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Epistatic effect between ACACA and FABP2 gene on abdominal fat traits in broilers

Journal of Genetics and Genomics 2010年第8期37卷 505-512页

作者： Guo Hu 1. Shouzhi wang 1. Jianwei Tian, Lili Chu, Hui Li {1. of Animal science and Technology, Northeast agricultural university, Harbin 1.0030, china [a] college of Animal {1. and Technology Northeast Agricultural University Harbin 150030 China

Epistasis is generally defined as the interaction between two or more genes or their mRNA or protein products to influence a single trait. Experimental evidence suggested that epistasis could be important in the determination of the genetic architecture of complex traits in domestic animals. Acetyl-coenzyme A carboxylase alpha （ACACA） and fatty acid binding protein 2 （FABP2） are both key factors of lipogenesis and transport. They may play a crucial role in the weight variability of abdominal adipose tissue in the growing chicken. In this study, the polymorphisms of c.2292G〉A in A CA CA and c.-561.〉C in FABP2 were detected among individuals from two broiler lines which were divergently selected for abdominal fat content. Epistasis between the two SNPs on abdominal fat weight （AFW） and ab- dominal fat percentage （AFP） was analyzed. The additive x additive epistatic components between these two SNPs were found significant or suggestively significant on both AFW and AFP in lean lines of the 9th and 1.th generation; whereas, it was not significantly associated with either AFW or AFP in fat lines. At the same time, there were not any other significant epistatic components found in both generations or in both lines. Significant epistatic effects between these two SNPs found only in the lean lines could partly be due to the fact that the abdominal fat traits in these two experimental lines have been greatly modified by strong artificial selection. The results suggested that the epistasis mode may be different between the lean and fat chicken lines. Our results could be helpful in further understanding the genetic interaction between candidate genes contributing to phenotypic variation of abdominal fat content in broilers.

关键词： chicken fatness epistasis ACACA FABP2 QTL

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EST-derived microsatellites in P seudosciaena crocea and their applicability to related species

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Acta Oceanologica Sinica 2010年第6期29卷 83-91页

作者： YE Hua wang Xiaoqing {1. Tianxiang wang Zhiyong college of Animal {1. and Technology Hunan Agricultural University Changsha 410128 China Key Laboratory of science and Technology for Aquaculture and Food Safety Fisheries College/Fisheries Biotechnology Institute Jimei University Xiamen 361021 China Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 China

Simple sequence repeat （SSR） markers were obtained for the large yellow croaker Pseudosciaena crocea using 1.205 expressed sequences tags （ESTs） from the NCBI *** for 48 ESTSSR loci were designed and screened with 30 *** specimens captured from Guanjingyang sea area in Fujian Province of *** of the loci were polymorphic,which were amplified with 3 to 1. alleles per locus and the mean of *** observed and expected heterozygosity per locus ranged from 0.091.to 0.844 （mean 0.544） and from 0.1.8 to 0.892 （mean 0.644）,*** information content （PIC） ranged from 0.1.5 to 0.866 （mean 0.593）.The results for cross-species amplification of the 1. large yellow croaker EST-SSRs on ***,***,***,*** and *** revealed that 1.,1.,1.,7 and 6 loci were successfully amplified with 1.to 1. alleles with an average of 4.5 per locus,respectively,which are suitable for population genetics studies of these species and useful for phylogenetic relationship analysis among these ***,this study provides a set of type I markers for population genetics studies and genome mapping for large yellow croaker and its closely related species.

关键词： Pseudosciaena crocea EST-SSRs genetic diversity cross-species amplification

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Generation of fad2 transgenic mice that produce omega-6 fatty acids

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science {1.(Life sciences) 2009年第11期52卷 1048-1054页

作者： CHEN Qing1. LIU Qing2, WU ZhiFang1. wang ZongYi3 & GOU KeMian1.1.State Key Laboratory for Agrobiotechnology,{1., Beijing 1.01.3, china 2 Blackmountain Laboratory, CSIRO Plant Industry, Canberra, Australia 3 college of Animal science and Technology,{1., Beijing 1.01.3, china 1. State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100193 China 2. Blackmountain Laboratory CSIRO Plant Industry Canberra Australia 3. college of Animal {1. and Technology China Agricultural University Beijing 100193 China

Fatty acid desaturase-2 (FAD2) introduces a double bond in position 1. in oleic acid (1.:1. to form linoleic acid (1.:2 n-6) in higher plants and microbes. A new transgenic expression cassette, containing CMV promoter/fad2 cDNA/SV40 polyA, was constructedto produce transgenic mice. Among 63 healthy offspring, 1. founders (1..9%) integrated the cotton fad2 transgene into their genomes, as demonstrated by PCR and Southern blotting analysis. All founder mice were fertile and heterozygous fad2 female and nontransgenic littermates were used for fatty acid analysis using gas chromatography. One fad2 transgenic line showed substantial differences in the fatty acid profiles and the level of linoleic acid was increased 1.% (P20:4 n-6) in muscles and liver, compared to their nontransgenic littermates. The results indicate that the plant fad2 gene can be functionally expressed in transgenic mice and may playan active role in conversion of oleic acid into linoleic acid.

关键词： fatty acid desaturase FAD2 transgenic mouse oleic acid linoleic acid arachidonic acid

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Computational theory of cavitating flows for hydraulic turbomachinery with consideration of influence of water quality

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science {1.(Technological sciences) 2010年第12期53卷 3341-3348页

作者： wang Lei1.2 & CHANG JinShi2 1.Huadian Electric Power Research Institute, Hangzhou 31.030, {1. 2 college of Water Conservancy and Civil Engineering, china agricultural university, Beijing 1.0083. {1. 1. Huadian Electric Power Research Institute Hangzhou 310030 China2. College of Water Conservancy and Civil Engineering China Agricultural University Beijing 100083 China

Previously it was assumed that the pressure within the cavity or on the cavity surface remained constant and the vapor pressure of clean water at 20°C and 0 m altitude was utilized as the computational boundary for cavitating flows in hydraulic turbomachinery. Cavitation was confused with vaporization, and the effect of water quality on cavitation pressure characteristics was not taken into account. In recent years, lots of experiments of cavitation pressure characteristics of different water qualities including different sand concentrations of sand water and different altitudes of clean water have been performed by the authors, and the important influences of water quality on cavitation pressure characteristic have been validated. Thus the water quality should be involved in the cavitating flows computation. In the present paper, the effect of water quality on the cavitation pressure characteristic is analyzed and the computational method and theory of cavitating flows for hydraulic turbomachinery that considers the influence of water quality are proposed. The theory is suitable for both the potential flow method and the two-phase flow method for cavitating flows simulation. Finally, the validation results for cavitating flows in a hydraulic tur- bine indicate the significant influences of water quality on the cavitating flow performance.

关键词： cavitating flows cavitation pressure water quality hydraulic turbomachinery

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Inducement of chromosome translocation with small alien segments by irradiating mature female gametes of the whole arm translocation line

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science {1.(Life sciences) 2008年第4期51卷 346-352页

作者： CHEN ShengWei1.2, CHEN PeiDu1.& wang XiuE1.1.National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing {1. university, Nanjing 21.095, china 2 college of Agronomy and Biotechnology, Yunnan {1. university, Kunming 650201. china National Key Laboratory of Crop Genetics Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China college of Agronomy Biotechnology Yunnan Agricultural University Kunming 650201 China

Haynaldia villosa Schur. (syn. Dasypyrum villosum Candargy, 2n=1., VV) has been proved to be an important genetic resource for wheat improvement. The development of translocation with small alien chromosome segments, especially interstitial translocation, will be helpful for better utilization of its useful genes. Up to now, most of the reported Triticum aestivum – H. villosa translocation lines are involved in a whole arm or large alien fragments. In this paper, we report a highly efficient approach for the creation of small chromosome segment translocation lines. Before flowering, the female gametes of wheat-H. villosa 6VS/6AL translocation line were irradiated by 60CO-γ ray at 1.0 Rad/M dosage rate and three dosages (1.00, 1.20, 2240 Rad). Anthers were removed from the irradiated florets on the same day and the florets were pollinated with normal fresh pollens of T. aestivum cv. Chinese Spring after 2-3 days. Genomic in situ hybridization (GISH) at mitosis metaphase of root-tip cell of M1.plants was used to detect the chromosome structural changes involving 6VS of H. villosa. Among the 534 M1.plants screened, 97 plants contained small segment chromosome structural changes of 6VS, including 80 interstitial translocation chromosomes, 57 terminal translocation chromosomes and 55 deletion chromosomes. For the 2240 Rad dosage treatment, the inducement frequencies of interstitial translo-cation, terminal translocation and deletion were 21.02%, 1..01., and 1..65%, respectively, which were much higher than those previously reported. The M2 seeds were obtained by backcrossing of 74 M1.plants involving 1.6 chromosomes structural changes of 6VS, and it was found that the structural aberrations in the M1.plants could be transmitted to their progenies. Irradiating mature female gametes of whole arm translocation is a new and highly efficient approach for creation of small segment chromosome struc-tural changes, especially for interstitial translocations.

关键词： irradiation mature female gametes small fragment translocation chromosome wheat Haynaldia villosa genomic in situ hybridization

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Structural changes of cellobiohydrolase I (1.4-β-D-glucan-cellobiohydrolase I, CBHI) and PNPC (p-nitro-phenyl-β-D-cellobioside) during the binding process

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science {1.(Life sciences) 2008年第5期51卷 459-469页

作者： WU Bin1.2, wang LuShan1.& {1. PeiJi1.1.State Key Laboratory of Microbial Technology, Shandong university, Jinan 2501.0, china 2 Institute of Poultry science, Shandong Academy of agricultural sciences, Jinan 250023, china State Key Laboratory of Microbial Technology Shandong University Jinan 250100 China Institute of Poultry science Shandong Academy of Agricultural Sciences Jinan 250023 China

Conformational changes to 1.4-β-D-glucan cellobiohydrolase I (CBHI) in response to its binding with p-nitrophenyl β-D-cellobioside (PNPC) were analyzed by second-derivative fluorescence spectrometry at the saturation binding point. Irreversible changes to the configuration of PNPC during the course of the binding process were characterized by UV spectral analysis. Isothermal titration calorimetry (ITC) was used to determine the stoichiometry of binding (i.e. the number of molar binding sites) of PNPC to CBHI. Two points on the surface of the CBHI molecule interact with PNPC, and irreversible changes to the configuration of PNPC occur during its conversion to p-nitrophenyl (PNP). The ITC studies demonstrated that the binding of PNPC to CBHI is an irreversible process, in which heat is released, but where there is no reversible equilibrium between PNPC-CBHI and CBHI and PNPC. On the other hand, PNP and cellobiose need to be released from the PNPC-CBHI complex to facilitate the repeated binding of new PNPC molecules to the renewable CBHI molecules. Therefore, we speculate that the energy, which powers the configurational change of PNPC as it is converted to PNP, is generated from cyclic changes in the conformation of CBHI during the binding/de-sorption process. These new insights may provide a basis for a better understanding of the binding mechanism in enzyme-substrate interactions.

关键词： saturated binding point cellobiohydrolase I p-nitrophenyl β-D-cellobioside conformation/configuration

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Influence of Formate on Bioactivity Material-thuringiensin Synthesized by Bacillus thuringiensis YBT-032

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Journal of Wuhan university of Technology(Materials {1.) 2008年第5期23卷 691-693页

作者：王志喻子牛 college of Bioengineering Hubei University of Technology State Key Laboratoy of agricultural Microbiology National Engineering Research Center for Microbial Posticides Huazhong Agricultural University

The biological method to synthesize thuringiensin and the influence of formate on thuringiensin biosynthesis were investigated. Addition of 1.00 g/L formate to growth medium of bacillus thuringiensis YBT-032 resulted in significant enhancements in productions of citrate, α -ketoglutarate, intracellular adenine and thuringiensin. These results demonstrate that added formate attends metabolism of cell, facilitates carbon metabolic flux in tricarboxylic acid cycle and hexose monophosphate pathway. As a carbon source, formate facilitates cell growth, increases glucose consumption and enhances the ability of cell to synthesis adenine analogues, and subsequently thuringiensin. Thuringiensin production rate significantly enhanced from 6.44 to 8.46 mg·g^-1.· h and transformation ratio from glucose to thuringiensin increased by 43.30%.

关键词： bacillus thuringiensis precursor formate thuringiensin

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Study on NDVI-T_s space by combining LAI and evapotranspiration

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science {1. Earth sciences 2006年第7期49卷 747-754页

作者： HAN Lijuan1,2,3, wang Pengxin4, yang Hua1,2,3, LIU Shaomin1,2,3 & wang Jindi1,2,3 1. Research Center for Remote Sensing and GIS, School of Geography, Beijing Normal university, Beijing 100875, china 2. State Key Laboratory of Remote Sensing science, Beijing 100875, china 3. Beijing Key Laboratory for Remote Sensing of Environment and Digital Cities, Beijing 100875, china 4. Department of Information Management, college of Information and Electronic Engineering, china agricultural university, Beijing 1.0083. china 1. Research Center for Remote Sensing and GIS School of Geography Beijing Normal University Beijing 100875 China 2. State Key Laboratory of Remote Sensing science Beijing 100875 China 3. Beijing Key Laboratory for Remote Sensing of Environment and Digital Cities Beijing 100875 China 4. Department of Information Management College of Information and Electronic Engineering China Agricultural University Beijing 100083 China

This paper focuses on interpreting the different spatial relationships between NDVI and Ts, a triangular or a trapezoid, and on analyzing transformation conditions, the physical and ecological meanings of the vegetation index-surface temperature space as well. Further, we use the Tempera-ture-Vegetation Dryness Index (TVDI) to explain the existent meaning of a triangular space after NDVI reaches its saturated state by employing the relationships between NDVI, LAI and evapotranspiration. The specific relations between NDVI and Ts are useful for describing, validating and updating land surface models.

关键词： NDVI, land surface temperature, evapotranspiration, leave area index.

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Development of a Chinese land data assimilation system:its progress and prospects

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Progress in Natural science:Materials International 2007年第8期17卷 881-892页

作者： Li Xin~(1.*) Huang Chunlin~1.Che Tao~1.Jin Rui~1.wang Shugong~l wang Jiemin~1.gao Feng~1.Zhang Shuwen~2 Qiu Chongjian~2 wang Chenghai~2 (1.Cold and Arid Regions Environmental and Engineering Research Institute,Chinese Academy of sciences,Lanzhou 730000,china 2.college of Atmospheric sciences,Lanzhou university,Lanzhou 730000,china) [a]Cold and Arid Regions Environmental and Engineering Research Institute Chinese Academy of Sciences Lanzhou China [b]college of Atmospheric {1.s Lanzhou University Lanzhou China

The objective of land data assimilation is to merge multi-source observations into the dynamics of land surface model for improving the estimation of land surface *** have developed a land data assimilation system for china's land *** this sys- tem,the Common Land Model and Simple Biosphere Model 2 are used to simulate land surface *** radiative transfer models of thawed and frozen soil,snow,lake,and vegetation are used as observation operators to transfer model predictions into estimated bright- ness temperatures.A Monte-Carlo based sequential filter,the ensemble Kalman filter,is implemented as data assimilation method to inte- grate modeling and *** system is capable of assimilating passive microwave remotely sensed data such as special sensor mi- crowave/imager (SSM/I),TRMM microwave imager (TMI),and advanced microwave scanning radiometer enhanced for EOS (AMSR- E) and the conventional in situ measurements of soil and snow.A spatiotemporally consistent assimilated dataset for soil moisture,soil temperature,snow and frozen soil,with a spatial resolution of 0.25 degree and temporal resolution of one hour,has been *** paper introduces the development of Chinese land data assimilation system and the progress made on data assimilation algorithms,land sur- face modeling,microwave remote sensing of land surface hydrological variables,and the preparation of atmospheric forcing *** dis- tinct characteristics and challenges of developing land data assimilation system and the perspectives for future development are also dis- cussed.

关键词： land data assimilation land surface model passive microwave remote sensing Kalman filter

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The CXXC finger 5 protein is required for DNA damage-induced p53 activation

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science {1.(Life sciences) 2009年第6期52卷 528-538页

作者： ZHANG Min1.wang RuiPeng1.wang YanYi 2,DIAO FeiCi1.LU Fei1.gao Dong1.CHEN DanYing 1.ZHAI ZhongHe1.& SHU HongBing2 1.college of Life sciences,Peking university,Beijing 1.0871.china 2 college of Life sciences,Wuhan university,Wuhan 430072,china college of Life {1.s Peking University Beijing 100871 China college of Life {1.s Wuhan University Wuhan 430072 China

The tumor suppressor p53 is a critical component of the DNA damage response pathway that induces a set of genes responsible for cell cycle arrest,senescence,apoptosis,and DNA *** ataxia te-langiectasia mutated protein kinase(ATM) responds to DNA-damage stimuli and signals p53 stabiliza-tion and activation,thereby facilitating transactivation of p53 inducible genes and maintainence of genome *** this study,we identified a CXXC zinc finger domain containing protein termed CF5 as a critical component in the DNA damage signaling ***5 induces p53 transcriptional activity and apoptosis in cells expressing wild type p53 but not in p53-deficient *** of CF5 in-hibits DNA damage-induced p53 activation as well as cell cycle ***,CF5 physically interacts with ATM and is required for DNA damage-induced ATM phosphorylation but not its recruitment to *** findings suggest that CF5 plays a crucial role in ATM-p53 signaling in response to DNA damage.

关键词： p53 ATM DNA damage apoptosis

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