维矩阵。结果表明,通过对组织承诺的表征变量及其重要程度的科学测度,可以将企业员工对组织目标与价值的认同状态,以及员工促进组织发展的内在动因客观地表现出来,此项研究为企业人力资源管理部门制定决策提供了科学依据。

关键词：企业组织承诺员工忠诚工作绩效

Return to work in young and middle-aged colorectal cancer survivors:Factors influencing self-efficacy,fear,resilience,and financial toxicity

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World Journal of Gastroenterology 2025年第1期31卷 79-92页

作者： Dan Hu Yue Li hua Zhang Lian-Lian Wang wen-wen Liu Xin Yang Ming-Zhao Xiao Hao-Ling Zhang Juan Li Department of Nursing The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Nursing National Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChildren’s Hospital of Chongqing Medical UniversityChongqing 400014China Department of Gastrointestinal Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Biomedical Science Advanced Medical and Dental InstituteUniversity Sains MalaysiaPenang 13200Malaysia Academic Affairs Office The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

BACKGROUND Return to work(RTW)serves as an indication for young and middle-aged colorectal cancer(CRC)survivors to resume their normal social ***,these survivors encounter significant challenges during their RTW ***,scientific research is necessary to explore the barriers and facilitating factors of returning to work for young and middle-aged CRC *** To examine the current RTW status among young and middle-aged CRC survivors and to analyze the impact of RTW self-efficacy(RTW-SE),fear of progression(FoP),eHealth literacy(eHL),family resilience(FR),and financial toxicity(FT)on their RTW *** A cross-sectional investigation was adopted in this *** September 2022 to February 2023,a total of 209 participants were recruited through a convenience sampling method from the gastrointestinal surgery department of a class A tertiary hospital in *** investigation utilized a general information questionnaire alongside scales assessing RTW-SE,FoP,eHL,FR,and *** analyze the factors that influence RTW outcomes among young and middle-aged CRC survivors,Cox regression modeling and Kaplan-Meier survival analysis were *** A total of 43.54%of the participants successfully returned to work,with an average RTW time of 100 *** regression univariate analysis revealed that RTW-SE,FoP,eHL,FR,and FT were significantly different between the non-RTW and RTW groups(Pged CRC *** professionals should focus on modifiable factors,such as RTW-SE and FR,to design targeted RTW support programs,thereby facilitating their timely reintegration into mainstream society.

关键词： Return to work Colorectal neoplasms Return-to-work self-efficacy Fear of progression Family resilience Financial toxicity

Effects of ketamine on mouse hippocampal inflammatory cytokines IL-6,IL-1β and TNF-α levels in acute and chronic administration models

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中国药理学与毒理学杂志 2017年第5期31卷 476-476页

作者： LI Yan-ning SHEN Rui-peng wen ge-hua DING Run-tao DU Ao ZHOU Ji-chuan DONG Zhi-bin REN Xing-hua YAO Hui ZHAO Rui ZHANG Guo-hua LU Yan WU Xu Department of Forensic Pathology School of Forensic MedicineChina Medical University Wujiang District Branch of Suzhou Public Security Bureau Key Laboratory of Health Ministry in Congenital Malformation the Affiliated Shengjing Hospital of China Medical University

OBJECTIVE Ketamine is an injectable anesthetic and recreational drug of abuse commonly used worldwide. Many experimental studies have shown that ketamine can impair cognitive function and induce psychotic states. Neuroinflammation has been suggested to play an important role in neurodegeneration. Meanwhile,ketamine has been showed to modulate the levels of inflammatory ***,we sought to investigate whether the effects of ketamine on the central nervous system is associated with the inflammatory cytokines. METHODS We established acute(single or multiple intraperitoneal injection) and chronic(six months daily intraperitoneal injection) ketamine administration models in C57BL/6 mice,evaluated the spatial recognition memory and emotional response by applying the Y maze test and open field test. We analyzed the changes of inflammatory cytokines interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) levels in mouse hippocampus,employing Western blot,quantitative reverse transcriptase-polymerase chain reaction(qR T-PCR) and immunohistochemistry. RESULTS Ketamine induced spatial recognition memory deficit,reduced anxiety-like behaviors in mice after chronic administration,and it was dose-dependent. Moreover,we found that ketamine could increase the levels of mouse hippocampal inflammatory cytokines IL-6 and IL-1β after single,multiple and long-term administration in a dose-dependent manner. However,the level of TNF-α expressed differently in mouse hippocampus under different conditions. Single administration of ketamine increased the level of TNF-α,whereas multiple and long-term administration decreased it significantly. We considered that TNF-α might exist bi-directional regulatory pathway,which was associated with the dose and duration of ketamine administration. CONCLUSION Our results suggest that the alterations of inflammatory cytokines IL-6,IL-1β and TNF-α levels may be involved in the neurotoxicity of ketamine.

关键词： ketamine behavior IL-6 IL-1β TNF-α

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2型糖尿病与系统思维

山东中医杂志 2000年第11期19卷 643-644页

作者：岳桂华盖欣张爱珍宋文杰葛文华利津县中心医院山东利津257400 山东中医药大学山东济南250014

从系统论整体性、自组织性原理阐述了正常血糖的波动是机体在强序状态下自组织的结果。认为 2型糖尿病是在基因异常基础上的多级子系统异常的巨系统疾病 ,并认为整体治疗 ,增强机体的自主调节能力 ,是 2型糖尿病的有效治疗手段。

关键词： 2型糖尿病发病治疗整体性原理自组织性原理

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烟酸对五龙鹅抗氧化性能和免疫性能的影响

中国畜牧杂志 2016年第1期52卷 32-37页

作者：段晨磊王宝维葛文华张名爱李文立青岛农业大学动物科技学院山东青岛266109 青岛农业大学优质水禽研究所山东青岛266109

本试验旨在研究日粮中烟酸水平对五龙鹅抗氧化和免疫性能的影响。选用1日龄五龙鹅360只，随机分为6组，每组6重复，每重复10只。I组为对照组，饲喂基础日粮（烟酸含量1～4周龄25．54mg／kg，5-16周龄23．48mg／kg）。Ⅱ～Ⅵ组在基础日... 详细信息

本试验旨在研究日粮中烟酸水平对五龙鹅抗氧化和免疫性能的影响。选用1日龄五龙鹅360只，随机分为6组，每组6重复，每重复10只。I组为对照组，饲喂基础日粮（烟酸含量1～4周龄25．54mg／kg，5-16周龄23．48mg／kg）。Ⅱ～Ⅵ组在基础日粮中分别添加20、40、60、80、100mg／kg的烟酸，试验期为16周。结果表明：4周龄．添加80mg／kg烟酸板显著提高血清和肝脏谷胱甘肽过氧化物酶（GSH—Px）活性（P〈0．01），极显著降低肝脏丙二醛（MDA）、过氧化氢（H2O2）含量（P〈0．01），极显著提高总抗氧化能力（T—AOC）（P〈0．01）；16周龄，添加80mg／kg极显著提高血清总超氧化物歧化酶（T—SOD）和肝脏T—SOD、GSH—Px、过氧化氢酶（CAT）活性（P〈0．01），极显著降低血清和肝脏MDA含量（P〈0．01），极显著提高T—AOC（P〈0．01）。添加80mg／kg烟酸极显著提高4周龄胸腺跟法氏囊指数（P〈0．01），显著提高4周龄脾脏指数和16周龄胸腺指数（P〈0．05）。添加80mg／kg烟酸显著提高禽流感抗体滴度和外周血淋巴细胞转化率（P〈0．05）。本试验条件下，1—16周龄五龙鹅日粮烟酸添加量均以80mg／kg为宜。

关键词：烟酸鹅抗氧化性能免疫性能

鼠艾滋病病毒荧光定量PCR标准品RNA的构建

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热带医学杂志 2009年第4期9卷 392-394,353页

作者：葛文华何金洋广州中医药大学温病教研室广州510405 广州中医药大学热带医学研究所广州510405

目的合成构建鼠艾滋病病毒(FLV)荧光定量检测标准品,明确核糖核酸(RNA)标准品体外合成的基本原则。方法通过序列对比,找到FLV最为保守的区段作为RNA标准品的合成目标,进行逆转录聚合酶链反应(RT-PCR)扩增,克隆于T载体,测序,设计带有T7... 详细信息

目的合成构建鼠艾滋病病毒(FLV)荧光定量检测标准品,明确核糖核酸(RNA)标准品体外合成的基本原则。方法通过序列对比,找到FLV最为保守的区段作为RNA标准品的合成目标,进行逆转录聚合酶链反应(RT-PCR)扩增,克隆于T载体,测序,设计带有T7启动子的引物,PCR扩增,将所得的PCR产物凝胶回收纯化,用T7RNA聚合酶进行体外合成RNA,最后用PCR和电泳检测验证。结果成功合成了FLV荧光定量PCR标准品。结论FLV荧光定量标准品的成功合成必须遵守以下4条基本原则:①选择保守序列;②设计引物时将潜在的终止密码或起始密码排除在PCR目标产物之外;③将启动子(如T7启动子)挂在适当的引物上,保证所合成的RNA模板与所要定量检测的RNA模板一致,而不是互补;④合成RNA后必须检测,以保证不含其他RNA或者未消化完的DNA。

关键词：鼠艾滋病病毒 RNA病毒逆转录聚合酶链反应

SIV感染CEM×174细胞后CD28家族免疫调控分子mRNA的动态变化

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山西医科大学学报 2009年第5期40卷 429-432页

作者：葛文华何金洋广州中医药大学温病教研室广州510405 广州中医药大学热带医学研究所

目的观察猴免疫缺陷病毒(SIV)感染CEM×174细胞后CD28家族免疫调控分子CD28、BTLA、ICOS、PD-1 mRNA表达水平的动态变化。方法以SIVmac251感染接种CEM×174细胞,收集感染后12,24,48,72,96,120,144,168,216h共8个时间点及正常未感染细胞... 详细信息

目的观察猴免疫缺陷病毒(SIV)感染CEM×174细胞后CD28家族免疫调控分子CD28、BTLA、ICOS、PD-1 mRNA表达水平的动态变化。方法以SIVmac251感染接种CEM×174细胞,收集感染后12,24,48,72,96,120,144,168,216h共8个时间点及正常未感染细胞,提取细胞RNA,以染料法逆转录荧光定量PCR法检测SIV、CD28、ICOS、PD-1、BTLA mRNA的表达。结果SIV mRNA在48h时表达即显著升高(P<0.05),随着SIV mRNA量的逐渐上升,ICOS在48h时开始显著升高(P<0.05),而CD28、PD-1 mRNA在120h时才出现表达显著增加(P<0.05);BTLA mRNA则呈现下降趋势,并在168h开始出现显著下调(P<0.05)。结论除BTLA以外,所检测SIV感染后细胞中促进免疫激活和抑制免疫的免疫调节因子(ICOS、PD-1、CD28)表达均显著升高,提示AIDS中存在免疫紊乱。

关键词：艾滋病猴免疫缺陷病毒 CEM × 174细胞免疫调控分子

鼠艾滋病模型血浆病毒载量荧光定量PCR检测方法的建立

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中国热带医学 2009年第4期9卷 593-595,612页

作者：葛文华何金洋广州中医药大学温病教研室广东广州510405 广州中医药大学热带医学研究所广东广州510405

目的建立鼠艾滋病模型血浆病毒载量荧光定量聚合酶链(PCR)检测方法。方法将体外合成的鼠艾滋病病毒(FLV)核糖核酸(RNA)标准品稀释为10个浓度梯度,进行逆转录,PCR荧光定量检测,经批内与批间差异分析,再以PCR荧光定量检测不同倍数FLV脾悬... 详细信息

目的建立鼠艾滋病模型血浆病毒载量荧光定量聚合酶链(PCR)检测方法。方法将体外合成的鼠艾滋病病毒(FLV)核糖核酸(RNA)标准品稀释为10个浓度梯度,进行逆转录,PCR荧光定量检测,经批内与批间差异分析,再以PCR荧光定量检测不同倍数FLV脾悬液FLV拷贝数及相应被注射小鼠模型的血浆病毒载量,计算脾指数。结果研究设计的荧光定量PCR方法能检测到20～2×108copiesFLVRNA逆转录反应体系,在5个注射的FLV拷贝数范围内,血浆病毒载量与脾指数均不与初始注射的FLV拷贝数成正比,二者亦不呈现平行关系。结论研究采用的FLV荧光定量PCR方法敏感性好,特异性强,重现性好,显著提高FLV病毒的检测水平。

关键词：鼠艾滋病病毒荧光定量聚合酶联反应病毒载量