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The protein phosphatase qGL3/OsPPKL1 selfregulates its degradation to orchestrate brassinosteroid signaling in rice

Plant Communications 2024年第6期5卷 89-99页

作者： Xiuying gao Jianbo Li Jing Yin Yiheng zhao Zhongsheng Wu Lijuan Ma Baoyi Zhang Hongsheng Zhang Ji Huang State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization College of AgricultureNanjing Agricultural UniversityNanjing 210095China Jiangsu Provincial Engineering Research Center of Seed Industry science and Technology Nanjing 210095China

Brassinosteroids(BRs)are a class of phytohormones that regulate plant growth and *** previous studies,we cloned and identified PROTEIN PHOSPHATASE WITH KELCH-LIKE1(OsPPKL1)as the causal gene for the quantitative trait locus GRAIN LENGTH3(qGL3)in rice(Oryza sativa).We also showed that qGL3/OsPPKL1 is mainly located in the cytoplasm and nucleus and negatively regulates BR signaling and grain *** qGL3 is a negative regulator of BR signaling,its turnover is critical for rapid response to changes in ***,we demonstrate that qGL3 interacts with the WD40-domain-containing protein WD40-REPEAT PROTEIN48(OsWDR48),which contains a nuclear export signal(NES).The NES signal is crucial for the cytosolic localization of OsWDR48 and also functions in the self-turnover of *** show that OsWDR48 physically interacts with and genetically acts through qGL3 to modulate BR ***,qGL3 may indirectly promote the phosphorylation of OsWDR48 at the Ser-379 and Ser-386 *** of both phosphorylation sites in OsWDR48 to non-phosphorylatable alanine enhanced the strength of the OsWDR48-qGL3 ***,we found that brassinolide can promote the accumulation of non-phosphorylated OsWDR48,leading to strong interaction intensity between qGL3 and *** together,our results show that OsWDR48 facilitates qGL3 retention and induces degradation of qGL3 in the *** findings suggest that qGL3 self-modulates its turnover by binding to OsWDR48 to regulate its cytoplasmic localization and stability,leading to efficient orchestration of BR signal transduction in rice.

关键词： Oryza sativa brassinosteroid grain length qGL3/OsPPKL1 self-regulation

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Multiomics analysis reveals metabolic subtypes and identifies diacylglycerol kinase α (DGKA) as a potential therapeutic target for intrahepatic cholangiocarcinoma

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Cancer Communications 2024年第2期44卷 226-250页

作者： Weiren Liu Huqiang Wang Qianfu zhao Chenyang Tao Weifeng Qu Yushan Hou Run Huang Zimei Sun Guiqi Zhu Xifei Jiang Yuan Fang Jun {2. Xiaoling Wu Zhixiang yang Rongyu Ping Jiafeng Chen Rui yang Tianhao Chu Jian Zhou Jia Fan Zheng Tang Dong yang Yinghong Shi Department of Liver Surgery and Transplantation Liver Cancer InstituteZhongshan HospitalFudan UniversityKey Laboratory of Carcinogenesis and Cancer Invasion of Ministry of EducationShanghaiP.R.China Research Unit of Liver cancer Recurrence and Metastasis Chinese Academy of Medical SciencesBeijingP.R.China State Key Laboratory of Medical Proteomics Beijing Proteome Research CenterNational Center for Protein Sciences(Beijing)Beijing Institute of LifeomicsBeijingP.R.China

Background:Intrahepatic cholangiocarcinoma(iCCA)is a highly heteroge-neous and lethal hepatobiliary tumor with few therapeutic *** metabolic reprogramming of tumor cells plays an essential role in the develop-ment of tumors,while the metabolic molecular classification of iCCA is largely ***,we performed an integrated multiomics analysis and metabolic classification to depict differences in metabolic characteristics of iCCA patients,hoping to provide a novel perspective to understand and treat ***:We performed integrated multiomics analysis in 116 iCCA samples,including whole-exome sequencing,bulk RNA-sequencing and proteome *** on the non-negative matrix factorization method and the protein abundance of metabolic genes in human genome-scale metabolic models,the metabolic subtype of iCCA was *** and prognostic gene analy-ses were used to compare overall survival(OS)differences between metabolic *** proliferation analysis,5-ethynyl-2.-deoxyuridine(EdU)assay,colony formation assay,RNA-sequencing and Western blotting were performed to investigate the molecular mechanisms of diacylglycerol kinaseα(DGKA)in iCCA ***:Three metabolic subtypes(S1-S3)with subtype-specific biomarkers of iCCA were *** metabolic subtypes presented with distinct prog-noses,metabolic features,immune microenvironments,and genetic *** S2.subtype with the worst survival showed the activation of some special metabolic processes,immune-suppressed microenvironment and Kirsten ratsar-coma viral oncogene homolog(KRAS)/AT-rich interactive domain 1A(ARID1A)*** the S2.subtype-specific upregulated proteins,DGKA was further identified as a potential drug target for iCCA,which promoted cell proliferation by enhancing phosphatidic acid(PA)metabolism and activating mitogen-activated protein kinase(MAPK)***:Viamultiomics analyses,we identified three metabolic subtypes of iCCA,revealing

关键词： diacylglycerol kinaseα intrahepatic cholangiocarcinoma MAPK signaling metabolic classifi-cation multiomics analysis phosphatidic acid metabolism

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AtHsfA2.modulates expression of stress responsive genes and enhances tolerance to heat and oxidative stress in Arabidopsis

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science China(Life sciences) 2005年第6期48卷 540-550页

作者： LI Chunguang1,2. CHEN Qijun1, gao Xinqi1,3, QI Bishu1, CHEN Naizhi1, xu Shouming1, CHEN Jia1 & WANG xuechen1 1. State Key Laboratory of Plant Physiology and Biochemistry, college of Biological sciences, China {2. university, beijing 100094, China 2. Department of Industry Engineering, Zhengzhou Institute of Aeronautic Industry Management, Zhengzhou 450015, China 3. college of Life science, Qufu Normal university, Qufu Shandong 2.3165, China 1. State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University 100094 Beijing China 2. Department of Industry Engineering Zhengzhou Institute of Aeronautic Industry Management 450015 Zhengzhou China 3. college of Life science Qufu Normal University 273165 Qufu Shandong China

There is increasing evidence for considerable interlinking between the responses to heat stress and oxidative stress, and recent researches suggest heat shock transcription factors (Hsfs) play an important role in linking heat shock with oxidative stress signals. In this paper, we present evidence that AtHsfA2.modulated expression of stress responsive genes and enhanced tolerance to heat and oxidative stress in Arabidopsis. Using Northern blot and quantitative RT-PCR analysis, we demonstrated that the expression of AtHsfA2.was induced by not only HS but also oxidative stress. By functional analysis of AtHsfA2.knockout mutants and AtHsfA2.overexpressing transgenic plants, we also demonstrated that the mutants displayed reduced the basal and acquired thermotolerance as well as oxidative stress tolerance but the overexpression lines displayed increased tolerance to these stress. The phenotypes correlated with the expres-sion of some Hsps and APX1, ion leakage, H2.2.level and degree of oxidative injuries. These results showed that, by modulated expression of stress responsive genes, AtHsfA2.enhanced tolerance to heat and oxidative stress in Arabidopsis. So we suggest that AtHsfA2.plays an im-portant role in linking heat shock with oxidative stress signals.

关键词： heat shock transcription factor, heat stress response, oxidative stress response, tolerance.

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Sequences and polymorphisms of exons 3 and 4 in porcine UCP2.gene

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Progress in Natural science:Materials International 2002年第7期 73-74页

作者： FANG Meiying, LIU Chousheng, zhao Xingbo, LI Ning and WU Changxin(1. college of Animal {2. and Technology, China agricultural university, beijing 100094, China 2. National Laboratory for A-gribiotechnology, China agricultural university, beijing 100094, China 3. Gansu Institute of Animal and Veterinary {2., Pingliang 744000, China) college of Animal science and Technology，China {2. university，beijing 100094，China National Laboratory for Agribiotechnology，China agricultural university，{2. 100094，China Gansu Institute of Animal and Veterinary science，Pingliang 744000，China National Laboratory for Agribiotechnology，China agricultural university，{2. 100094，China

Uncoupling proteins are mitochondrial membrane transporters, which regulate metabolic pathways of energy balance, and are associated with biological traits of animal body weight, resting metabolic rates and energy conversion. In this study, a region of the exons 3 and 4 of pig UCP2.gene was cloned and analyzed, and a new single nucleotide polymorphic site was detected by PCR-SSCP in five pig breeds. This newfound polymorphism results from a T to G stbstitution at the position of nucleotide 2.2. which is located in intron3.

关键词： pig uncoupling proteins PCR-SSCP.

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Development of a single transcript CRISPR/Cas9 toolkit for efficient genome editing in autotetraploid alfalfa

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The Crop Journal 2024年第3期12卷 788-795页

作者： Haixia zhao Siyi zhao Yingping Cao Xiping Jiang Lijuan zhao Zhimeng Li Mengqi Wang Ruijuan yang Chuanen Zhou zhaoming Wang Feng Yuan Dongmei Ma Hao Lin Wenwen Liu Chunxiang Fu CAS Key Laboratory of Biofuels Shandong Provincial Key Laboratory of Energy GeneticsQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101ShandongChina Shandong Energy Institute Qingdao 266101ShandongChina Qingdao New Energy Shandong Laboratory Qingdao 266101ShandongChina university of Chinese Academy of sciences Beijing 100049China The Key Laboratory of Plant Development and Environmental Adaptation Biology Ministry of EducationSchool of Life SciencesShandong UniversityQingdao 266101ShandongChina Key Laboratory of Forage Breeding and Seed Production of Inner Mongolia Inner Mongolia M-Grass Ecology and Environment(Group)Co.Ltd.Hohhot 010016Inner MongoliaChina School of Ecology Environment Ningxia UniversityYinchuan 750021NingxiaChina Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China

Alfalfa(Medicago sativa.L.)is a globally significant autotetraploid legume forage ***,despite its importance,establishing efficient gene editing systems for cultivated alfalfa remains a formidable *** this study,we pioneered the development of a highly effective ultrasonic-assisted leaf disc transformation system for Gongnong 1 alfalfa,a variety widely cultivated in Northeast ***,we created a single transcript CRISPR/Cas9(CRISPR_2.0)toolkit,incorporating multiplex gRNAs,designed for gene editing in Gongnong *** Cas9 and gRNA scaffolds were under the control of the Arabidopsis ubiquitin-10 promoter,a widely employed polymeraseⅡconstitutive promoter known for strong transgene expression in *** assess the toolkit’s efficiency,we targeted PALM1,a gene associated with a recognizable multifoliate *** the CRISPR_2.0 toolkit,we directed PALM1 editing at two sites in the wild-type Gongnong *** indicated a 35.1%occurrence of editing events all in target 2.alleles,while no mutations were detected at target 1 in the transgenic-positive *** explore more efficient sgRNAs,we developed a rapid,reliable screening system based on Agrobacterium rhizogenes-mediated hairy root transformation,incorporating the visible reporter *** screening system demonstrated that most purple visible hairy roots underwent gene ***,sgRNA3,with an 83.0%editing efficiency,was selected using the visible hairy root *** anticipated,tetra-allelic homozygous palm1 mutations exhibited a clear multifoliate *** palm1 lines demonstrated an average crude protein yield increase of 2..5%compared to trifoliolate *** findings highlight the modified CRISPR_2.0 system as a highly efficient and robust gene editing tool for autotetraploid alfalfa.

关键词： Alfalfa Gene editing CRISPR_2.0 toolkit Hairy root system Tetra-allelic homozygous mutants

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Peroxidase Isoenzyme and RAPD Analysis on the Mutagenic Strains of Inonotus obliquus

Peroxidase Isoenzyme and RAPD Analysis on the Mutagenic Stra...

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第五届国际药用菌大会

作者： LIJIE ZHOU~1,LI zhao~1,BAIHONG QU~1,YANQIU CHEN~*,YU LI~2.& BAOKAI CUI~3 1 agricultural {2. of Yanbian university,Longjing 133400,Jilin,China 2.Institute of Mycology Jilin agricultural university,Changchun 130118,China 3 Institute of Microbiology,beijing Forestry university,beijing 100083,China

Peroxide isoenzyme pattern and RAPD profiling of Inonotus obliquus strain JL01 and other three strains JLZ1、JLZ2.JLC 1 which were mutated from JL01 by ultraviolet radiation and C were conducted in order to determine genetic *** number and colour of isozyme bands and DNA fragments of JLZ1、JLZ2.JLC 1 were different from JL01,indicating that genes of mutagenic strains relative to the original one had changed greatly.

关键词： Ultraviolet radiation 60Co mutagenic strains genetic divergence

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Assessing the conservation impact of Chinese indigenous chicken populations between ex-situ and in-situ using genome-wide SNPs

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Journal of Integrative Agriculture 2024年第3期23卷 975-987页

作者： Wenting Li Chaoqun gao zhao Cai Sensen Yan Yanru Lei Mengya Wei Guirong Sun Yadong Tian Kejun Wang Xiangtao Kang college of Animal science and Technology Henan Agricultural UniversityZhengzhou 450002China The Shennong Laboratory Zhengzhou 450002China

Conservation programs require rigorous evaluation to ensure the preservation of genetic diversity and viability of conservation populations. In this study, we conducted a comparative analysis of two indigenous Chinese chicken breeds, Gushi and Xichuan black-bone, using whole-genome SNPs to understand their genetic diversity, track changes over time and population structure. The breeds were divided into five conservation populations(GS1, 2.10, ex-situ;GS2. 2.19, ex-situ;GS3, 2.19, in-situ;XB1, 2.10, in-situ;and XB2. 2.19, in-situ) based on conservation methods and generations. The genetic diversity indices of three conservation populations of Gushi chicken showed consistent trends, with the GS3 population under in-situ strategy having the highest diversity and GS2.under ex-situ strategy having the lowest. The degree of inbreeding of GS2.was higher than that of GS1 and GS3. Conserved populations of Xichuan black-bone chicken showed no obvious changes in genetic diversity between XB1 and XB2. In terms of population structure, the GS3 population were stratified relative to GS1 and GS2. According to the conservation priority, GS3 had the highest contribution to the total gene and allelic diversity in GS breed, whereas the contribution of XB1 and XB2.were similar. We also observed that the genetic diversity of GS2.was lower than GS3, which were from the same generation but under different conservation programs(in-situ and ex-situ). While XB1 and XB2.had similar levels of genetic diversity. Overall, our findings suggested that the conservation programs performed in ex-situ could slow down the occurrence of inbreeding events, but could not entirely prevent the loss of genetic diversity when the conserved population size was small, while in-situ conservation populations with large population size could maintain a relative high level of genetic diversity.

关键词： genome-wide SNPs conservation genetic diversity ex-situ in-situ

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The complex roles of m^(6)A modifications in neural stem cell proliferation, differentiation, and self-renewal and implications for memory and neurodegenerative diseases

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Neural Regeneration Research 2025年第6期20卷 1582-1598页

作者： Yanxi Li Jing xue Yuejia Ma Ke Ye xue {2. Fangliang Ge Feifei Zheng Lulu Liu xu gao Dayong Wang Qing Xia Department of Biochemistry and Molecular Biology College of Basic Medical SciencesHarbin Medical UniversityHarbinHeilongjiang ProvinceChina college of Basic Medical sciences Harbin Medical UniversityHarbinHeilongjiang ProvinceChina Basic Medical Institute Heilongjiang Academy of Medical SciencesHarbinHeilongjiang ProvinceChina Key Laboratory of Heilongjiang Province for Genetically Modified Animals Harbin Medical UniversityHarbinHeilongjiang ProvinceChina Translational Medicine Research and Cooperation Center of Northern China Heilongjiang Academy of Medical SciencesHarbinHeilongjiang ProvinceChina Dongzhimen Hospital Beijing University of Chinese MedicineBeijingChina

N6-methyladenosine(m^(6)A), the most prevalent and conserved RNA modification in eukaryotic cells, profoundly influences virtually all aspects of mRNA metabolism. mRNA plays crucial roles in neural stem cell genesis and neural regeneration, where it is highly concentrated and actively involved in these processes. Changes in m^(6)A modification levels and the expression levels of related enzymatic proteins can lead to neurological dysfunction and contribute to the development of neurological diseases. Furthermore, the proliferation and differentiation of neural stem cells, as well as nerve regeneration, are intimately linked to memory function and neurodegenerative diseases. This paper presents a comprehensive review of the roles of m^(6)A in neural stem cell proliferation, differentiation, and self-renewal, as well as its implications in memory and neurodegenerative diseases. m^(6)A has demonstrated divergent effects on the proliferation and differentiation of neural stem cells. These observed contradictions may arise from the time-specific nature of m^(6)A and its differential impact on neural stem cells across various stages of development. Similarly, the diverse effects of m^(6)A on distinct types of memory could be attributed to the involvement of specific brain regions in memory formation and recall. Inconsistencies in m^(6)A levels across different models of neurodegenerative disease, particularly Alzheimer's disease and Parkinson's disease, suggest that these disparities are linked to variations in the affected brain regions. Notably, the opposing changes in m^(6)A levels observed in Parkinson's disease models exposed to manganese compared to normal Parkinson's disease models further underscore the complexity of m^(6)A's role in neurodegenerative processes. The roles of m^(6)A in neural stem cell proliferation, differentiation, and self-renewal, and its implications in memory and neurodegenerative diseases, appear contradictory. These inconsistencies may be a

关键词： Alzheimer's disease cell self-renewal central nervous system memory microglia nerve regeneration neurodegenerative diseases neurogenesis RNA methylation

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Asymmetric side-chain engineering of organic semiconductor for ultrasensitive gas sensing

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Chinese Chemical Letters 2024年第3期35卷 428-433页

作者： Xiaoying Ma Xiaojuan Dai Lanyi Xiang Jiajun Chang Danfeng Zhi Haozhen zhao Zhenjie Ni Ye Zou Xike gao Fengjiao Zhang School of Chemical sciences University of Chinese Academy of SciencesBeijing 100049China Laboratory of Organic Solids Institute of ChemistryChinese Academy of SciencesBeijing 100190China Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China Binzhou Institute of Technology Binzhou 256606China

Molecular structure of organic semiconductor plays a critical role in determining the performance and functionality of organic electronic devices,by optimizing the electrical,optical and physicochemical *** alkyl chains are fundamental units in tailering the solubility and assemblability,among which the asymmetric properties have been reported as key element for controlling the packing motifs and intrinsic charge ***,we expanded the scope of molecular asymmetry dependent sensing features based on a new series of naphthalene diimides(NDI)-based derivatives substituted with a same branching alkyl chain but various linear-shaped alkyl chains(Cn-).A clear molecular stacking change,from head-to-head bilayer to head-to-tail monolayer packing model,is observed based on the features of anisotropic molecular interactions with the change in the chain *** importantly,a unique LUMO level shift of 0.17 eV is validated for NDI-PhC4,providing a record sensitivity up to 150%to 0.01 ppb ammonia,due to the desired molecular reactivity and device amplification *** results indicate that asymmetric side-chain engineering opens a route for breath healthcare.

关键词： Organic thin film transistor Asymmetric side chain Gas sensing Molecular stacking Energy level manipulating

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Optimization of inter-seasonal nitrogen allocation increases yield and resource-use efficiency in a water-limited wheat-maize cropping system in the North China Plain

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The Crop Journal 2024年第3期12卷 907-914页

作者： Xiaonan Zhou Chenghang Du Haoran Li Zhencai Sun Yifei Chen Zhiqiang gao Zhigan zhao Yinghua Zhang Zhimin Wang Ying Liu college of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China college of Information and Electrical Engineering China Agricultural UniversityBeijing 100193China Ministerial and Provincial Co-Innovation Centre for Endemic Crops Production with High-Quality and Efficiency in Loess Plateau Taigu 030801ShanxiChina

Winter wheat–summer maize cropping system in the North China Plain often experiences droughtinduced yield reduction in the wheat season and rainwater and nitrogen(N)fertilizer losses in the maize *** study aimed to identify an optimal interseasonal water-and N-management strategy to alleviate these *** ratios of allocation of 360 kg N ha^(-1)between the wheat and maize seasons under one-time presowing root-zone irrigation(W0)and additional jointing and anthesis irrigation(W2.in wheat and one irrigation after maize sowing were set as follows:N1(12.:2.0),N2.180:180),N3(2.0:12.)and N4(300:60).The results showed that under W0,the N3 treatment produced the highest annual yield,crop water productivity(WPC),and nitrogen partial factor productivity(PFPN).Increased N allocation in wheat under W0 improved wheat yield without affecting maize yield,as surplus nitrate after wheat harvest was retained in the topsoil layers and available for the subsequent *** W2.annual yield was largest in the N2.*** risk of nitrate leaching increased in W2.when N application rate in wheat exceeded that of the N2.treatment,especially in the wet *** to W2.2.the W0N3 maintained 95.2.grain yield over two *** WPCwas higher in the W0 treatment than in the W2.***,following limited total N rate,an appropriate fertilizer N transfer from maize to wheat season had the potential of a“triple win”for high annual yield,WPCand PFPN in a water-limited wheat–maize cropping system.

关键词： Cropping system Water-saving irrigation North China Plain Nitrogen optimization Sustainable intensification

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