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Analysis of tandem repeats in the genome of chinese shrimp Fenneropenaeus chinensis

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chinese Science Bulletin 2005年第14期50卷 1462-1469页

作者： KONG Jie1 & gao Huan2,3.1. Key Laboratory for Sustainable Utilization of Marine Fisheries Re- sources, Ministry of Agriculture, Yellow Sea Fisheris Research Insti- tute, chinese academy of Fishery {3., Qingdao 266071,{3. 2. Institute of oceanology, chinese academy of sciences, Qingdao 266071,{3. 3. The Graduate School of the chinese academy of sciences, Beijing 10003.,{3. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of Agriculture Yellow Sea Fisheris Research Institute Chinese Academy of Fishery Sciences Qingdao China Institute of oceanology Chinese Academy of Sciences Qingdao China The Graduate School of the chinese academy of sciences Beijing China

Through random sequencing, we found a total of 884000 base-pairs (bp) of random genomic sequences in the genome of chinese shrimp (Fenneropenaeus chinensis). Using bio-soft Tandem Repeat Finder (TRF) software, 2159 tandem repeats were found, in which there were 1714 mi- crosatellites and 445 minisatellites, accounting for 79.4% and 20.6% of repeat sequences, respectively. The cumulative length of repeat sequences was found to be 116685 bp, ac- counting for 13.2% of the total DNA sequence; the cumula- tive length of microsatellites occupied 9.78% of the total DNA sequence, and that of minisatellites occupied 3.42%. In decreasing order, the 20 most abundant repeat sequence classes were as follows: AT (557), AC (471), AG (274), AAT (92), A (56), AAG (28), ATC (27), ATAG (27), AGG (18), ACT (15), C (11), AAC (11), ACAT (11), CAGA (10), AGAA (9), AGGG (7), CAAA (7), CGCA (6), ATAA (6), AGAGAA (6). Dinucleotide repeats, not only in the aspect of the number, but also in cumulative length, were the preponderant repeat type. There were few classes and low copy numbers of repeat units of the pentanucleotide repeat type, which included only three classes: AGAGA, GAGGC and AAAGA. The classes and copy numbers of heptanucleotide, eleven-nucleotide and thirteen-nucleotide primer-number-composed repeats were distinctly less than that of repeat types beside them.

关键词：虾遗传特性遗传基因 DNA

Isolation and Characterization of Photosystem Ⅱ of Porphyra yezoensis Ueda

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 780-785页

作者： Zheng-Quan gao, Guang-Ce WANG, and Cheng-Kui TSENGKey Laboratory of Experimental Marine Biology, Institute of oceanology, the chinese academy of sciences, Qingdao 266071,{3. Graduate school, the chinese academy of sciences, Beijing 10003.,{3. Key Laboratory of Experimental Marine Biology Institute of Oceanology the Chinese Academy of SciencesQingdao 266071 China Graduate school the Chinese Academy of SciencesBeijing 100039 China

The thylakoid membranes were isolated and purified from gametophyte of Porphyra yezoensis Ueda (P. yezoensis) by sucrose density gradient ultracentrifugation. After P. yezoensis gametophyte thylakoid membranes were so... 详细信息

关键词： red algae Porphyra yezoensis Ueda photosystem Ⅱ polypeptide composition extrinsic protein

Direct Optimization of Low-thrust Many-revolution Earth-orbit Transfers

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chinese Journal of Aeronautics 2009年第4期22卷 426-433页

作者： gao Yang academy of Opto-Electronics,chinese academy of {3.,Beijing 100190,china [a]academy of Opto-Electronics Chinese Academy of Sciences Beijing 100190 China

Low-thrust Earth-orbit transfers with 10^- 5-order thrust-to-weight ratios involve a large number of orbital revolutions which poses a real challenge to trajectory optimization. This article develops a direct method to optimize minimum-time low-thrust many-revolution Earth-orbit transfers. A parameterized control law in each orbit, in the form of the true optimal control, is proposed, and the time history of the parameters governing the control law is interpolated through a finite number of nodal values. The orbital averaging method is used to significantly reduce the computational workload and the trajectory optimization is conducted based on the orbital averaging dynamics expressed by nonsingular equinoctial elements. Furthermore, Earth＇s shadowing and perturbation effects are taken into account. The optimal transfer problem is thus converted to the parameter optimization problem that can be solved by nonlinear programming. Taking advantage of the mapping between the parameterized control law and the Lyapunov control law, a technique is proposed to acquire good initial guesses for optimization variables, which results in enlarged convergence domain of the direct optimization method. Numerical examples of optimal Earth-orbit transfers are presented.

关键词： low thrust orbital transfer optimization direct method orbital averaging Lyapunov method

Two possible mechanisms for vortex self-organization

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Science china Earth sciences 2006年第2期49卷 202-211页

作者： LUO Zhexian2, ZHOU Xiuji1 & gao Shouting3.1. chinese academy of Meteorological {3., Beijing 100081,{3. 2. Department of Space Information Science, Nanjing University of Information Science and Technology, Nanjing 210044,{3. 3. Institute of Atmospheric Physics, chinese academy of sciences, Beijing 100029,{3. 2. Department of Environmental Science Nanjing University of Information Science and Technology Nanjing 210044 China 1. chinese academy of Meteorological sciences Beijing 100081 China 3. Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China

The vortex self-organization is investigated in this paper by four groups of numerical experiments within the framework of quasi-geostrophic model, and based on the experimental results two types of possible mechanisms for vortex self-organization are suggested. The meso-scale to-pography may enable separated vortices to merge into a larger scale vortex; and the interaction of meso-γ and meso-β scale systems may make separated vortices to self organize a typhoon-like vor-tex circulation.

关键词： vortex, self-organization, meso-scale topography, interaction of different scales.

Sequence analysis of mRNA polyadenylation signals of rice genes

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chinese Science Bulletin 2006年第9期51卷 1069-1077页

作者： LU Ying1, 2, gao Chenxi1,2,3.& HAN Bin1,3.41. National Center for Gene Research, Shanghai Institutes for Biological sciences, chinese academy of sciences, Shanghai 20023.,{3. 2. Graduate School of the chinese academy of sciences, Beijing 10003.,{3. 3. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological sciences, chinese academy of sciences, Shanghai 20003.,{3. National Center for Gene Research Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200233 China Graduate School of the chinese academy of sciences Beijing 100039 China Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

The formation of eukaryotic mRNAs involves the cleavage and polyadenylation of pre- mRNAs. To investigate the sequence requirement of putative polyadenylation signals (PASs), poly(A) sites and downstream elements (DUEs) in 3.-end-pro- cessing in rice, we compared expressed sequences tags (ESTs) with poly(A) extremity to full-length cDNA sequences and constructed a database of 12969 pre- mRNA sequences in ?40―+40 nt surrounding the poly(A) sites, which were from 9953.genes. The al- ternative poly(A) sites were revealed in approxi- mately 25% of mRNAs. Nearly 80% of pre-mRNAs showed stringent requirement of the YA (CA or UA) at poly (A) sites for polyadenylation. About 7.9% had the AAUAAA signals on ?40―?1 nt upstream of the poly(A) sites. Over 60% of mRNAs probably used the one- or two-base variants of AAUAAA hexamers as their PASs in 3. fragments. The single-base variants of AAUGAA revealed the high frequency in 11.5% of 3. fragments. The DUEs were detected in 90% of pre- mRNAs, especially more than half of the pre-mRNAs with multi-base variants of AAUAAA had the DUEs surrounding the poly(A) site. The location of DUE is also important for defining the cleavage site. Al- though most of the rice pre-mRNAs did not contain AAUAAA signal, the existence of downstream ele- ments ensured the efficiency of cleavage-polyade-

关键词：多聚腺苷酸序列分析 mRNA 水稻基因工程

Dissolved inorganic carbon and CO_(2) fluxes across Jiaozhou Bay air-water interface

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Acta Oceanologica Sinica 2004年第2期23卷 279-285页

作者： LIXuegang LINing gaoXuelu SONGJinming Instituteofoceanology ChineseAcademyofSciencesQingdao266071China GraduateSchool ChineseAcademyofSciencesBeijing100039China

On the basis of data collected in the Jiaozhou Bay in June and July 2003. the DIC distribution in seawater is studied, and an average air-sea flux of CO2 is estimated. The results show that the content of DIC inside the bay is markedly higher than outside the bay in June, but the content of DIC outside the bay is markedly higher than inside the bay in July. The trend of DIC distribution inside the bay is similar, viz. the content is the maximum in the northeast, then decreases gradually toward the west, and the content is the minimum in the west. The total trend of vertical distribution is to increase gradually from surface to bottom. This characteristic of DIC distribution is determined by Jiaozhou Bay hydrology and there is a close relation between DIC and particulate N,P. Average CO2flux across the air-sea interface is 0.55 mol/(m2-a) in June and 0.72 mol/(m2-a) in July. Jiaozhou Bay is considered as a net annual source for atmospheric CO2 in June and July, and the total CO2 flux from seawater into atmosphere is about 740t in June and 969 t in July.

关键词： DIC flux of CO_2 Jiaozhou Bay

Zonal propagation of kinetic energy and convection in the South china Sea and Indian monsoon regions in boreal summer

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Science china Earth sciences 2004年第12期47卷 1076-1084页

作者： CHEN Longxun 1 ,gao Hui 2 ,HE Jinhai 2 ,TAO Shiyan 3.& JIN Zuhui 3.1.chinese academy of Meteorological {3.,Beijing 100081,{3. 2.Nanjing Institute of Meteorology,Nanjing 210044,{3. 3.Institute of Atmospheric Physics,chinese academy of {3.,Beijing 100029,{3. 1. chinese academy of Meteorological sciences 100081 Beijing China 2. Nanjing Institute of Meteorology 210044 Nanjing China 3. chinese academy of sciences Institute of Atmospheric Physics 100029 Beijing China

Zonal propagation of kinetic energy (KE) and convection in the South china Sea (SCS) and Indian summer monsoon areas are examined in present study. Results suggest that the SCS and Indian summer monsoon prevailed regions (5 —15°N) are dominated by the southwesterly wind, however, the disturbances of KE at 850 hPa and convection are observed mainly coming from the western Pacific Ocean (140—150°E), after passing through the SCS, and westward propagated into the Bay of Bengal (90—100°E). In the Indian summer monsoon domain, where the disturbances of KE are found mainly coming from the Arabian Sea (AS) and eastward propagated into the Bay of Bengal. Therefore, the SCS and the Indian summer mon- soon are quite different in zonal propagation of KE and convection. The SCS summer monsoon is mainly affected by the KE and convection coming from the tropical western Pacific. The Indian summer monsoon, however, can be partly influenced by the AS and the SCS summer monsoon. The analysis also suggests that the interaction region between the SCS and the Indian summer monsoon is around 90—95°E, rather than 105°E as proposed by earlier studies.

关键词： zonal propagation of KE and convection, East Asian monsoon, Indian monsoon.

Entropy of three-dimensional BTZ black holes

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中国科学：物理学、力学、天文学英文版 2004年第3期47卷 277-283页

作者： gao Changjun1,2 & SHEN Yougen1,2,3.4 1.Shanghai Astronomical Observatory,chinese academy of {3.,Shanghai 20003.,{3.(mailing address) 2.National Astronomical Observatories,chinese academy of {3.,Beijing 100012,{3. 3.Institute of Theoretical Physics,chinese academy of {3.,Beijing 100080,{3. 4.Shanghai United Center for Astrophysics,Shanghai 20023.,{3. 1. Shanghai Astronomical Observatory Chinese Academy of Sciences 200030 Shanghai China 2. National Astronomical Observatories Chinese Academy of Sciences 100012 Beijing China 3. Institute of Theoretical Physics Chinese Academy of Sciences 100080 Beijing China 4. Shanghai United Center for Astrophysics 200234 Shanghai China

The entropies of scalar field and neutrino field are calculated in the background of three-dimensional BTZ black hole. Considering statistical physics,we propose not to consider the superraradiant modes for bosons (Fermion fields do not display supperradiance).In fact,the nonsuperradiant modes do contribute exactly the area entropy for both bosons and fermions. The result shows that the neutrino field entropy is 3.2 times the scalar one.

关键词： BTZ black holes, entropy, supperradiant modes.

Record-high efficiency perovskite LEDs achieved by accelerating radiative recombination

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Science china Materials 2024年第9期67卷 3039-3040页

作者： Feng gao Department of Physics Chemistry and BiologyLinköping UniversityLinköping58183Sweden

Light-emitting diodes(LEDs)have revolutionized the lighting and displays due to their superior energy *** increasing demand for more efficient and brighter thin-film LEDs in flat-panel displays and solid-state lighting applications has driven extensive research into perovskite materials,which show great promise for high brightness and efficiency.

关键词： lighting radiative high