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微小RNA-4463对人瘢痕疙瘩成纤维细胞增殖和侵袭的影响

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安徽医药 2021年第6期25卷 1140-1143,I0004页

作者：高栋梁张雷张宏峰杨喜明延安大学附属医院烧伤整形手外科陕西延安716000

目的研究微小RNA-4463(miR-4463)对人瘢痕疙瘩成纤维细胞增殖、迁移、侵袭的影响以及机制。方法收集延安大学附属医院2017年12月至2019年6月整形手术病人40例,每例切除瘢痕疙瘩组织1个以及邻近正常皮肤组织1个,利用定量聚合酶链反应(qP... 详细信息

目的研究微小RNA-4463(miR-4463)对人瘢痕疙瘩成纤维细胞增殖、迁移、侵袭的影响以及机制。方法收集延安大学附属医院2017年12月至2019年6月整形手术病人40例,每例切除瘢痕疙瘩组织1个以及邻近正常皮肤组织1个,利用定量聚合酶链反应(qPCR)检测人正常成纤维细胞和瘢痕疙瘩成纤维细胞中miR-4463的表达量;将miR-4463模拟物对照质粒和miR-4463模拟物分别在Lipofectamine 2000介导下转染入瘢痕疙瘩成纤维细胞,分为miR-NC组和miR-4463组;常规培养的瘢痕疙瘩成纤维细胞作为对照;qPCR检测转染后miR-4463的表达量;qPCR检测上调miR-4463对瘢痕疙瘩纤维化相关基因Col1 A1、Col 3 A1表达的影响;细胞计数试剂盒(CCK-8)检测上调miR-4463对瘢痕疙瘩成纤维细胞增殖的影响,Transwell实验检测细胞的迁移、侵袭;采用TargetScan软件预测miR-4463与B细胞淋巴瘤-2(Bcl-2)相关的致病基因BAG4的靶向关系,双荧光素酶报告基因进一步进行验证。结果miR-4463在瘢痕疙瘩成纤维细胞中的表达量较人正常成纤维细胞显著降低[(0.218±0.036)比(1.000±0.071),P<0.05];与对照组相比,转染miR-4463模拟物明显增加瘢痕疙瘩成纤维细胞中miR-4463的表达量[(1.000±0.073)比(3.313±0.242),P<0.05];上调miR-4463显著抑制瘢痕疙瘩纤维化相关基因Col 1 A1[(1.000±0.065)比(0.334±0.010)]、Col 3 A1[(1.000±0.070)比(0.301±0.008)]的表达量(P<0.05);上调miR-4463抑制瘢痕疙瘩成纤维细胞增殖[(0.836±0.081)比(0.656±0.072),P<0.05],降低其迁移[(153.269±12.471)个比(82.363±7.254)个]、侵袭细胞数[(73.623±6.451)个比(36.459±3.235)个](P<0.05);BAG4是miR-4463下游靶基因。结论miR-4463可能通过调控BAG4抑制细胞外基质中纤维化相关胶原基因的表达,抑制瘢痕疙瘩成纤维细胞增殖、迁移、侵袭。

关键词：瘢痕疙瘩成纤维细胞 miR-4463 Bcl2相关致病基因4(BAG4)

体外膜氧合技术治疗儿童严重急性呼吸窘迫综合征的预后因素分析

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中华儿科杂志 2024年第7期62卷 661-668页

作者：何小钰程晔高恒妙陈应富许巍成怡冰杨子浩王义程东良陈伟明闫钢风张羿洪小杨陆国平国家儿童医学中心复旦大学附属儿科医院重症医学科上海201102 国家儿童医学中心首都医科大学附属北京儿童医院重症医学科北京100045 重庆医科大学附属儿童医院重症医学科重庆400014 中国医科大学附属盛京医院小儿重症监护病房沈阳110004 河南省儿童医院重症医学科郑州450012 浙江大学医学院附属儿童医院重症医学科杭州310003 西安交通大学附属儿童医院重症医学科西安710003 河南省人民医院儿童重症监护室郑州450000 国家儿童医学中心复旦大学附属儿科医院流行病学教研室上海201102 解放军总医院第七医学中心儿科北京100007

目的探索影响体外膜氧合(ECMO)技术治疗严重儿童急性呼吸窘迫综合征(ARDS)预后的因素。方法多中心前瞻性观察性研究。以中国9家主要儿童ECMO中心2018年1月至2022年12月收治的95例ECMO挽救性治疗的严重ARDS患儿为研究对象,记录其一般资... 详细信息

目的探索影响体外膜氧合(ECMO)技术治疗严重儿童急性呼吸窘迫综合征(ARDS)预后的因素。方法多中心前瞻性观察性研究。以中国9家主要儿童ECMO中心2018年1月至2022年12月收治的95例ECMO挽救性治疗的严重ARDS患儿为研究对象,记录其一般资料、疾病危重度和脏器功能、综合治疗情况以及预后,根据出院时的转归分为存活组和死亡组,应用t检验、χ^(2)检验、多因素Logistic回归和混合线性模型分析ECMO启动前基线情况和ECMO过程中连续监测的指标(小儿危重评分、血小板计数、白蛋白、纤维蛋白原等)与预后的关系。结果95例接受了ECMO治疗的严重ARDS患儿中男55例(58%)、女40例(42%),年龄36.9(0.5,72.0)月龄。12例患儿(13%)存在免疫缺陷。68例(72%)患儿采用静脉动脉模式,27例(28%)采用静脉静脉模式。95例患儿出院存活率为51%(48例),静脉动脉和静脉静脉模式分别为47%(32/68)和59%(16/27)。死亡组患儿比存活组免疫缺陷的例数更多,小儿危重评分、血小板计数、白蛋白、纤维蛋白原和氧合指数均更低,呼吸机驱动压(ΔP)和氧指数均更高,ECMO前ARDS持续时间更长(均P0.05)。高ΔP、高氧指数、低氧合指数和低白蛋白均是影响预后的高危因素(均P<0.05)。ΔP≥25 cmH_(2)O(1 cmH_(2)O=0.098 kPa)、氧合指数≤67 mmHg(1 mmHg=0.133 kPa)和氧指数≥35均是预测不良预后的阈值(均P<0.05)。死亡组和存活组的ΔP、氧合指数、氧指数在ECMO后24 h起差异均有统计学意义(均P<0.05),并且随着ECMO进程差异逐步扩大;血小板水平从ECMO后7 d开始差异有统计学意义(P<0.05),并逐渐扩大;血乳酸水平在ECMO应用前、后差异有统计学意义(P<0.05),并从ECMO后24 h逐步扩大。结论ECMO前高ΔP、高氧指数、低氧合指数、低白蛋白是影响ECMO治疗严重儿童ARDS预后的高危因素。ECMO过程中第24小时起ΔP、氧指数逐渐降低和氧合指数逐渐升高预示预后良好,而ECMO应用后乳酸逐步升高预示预后不良。

关键词：儿童急性呼吸窘迫综合征体外膜氧合作用预后

An aerospace bracket designed by thermo-elastic topology optimization and manufactured by additive manufacturing

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Chinese Journal of Aeronautics 2020年第4期33卷 1252-1259页

作者： Guanghui SHI Chengqi GUAN dongliang QUAN Dongtao WU Lei TANG Tong gao Beijing Aerospace Technology Institute Beijing 100074China State IJR Center of Aerospace Design and Additive Manufacturing Northwestern Polytechnical UniversityXi'an 710072China Institute of Intelligence Material and Structure Unmanned System TechnologiesNorthwestern Polytechnical UniversityXi'an 710072China

Combination of topology optimization and additive manufacturing technologies provides an effective approach for the development of light-weight and high-performance structures.A heavy-loaded aerospace bracket is designed by topology optimization and manufactured by additive manufacturing technology in this *** both mechanical forces and temperature loads,a formulation of thermo-elastic topology optimization is firstly proposed and the sensitivity analysis is derived in *** the procedure of numerical optimization design is presented and the final design is additively manufactured using Selective Laser Melting(SLM).The mass of the aerospace bracket is reduced by over 18%,benefiting from topology and size optimization,and the three constraints are satisfied as well in the final *** work indicates that the integration of thermo-elastic topology optimization and additive manufacturing technologies can be a rather powerful tool kit for the design of structures under thermal-mechanical loading.

关键词： Additive manufacturing Aerospace bracket Selective laser melting(SLM) Thermo-elastic Topology optimization

不同皮瓣修复方法治疗头面部皮肤缺损的效果分析

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实用临床医药杂志 2020年第22期24卷 84-86,96页

作者：池凯刘杨周水勇王银钰高栋梁河南省郑州市郑东诺亚一家医疗美容门诊部整形外科河南郑州450000 延安大学附属医院烧伤整形手外科陕西延安716000

目的比较不同皮瓣修复方法对头面部皮肤缺损的治疗效果。方法选取60例头面部皮肤缺损患者作为研究对象,均行皮瓣修复治疗,根据手术方案的不同将患者分成3组,A组(n=14)采用双侧推进皮瓣修复治疗,B组(n=21)采用A-T皮瓣修复治疗,C组(n=25)... 详细信息

目的比较不同皮瓣修复方法对头面部皮肤缺损的治疗效果。方法选取60例头面部皮肤缺损患者作为研究对象,均行皮瓣修复治疗,根据手术方案的不同将患者分成3组,A组(n=14)采用双侧推进皮瓣修复治疗,B组(n=21)采用A-T皮瓣修复治疗,C组(n=25)采用菱形皮瓣修复治疗,比较3组患者的缺损部位、并发症发生率,随访1年,比较3组的瘢痕情况。结果 3组缺损部位比较,差异有统计学意义(P 0.05);3组瘢痕颜色、弹性程度评分和瘢痕宽度比较,差异无统计学意义(P> 0.05);C组瘢痕平整度评分低于A组和B组,差异有统计学意义(P <0.05)。结论双侧推进皮瓣修复、A-T皮瓣修复、菱形皮瓣修复均可有效修复头面部皮肤缺损,其中菱形皮瓣修复的瘢痕平整度更佳。

关键词：头面部皮肤缺损皮瓣修复瘢痕双侧推进皮瓣修复 A-T皮瓣修复菱形皮瓣修复

Responses of the circulation and water mass in the Beibu Gulf to the seasonal forcing regimes

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Acta Oceanologica Sinica 2014年第7期33卷 1-11页

作者： gao Jingsong SHI Maochong CHEN Bo GUO Peifang ZHAO dongliang Ocean University of China Guangxi Key Laboratory of Marine Environmental Science Guangxi Academy of Sciences

In the past 20 a, the gulf-scale circulation in the Beibu Gulf has been commonly accepted to be driven by a wind stress or density gradient. However, using three sensitive experiments based on a three-dimensional baroclinic model that was verified by observations, the formation mechanisms were revealed： the circula- tion in the northern Beibu Gulf was triggered by the monsoon wind throughout a year; whereas the southern gulf circulation was driven by the monsoon wind and South China Sea （SCS） circulation in winter and sum- mer, respectively. The force of heat flux and tidal harmonics had a strong effect on the circulation strength and range, as well as the local circulation structures, but these factors did not influence the major circulation structure in the Beibu Gulf. On the other hand, the Beibu Gulf Cold Water Mass （BGCWM） would disappear without the force of heat flux because the seasonal thermocline layer was generated by the input of heat so that the vertical mixing between the upper hot water and lower cold water was blocked. In addition, the wind-induced cyclonic gyre in the northern gulf was favorable to the existence of the BGCWM. However, the coverage area of the BGCWM was increased slightly without the force of the tidal harmonics. When the model was driven by the monthly averaged surface forcing, the circulation structure was changed to some extent, and the coverage area of the BGCWM almost extended outwards 100%, implying the circulation and water mass in the Beibu Gulf had strong responses to the temporal resolution of the surface forces.

关键词： Princeton ocean model (POM) Beibu Gulf (Gulf of Tonkin) circulation cold water mass response

The influence of temperature on rare earth flotation with naphthyl hydroxamic acid

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Journal of Rare Earths 2018年第1期36卷 99-107页

作者： Mei Li Kai gao dongliang Zhang Haijun Duan Linlin Ma Lan Huang School of Materials Science and Engineering Beijing University of Chemical Technology Inner Mongolia Key Laboratory of Rare Earth Hydrometallurgy and Light Rare Earth Application Inner Mongolia University of Science and Technology School of Material and Metallurgy Inner Mongolia University of Science and Technology

The influence of temperature on the complex process of Bayan Obo rare earth （RE） ore flotation with a collector of naphthyl hydroxamic acid （LF8#） was investigated. Industrial test data shows that the grade and recovery of RE increase with the temperature. However, the proportion of bastnaesite in the bulk concentrate increases as the RE grade improves. Adsorption mechanism of LF8# on the surfaces of bastnaesite and monazite were confirmed via zeta potential, UV]Vis Spectrophotometer （UV]Vis）, Fourier transform infrared （FIR）, and X-ray photoelectron spectroscopy analyses （XPS）. Although the results indicate that the total amount of the LF8# adsorption on the surface of bastnaesite and monazite de- creases with increasing the temperature, the amount of stable adsorbed predominance of characteristic bonds （-C（~O）N-] from LF8# uptake on bastnaesite surfaces increases significantly at high tempera- tures. This conclusion indicates that the adsorption stability increases with increasing the temperature. For monazite, the amount of characteristic elements C and N in LF8# does not increase as the temper- ature increases on the mineral surface, but the proportion of characteristic bonds increases, which shows that the adsorption stability of LF8# on the surface of monazite also increases, but it is not as significant as bastnaesite, which may be one of the reasons that the floatability of bastnaesite is better than those of monazite. Pulp dispersion results show that the temperature improve the dispersions of both the gangue and RE minerals. This improved the flotation selectivity so that it favored RE minerals. The calculated bubble size distribution confirms that higher temperatures generate smaller bubbles, thereby increasing the bubble-particle collision probability and the recovery of RE minerals.

关键词： BastnaesiteMonaziteFlotationHydroxamic acidAdsorptionRare earths

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丹参川芎嗪对兔耳增生性瘢痕的抑制作用研究

中国药业 2018年第5期27卷 1-3页

作者：冯登超高栋梁白翠翠高东东武斌薛宏斌杨喜明延安大学附属医院烧伤整形手外科陕西延安716000

目的观察丹参川芎嗪注射液对兔耳增生性瘢痕的抑制作用。方法取日本大耳白兔16只,建立兔耳增生性瘢痕动物模型。待创面上皮化(伤后21 d)后将其随机分为4组,每组4只,对照组(A组)局部注射生理盐水,实验组(B,C,D组)分别局部注射低、中、高... 详细信息

目的观察丹参川芎嗪注射液对兔耳增生性瘢痕的抑制作用。方法取日本大耳白兔16只,建立兔耳增生性瘢痕动物模型。待创面上皮化(伤后21 d)后将其随机分为4组,每组4只,对照组(A组)局部注射生理盐水,实验组(B,C,D组)分别局部注射低、中、高质量浓度的丹参川芎嗪注射液。于伤后32 d和56 d测量瘢痕厚度,并常规做HE染色,氯胺-T法检测瘢痕组织中羟脯氨酸(HPr)的含量变化;免疫组化观察瘢痕组织中增殖细胞核抗原蛋白(PCNA)的表达及变化。结果实验组中瘢痕厚度、HPr的含量及PCNA的阳性表达率与药物质量浓度呈负相关(P<0.05),瘢痕组织中胶原含量和PCNA蛋白水平均明显减少,呈剂量效应关系,与对照组比较,差异有统计学意义(P<0.01),伤后32 d和56 d实验组各亚组间差异有统计学意义(P<0.01)。结论丹参川芎嗪注射液通过减少胶原的合成水平,减少PCNA蛋白的表达,从而抑制兔耳增生性瘢痕组织增生,可为治疗增生性瘢痕提供新选择。

关键词：丹参川芎嗪注射液增生性瘢痕增殖细胞核抗原兔耳

Phase change,micro-structure and reaction mechanism during high temperature roasting of high grade rare earth concentrate

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Journal of Rare Earths 2020年第10期38卷 1140-1150,I0005页

作者： Huihui Wang Mei Li dongliang Zhang Kai gao Jianfei Li Zongxi Weng Wei Xu Mining Research Institute Inner Mongolia University of Science&TechnologyBaotou 014010China Key Laboratory of Green Extraction&Efficient Utilization of Light Rare-Earth Resources Ministry of EducationBaotou 014010China

The deposit of Bayan Obo in Inner Mongolia is the world’s largest rare earth element(abbreviated as REE)*** exploration of the theory of mineral formation of Bayan Obo is an important foundation for mineralogical research,and is the scientific basis for mining,industrial beneficiation,smelting and extraction,and processing and *** the rapid development of science and technology,the demand for the utilization of rare earth elements is increasing,and the separation process between rare earth elements needs to be *** purpose of this paper is to provide high temperature experimental information for the formation and application of rare earth *** this end,the mineral evolution of high-grade rare earth concentrates with increasing temperature and the migration of rare earths at different stages and their reaction mechanisms were *** to thermogravimetric analysis and differential scanning calorimetry(TG-DSC),calcination was carried out at different temperature ranges,and the calcined products were characterized by X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FT-IR),scanning electron micro scope and energy dispers ive spectrometer(SEM-EDS)and other analytical *** re sults are shown in this process,the ra re earth phase is first converted into rare earth oxide and rare earth *** the temperature increases,Ca5(PO4)3 F and a large number of self-shaped spherical Ca-RE-OF and Ca-RE-PO4 particles are formed,and the separation of La and Ce elements is *** rding to the phase diagram analysis,the production of Ca5(PO4)3 F is due to the reaction of monazite and fluorite,and the phases CeF2 and Ce F3 are formed during the *** it reaches 1500℃,barium ferrite is produced and a new substance containing Ba2+is formed.

关键词： High grade concentrates High-temperature roasting Rare-earth phase Decomposed Mechanism

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铝青铜QAl9-4的性能分析

科技资讯 2013年第23期11卷 70-71页

作者：赵晓波张军韩兵奇王发元高栋梁中国石油大学(华东)机电装备教学实习总厂山东东营257061

铝青铜具有许多优良的性能,在机械行业中起着重要的作用。尤其含铝量约10%时机械强度最高,且能通过热处理进一步强化,故QAl9-4可得到较高的强度、硬度、耐磨性、冲击无火花等优良性能。该材料已在抽油井光杆密封装置和阀门上得到了广泛... 详细信息

铝青铜具有许多优良的性能,在机械行业中起着重要的作用。尤其含铝量约10%时机械强度最高,且能通过热处理进一步强化,故QAl9-4可得到较高的强度、硬度、耐磨性、冲击无火花等优良性能。该材料已在抽油井光杆密封装置和阀门上得到了广泛应用,结果表明,QAl9-4耐磨、耐腐蚀,且对光杆及其密封填料、阀杆等具有良好的保护作用,提高了产品的使用寿命。

关键词： QAl9-4 性能可加工性强化处理