检索结果-南通市图书馆

Dissecting the genetic basis of maize deep-sowing tolerance by combining association mapping and gene expression analysis

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2022年第5期21卷 1266-1277页

作者： yang Yue MA Yu-ting LIU yang-yang Demar LYLE LI Dong-dong wang Ping-xi XU Jia-liang ZHEN Si-han LU Jia-wen PENG Yun-ling CUI Yu FU Jun-jie DU Wan-li ZHANG Hong-wei wang Jian-hua Agronomy college Shenyang Agricultural UniversityShenyang 110161P.R.China National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Center for Seed science and Technology College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China Gansu Provincial Key Laboratory for Aridland Crop science/Agronomy {1. Gansu Agricultural UniversityLanzhou 730070P.R.China

Deep-sowing is an important method for avoiding drought stress in crop species,including *** candidate genes is the groundwork for investigating the molecular mechanism underlying maize deep-sowing *** study evaluated four traits(mesocotyl length at 1. and 20 cm planting depths and seedling emergence rate on days 6 and 1.)related to deep-sowing tolerance using a large maize population containing 386 inbred lines genotyped with 0.5 million high-quality single nucleotide polymorphisms(SNPs).The genomewide association study detected that 273 SNPs were in linkage disequilibrium(LD)with the genetic basis of maize deep-sowing *** RNA-sequencing analysis identified 1.44 and 2098 differentially expressed genes(DEGs)in two comparisons,which shared 281.*** comparing the genomic locations of the 273 SNPs with those of the 281.DEGs,we identified seven candidate genes,of which GRMZM2G1.9769 encoded a sucrose non-fermenting 1.kinase interactor-like ***2G1.9769 was selected as the candidate gene because its homologs in other plants were related to organ length,auxin,or light *** gene association mapping revealed that natural variations in GRMZM2G1.9769 were related to phenotypic variations in maize mesocotyl *** expression of GRMZM2G1.9769 was higher in deep-sowing tolerant inbred *** results suggest that GRMZM2G1.9769 is the most likely candidate *** study provides information on the deep-sowing tolerance of maize germplasms and identifies candidate genes,which would be useful for further research on maize deep-sowing tolerance.

关键词： maize mesocotyl length association mapping differentially expressed gene SNF1.kinase interactor-like protein

Crystal structures of the two membrane-proximal Ig-like domains(D3D4)of LILRB1.B2:alternative models for their involvement in peptide-HLA binding

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2013年第10期4卷 761-770页

作者： Gol Nam Yi Shi Myongchol Ryu Qihui wang Hao Song Jun Liu Jinghua Yan Jianxun Qi George F {1. CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of sciences Beijing 100049China Institute of Microbiology State Academy of SciencesPyongyangDemocratic People’s Republic of Korea Research Network of Immunity and Health(RNIH) Beijing Institutes of Life ScienceChinese Academy of SciencesBeijing 100101China

Leukocyte immunoglobulin-like receptors(LILRs),also called CD85s,ILTs,or LIRs,are important mediators of immune activation and tolerance that contain tandem immunoglobulin(Ig)-like *** are 1.(in addition to two pseudogenes)LILRs in total,two with two Ig-like domains(D1.2)and the remaining nine with four Ig-like domains(D1.2D3D4).Thus far,the structural features of the D1.2 domains of LILR proteins are well defi ned,but no structures for the D3D4 domains have been *** is a very important fi eld to be studied as it relates to the unknown functions of the D3D4 domains,as well as their relative orientation to the D1.2 domains on the cell ***,we report the crystal structures of the D3D4 domains of both LILRB1.and *** two Ig-like domains of both LILRB1.D3D4 and LILRB2-D3D4 are arranged at an acute angle(~60°)to form a bent struc-ture,resembling the structures of natural killer inhibitory *** on these two D3D4 domain structures and previously reported D1.2/HLA I complex structures,two alternative models of full-length(four Ig-like domains)LILR molecules bound to HLA I are proposed.

关键词： LILRs D3D4 HLA binding crystal structure

长江流域丘陵与平原2类典型城镇面源污染来源解析

在线全文

学校读者我要写书评

暂无评论

环境工程学报 2024年第2期18卷 513-522页

作者：林炳权安欣琪赵晨高雅弘刘宇轩钟寅齐飞邱斌王振北胡潜孙德智北京林业大学水体污染源控制技术北京市重点实验室北京100083 北京林业大学污染水体源控与生态修复技术北京市高等学校工程研究中心北京100083

在长江流域丘陵城镇重庆永川区和平原城镇常州金坛区分别选取各含合流制和分流制2种排水体制的4个区域作为研究对象,监测雨天4个区域的河流、沿河排口和下垫面径流相关数据,分别采用河流质量守恒法、PMF模型和地表径流质量守恒法解析2... 详细信息

在长江流域丘陵城镇重庆永川区和平原城镇常州金坛区分别选取各含合流制和分流制2种排水体制的4个区域作为研究对象,监测雨天4个区域的河流、沿河排口和下垫面径流相关数据,分别采用河流质量守恒法、PMF模型和地表径流质量守恒法解析2类城镇4个区域面源对河流污染贡献、沿河排口污染来源贡献和各下垫面污染贡献。结果表明,城镇面源对4个研究区域内河流的5项水质指标(SS、COD、NH3-N、TN和TP)均有一定程度的贡献,其贡献率与降雨等级、地区特征和排水体制有关;降雨期间,在2类城镇2种排水体制的沿河排口污染负荷来源中,地表径流是SS和COD负荷的主要来源,生活污水和管道沉积物是NH_(3)-N、TN和TP负荷的主要来源;受地形坡度和人口密度等因素影响,永川和金坛的主要产污下垫面不同,永川为小区道路,金坛为交通道路和屋顶。为提高上述研究区域面源污染的精细化管理,提出了针对性的控制措施。

关键词：丘陵城镇平原城镇城镇面源污染质量守恒法 PMF模型

Supramolecular protein glue to boost enzyme activity

在线全文

学校读者我要写书评

暂无评论

science{1. Materials 2019年第9期62卷 1341-1349页

作者： Yuna Shang Yue Liao Zhongju Ye Zhongyan wang Lehui Xiao Jie {1. Qigang wang Zhimou yang Key Laboratory of Bioactive Materials Ministry of Education State Key Laboratory of Medicinal Chemical Biology College of Life Sciences and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University Tianjin 300071 China School of Chemical science and Engineering Tongji University Shanghai 200092 China State Key Laboratory of Medicinal Chemical Biology College of Chemistry Nankai University Tianjin 300071 China

Proteins possess many biological ***, they can easily degrade or aggregate, thus losing their bioactivity. Therefore, it is very important to develop materials capable of interacting with proteins and forming nanostructures for protein storage and delivery. In this study,we serendipitously found a novel peptide-based supramolecular protein glue(Nap-GFFYK(γE)2-NH2, compound 1. that could co-assemble with proteins into nanofibers and hydrogels. We found that compound 1.rapidly folded into a β-sheet conformation upon contact with many proteins but not with polymers. Total internal reflection fluorescence microscopy(TIRFM) images clearly show the formation of co-assembled nanofibers by proteins and the peptide. The supramolecular protein glue could improve the dispersion of enzymes(lipase and lysozyme) and therefore enhance their catalytic activity,especially at high temperatures. More importantly, the supramolecular protein glue could co-assemble with two enzymes, glucose oxidase/horseradish peroxidase(GOx/HRP)and GOx/cytochrome c(cyt c), to form nanofibers that significantly enhanced the catalytic activity of tandem enzymatic reactions. We envisioned the great potential of our supramolecular protein glue for protein storage, delivery, and bioactivity manipulation.

关键词： protein glue coassemble β-sheet enzyme activity

基于活性炭‖Na_(0.44)MnO_(2)的低成本、高倍率和长寿命碱性钠离子电池电容器

在线全文

学校读者我要写书评

暂无评论

物理化学学报 2024年第2期40卷 87-88页

作者：薛晴李圣驿赵亚楠盛鹏徐丽李正曦张波李慧王博杨立滨曹余良陈重学北京智慧能源研究院北京102200 武汉大学化学与分子科学学院化学电源材料与技术湖北省重点实验室武汉430072 国网青海省电力公司经济技术研究院西宁810008 国网金华供电公司浙江金华321017 武汉大学动力与机械学院水力机械过渡过程教育部重点实验室武汉430072

水系钠离子电池电容器具有成本低、功率大、安全性好等优点,是下一代大规模储能系统的理想选择之一。本文采用Na_(0.44)MnO_(2)正极、活性炭(AC)负极、6mol·L^(-1.NaOH电解液和廉价的不锈钢集流体构建了可充电碱性钠离子电池电容器。由... 详细信息

水系钠离子电池电容器具有成本低、功率大、安全性好等优点,是下一代大规模储能系统的理想选择之一。本文采用Na_(0.44)MnO_(2)正极、活性炭(AC)负极、6mol·L^(-1.NaOH电解液和廉价的不锈钢集流体构建了可充电碱性钠离子电池电容器。由于Na_(0.44)MnO_(2)正极在碱性电解液中具有较高的过充耐受性,通过首次充电时的原位过充预活化过程可以解决半钠化Na_(0.44)MnO_(2)正极和AC负极初始库伦效率低的缺点。因此,AC‖Na_(0.44)MnO_(2)可充电碱性钠离子电池电容器具有优异的电化学性能,在功率密度为85 W·kg^(-1.时,能量密度达26.6 Wh·kg^(-1.,循环1.000次后容量保持率为89%。同时,在50℃的高温和-20℃的低温也具有良好的电化学性能。这些结果表明AC‖Na_(0.44)MnO_(2)可充电碱性钠离子电池电容器具备应用于大规模储能的潜力。

关键词：钠离子电池电容碱性电解液过充自保护低成本宽工作温程

Prevalence of gene mutations in a Chinese 46,XY disorders of sex development cohort detected by targeted next-generation sequencing

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2021年第1期23卷 69-73页

作者： Bing-Qing Yu Zhao-Xiang Liu Yin-Jie gao Xi {1. Jiang-Feng Mao Min Nie Xue-Yan Wu NHC Key Laboratory of Endocrinology Department of EndocrinologyPeking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100730China

46,XY disorders of sex development(DSD)is characterized by incomplete masculinization genitalia,with gonadal dysplasia and with/without the presence of Mullerian *** least 30 genes related to 46,XY DSD have been ***,the clinical phenotypes of patients with different gene mutations overlap,and accurate diagnosis relies on gene sequencing ***,this study aims to determine the prevalence of pathogenic mutations in a Chinese cohort with 46,XY DSD by the targeted nextgeneration sequencing(NGS)***-seven 46,XY DSD patients were enrolled from the Peking Union Medical college Hospital({1.,china).A total of fifty-four rare variants were identified in 60 patients with 46,XY *** incidence of these rare variants was approximately 69.0%(60/87).Twenty-five novel variants and 29 reported variants were *** on the American college of Medical Genetics and Genomics(ACMG)guidelines,thirty-three variants were classified as pathogenic or likely pathogenic variants and 21.variants were assessed as variants of uncertain *** overall diagnostic rate was about 42.5%based on the pathogenic and likely pathogenic *** receptor{AR),steroid 5-alpha-reductase 2(SRD5A2)and nuclear receptor subfamily 5 Group A member 1.NR5A1.gene variants were identified in 21.1. and 1. patients,*** incidence of these three gene variants was about 78.3%(47/60)in patients with rare *** is concluded that targeted NGS is an effective method to detect pathogenic mutations in 46,XY DSD patients and AR,SRD5A2,and NR5A1.genes were the most common pathogenic genes in our cohort.

关键词： 46,XY disorders of sex development mutations targeted next-generation sequencing

应用噬菌体肽库筛选能封阻霍乱噬菌体VP1.染菌细胞的寡肽

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

微生物学报 2003年第4期43卷 509-513页

作者：王多春阚飙高守一刘延清中国疾病预防控制中心传染病预防控制所卫生部医学分子细菌学重点实验室北京102206

Use Vibrio cholerae El Tor Typing Phage VP1.as ligand to screen phage-display random 7 amino acid peptide library, ELISA and inactivation experiment were used to identify positive clone. The ratio of output to input was increased after three rounds of screening. Pseudo-positive was decreased stepwise. It indicated the efficient enrichment. After three rounds of screening, 31. out of 360 phage clones were positive in ELISA, 1.clone shows blocking VP1.adsorbs to Vibrio cholerae by inactivation experiment. Sequencing result indicate that amino acid sequences are p245: LeuGlnGlnLysHisLeuLeu; p40,p55: GlnLeuIleMetIleArgHis; p69,p274 IleThrProArgAsnArgSer. Getting some peptides can mimick the VP1.receptor epitopes, one peptide can block VP1.transfection to host cell, it was a clue to study receptor’s structure and phage infectious mechanism to host cell.

关键词：噬菌体肽库霍乱噬菌体寡肽霍乱弧菌

在线全文

学校读者我要写书评

暂无评论

我国分娩镇痛相关网络科普教育资料现状分析

中华麻醉学杂志 2024年第7期44卷 850-855页

作者：杨丽娜王小杰芮燕张永谦汤思怡于东吴安石魏昌伟首都医科大学附属北京朝阳医院麻醉科北京100020 北京语言大学信息科学学院北京100083

目的分析我国分娩镇痛相关网络科普教育资料现状。方法根据搜索引擎用户习惯,检索并筛选分娩镇痛相关资料。由3位麻醉科医师对内容覆盖率、准确率进行评价,利用中文版患者教育资料印刷品评估工具(C-PEMAT-P)从医学文本角度对可理解性及... 详细信息

目的分析我国分娩镇痛相关网络科普教育资料现状。方法根据搜索引擎用户习惯,检索并筛选分娩镇痛相关资料。由3位麻醉科医师对内容覆盖率、准确率进行评价,利用中文版患者教育资料印刷品评估工具(C-PEMAT-P)从医学文本角度对可理解性及可操作性进行主观评估。采用组内相关系数(ICC)法对3位麻醉科医师的评价结果进行一致性分析。采用机器学习联合ChatGPT-4.0方法建立中文可读性分级模型,从中文文本角度对纳入资料的阅读难易程度进行客观评估。结果共检索到97篇网页资料,共21.资料纳入研究。分娩镇痛禁忌证的内容覆盖率仅62%(1./21.;准确率在90%以上的网页资料构成比为71.(1./21.;易于理解的网页资料构成比为81.(1./21.,具有指导意义的网页资料构成比为5%(1.21.。对于内容覆盖率、准确率、可理解性及可操作性一致性评价的ICC值分别为0.975、0.833、0.758和0.773(P1.。适合义务教育水平(可读性评分2分)读者的网页资料构成比为5%(1.21.,适合高中及以上教育水平(可读性评分为3分)读者的网页资料构成比为43%(9/21.。结论我国分娩镇痛相关网络科普教育资料数量多,但质量有待提高。未来应开展"麻醉学+语言学"合作模式,为患者提供内容更全面、准确更高、更适合孕妇理解的科普教育资料。

关键词：镇痛产科互联网使用科普信息质量

Toripalimab combined with lenvatinib and GEMOX is a promising regimen as first-line treatment for advanced intrahepatic cholangiocarcinoma:a single-center,single-arm,phase 2 study

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2023年第4期8卷 1983-1992页

作者： Guo-Ming Shi Xiao-Yong Huang Dong Wu Hui-Chuan Sun Fei Liang Yuan Ji Yi Chen Guo-Huan yang Jia-Cheng Lu Xian-Long Meng Xin-Ying {1. Lei Sun Ning-Ling Ge Xiao-Wu Huang Shuang-Jian Qiu Xin-Rong yang Qiang gao Yi-Feng He yang Xu Jian Sun Zheng-Gang Ren Jia Fan Jian Zhou Department of Liver Surgery and Transplantation Liver Cancer InstituteZhongshan HospitalFudan UniversityShanghaiChina Department of Radiology Zhongshan HospitalFudan UniversityShanghaiChina Department of Statistics Zhongshan HospitalFudan UniversityShanghaiChina Department of Pathology Zhongshan HospitalFudan UniversityShanghaiChina Department of Hepatic Oncology Liver Cancer InstituteZhongshan HospitalFudan UniversityShanghaiChina Tianjin Medical Laboratory BGI-TianjinBGI ShenzhenTianjinChina

Advanced intrahepatic cholangiocarcinoma(ICC)has a dismal ***,we report the efficacy and safety of combining toripalimab,lenvatinib,and gemcitabine plus oxaliplatin(GEMOX)as first-line therapy for advanced *** patients with pathologically confirmed advanced ICC received intravenous gemcitabine(1.g/m2)on Days 1.and 8 and oxaliplatin(85 mg/m2)Q3W for six cycles along with intravenous toripalimab(240 mg)Q3W and oral lenvatinib(8 mg)once daily for one *** expression of programmed death-ligand 1.PD-L1.and genetic status was investigated in paraffin-embedded tissues using immunohistochemistry and whole-exome sequencing(WES)*** primary endpoint was the objective response rate(ORR).Secondary outcomes included safety,overall survival(OS),progression-free survival(PFS),disease control rate(DCR)and duration of response(DoR).As of July 1.2022,the median follow-up time was 23.5 months,and the ORR was 80%.Twenty-three patients achieved partial response,and one achieved complete ***(21.30)with DNA damage response(DDR)-related gene mutations showed a higher ORR,while patients(1./30)with tumor area positivity≥1.PD-L1.staining)showed a trend of high ORR,but without significant *** median OS,PFS,and DoR were 22.5,1..2,and 1..0 months,*** DCR was 93.3%.Further,56.7%of patients experienced manageable grade≥3 adverse events(AEs),commonly neutropenia(40.0%)and leukocytopenia(23.3%).In conclusion,toripalimab plus lenvatinib and GEMOX are promising first-line regimens for the treatment of advanced ICC.A phase-III,multicenter,double-blinded,randomized study to validate our findings was approved by the National Medical Products Administration(NMPA,No.2021.P01.25).

关键词： regimen intrahepatic treatment