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Nonconserved epitopes dominate reverse preexisting T cell immunity in COVID-19 convalescents

Signal Transduction and Targeted Therapy 2024年第7期9卷 2984-2998页

作者： Xin wang Jie Zhang Maoshun Liu Yuanyuan Guo Peipei Guo Xiaonan yang Bingli Shang Min Li Jinmin Tian Ting Zhang Xi wang Ronghua Jin Jikun Zhou George F.gao Jun Liu Department of Epidemiology School of Public HealthCheeloo College of MedicineShandong UniversityJinan 250012China NHC Key Laboratory of Biosafety Research Unit of Adaptive Evolution and Control of Emerging VirusesChinese Academy of Medical SciencesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention(China CDC)Beijing 102206China beijing Key Laboratory of Emerging Infectious Diseases Institute of Infectious DiseasesBeijing Ditan HospitalCapital Medical UniversityBeijing 100015China beijing Institute of Infectious Diseases Beijing 100015China National Center for Infectious Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015P.R.China School of Laboratory Medicine and Life sciences Wenzhou Medical UniversityWenzhou 325035China School of Ophthalmology&Optometry Wenzhou Medical UniversityWenzhou 325027China Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry Guangxi Medical UniversityNanningGuangxi 530021China Shijiazhuang Fifth Hospital Shijiazhuang 050011China CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences(CAS)Beijing 100101China Savaid Medical School University of Chinese Academy of SciencesBeijing 100049China

The herd immunity against SARS-CoV-2 is continuously consolidated across the world during the ongoing ***,the potential function of the nonconserved epitopes in the reverse preexisting cross-reactivity induced by SARS-CoV-2 to other human coronaviruses is not well *** our research,we assessed T cell responses to both conserved and nonconserved peptides shared by SARS-CoV-2 and SARS-CoV,identifying cross-reactive CD8^(+)T cell epitopes using enzyme-linked immunospot and intracellular cytokine staining ***,in vitro refolding and circular dichroism were performed to evaluate the thermal stability of the HLA/peptide ***,single-cell T cell receptor reservoir was analyzed based on tetramer ***,we discovered that cross-reactive T cells targeting SARS-CoV were present in individuals who had recovered from COVID-19,and identified SARS-CoV-2 CD8^(+)T cell epitopes spanning the major structural antigens.T cell responses induced by the nonconserved peptides between SARS-CoV-2 and SARS-CoV were higher and played a dominant role in the cross-reactivity in COVID-19 ***-T cell reactivity was also observed within the identified series of CD8^(+)T cell *** representative immunodominant peptide pairs,although the HLA binding capacities for peptides from SARS-CoV-2 and SARS-CoV were similar,the TCR repertoires recognizing these peptides were *** results could provide beneficial information for the development of peptide-based universal vaccines against coronaviruses.

关键词： immunity conserved assessed

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Regulation effects of water and nitrogen on yield,water,and nitrogen use efficiency of wolfberry

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Journal of Arid Land 2024年第1期16卷 29-45页

作者： gao Yalin QI Guangping MA Yanlin YIN Minhua wang Jinghai wang Chen TIAN Rongrong XIAO Feng LU Qiang wang Jianjun college of Water Conservancy and Hydropower Engineering Gansu Agricultural UniversityLanzhou 730070China Jingtaichuan Electric Power Irrigation Water Resource Utilization Center in Gansu Province Baiyin 730900China

Wolfberry(Lycium barbarum L.)is important for health care and ecological ***,it faces problems of low productivity and resource utilization during *** reasonable models for water and nitrogen management is important for solving these *** on field trials in 2021 and 2022,this study analyzed the effects of controlling soil water and nitrogen application levels on wolfberry height,stem diameter,crown width,yield,and water(WUE)and nitrogen use efficiency(NUE).The upper and lower limits of soil water were controlled by the percentage of soil water content to field water capacity(θ_(f)),and four water levels,i.e.,adequate irrigation(W0,75%-85%θ_(f)),mild water deficit(W1,65%-75%θ_(f)),moderate water deficit(W2,55%-65%θ_(f)),and severe water deficit(W3,45%-55%θ_(f))were used,and three nitrogen application levels,i.e.,no nitrogen(N0,0 kg/hm^(2)),low nitrogen(N1,150 kg/hm^(2)),medium nitrogen(N2,300 kg/hm^(2)),and high nitrogen(N3,450 kg/hm^(2))were *** results showed that irrigation and nitrogen application significantly affected plant height,stem diameter,and crown width of wolfberry at different growth stages(P1),and their maximum values were observed in W1N2,W0N2,and W1N3 *** weight per plant and yield of wolfberry first increased and then decreased with increasing nitrogen application under the same water *** weight per hundred grains and dry weight percentage increased with increasing nitrogen application under W0 ***,under other water treatments,the values first increased and then decreased with increasing nitrogen *** and its component of wolfberry first increased and then decreased as water deficit increased under the same nitrogen *** water use efficiency(IWUE,8.46 kg/(hm^(2)·mm)),WUE(6.83 kg/(hm^(2)·mm)),partial factor productivity of nitrogen(PFPN,2.56 kg/kg),and NUE(14.29 kg/kg)reached their highest values in W2N2,W1N2,W1N2,and W1N1 treatments.R

关键词： water deficit growth characteristics yield water and nitrogen use efficiency principal component analysis

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Cloning, sequencing and phylogenic analysis of duck prion gene

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Progress in Natural science:Materials International 2004年第9期 93-96页

作者： wang Qigui 1,2 *, ZHANG Lei 1*, HU Xiaoxiang 1, FAN Baoliang 1, LI Ning 1**, LI Hui 2 and WU Changxin 3(1. State Key Laboratory of Agrobiotechnology, china agricultural university, beijing 100094, china 2. college of Animal science and Technology, Northeast agricultural university, Harbin 150030, china 3. college of Animal science and Technology, china agricultural university, beijing 100094, china) State Key Laboratory of Agrobiotechnology，china agricultural university，beijing 100094，china college of Animal science and Technology，Northeast agricultural university，Harbin 150030，china State Key Laboratory of Agrobiotechnology，china agricultural university，beijing 100094，china college of Animal science and Technology，Northeast agricultural university，Harbin 150030，china college of Animal science and Technology，china agricultural university，beijing 100094，china

Duck prion gene was cloned and sequenced. Similar to mammalian prion protein (PrP), duck prion is encoded by a single exon of a single copy in genome, which was confirmed by Southern blot analysis. All of the structural features of mammalian PrP were also identified in the duck PrP. Compared with mammalian PrP, it exhibited a 30% of general similarity. When compared with chicken PrP, it showed a higher homology of 97%. A phylogenetic tree was constructed to trace evolution of prion gene in animals.

关键词： duck prion PrP cloning and sequencing phylogenetic tree

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Effects of thinning and understory removal on water use efficiency of Pinus massoniana:evidence from photosynthetic capacity and stable carbon isotope analyses

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Journal of Forestry Research 2024年第2期35卷 42-53页

作者： Ting wang Qing Xu Beibei Zhang Deqiang gao Ying Zhang Jing Jiang Haijun Zuo Key Laboratory of Forest Ecology and Environment of National Forestry and Grassland Administration Ecology and Nature Conservation InstituteChinese Academy of ForestryBeijing 100091People’s Republic of China college of Agronomy and agricultural Engineering Liaocheng UniversityLiaocheng 252000People’s Republic of China Department of Forest and Conservation sciences Faculty of ForestryUniversity of British ColumbiaVancouverBC V6T 1Z4Canada

Understanding the relationship between forest management and water use efficiency(WUE)is important for evaluating forest adaptability to climate ***,the effects of thinning and understory removal on WUE and its key controlling processes are not well understood,which limits our comprehension of the physiological mechanisms of various management *** this study,four forest management measures(no thinning:NT;understory removal:UR;light thinning:LT;and heavy thinning:HT)were carried out in Pinus massoniana plantations in a subtropical region of *** capacity and needle stable carbon isotope composition(δ^(13)C)were measured to assess instantaneous water use efficiency(WUE_(inst))and long-term water use efficiency(WUE_(i)).Multiple regression models and structural equation modelling(SEM)identified the effects of soil properties and physiological performances on WUE_(inst)and WUE_(i).The results show that WUE_(inst)values among the four treatments were ***,compared with the NT stand(35.8μmol·mol^(-1)),WUE_(i)values significantly increased to 41.7μmol·mol^(-1)in the UR,50.1μmol·mol^(-1)in the LT and 46.6μmol·mol^(-1)in HT treatments,largely explained by photosynthetic capacity and soil water *** removal did not change physiological performance(needle water potential and photosynthetic capacity).Thinning increased the net photosynthetic rate(A_n)but not stomatal conductance(g_s)or predawn needle water potential(ψ_(pd)),implying that the improvement in water use efficiency for thinned stands was largely driven by radiation interception than by soil water *** general,thinning may be an appropriate management measure to promote *** WUE to cope with seasonal droughts under future extreme climates.

关键词： Stable carbon isotope Water use efficiency Thinning Understory removal Photosynthetic capacity Needle water potential

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Influence of ion size on the charge storage mechanism of MXenes:a combination of experimental and computational

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Waste Disposal and Sustainable Energy 2024年第2期6卷 163-171页

作者： Huiwen Wan Rui wang Huiyang Fan Hongbo gao Yucheng Chen Zhu Liu State Key Laboratory of Clean Energy Utilization Institute for Thermal Power EngineeringCollege of Energy EngineeringZhejiang UniversityHangzhou 310027China china North Vehicle Research Institute No.4 HuaishulingFengtai DistrictBeijingChina ZJU-Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311215China

MXene nanomaterials have attracted great interest as the electrode of ***,its energy storage mecha-nisms in organic electrolytes are still *** work investigated the size effect of cations(i.e.,Lit,Nat,K^(+),and EMIM^(+))on the capacitive behaviors of MXene-based *** experimental results demonstrate that the specific capacitance increases obviously with decreasing cation size(i.e.,from 43 F g^(-1)(EMIM^(+))to 129 F g^(-1)(Li^(+))at 2 mV s^(-1)).Density-functional theory calculation reveals a correlation between cation size and ion-electrode surface interaction,sup-porting experimental observations of the capacitive-dominant *** dynamics simulations reveal that the ionic solvation structure and desolvation degree of intercalated cations as a function of solvation size,providing dynamic insights into the experimentally observed specific capacitance *** comprehensive experimental and computational study provides valuable insights into the intricate solvation effects governing the charge storage *** finding of ion dynamics,solvation structure,and desolvation may contribute to guide the design and optimization of appropriate ions/electrolytes combinations for MXene-based supercapacitors.

关键词： MXene Cations Molecular dynamics simulation Density-functional theory calculation Supercapacitors

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Insulin-like growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken

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science china(Life sciences) 2005年第2期48卷 187-194页

作者： wang Genyu1,YAN Bingxue1,DENG Xuemei2,LI Changlü2,HU Xiaoxiang1 & Li Ning1 1.State Key Laboratory for Agrobiotechnology,china agricultural university,beijing 100094,china 2.Department of Animal Genetics and Breeding,college of Animal science and Technology,china agricultural university,beijing 100094,china State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China Department of Animal Genetics and Breeding College of Animal Science and Technology China Agricultural University Beijing 100094 China

We have identified DNA polymorphisms in the gene of insulin-like growth factor 2 by PCR-SSCP in a resource population, which was generated by Silky reciprocally crossing to Broilers. A C→G mutation was detected in the exon 2 (at position 71) by sequencing. This single nucleotide polymorphism (SNP) was found to be associated with production traits. Chicken with BB genotype showed more chest angle width but less 3 week body weight and glandular stom- ach weight than chicken with AA genotype (P2 locus was imprinting. Progeny from heterozygote × homozygote reciprocal cross was assayed for ex- pression after the genotype was determined. The transcription of IGF2 was detected by RT-PCR-SSCP. IGF2 gene was expressed bialleleically in 1-day-old neonatal liver and 90-day-old liver, kidney, heart, and muscle of both heterozygote AB and BA chickens. Therefore, IGF2 was not an imprinting gene in chicken. The different genetic effects between the het- erozygote AB and BA remain to be elucidated.

关键词： chicken, IGF2, single nucleotide polymorphism, genome imprinting.

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Study on mapping Quantitative Trait Loci for animal complex binary traits using Bayesian-Markov chain Monte Carlo approach

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science china(Life sciences) 2006年第6期49卷 552-559页

作者： LIU Jianfeng1,2,ZHANG Yuan1,ZHANG Qin1,wang Lixian2 & ZHANG Jigang1 1. college of Animal science and Technology,china agricultural university,beijing 100094,china 2. Institute of Animal science,Chinese Academy of agricultural sciences,beijing 100094,china college of Animal science and Technology China Agricultural University Beijing 100094 China Institute of Animal science Chinese Academy of Agricultural Sciences Beijing 100094China college of Animal science and Technology China Agricultural University Beijing 100094 China Institute of Animal science Chinese Academy of Agricultural Sciences Beijing 100094 China

It is a challenging issue to map Quantitative Trait Loci (QTL) underlying complex discrete traits, which usually show discontinuous distribution; less information, using conventional statistical methods. Bayesian-Markov chain Monte Carlo (Bayesian-MCMC) approach is the key procedure in mapping QTL for complex binary traits, which provides a complete posterior distribution for QTL parameters using all prior information. As a consequence, Bayesian estimates of all interested variables can be obtained straightforwardly basing on their posterior samples simulated by the MCMC algorithm. In our study, utilities of Bayesian-MCMC are demonstrated using simulated several animal outbred full-sib families with different family structures for a complex binary trait underlied by both a QTL; polygene. Under the Identity-by-Descent-Based variance component random model, three samplers basing on MCMC, including Gibbs sampling, Metropolis algorithm; reversible jump MCMC, were implemented to generate the joint posterior distribution of all unknowns so that the QTL parameters were obtained by Bayesian statistical inferring. The results showed that Bayesian-MCMC approach could work well; robust under different family structures; QTL effects. As family size increases; the number of family decreases, the accuracy of the parameter estimates will be improved. When the true QTL has a small effect, using outbred population experiment design with large family size is the optimal mapping strategy.

关键词： complex binary trait, QTL mapping, Bayesian-MCMC approach, outbred population, IBD-based variance component random model.

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气候变化背景下农田土壤碳储量评估方法研究进展

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农业工程学报 2024年第16期40卷 1-11页

作者：王磊高阳沈振中国农业大学土地科学与技术学院北京100193 国家自然科学基金委员会地球科学部北京100085

气候变化对自然和社会生态系统产生了深远影响。农田土壤碳储量作为陆地碳库的重要组成部分,不仅影响全球气候变化,也关系到农业可持续发展和全球粮食安全。通过梳理相关文献,探讨气候变化对农田土壤碳储量的影响,探究农田土壤碳储量评... 详细信息

气候变化对自然和社会生态系统产生了深远影响。农田土壤碳储量作为陆地碳库的重要组成部分,不仅影响全球气候变化,也关系到农业可持续发展和全球粮食安全。通过梳理相关文献,探讨气候变化对农田土壤碳储量的影响,探究农田土壤碳储量评估方法的优缺点及其适用性。论文总结了气候变化单因子(气温升高、降水模式变化、二氧化碳浓度升高、大气氮沉降和各种极端天气事件)及其多因子联合(温度-降水、温度-降水-二氧化碳和二氧化碳-氮沉降-臭氧)对农田土壤碳储量的影响,分析了包括统计分析方法、基于机理的过程模拟模型方法和组合模型方法的农田土壤碳储量评估方法优缺点。最后,从关注微生物介导的土壤碳储存机制、聚焦气候变化多因子交互效应、发展综合模拟模型、模型不确定性研究4个方面提出了未来研究方向。

关键词：气候变化农田土壤碳储量模型

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Fine Mapping QTLs Affecting Milk Production Traits on BTA6 in Chinese Holstein with SNP Markers

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Journal of Integrative Agriculture 2013年第1期12卷 110-117页

作者： LIU Rui SUN Dong-xiao wang Ya-chun YU Ying ZHANG Yi CHEN Hui-yong ZHANG Qin ZHANG Sheng-li ZHANG Yuan college of Animal science and Technology/Key Laboratory of Animal Genetics and Breeding Ministry of Agriculture/National Engineering Laboratory of Animal Genetics/China Agricultural University Sichuan Animal science Academy

Our previous studies demonstrated that the region around markers BMS470 and BMS1242 on BTA6 showed a linkage to 305-d milk yield and composition traits in the Chinese Holstein population. We herein focused on such narrow region to fine map milk production QTLs with 15 SNPs across 25 Mb with each SNP in 1 Mb within most regions in a Chinese Holstein population with daughter design. 1 449 Holstein cows and 11 sires were genotyped for such SNPs by using TaqMan probe and RFLP assays. Multipoint linkage analysis across family revealed a QTL affecting milk yield between PPARGC1A C4075T and SLC34A2 T1713C. Meanwhile, within family analysis found three milk yield QTLs （two in CR T60984131G-CEP135 C501T and one in PDLIM5 A106C-OPN T3907, a fat yield QTLin UGDH T1670C-CR T60984131G region, and two protein yield QTLs in TBC1D1 G501C-UGDH T1670C and PPARGC1A C4075T-SLC34A2 T1713C, respectively. Associations between aforementioned significant SNP markers and milk production traits were further implemented. We found significant associations of PPARGC1A C4075T, SLC34A2 T1713C with milk yield （P0.05, P0.01, P0.01）, UGDH T1670C, and CR T60984131G with fat yield （P0.01, P0.01）, and PPARGC1A C4075T, SLC34A2 T1713C, UGDH T1670C and OPN T3907 with protein yield （P0.01, P0.01, P0.01, P0.01）. Our findings implied that QTLs affecting milk production traits on BTA6 were pleictropism or multigenic effect and PPARGC1A and OPN may be the causal mutations behind milk production QTLs on BTA6 in the Chinese Holstein population.

关键词： fine mapping milk production trait SNP BTA6 Chinese Holstein

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Rumen metagenome reveals the mechanism of mitigation methane emissions by unsaturated fatty acid while maintaining the performance of dairy cows

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Animal Nutrition 2024年第3期18卷 296-308页

作者： Zhantao yang Yuhui Zheng Siyuan Liu Tian Xie Qianqian wang Zhonghan wang Shengli Li Wei wang State Key Laboratory of Animal Nutrition and Feeding Beijing Engineering Technology Research Center of Raw Milk Quality and Safety ControlCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Dietary fat content can reduce the methane production of dairy cows;however,the relevance fatty acid(FA)composition has towards this inhibitory effect is ***,in-depth studies elucidating the effects of unsaturated fatty acids(UFA)on rumen function and the mechanism of reducing methane(CH4)production are *** study exposed 10 Holstein cows with the same parity,similar milk yield to two total mixed rations:low unsaturated FA(LUFA)and high unsaturated FA(HUFA)with similar fat *** LUFA group mainly added fat powder(C16:0>90%),and the HUFA group mainly replaced fat powder with extruded *** experiment lasted 26 d,the last 5 d of which,gas exchange in respiratory chambers was conducted to measure gas *** found that an increase in the UFA in diet did not affect milk production(P>0.05)and could align the profile of milk FAs more closely with modern human nutritional ***,we found that increasing the UFA content in the diet lead to a decrease in the abundance of Methanobrevibacter in the rumen(|linear discriminant analysis[LDA]score|>2 and P 2 and P<0.05),which ultimately decreased CH4 production(P<0.05).Our results illustrated the mechanism involving decreased CH4 production when fed a UFA diet in dairy *** believe that our study provides new evidence to explore CH4 emission reduction measures for dairy cows.

关键词： Methane emission Rumen metagenome Dairy cow Unsaturated fatty acid

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