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Original article Inhibition of collagen-induced arthritis by DNA vaccines encoding TCR Vβ5.2 and TCR Vβ8.2

Chinese Medical Journal 2009年第9期122卷 1039-1048页

作者： ge ping-ling MA Li-ping WANG Wei LI Yun ZHAO Wen-ming Department of Transfusion Aerospace Center Hospital Beijing100049 China Department of Immunology Capital Medical University Beijing100069 China

Background Arthritogenic T lymphocytes with common T cell receptor （TCR） Vβ clonotypes, infiltrating in the articulars of rheumatoid arthritis （RA） patients, play a central role in the pathogenesis of RA. TCR Vβ5.2 and TCR Vβ8.2 are the main pathogenic T cell clonotypes in the course of collagen-induced arthritis （CIA） progression in Lewis rats. To investigate a TCR-based immunotherapy for RA, we constructed recombinant DNA vaccines encoding TCR Vβ5.2 and TCR Vβ8.2, and evaluated the inhibitive effects of the two vaccines on CIA rats.Methods genes encoding TCR Vβ5.2 and TCR Vβ8.2 were amplified by RT-PCR from spleen lymphocytes of Lewis rats and cloned into the eukaryotic expression vector pTargeT. The expression of vaccines was confirmed by RT-PCR and immunohistochemistry. The inhibitive effects of the vaccines on articulars of CIA rats were assessed with arthritis index evaluation and histology. Interferon γ （IFN-γ） and interleukin （IL）-4 production by spleen lymphocytes were tested with enzyme-linked immunospot assay （ELISPOT） technique, the changes in peripheral CD4^＋ and CD8^＋ lymphocyte populations were tested by flow cytometry, and the level of anti-CII antibody in serum was assayed by enzyme-linked immunosorbent assay （ELISA）.Results Recombinant DNA vaccines pTargeT-TCR Vβ5.2 and pTargeT-pTCR Vβ8.2 were successfully constructed. Both vaccines inhibited CIA, which alleviated the arthritis index score （P 〈0.05）, decreased the level of IFN-γ （P 〈0.05）, and reduced the ratio of CD4^＋/CD8^＋ lymphocytes （P 〈0.05） and the anti-CII antibody in serum （P 〈0.05）. In addition, the histological change in DNA-vaccinated rats was less serious than CIA rats. Compared to pTCR Vβ 8.2 and pTCR Vβ 5.2 groups, the group that was injected with a combination of the two vaccines showed stronger inhibitive effects on CIA than either individual vaccine.Conclusion The recombinant plasmids pTargeT-TCR Vβ5.2 and pTargeT-TCR Vβ8.2 have obvious inhibatory effects on CIA rats and bet

关键词： arthritis, collagen-induced T cell receptor vaccines, DNA

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Experimental investigation of dynamic characteristics of leaching tubing for solution mining of salt cavern carbon and energy storage

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Petroleum Science 2024年第4期21卷 2703-2722页

作者： Yin-ping Li Xin-Bo ge Xi-Lin Shi Hong-ling Ma State Key Laboratory of geomechanics and geotechnical Engineering Institute of Rock and Soil Mechanics Chinese Academy of Sciences University of Chinese Academy of Sciences College of Energy and Mining Engineering Shandong University of Science and Technology State Key Laboratory of Mining Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and Technology Shandong University of Science and Technology

Salt caverns are extensively utilized for storing various substances such as fossil energy, hydrogen,compressed air, nuclear waste, and industrial solid waste. In China, when the salt cavern is leached through single-well water solution mining with oil as a cushion, engineering challenges arise with the leaching tubing, leading to issues like damage and instability. These problems significantly hinder the progress of cavern construction and the control of cavern shape. The primary cause of this is the flowinduced vibration instability of leaching tubing within a confined space, which results in severe bending or damage to the tubing. This study presents a model experimental investigation on the dynamic characteristics of leaching tubing using a self-developed liquid-solid coupling physical model experiment apparatus. The experiment utilizes a silicone-rubber pipe(SRP) and a polycarbonate pipe(PCP) to examine the effects of various factors on the dynamic stability of cantilevered pipes conveying fluid. These factors include external space constraint, flexural rigidity, medium outside the pipe, overhanging length, and end conditions. The experiments reveal four dynamic response phenomena: water hammer, static buckling, chaotic motion, and flutter instability. The study further demonstrates that the length of the external space constraint has a direct impact on the flutter critical flow velocity of the cantilevered pipe conveying fluid. Additionally, the flutter critical flow velocity is influenced by the end conditions and different external media.

关键词： Salt cavern storage Solution mining Leaching tubing Experimental investigation Flutter instability

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Optimized the vanadium electrolyte with sulfate-phosphoric mixed acids to enhance the stable operation at high-temperature

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Frontiers of Chemical Science and Engineering 2024年第2期18卷 13-26页

作者： ling ge Tao Liu Yimin Zhang Hong Liu School of Resource and Environmental Engineering Wuhan University of Science and TechnologyWuhan 430081China State Environmental Protection Key Laboratory of Mineral Metallurgical Resources Utilization and Pollution Control Wuhan University of Science and TechnologyWuhan 430081China Hubei Collaborative Innovation Center for High Efficient Utilization of Vanadium Resources Wuhan University of Science and TechnologyWuhan 430081China Hubei Provincial Engineering Technology Research Center of High Efficient Cleaning Utilization for Shale Vanadium Resource Wuhan University of Science and TechnologyWuhan 430081China School of Resource and Environmental Engineering Wuhan University of TechnologyWuhan 430070China

Herein, the influence of the concentration design and comprehensive performance of the sulfate-phosphoric mixed acid system electrolyte is investigated to realize an electrolyte that maintains high energy density and stable operation at high temperatures. Static stability tests have shown that VOPO4 precipitation occurs only with vanadium(V) electrolyte. The concentration of vanadium ion of 2.0–2.2 mol·L^(–1), phosphoric acid of 0.10–0.15 mol·L^(–1), and sulfuric acid of 2.5–3.0 mol·L^(–1) are suitable for a vanadium redox flow battery in the temperature range from –20 to 50 ℃. The equations for predicting the viscosity and conductivity of electrolytes are obtained by the response surface method. The optimized electrolyte overcomes precipitation generation. It has 2.8 times higher energy density than the non-phosphate electrolyte, and a coulomb efficiency of 94.0% at 50 ℃. The sulfate-phosphoric mixed acid system electrolyte promotes the electrode reaction process, increases the current density, and reduces the resistance. This work systematically optimizes the concentrations of composition of positive and negative vanadium electrolytes with mixed sulfate-phosphoric acid. It provides a basis for the different valence states and comprehensive properties of sulfate-phosphoric mixed acid system vanadium electrolytes under extreme environments, guiding engineering applications.

关键词： all vanadium redox flow battery mixed-acid vanadium electrolyte concentration optimization response surface methodology high-temperature stability

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Revealing effect of Al content on oxidation of novel Co-Cr-Nb-W carbide-strengthened superalloy

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Rare Metals 2024年第9期43卷 4521-4534页

作者： Chen ling Shang-ping Li Jie Hou He-Li Luo Central Iron & Steel Research Institute Beijing GAONA Materials&Technology Co. Ltd.

The high-temperature oxidation behavior of novel Co-Cr-Nb-W carbide-strengthened wear-resistance alloys with different Al contents（1wt%, 2wt% and 3wt%）at 950, 1000 and 1050 °C was thoroughly investigated by scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and field emission electron probe analyzer. The porous property of Nb C in-situ oxidation products（Nb2O5, Co Nb2O6and Co4Nb2O9） induces a multi-layered oxide scale with micropores and ***-Cr-Nb-W alloy with 1 wt% Al undergoes catastrophic oxidation and spalling above 1000 °C. The outward transportation of Cr and Co is effectively restrained by a continuous Al2O3scale formed around Nb C in-situ oxidation region when Al content reaches 3 wt%. The β-Co Al in Co-Cr-Nb-W alloy with 3 wt% Al has an oxidation priority over eutectic carbides and the alloy matrix which are both enriched with Cr, thereby preventing the formation of Cr-depletion area and improving the self-healing ability of the oxide film. A slight change in Al content has a remarkable influence on the cooperative effect of Al and Cr and multiplies the antioxidant capacity of Co-Cr-Nb-W alloy above 1000 °C.

关键词： High-temperature oxidation behavior Microstructure Oxidation kinetics Superalloy Wear-resistant alloy

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Cross-species transmission and host range genes in poxviruses

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Virologica Sinica 2024年第2期39卷 177-193页

作者： Chen-Hui Yang A-ling Song Ye Qiu Xing-Yi ge College of Biology Hunan Provincial Key Laboratory of Medical VirologyHunan UniversityChangsha410012China

The persistent epidemic of human mpox, caused by mpox virus (MPXV), raises concerns about the future spread ofMPXV and other poxviruses. MPXV is a typical zoonotic virus which can infect human and cause smallpox-likesymptoms. MPXV belongs to the Poxviridae family, which has a relatively broad host range from arthropods tovertebrates. Cross-species transmission of poxviruses among different hosts has been frequently reported andresulted in numerous epidemics. Poxviruses have a complex linear double-strand DNA genome that encodeshundreds of proteins. genes related to the host range of poxvirus are called host range genes (HRGs). This reviewbriefly introduces the taxonomy, phylogeny and hosts of poxviruses, and then comprehensively summarizes thecurrent knowledge about the cross-species transmission of poxviruses. In particular, the HRGs of poxvirus aredescribed and their impacts on viral host range are discussed in depth. We hope that this review will provide acomprehensive perspective about the current progress of researches on cross-species transmission and HRGvariation of poxviruses, serving as a valuable reference for academic studies and disease control in the future.

关键词： Poxvirus Host range gene(HRG) Host spectrum Cross-species transmission

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Glaucoma drainage device implantation and cyclophotocoagulation in the management of refractory glaucoma after Descemet-stripping automated endothelial keratoplasty

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International Journal of Ophthalmology(English edition) 2024年第2期17卷 257-264页

作者： Min-Shu Wang Xue-Chuan Dong Mi-Yun Zheng Xiang Fan ge-ge Xiao Jing Hong ling-ling Wu Department of Ophthalmology Peking University Third HospitalBeijing 100191China Key Laboratory of Restoration of Damaged Ocular Nerve Beijing 100191China NIMO Ophthalmology Research Institute Beijing 100176China Department of Ophthalmology the First Hospital of PutianTeaching HospitalFujian Medical UniversityPutian 351100Fujian ProvinceChina

AIM:To compare the surgical outcomes of glaucoma drainage device implantation(GDI)and trans-scleral neodymium:YAG cyclophotocoagulation(CPC)in the management of refractory glaucoma after Descemetstripping automated endothelial keratoplasty(DSAEK).METHODS:This retrospective study on observational case series enrolled 29 patients who underwent DSAEK and posterior anti-glaucoma surgery(15 with GDI and 14 with CPC).The main outcome measures were intraocular pressure(IOP),glaucoma surgery success rate(defined as IOP of 6–21 mm Hg without additional anti-glaucoma operation),number of glaucoma medications,endothelial graft status,and best-corrected visual acuity(BCVA).RESULTS:The mean follow-up time was 34.1 and 21.0mo for DSAEK or glaucoma surgeries,both for the GDI and CPC *** groups showed significant IOP reduction after glaucoma *** GDI group presented a significantly higher success rate in IOP control than the CPC group(60%vs 21.4%,P=0.03).Both procedures significantly decreased the number of glaucoma medications(P=0.03).Forty percent and 57%of cases in the GDI and the CPC group,respectively,experienced endothelial graft failure during follow-up(P=0.36).Significantly worse BCVA after surgery was observed in the CPC group but not in the GDI ***:Both GDI and CPC significantly decrease IOP in eyes with glaucoma after *** is preferable to CPC in refractory glaucoma cases after DSAEK,as it manifests a significantly higher success rate for IOP control,similar endothelial graft failure rate,and relatively preserves BCVA than CPC.

关键词： glaucoma drainage device implantation cyclophotocoagulation refractory glaucoma Descemetstripping automated endothelial keratoplasty

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A hybrid data-mining framework for train rescheduling strategy pattern discovery

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Transportation Safety and Environment 2024年第1期6卷 56-67页

作者： Ruirui Chen Xuekai ge ping Huang Chao Wen School of Transportation&Logistics Southwest Jiaotong UniversityChengdu 611756China Department of IT Operational Technology CN RailWaterloo N2V2R1Canada

This study presents a hybrid data-mining framework based on feature selection algorithms and clustering methods to perform the pattern discovery of high-speed railway train rescheduling strategies(RSs).The proposed model is composed of two *** the first state,decision tree,random forest,gradient boosting decision tree(GBDT)and extreme gradient boosting(XGBoost)models are used to investigate the importance of *** features that have a high influence on RSs are first *** the second state,a K-means clustering method is used to uncover the interdependences between RSs and the influencing features,based on the results in the first *** proposed method can determine the quantitative relationships between RSs and influencing *** results clearly show the influences of the factors on RSs,the possibilities of different train operation RSs under different situations,as well as some key time periods and key trains that the controllers should pay more attention *** research in this paper can help train traffic controllers better understand the train operation patterns and provides direction for optimizing rail traffic RSs.

关键词： high-speed railway rescheduling strategy pattern discovery feature identification clustering algorithm

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Investigating effect of coke porosity on blast furnace performance based on multi-physical fields

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Journal of Iron and Steel Research International 2024年第2期31卷 357-367页

作者： Ji Chen Zhao Lei Zhe Yao Li Wang Qiang ling ping Cui Anhui Province Key Laboratory of Coal Clean Conversion and High Valued Utilization School of Chemistry and Chemical EngineeringAnhui University of TechnologyMa’anshan243002AnhuiChina Shanxi Academy of Printing Technology Research Institute Co. Ltd.Xi’an710077ShaanxiChina

Reducing coke use is an effective measure to reduce carbon emission and energy consumption in the blast furnace(BF)***,BF is a high-temperature moving bed reactor,where complex physical transformations coupled with complicated reactions *** makes it challenging to investigate the factors determining BF performance with the conventional method.A multi-physical field coupling mathematical model of BF was thus developed to describe its mass and heat transfer as well as its intrinsic ***,the proposed model was validated with the production *** coupling conditions,influences of dominating reactions on BF performance(temperature distribution,gas distribution,iron formation reaction,and direct reduction degree)were *** results indicated that coke combustion,indirect reduction,and direct reduction of iron ore mainly took place nearby the shaft tuyere,cohesive zone,and dripping zone,***,the rate of coke solution loss reaction was increased with the rising coke porosity in the cohesive *** the effect of coke porosity on the efficiency and stability of BF,the coke porosity of 0.42 was regarded as a reasonable value.

关键词： Blast furnace Mathematical model Multi-physical field Simulation Coke porosity

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Three types of promising functional building units for designing metal halide nonlinear optical crystals

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结构化学 2024年第6期43卷 8-11页

作者： Jiajing Wu Ru-ling Tang Sheng-ping Guo School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou225002PR China School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou225002PR China Yunnan Key Laboratory of Electromagnetic Materials and Devices National Center for International Research on Photoelectric and Energy MaterialsSchool of Material and EnergyYunnan UniversityKunming650000PR China

Nonlinear optical(NLO)crystal materials greatly extend the tunable range of laser by frequency conversion,which promotes the rapid development of laser technology and plays an important role in many civil and military *** excellent and famous NLO crystals from the ultraviolet(UV)to visible to near infrared(NIR)regions have been developed,such as borates(KBe2BO3F2,β-BaB2O4),phosphates(KH2PO4,KTiOPO4),chalcogenides(AgGaS2,AgGaSe2)and so on[1,2].

关键词： optical tunable designing

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Observation and Analysis of VLF Nocturnal Multimode Interference Phenomenon based on Waveguide Mode Theory

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Research in Astronomy and Astrophysics 2024年第1期24卷 78-86页

作者： Sai Yang You-Tian Niu Zhe Wang Xiu-Kun Zhao Bei Li Yu-ling Ding ge-ge Zhao An-Qi Zhang College of Electronic and Electrical Engineering Henan Normal UniversityXinxiang 453007China Academician Workstation of Electromagnetic Wave Engineering of Henan Province Xinxiang 453007China Key Laboratory of Optoelectronic Sensing Integrated Application of Henan Province Xinxiang 453007China Engineering Laboratory of Intelligent Additive Manufacturing of Henan Province Xinxiang 453007China

Very low frequency(VLF)signals are propagated between the *** interference will cause the phase to show oscillatory changes with distance while propagating at night,leading to abnormalities in the received VLF *** study uses the VLF signal received in Qingdao City,Shandong Province,from the Russian Alpha navigation system to explore the multimode interference problem of VLF signal *** characteristics of the effect of multimode interference phenomena on the phase are analyzed according to the variation of the phase of the VLF ***,the phase of VLF signals will also be affected by the X-ray and energetic particles that are released during the eruption of solar flares,therefore the two phenomena are studied in this *** is concluded that the X-ray will not affect the phase of VLF signals at night,but the energetic particles will affect the phase change,and the influence of energetic particles should be excluded in the study of multimode interference *** VLF signals for navigation positioning in degraded or unavailable GPS conditions is of great practical significance for VLF navigation systems as it can avoid the influence of multimode interference and improve positioning accuracy.

关键词： waves methods:data analysis Sun:flares Sun:X-rays gamma-rays

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