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Fabrication of MXene-Bi_(2)WO_(6)heterojunction by Bi_(2)Ti_(2)O_(7)hinge for extraordinary LED-light-driven photocatalytic performance

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Nano Research 2024年第6期17卷 4729-4736页

作者： Zhiwen Li Liangliang xu Zaheer Ud Din Babar Ali Raza Yifei Zhang Xinrui Gu Yu-Xin Miao Zhen zhao gao Li Institute of Catalysis for Energy and Environment College of Chemistry and Chemical EngineeringShenyang Normal UniversityShenyang 110034China State Key Laboratory of Catalysis Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China Scuola Superiore Meridionale(SSM) University of Naples Federico IILargo S.Marcellino1080138Italy Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology(KAIST)Daejeon 34141Republic of Korea university of Chinese Academy of sciences Beijing 100049China

Heterojunction composites with intimate interfaces can shorten the diffusion distance,which leads to a shorter path for photogenerated carriers,thereby increasing photocatalytic ***,we report the fabrication of Ti_(3)C_(2)-Bi_(2)WO_(6)(TC-BW)heterojunctions hinged by Bi_(2)Ti_(2)O_(7)joints via an in situ hydrothermal reaction of Ti_(3)C_(2)in the presence of Na_(2)WO_(4)and Bi(NO_(3))_(3).The TC-BW was characterized using X-ray diffraction(XRD),scanning transmission electron microscopy(STEM),and Raman ***-BW showed superior photocatalytic activity(productivity over 15TC-WB reaches up to 5.0 mmolreacted BA·gcat.^(−1)·h^(−1))in the oxidation of benzyl alcohol using light-emitting diode(LED)light,arising from the surface defects and intimate heterojunction interface between the Ti_(3)C_(2)MXene and Bi_(2)WO_(6)***-BW heterojunctions provide an enhanced separation efficiency of photogenerated charges,which in turn yields superior photocatalytic ***,it is well substantiated by density functional theory(DFT)*** summary,this study elucidates the preparation of heterojunction composites with intimate interfaces for highly efficient photooxidation.

关键词： MXene-Bi_(2)WO_(6) heterojunction LED-light-driven photocatalysis Bi_(2)Ti_(2)O_(7) photooxidation

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Determination of 23 β_2-agonists and 5 β-blockers in animal muscle by high performance liquid chromatography-linear ion trap mass spectrometry

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science china Chemistry 2010年第4期53卷 832-840页

作者： WU YongNing1,2, MIAO Hong1, FAN Sai1,2 & zhao YunFeng1,2 1 Institute of Nutrition and Food Safety, Chinese Centre for Disease Control and Prevention, beijing 100021, china 2 college of Veterinary Medicine, china agricultural university, beijing 100193, china Institute of Nutrition and Food Safety Chinese Centre for Disease Control and Prevention Beijing China college of Veterinary Medicine China Agricultural University Beijing China

A high performance liquid chromatography-linear ion trap mass spectrometry method using isotope dilution technique has been developed for the simultaneous determination of 23 β2-agonists and 5 β-blockers in animal muscle tissues. Pork and chicken muscle samples were acid hydrolyzed and extracted with 5% trichloracetic acid in water, and then cleaned up using MCX solid phase extraction (SPE) cartridge. Methanol and 0.1% formic acid were used as mobile phases for gradient elution. A Waters AtlantisT3 column was used for separation. ESI positive ion scan mode was used with selective reaction monitoring. 9 β2-Agonists labeled by the deuterium isotope were used as internal standards for quantification. The linear ranges of 23 β2-agonists and 5 β-blockers were 5-200 μg/L, the coefficient of correlation was not less than 0.995, and the limit of detection for each compound in the muscle tissue was below 0.2 μg/kg. The recoveries of each compound in the spiked samples at three levels 5, 10, 20 μg/kg were in the range of 47.3%-123.7%, and the relative standard deviations were in the range of 3.2%-25.7%. The developed method is sensitive and specific for the determination of β2-agonists and β-blockers in pork and chicken muscle samples.

关键词： β2-agonists β-blockers muscle determination HPLC-ITMS

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An Ontology-Based Information Retrieval Model for Vegetables E-Commerce

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Journal of Integrative Agriculture 2012年第5期11卷 800-807页

作者： TAO Teng-yang zhao Ming college of Information and Electrical Engineering China Agricultural UniversityBeijing 100083P.R.China

With the rapid increment of the information on the web, traditional information retrieval based on the keywords is far from user＇s satisfaction in recall and precision. In order to improve the recall ratio and the precision radio of IR engine in the vegetables e-commerce, an information retrieval model based on the vegetables e-commerce ontology is presented in this paper, vegetables e-commerce ontology was constructed by gathering and the analyzing vegetables e-commerce domain information on the web. The vegetables e-commerce ontology is composed of some kinds of vegetable classes and hierarchy relationship of vegetables classes. In the process of information retrieval, domain ontology helps to index information and information inference. An ontology-based information retrieval model is implemented, and which has more functions than the keyword-based web information retrieval engines. The experiment results show that the recall ratio and the precision ratio of ontology-based information retrieval model are higher than that of the information retrieval engine based on keyword at a certain extent.

关键词： domain-ontology vegetables e-commerce information retrieval

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A five-fold pig bacterial artificial chromosome library:a resource for positional cloning and physical mapping

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Progress in Natural science:Materials International 2006年第8期16卷 889-892页

作者： LIU Wei, ZHANG Ying, LIU zhaoliang, GUO Li, WANG Xiaobo, FEI Jing, FENG Jidong, zhao Rui, HU Xiaoxiang and LI Ning (The State Key Laboratory for Agro-Biotechnology, college of Biology, china agricultural university, beijing 100094, china college of Animal science and Technology, china agricultural university, beijing 100094, china) The State Key Laboratory for Agro-Biotechnology College of Biology China Agricultural University Beijing 100094 China college of Animal science and Technology China Agricultural University Beijing 100094 China

A pig BAC library was constructed with genomic DNA from a male Erhualian pig. After partial digestion with HindⅢor BamHⅠthe fragments obtained were cloned into the pBeloBAC11 vector. The library consists of 184320 clones which stored in 480 pieces 384-well plates (20 plates per superpool). A two-step 4-dimension PCR screening system was established to screen the positive clones. An average insert size of 128 kb was estimated from 105 randomly isolated clones, which indicates that the library is more than five times of genomic coverage. For the demonstration of the probability to pick out any unique genes or DNA markers from the library, 10 single-copy genes were screened out and the positive clones were yielded between 1 and 8 with an average of 3.6. Positive superpools were obtained for 32 microsatellite markers selected from different regions of pig genome. The number of positive superpools for each marker varies from 1 to 9 with an average of 4.78. This BAC library provides an additional resource for pig physical mapping and gene identifica-

关键词： pig BAC library PCR screening system.

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Effect of physiological heterogeneity of E. coli population on antibiotic susceptivity test

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science china(Life sciences) 2007年第6期50卷 808-813页

作者： LIU YuQing1,3, ZHANG HuaiQiang2, SHEN JianZhong3 & gao PeiJi2 1 Institute of Animal science and Veterinary Medicine, Shandong Academy of agricultural science, Jinan 250100, china 2 State Key Laboratory of Microbial Technology, Shandong university, Jinan 250100, china 3 college of Veterinary Medicine, china agricultural university, beijing 100094, china Institute of Animal science and Veterinary Medicine Shandong Academy of Agricultural Science Jinan China college of Veterinary Medicine China Agricultural University Beijing China State Key Laboratory of Microbial Technology Shandong University Jinan China

According to the instantaneous growth rate (dN/dt) of E. coli CVCC249 growing in batch culture, the entire growth progress was distinguished into four phases: accelerating growth phase, constant growth phase, decelerating growth phase; declining phase, in each of which obvious variation in physiological; biochemical properties was detected, including total DNA, total protein,; MTT-dehydrogenase activity, etc., that led to difference in their antibiotic susceptivity. Antibiotic susceptivity of the population sampled from each phase was tested by Concentration-killing Curve (CKC) approach following the formula N=N 0/{1+exp[r·(x-BC 50)]}, showing as normal distribution at the individual cell level for an internal population, in which the median bactericidal concentration BC 50 represents the mean level of susceptivity, while the bactericidal span BC 199=(2lnN 0)/r indicates the variation degree of the antibiotic susceptivity. Furthermore, tested by CKC approach, the antibiotic susceptivity of E. coli CVCC249 population in each physiological phase to gentamicin or enoxacin was various: susceptivity of the population in the constant growth phase; declining phase all increased compared with that in the accelerating growth phase for gentamicin but declined for enoxacin. The primary investigations revealed that the physiological phase should be taken into account in the context of antibiotic susceptivity; research into antimicrobial mechanism. However there are few reports concerned with this study. Further research using different kinds of antibiotics with synchronized continuous culture of different bacterial strains is required.

关键词： instantaneous growth rate, batch culture, physiological heterogeneity, concentration-killing curve, antibiotic susceptivity test

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Plant regeneration in the new era:from molecular mechanisms to biotechnology applications

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science china(Life sciences) 2024年第7期67卷 1338-1367页

作者： Chunli Chen Yuxin Hu Momoko Ikeuchi Yuling Jiao Kalika Prasad Ying Hua Su Jun Xiao Lin xu Weibing yang Zhong zhao Wenkun Zhou Yun Zhou Jian gao Jia-Wei Wang National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China college of Life science and Technology Huazhong Agricultural UniversityWuhan 430070China Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of Sciences(CAS)China National Botanical GardenBeijing 100093China Division of Biological sciences Graduate School of Science and TechnologyNara Institute of Science and TechnologyNara 630-0192Japan State Key Laboratory of Protein and Plant Gene Research School of Life SciencesPeking UniversityBeijing 100871China Peking-Tsinghua Center for Life sciences Center for Quantitative BiologyAcademy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China Indian Institute of science Education and Research Pune 411008IndiaThiruvananthapuram 695551India State Key Laboratory of Wheat Improvement College of Life SciencesShandong Agricultural UniversityTai’an 271018China Sino-German Joint Research Center on agricultural Biology Shandong Agricultural UniversityTai’an 271018China Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology(IGDB)CASBeijing 100101China CAS-JIC Centre of Excellence for Plant and Microbial science(CEPAMS) IGDBCASBeijing 100101China National Key Laboratory of Plant Molecular Genetics CEMPSInstitute of Plant Physiology and Ecology(SIPPE)CASShanghai 200032China CEPAMS SIPPECASShanghai 200032China Hefei National Laboratory for Physical sciences at the Microscale CEMPSSchool of Life SciencesDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China State Key Laboratory of Plant Environmental Resilience College of Biological SciencesChina Agricultural UniversityBeijing 100193China Department of Botany and Plant Pathology and Center for Plant Biology Purdue UniversityWest Lafayette 47907USA School of Life science and Technology Shang

Plants or tissues can be regenerated through various *** animal regeneration,cell totipotency and pluripotency are the molecular basis of plant *** systematic studies on Arabidopsis thaliana gradually unravel the fundamental mechanisms and principles underlying plant ***,plant hormones,cell division,epigenetic remodeling,and transcription factors play crucial roles in reprogramming somatic cells and reestablishing meristematic *** research on basal non-vascular plants and monocot crops has revealed that plant regeneration differs among species,with various plant species using distinct mechanisms and displaying significant differences in regenerative *** multi-omics studies at the single-cell level,tracking plant regeneration processes in real-time,and deciphering the natural variation in regenerative capacity will ultimately help understand the essence of plant regeneration,improve crop regeneration efficiency,and contribute to future crop design.

关键词： apical meristem model species regeneration phytohormone plant cell reprogramming somatic embryogenesis

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Discrete Element Modelling of Damage Evolution of Concrete Considering Meso-Structure of ITZ

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Computer Modeling in Engineering & sciences 2024年第6期139卷 3495-3511页

作者： Weiliang gao Shixu Jia Tingting zhao Zhiyong Wang Institute of Defense Engineering Academy of Military SciencesChinese People’s Liberation ArmyBeijing100850China college of Mechanical and Vehicle Engineering Taiyuan University of TechnologyTaiyuan030024China State Key Laboratory of Explosion science and Technology Beijing Institute of TechnologyBeijing100081China

The mechanical properties of interfacial transition zones(ITZs)have traditionally been simplified by reducing the stiffness of cement in previous simulation methods.A novel approach based on the discrete element method(DEM)has been developed for modeling *** new approach efficiently simulates the meso-structure of ITZs,accurately capturing their heterogeneous *** against established uniaxial compression experiments confirms the precision of *** proposedmodel canmodel the process of damage evolution containing cracks initiation,propagation and *** increasing loads,cracks within ITZs progressively accumulate,culminating in macroscopic fractures that traverse themortarmatrix,forming the complex,serpentine path of *** study reveals four distinct displacement patterns:tensile compliant,tensile opposite,mixed tensile-shear,and shear opposite patterns,each indicative of different stages in concrete’s damage *** widening angle of these patterns delineates the progression of cracks,with the tensile compliant pattern signaling the initial crack appearance and the shear opposite pattern indicating the concrete model’s ultimate failure.

关键词： Discrete element method damage evolution interfacial transition zone meso-structure model

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Genetic polymorphisms of milk epithelial mucin of yaks

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Progress in Natural science:Materials International 2006年第11期16卷 1222-1226页

作者： ZHENG Yucai~1, zhao Xingbo~2**, JIN Suyu~1, PENG Xianwen~1 and BAI Wenlin~1 (1. college of Life science and Technology, Southwest university for Nationalities, Chengdu 610041, china 2. State Key Laboratory for Agrobiotechnology and Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, china agricultural university, beijing 100094, china) college of Life science and Technology Southwest University for Nationalities Chengdu 610041 China State Key Laboratory for Agrobiotechnology Ministry of Agriculture Key Laboratory of Animal Genetics Breeding and Reproduction China Agricultural University Beijing 100094 China

Milk epithelial mucin (MUC1) of yaks was separated by SDS-polyacrylamide gel electrophoresis. A total of nine types of MUC1 were revealed in 427 yaks from five yak breeds including Maiwa yak, Jiulong yak, Tianzhu white yak, Qinghai yak and Tibetan yak. The molecular weights of MUC1 are from 163 kD to 208 kD, most of which are larger than those of bovine. Population genetic analysis shows that the gene frequency and genotype frequency of yak MUC1 differ among breeds, with relatively high gene heterozygosity. The five yak breeds studied fall into two groups according to their milk MUC1 gene frequency, suggesting that milk MUC1 exhibits specificities for local yak groups.

关键词： yak milk genetic polymorphism MUC1.

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Structural changes in the conversion of an Arabidopsis outward-rectifying K+channel into an inward-rectifying channel

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Plant Communications 2024年第6期5卷 5-8页

作者： xudong gao Xia xu Tengfei Sun Yuhan Lu Yutian Jia Jiaqi Zhou Peng Fu Yanming Zhang Guanghui yang State Key Laboratory of Plant Environmental Resilience Frontiers Science Center for Molecular Design BreedingDepartment of Nutrition and HealthCollege of Biological SciencesChina Agricultural UniversityBeijing 100193China

Dear Editor,By using cryo-electron microscopy and electrophysiology,this study characterizes the structural and functional dynamics of the K+outward-rectifying channel SKOR in Arabidopsis and the structural changes associated with a double mutation that transforms SKOR into an inward-rectifying K+channel,*** findings have implications for understanding and manipulating crop K+nutrition.

关键词： channel Arabidopsis changes

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Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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science china(Life sciences) 2008年第7期51卷 604-613页

作者： xu YueYu 1,2,3,ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3 &zhao MaoLin 1 1 beijing Agro-Biotechnology Research Center,beijing Academy of Agriculture and Forestry science,beijing 100097,china 2 Department of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,china 3 college of Life science,Capital Normal university,beijing 100037,china 4 college of Life science,Zhongkai university of Agriculture and Technology,Guangzhou 510225,china 5 college of Grassland science,Gansu agricultural university,Lanzhou 730070,china 6 Department of Biology,Henan Institute of science and Technology,Xinxiang 453003,china beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing China Department of Chemistry and Chemical Engineering Hunan Institute of Engineering Xiangtan China college of Life science Capital Normal University Beijing China college of Life science Zhongkai University of Agriculture and Technology Guangzhou China college of Grassland science Gansu Agricultural University Lanzhou China Department of Biology Henan Institute of Science and Technology Xinxiang China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 236000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 384-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

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