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Journal of Future Foods 2024年第2期4卷 149-158页

作者： Zhihui Yu yating gao Meng Wu Chaofan Zhao Xiubin Liu Xiaoyu Zhang Lixin Zhang Yisheng Chen College of Food Science and Engineering Shanxi Agricultural UniversityTaigu 030801China College of Animal Science and Technology Hunan Agricultural UniversityChangsha 410128China Houji Laboratory in Shanxi Province Shanxi Agricultural UniversityTaiyuan 030031China

This study aimed to investigate the effects of phosphorylated-ovalbumin(P-OVA)at different concentrations(0.5%and 1.0%)on the gel properties of pork myofibrillar protein(MP).The results showed that the textural properties such as gel hardness,cohesiveness,springiness and chewiness were improved with P-OVA addition at 0.5%.The water holding capacity(up to 75.89%)and gel strength(up to 168.56 g·mm)of MP gel were markedly increased after P-OVA *** absolute value of zeta potential reached 13.85 mV and maximum hydrophobicity(15.2μg)resulted from the addition of 0.5%*** storage modulus(G’)and loss modulus(G’’)of MP gel were significantly increased from 50°C,and the G’and G’’significantly increased after 1.0%P-OVA addition,evidencing that the cross-linking effect of MP protein gel was *** addition,the P-OVA addition improved the structure of MP gel protein by reducing theα-helix,while increasing theβ-sheet and the r-value(the ratio between two ultraviolet second-derivative peak-to-trough distances),which further promoted the uniform and compact gel network *** work demonstrated that P-OVA is a highly effective modifier that significantly improves the quality of MP *** a view of practice,0.5%P-OVA is the optimal addition amount.

关键词： Phosphorylated ovalbumin Myofibrillar proteins Gel properties Structures

Insight into effects of egg- and soybean-derived phosphatidylcholine on non-alcoholic steatosis in mice and HepG2 cells by lipidomics

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Food Science and Human Wellness 2024年

作者： Zhihui Yu Chunyou Liu yating gao Haoran Guo Huiling Duan Lixin Zhang Yisheng Chen College of Food Science and Engineering， Shanxi Agricultural University Institute of Food Nutrition and Safety， Shanxi Agricultural University Department of Food Science and Technology， Guangxi University of Science and Technology

Dietary phosphatidylcholine (PC) is an ideal source of bioactive lipids showing efficacy to alleviate non-alcoholic fatty liver disease (NAFLD). In this study， the lipidomic profiles of nonalcoholic steatosis in mice and HepG2 cells treated with PC isolated from eggs (EPC) and soybean (SPC) were evaluated. EPC and SPC could significantly ameliorate fatty liver disease by down-regulating triglyceride (TG) and diglyceride (DG) levels. Remarkably， EPC exhibited better performance in regulating the hepatic lipid profile leading to decreased PC and TG， ceramides， phosphatidylethanolamine and phosphatidylserine levels. A total of 117 and 20 lipid biomarkers in liver and HepG2 cells involved in glycerophospholipid metabolism were screened out， respectively. The regulatory mechanism of EPC may be attributed to a decrease in the expression of ACACA and SREBP-1c. EPC， via suppressing lipogenic gene expression， which was superior to that of SPC in alleviating nonalcoholic steatosis. Overall， EPC and SPC attenuated HFD-induced NAFLD by improving the hepatic lipid profile. The more significant effect observed with EPC may be attributed to its modulation of glycerophospholipid metabolism.

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Uncovering the action mechanism of Shenqi Tiaoshen formula(参芪调肾方) in the treatment of chronic obstructive pulmonary disease through network pharmacology,molecular docking,and experimental verification

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Journal of Traditional Chinese Medicine 2024年第4期44卷 770-783页

作者： YANG Qinjun YIN Dandan WANG Hui gao yating WANG Xinheng WU Di TONG Jiabing WANG Chuanbo LI Zegeng School of Chinese Medicine Anhui University of Chinese MedicineHefei 230038China Anhui Province Key Laboratory of the Application and Transformation of Traditional Chinese Medicine in the Prevention and Treatment of Major Pulmonary Diseases Hefei 230031China Department of Pharmacy the First Affiliated Hospital of Anhui Medical UniversityHefei 230022China Institute of Traditional Chinese Medicine Respiratory Disease Prevention and Control Anhui Academy of Traditional Chinese MedicineHefei 230031China Department of Respiratory the First Affiliated Hospital of Anhui University of Chinese MedicineHefei 230031China Department of Chinese Medicine the Second Affiliated Hospital of Anhui Medical UniversityHefei 230601China

OBJECTIVE:To reveal the potential underlying mechanism of the Shenqi Tiaoshen formula(参芪调肾方,SQTS)in the treatment of chronic obstructive pulmonary disease(COPD)by utilizing network pharmacology,molecular docking,and experimental ***:Multiple open-source databases and research related to Traditional Chinese Medicine or compounds were employed to screen active ingredients and corresponding potential targets of the *** protein-protein interaction network screened hub genes,the relevant molecular mechanism and gene regulation were initially identified through the Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)analysis,and molecular docking was used to confirm further the interaction of the main components bound to the core *** vivo experiments on the COPD combined Qi-deficiency syndrome rat model were performed to verify the intervention effects and predicted potential molecular mechanisms of the ***:This study selected 156 active compounds and 326 candidate targets for treating ***,Nobiletin,Kaempferol,Luteolin,Ginsenoside Rh2 and Formononetin were probably the main active compounds of SQTS in COPD treatment as they affected the most COPD-related targets,and interleukin-1(IL-6),signal transducing activator of transcription 3(STAT3),matrix metalloproteinase-9(MMP9),vascular endothelial growth factor A(VEGFA),protein kinase B(AKT1),hypoxiainducible factor-1α(HIF-1α),and forkhead box O3(FoxO3)were identified as the hub genes associated with its therapeutic *** analysis was mainly enriched in the signaling pathways closely related to inflammation,immunity and oxidative stress,such as HIF-1,tumor necrosis factor(TNF),phosphatidylinositol 3 kinase(PI3K)-AKT,FoxO,apoptosis,IL-17,and toll-like *** docking confirmed that the main active components shared a good affinity with the hub *** vivo experiments,the SQTS was found to improve the body weight,exhaustive swimming time,tailhangin

关键词： inflammation immunity treatment

β-岚香酮酸对肝细胞癌细胞增殖和凋亡的影响及其机制

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基因组学与应用生物学 2024年第5期43卷 907-924页

作者：邹晨乔凤杰方淼高亚婷马言璐高月求张鑫上海中医药大学附属曙光医院细胞免疫实验室上海市中医临床重点实验室上海201203 上海中医药大学附属曙光医院肝病科上海201203

本研究旨在探讨β-岚香酮酸(β-elemonic acid,β-EA)对肝细胞癌(hepatocellular carcinoma,HCC)细胞增殖和凋亡的影响及其作用机制。利用CCK8法检测β-EA对人HCC细胞Huh7和PLC/PRF/5活力的影响;利用Annexin V-FTTC/7AAD染色检测细胞凋亡变化;利用流式细胞周期检测β-岚香酮酸对HCC细胞周期的影响;利用Western blot检测凋亡相关蛋白Bcl2、BAX、cleaved-caspase 3和细胞周期相关蛋白CDK1、Cyclin B1的表达。结合网络药理学预测β-EA抑制HCC细胞增殖的分子机制,检测PI3K/AKT信号通路蛋白的表达。通过构建H22皮下移植瘤模型,体内证实了β-EA对HCC的生长抑制作用。结果表明,β-EA能够显著抑制Huh7和PLC/PRF/5细胞活力,诱导HCC细胞凋亡和G2/M期细胞阻滞,上调促凋亡蛋白cleaved-caspase 3、BAX,下调抗凋亡蛋白Bcl2、细胞周期蛋白Cyclin B1、细胞周期依赖性激酶CDK1、p-PI3K、p-AKT的表达。皮下移植瘤模型证实β-EA能够有效降低瘤重、瘤体积和Ki67的表达。本研究结果表明β-EA能够通过PI3K/AKT信号通路诱导HCC细胞凋亡和G2/M期细胞阻滞。

关键词：肝细胞癌 β-岚香酮酸细胞增殖细胞凋亡细胞周期阻滞

基于秀丽隐杆线虫模型的棉子糖抗衰老作用研究

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药物评价研究 2024年第2期47卷 339-344页

作者：高雅婷陈旭范潇晓郑天宇刘永刚张园园北京中医药大学中药学院北京100029

目的研究棉子糖抗衰老活性并探讨其作用机制。方法棉子糖(150、200、250μmol·L^(-1))加到大肠杆菌中孵育秀丽隐杆线虫后,显微镜下观察线虫身体弯曲频率、头尾摆动频率、体长,观察热应激刺激下线虫的存活时间;通过脂褐素荧光强度检... 详细信息

目的研究棉子糖抗衰老活性并探讨其作用机制。方法棉子糖(150、200、250μmol·L^(-1))加到大肠杆菌中孵育秀丽隐杆线虫后,显微镜下观察线虫身体弯曲频率、头尾摆动频率、体长,观察热应激刺激下线虫的存活时间;通过脂褐素荧光强度检测评价棉子糖对线虫抗衰老能力的影响;采用DAF-16核定位测定和超氧化物歧化酶-3(SOD-3)表达测定研究其抗衰老的相关机制。结果与对照组比较,棉子糖3个浓度均使线虫的身体弯曲频率加快(P<0.001);200μmol·L^(-1)棉子糖使线虫头尾摆动频率加快(P<0.01);体长测试结果显示棉子糖3个浓度均对线虫生长发育无毒害作用。热应激测试中,与对照组比较,棉子糖3个浓度均显著延长线虫在热应激条件下的存活时间(P<0.05)。脂褐素测试中,与对照组比较,棉子糖3个浓度均使线虫体内脂褐素含量显著降低(P<0.01)。DAF-16核定位测定中,棉子糖3个浓度均促进DAF-16蛋白入核表达。SOD-3表达测定中,与对照组比较,棉子糖250μmol·L^(-1)浓度组的SOD-3蛋白表达水平显著上升(P<0.05),与DAF-16核定位测定一并说明棉子糖抗衰老活性依赖ⅡS分子信号通路。结论棉子糖能够促进线虫的活动能力,对其生长发育无毒害性,并能延缓线虫的衰老速度,促进DAF-16蛋白入核表达,提高SOD-3蛋白表达水平,其抗衰老机制依赖ⅡS分子信号通路。

关键词：棉子糖秀丽隐杆线虫脂褐素抗衰老 DAF-16 超氧化物歧化酶-3

芪玉三龙方通过CBS/GPX4调控铁死亡促进肺癌移植瘤小鼠对顺铂的敏感性

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中华中医药杂志 2024年第5期39卷 2343-2348页

作者：刘桐张波克张星星高雅婷童佳兵李泽庚安徽中医药大学合肥230038 安徽中医药大学第一附属医院合肥230031 安徽省重点实验室中医药防治肺系重大疾病应用转化合肥230031

目的:基于生物信息技术和动物实验,探究芪玉三龙方提高肺癌顺铂敏感性的潜在作用机制。方法:分析转录组数据得到肺癌和顺铂耐药的差异基因,采用人工智能策略预测芪玉三龙方的靶蛋白,对取交集获得的潜在作用靶点进行GO生物过程及KEGG通... 详细信息

目的:基于生物信息技术和动物实验,探究芪玉三龙方提高肺癌顺铂敏感性的潜在作用机制。方法:分析转录组数据得到肺癌和顺铂耐药的差异基因,采用人工智能策略预测芪玉三龙方的靶蛋白,对取交集获得的潜在作用靶点进行GO生物过程及KEGG通路富集分析,随后进行基因依赖分析和分子对接,确定关键标志物,最后构建肺癌A549/DDP皮下瘤模型进行验证。结果:获得芪玉三龙方与肺癌顺铂耐药相关的潜在有效靶点186个,富集的生物学过程有细胞对过氧化氢的反应、细胞氧化解毒;功能通路有谷胱甘肽代谢、铂类药物的耐药性等。基因依赖分析鉴定出与铁死亡相关的基因CBS为核心的靶点,分子对接显示CBS与芪玉三龙方的核心活性成分有良好的亲和力,与贝母辛的结合亲和力最高。动物实验结果显示芪玉三龙方联合顺铂组肿瘤组织可见大片状坏死的细胞。与顺铂组比较,联合组能够上调4HNE和TFR蛋白的表达,下调CBS和GPX4蛋白的表达。结论:芪玉三龙方能够提高肺癌的顺铂耐药肿瘤组织对于顺铂的敏感性,其作用机制可能与抑制铁死亡关键分子CBS、GPX4表达有关。

关键词：芪玉三龙方肺癌 A549/DDP细胞铁死亡 CBS

Tuned selectivity and enhanced activity of CO_(2) methanation over Ru catalysts by modified metal-carbonate interfaces

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Journal of Energy Chemistry 2022年第1期31卷 38-46,I0002页

作者： Qiaojuan Wang yating gao Chantsalmaa Tumurbaatar Tungalagtamir Bold Fei Wei Yihu Dai Yanhui Yang Institute of Advanced Synthesis School of Chemistry and Molecular EngineeringNanjing Tech UniversityNanjing 211816JiangsuChina School of Applied Sciences Mongolian University of Science and TechnologyUlaanbaatar 14191Mongolia Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical EngineeringTsinghua UniversityBeijing 100084China

Carbonate-modified metal-support interfaces allow Ru/MnCO_(3) catalyst to exhibit over 99% selectivity,great specific activity and long-term anti-CO poisoning stability in atmospheric CO_(2) *** a contrast,Ru/MnO catalyst with metal-oxide interfaces prefers reverse water-gas shift rather than methanation route,along with a remarkably lower activity and a less than 15% CH_(4) *** carbonatemodified interfaces are found to stabilize the Ru species and activate CO_(2) and H_(2) ***-CO^(4) species are identified as the reaction intermediates steadily formed from CO_(2) dissociation,which show moderate adsorption strength and high reactivity in further hydrogenation to CH_(4),Furthermore,carbonates of Ru/MnCO_(3) are found to be consumed by hydrogenation to form CH_(4) and replenished by exchange with CO_(2),which are in a dynamic equilibrium during the *** with surface carbonates is proved as an efficient strategy to endow metal-support interfaces of Ru-based catalysts with unique catalytic functions for selective CO_(2) hydrogenation.

关键词： C0_(2)methanation Ru catalyst MnO Carbonate Metal-support interface

Encrypted traffic classification based on fusion of vision transformer and temporal features

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The Journal of China Universities of Posts and Telecommunications 2023年第2期30卷 73-82页

作者： Wang Lanting Hu Wei Liu Jianyi Pang Jin gao yating Xue Jingyao Zhang Jie School of Cyberspace Security Beijing University of Posts and TelecommunicationsBeijing 100876China State Grid Information and Telecommunication Branch Beijing 100761China Information and Telecommunication Company State Grid Shandong Electric Power CorporationJinan 250001China

Aiming at the problem that the current encrypted traffic classification methods only use the single network framework such as convolutional neural network(CNN),recurrent neural network(RNN),and stacked autoencoder(SAE),and only construct a shallow network to extract features,which leads to the low accuracy of encrypted traffic classification,an encrypted traffic classification framework based on the fusion of vision transformer and temporal features was *** transformer network(BoTNet)was used to extract spatial features and bi-directional long short-term memory(BiLSTM)was used to extract temporal *** the two sub-networks are parallelized,the feature fusion method of early fusion was used in the framework to perform feature ***,the encrypted traffic was identified through the fused *** experimental results show that the BiLSTM and BoTNet fusion transformer(BTFT)model can enhance the performance of encrypted traffic classification by fusing multi-dimensional *** accuracy rate of a virtual private network(VPN)and non-VPN binary classification is 99.9%,and the accuracy rate of fine-grained encrypted traffic twelve-classification can also reach 97%.

关键词： encrypted traffic classification vision transformer temporal feature

芪玉三龙方通过Wnt5a/β-catenin信号通路逆转上皮—间充质转化抑制肺癌细胞侵袭和转移

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安徽中医药大学学报 2024年第2期43卷 81-89页

作者：张裕童佳兵李泽庚高雅婷安徽中医药大学第二附属医院安徽合肥230061 安徽中医药大学第一附属医院呼吸科安徽合肥230031 安徽省教育厅重点实验室中医药防治肺系重大疾病重点实验室安徽合肥230031 新安医学教育部重点实验室安徽合肥230038

目的从细胞水平探讨芪玉三龙方是否通过Wnt5a/β-catenin信号通路抑制上皮—间充质细胞转化,抑制肺癌转移。方法将重组慢病毒转染小鼠肺癌细胞建立稳定过表达Wnt5a的细胞系,同时构建Wnt5a干扰和空载细胞株。qRT-PCR法检测Wnt5a、β-cate... 详细信息

目的从细胞水平探讨芪玉三龙方是否通过Wnt5a/β-catenin信号通路抑制上皮—间充质细胞转化,抑制肺癌转移。方法将重组慢病毒转染小鼠肺癌细胞建立稳定过表达Wnt5a的细胞系,同时构建Wnt5a干扰和空载细胞株。qRT-PCR法检测Wnt5a、β-catenin mRNA的表达水平。CCK8、EdU实验检测肺癌细胞的增殖活性,Transwell实验检测肺癌细胞的迁移和侵袭能力。Western blot法检测Wnt5a、β-catenin、c-Myc、Cyclin D1、Vimentin、N-cadherin、E-cadherin、Snail蛋白表达水平。结果与对照组比较,芪玉三龙方组Wnt5a、β-catenin、c-Myc、Cyclin D1蛋白表达水平下降(P<0.05),细胞增殖活性、侵袭和迁移能力显著降低(P<0.05)。Wnt5a过表达组中Vimentin、N-cadherin、Snail表达水平增加(P<0.05),E-cadherin蛋白表达水平下降(P<0.05),细胞增殖活性、侵袭和迁移能力显著增强(P<0.05);Wnt5a干扰组Vimentin、N-cadherin、Snail表达水平下降(P<0.05),E-cadherin蛋白表达水平升高(P<0.05),细胞增殖活性、侵袭和迁移能力显著下降(P<0.05),明显抑制EMT的发生。芪玉三龙方处理后,Wnt5a过表达组Wnt5a、β-catenin、c-Myc、Cyclin D1、Vimentin、N-cadherin、Snail表达水平下降(P<0.05),E-cadherin蛋白表达水平升高(P<0.05),细胞增殖活性、侵袭和迁移能力得到抑制。结论芪玉三龙方可能通过Wnt5a/β-catenin信号通路逆转上皮—间充质转化,抑制肺癌细胞增殖和转移。

关键词：芪玉三龙方 Wnt5a β-catenin 信号通路上皮—间充质转化增殖迁移

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周彩云基于“脾虚不敛”论治皮肌炎

中医学报 2024年第6期39卷 1271-1275页

作者：徐珊琼王鑫高娅婷傅俞杰张广德北京中医药大学研究生院北京100029 中国中医科学院西苑医院北京100091

皮肌炎的发生源于营卫不敛,营卫之气又源于脾胃化生,故脾虚可致卫气不固,邪气侵入,发而为病。病位在脾在肌,在肺在皮,后期多及于肾。临床诊治从脾虚不敛出发,针对出现的肌肉和皮肤症状,分别从“脾主肌肉”“肺主皮毛”论治,重在脾肺,诊... 详细信息

皮肌炎的发生源于营卫不敛,营卫之气又源于脾胃化生,故脾虚可致卫气不固,邪气侵入,发而为病。病位在脾在肌,在肺在皮,后期多及于肾。临床诊治从脾虚不敛出发,针对出现的肌肉和皮肤症状,分别从“脾主肌肉”“肺主皮毛”论治,重在脾肺,诊治过程中健脾益气贯穿始终,以四君子汤为基础方,兼生津润肺;湿、热、毒是皮肌炎病程进展的主要病理因素,常佐化湿、清热、解毒药味;善用甘药,以入脾胃;基于阳明在治疗痿证过程中的重要性,常用入阳明经的药物,以除阳明胃热、痰浊、湿热、食积、腑实,补阳明之气与阴。

关键词：皮肌炎脾虚不敛健脾益气生津润肺清热解毒四君子汤周彩云