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Influence of high-molecular-weight glutenin subunit deletions at the Glu-A1 and Glu-D1 loci on protein body development, protein components and dough properties of wheat(Triticum aestivum L.)

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Journal of Integrative Agriculture 2022年第7期21卷 1867-1876页

作者： LIU Da-tong ZHANG Xiao JIANG Wei LI Man WU Xu-jiang gao de-rong BIE Tong-de LU Cheng-bin Key Laboratory of Wheat Biology and Genetic Improvement for Low&Middle Yangtze Valley Ministry of Agriculture and Rural Affairs/Lixiahe Institute of Agricultural Sciences of JiangsuYangzhou 225007P.R.China

High-molecular-weight glutenin subunits(HMW-GSs) play a critical role in determining the viscoelastic properties of wheat. As the organelle where proteins are stored, the development of protein bodies(PBs) reflects the status of protein synthesis and also affects grain quality to a great extent. In this study, with special materials of four near-isogenic lines in a Yangmai 18 background we created, the effects of Glu-A1 and Glu-D1 loci deletions on the development and morphological properties of the protein body, protein components and dough properties were investigated. The results showed that the deletion of the HMW-GS subunit delayed the development process of the PBs, and slowed the increases of volume and area of PBs from 10 days after anthesis(DAA) onwards. In contrast, the areas of PBs at 25 DAA, the middle or late stage of endosperm development, showed no distinguishable differences among the four lines. Compared to the wild type and single null type in Glu-A1, the ratios of HMW-GSs to low-molecular-weight glutenin subunits(LMW-GSs), glutenin macropolymer(GMP) content, mixograph parameters as well as extension parameters decreased in the single null type in Glu-D1 and double null type in Glu-A1 and Glu-D1, while the ratios of gliadins(Gli)/glutenins(Glu) in those types increased. The absence of Glu-D1 subunits decreased both dough strength and extensibility significantly compared to the Glu-A1 deletion type. These results provide a detailed description of the effect of HMW-GS deletion on PBs, protein traits and dough properties, and contribute to the utilization of Glu-D1 deletion germplasm in weak gluten wheat improvement for use in cookies, cakes and southern steamed bread in China and liquor processing.

关键词： wheat near-isogenic lines HMW-GS deletion protein body dough property

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Marker-assisted selection to pyramid Fusarium head blight resistance loci Fhb1 and Fhb2 in the high-quality soft wheat cultivar Yangmai 15

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Journal of Integrative Agriculture 2023年第2期22卷 360-370页

作者： HU Wen-jing FU Lu-ping gao de-rong LI Dong-sheng LIAO Sen LU Cheng-bin Key Laboratory of Wheat Biology and Genetic Improvement for Low&Middle Yangtze Valley Ministry of Agriculture and Rural AffairsLixiahe Institute of Agricultural SciencesYangzhou 225007P.R.China College of Agronomy&Center for Crop Genome Engineering Henan Agricultural UniversityZhengzhou 45002P.R.China Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Agricultural College Yangzhou UniversityYangzhou 225009P.R.China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009P.R.China College of Agriculture Yangtze UniversityJingzhou 434023P.R.China

Fusarium head blight(FHB)is one of the most detrimental wheat diseases which greatly decreases the yield and grain quality,especially in the middle and lower reaches of the Yangtze River of ***1 and Fhb2 are two major resistance loci against Fusarium *** 15(YM15)is one of the most popular varieties in the middle and lower reaches of the Yangtze River,and it has good weak gluten characters but poor resistance to *** we used Fhb1 and Fhb2 to improve the FHB resistance of YM15 by a molecular marker-assisted selection(MAS)backcrossing *** selection of agronomic traits was performed for each *** successfully selected seven introgressed lines which carry homozygous Fhb1 and Fhb2 with significantly higher FHB resistance than the recurrent parent *** of the introgressed lines had agronomic and quality characters that were similar to *** study demonstrates that the pyramiding of Fhb1 and Fhb2 could significantly improve the FHB resistance in wheat using the MAS approach.

关键词： Fusarium head blight marker-assisted selection Fhb1 Fhb2 resistance breeding wheat

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Effect of high-molecular-weight glutenin subunit deletion on soft wheat quality properties and sugar-snap cookie quality estimated through near-isogenic lines

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Journal of Integrative Agriculture 2018年第5期17卷 1066-1073页

作者： ZHANG Xiao ZHANG Bo-qiao WU Hong-ya LU Cheng-bin Lü Guo-feng LIU Da-tong LI Man JIANG Wei SONG Gui-hua gao de-rong Key Laboratory of Wheat Biology and Genetic Improvement for Low and Middle Yangtze River Valley Wheat Region Ministry of Agriculture/Yangzhou Academy of Agricultural Sciences

High-molecular-weight glutenin subunits（HMW-GSs） play a critical role in determining the viscoelastic properties of wheat dough. The HMW-GSs are encoded by Glu-A1, Glu-B1, and Glu-D1 loci on the long arms of chromosomes 1A, 1B, and 1D, respectively. In the present study, four near-isogenic lines with different HMW-GS deletions and compositions at the Glu-A1 and Glu-D1 loci in Yangmai 18 background were used for quality analysis. deletion in Glu-D1 showed much weaker gluten quality and dough strength than null Glu-A1 genotype and wild genotype（WT）, based on the measurements of sodium dodecyl sulfate（SDS）-sedimentation, lactic acid solvent retention capacity（SRC）, gluten index, development time, stability time, and alveograph P and L values. The deletion of Glu-D1 did not significantly affect grain hardness, grain protein content, water SRC, sodium carbonate SRC, and sucrose SRC. Double null genotype in Glu-A1 and Glu-D1 and single null genotype in Glu-D1 showed significantly higher cookie diameter, crispness, and lower cookie height compared with single null genotype in Glu-A1 and WT. These indicate that the null Glu-D1 genotype is useful for improvement of biscuit quality, and use of this germplasm would be a viable strategy to develop new wheat varieties for biscuit processing.

关键词： wheat HMW-GS deletion near-isogenic lines cookie quality

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Direct and rapid detection of serum amino acid and monoamine neurotransmitters to assist the diagnosis of panic disorder

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Science China Chemistry 2025年第1期68卷 377-384页

作者： Shiwen Liu Xuerui Wan Meng Zhao Jiaqi Wang Weilan Wu Linlin You Yonggui Yuan Qian Xu rong gao Key Laboratory of Environmental Medicine Engineering Ministry of Education School of Public Health Southeast University Key Laboratory of Modern Toxicology of Ministry of Education Department of Hygienic Analysis and Detection School of Public HealthNanjing Medical University department of Psychosomatics and Psychiatry Zhongda Hospital School of Medicine Southeast University

The measurement of amino acid and monoamine neurotransmitters（NTs） in serum plays a central role in the identification and monitoring of clinical panic disorder（PD）. However, the strong polarity and chemical heterogeneity of NTs make direct extraction and rapid detection extremely challenging. Herein, we developed a strategy that integrated solid phase extraction based on magnetic Fe3O4coated with polydopamine（Fe3O4@PDA） and direct analysis of mass spectrometry in real time（DART-MS） for quantification of 10 NTs. We systematically investigated the optimal conditions of this strategy to achieve good accuracy and precision. Compared with existing magnetic solid phase extraction methods for NTs detection in biological samples, this strategy could adsorb more targets while having similar adsorption efficiencies, and the total process time was reduced by an average of 69.42%. We rapidly determined the metabolic abnormality of 6 types of NTs in the serum of PD patients and demonstrated their diagnostic accuracy. In addition, an outstanding feature of PD in which tryptophan was more metabolized by the KYN pathway and the 5-hydroxytryptamine pathway was inhibited could be elucidated by this strategy. Our results highlighted the potential ability of Fe3O4@PDA coupled with DART-MS to assist in the clinical diagnosis of PD.

关键词： neurotransmitters panic disorder magnetic solid phase extraction direct analysis of mass spectrometry in real time

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软质小麦溶剂保持力关联分析

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作物学报 2015年第2期41卷 251-258页

作者：张勇张晓郭杰高德荣张伯桥江苏里下河地区农业科学研究所/农业部长江中下游小麦生物学与遗传育种重点实验室江苏扬州225007

溶剂保持力（SRC）是软质小麦鉴定评价的重要指标。为获得与SRC关联的分子标记,提高育种效率,对不同硬度类型的176份品种的乳酸SRC、碳酸钠SRC、蔗糖SRC和水SRC进行SSR标记检测,并结合其在江苏里下河地区连续3个生长季的4种SRC表型数据... 详细信息

溶剂保持力（SRC）是软质小麦鉴定评价的重要指标。为获得与SRC关联的分子标记,提高育种效率,对不同硬度类型的176份品种的乳酸SRC、碳酸钠SRC、蔗糖SRC和水SRC进行SSR标记检测,并结合其在江苏里下河地区连续3个生长季的4种SRC表型数据,利用MLM模型进行了关联分析。以236对SSR引物共检测出1340个等位变异,平均每个位点5.5个等位变异,平均PIC值为0.4663。共检测到28个关联位点（P〈0.005）,单个位点可解释3.19%~21.84%的表型变异;与乳酸SRC、水SRC、蔗糖SRC和碳酸钠SRC相关联的位点分别为13、7、6和2个;与水SRC关联的gwm642-1D在3年中均被检测到。在这些关联位点上发现有利等位变异,其中降低水SRC的等位变异有gwm642-A186、gwm642-A188和gwm337-A178,降低蔗糖SRC的等位变异有gwm337-A178和gwm337-A186,降低碳酸钠SRC的等位变异有cfa2257-A129等。这些结果为利用分子标记进行SRC辅助选择提供了重要信息。

关键词：小麦溶剂保持力分子标记关联分析

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从十竹斋看水印木刻技艺的发展现状

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上海第二工业大学学报 2015年第1期32卷 68-73页

作者：高德荣上海第二工业大学应用艺术设计学院上海201209

明代末年,胡正言在金陵创立的十竹斋及其刻印出版的《十竹斋书画谱》、《十竹斋笺谱》可谓一举成名,备受当时文人追捧,亦对后世产生了重大影响。这两部精美绝伦的印刷艺术品因为水印木刻"饾版"、"拱花"印制技术的成熟使用而闻名遐迩。... 详细信息

明代末年,胡正言在金陵创立的十竹斋及其刻印出版的《十竹斋书画谱》、《十竹斋笺谱》可谓一举成名,备受当时文人追捧,亦对后世产生了重大影响。这两部精美绝伦的印刷艺术品因为水印木刻"饾版"、"拱花"印制技术的成熟使用而闻名遐迩。清代李渔的《芥子园画谱》后,水印木刻技术便逐渐式微,而研究木版水印技艺传承的现状无疑具有重大的历史意义和现实意义。从20世纪80年代开始,出现了大量研究《十竹斋书画谱》的文章,如王伯敏先生的《胡正言及其十竹斋的水印木刻》。到了21世纪的今天,木版水印以何种面貌传承、延续以及题材的变化发展等是该文的探讨主旨。

关键词：水印木刻十竹斋技艺传承

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会泽铅锌矿床成矿地质条件及找矿方向

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昆明理工大学学报（理工版） 2000年第4期25卷 19-24页

作者：高德荣云南会泽铅锌矿采选厂云南会泽654211

会泽麒麟厂矿床是赋存于早石炭世摆佐组碳酸盐岩中的层控富铅锌矿床 .研究表明 ,早石炭世摆佐组地层及其有利岩性和北东向断层与成矿关系密切 .在分析地层、岩相古地理、构造成矿地质条件的基础上 ,提出了找矿标志。

关键词：成矿地质条件找矿标志找矿方向铅锌矿床

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小麦育种策略探讨

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作物学报 2005年第7期31卷 932-939页

作者：程顺和张伯桥高德荣江苏里下河地区农科所江苏扬州225007

小麦育种的理论、方法与技术早有详尽的研究,但是综合运用它们的策略尚有待发展,因为育种理论与方法需有育种实践的长期积累,并与育种理论结合才具实际意义。笔者所在单位近50年来育成了一系列小麦品种,成为中华人民共和国成立以来长江... 详细信息

小麦育种的理论、方法与技术早有详尽的研究,但是综合运用它们的策略尚有待发展,因为育种理论与方法需有育种实践的长期积累,并与育种理论结合才具实际意义。笔者所在单位近50年来育成了一系列小麦品种,成为中华人民共和国成立以来长江下游及中游部分地区小麦品种3次大面积更换的主体品种,是小麦杂交育种的成功范例。笔者根据长期育种实践提出了小麦育种的若干策略。(1)品种改良过程必须以育种材料整体水平的提高为基础;(2)鉴定抉择时综合性状协调的观点是适应性育种的关键;(3)掌握好“苗期看长势”、“后期看熟相”、“考种看籽粒”3阶段表型选择方法;(4)采用同一地区创造不同生态条件和不同生态区多点鉴定结合,发挥自然选择与人工选择综合作用,以提高育种效率;(5)品种育成初期,存在种性再加工的可能性与必要性;(6)构建以大面积育种为基础、滚动回交与遗传标记结合的聚合育种为先导的高效育种体系是应用现代生物技术后的发展趋势。

关键词：小麦育种策略

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Bulging Performance and Quality Control of Aluminum Alloy Tailor-welded Overlapping Sheets Based on Interface Friction

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 258-264页

作者： gao Tiejun gao Bowen LI Weijie ZHANG Jiabin College of Aerospace Engineering Shenyang Aerospace UniversityShenyang 110136China Shenyang Aerospace Xinguang Group Co.L td. Shenyang 110043China

In order to solve the problem of poor formability caused by different materials and properties in the process of tailor-welded sheets forming,a forming method was proposed to change the stress state of tailor-welded sheets by covering the tailor-welded sheets with better plastic properties overlapping *** the same time,the interface friction effect between the overlapping and tailor-welded sheets was utilized to control the stress magnitude and further improve the formability and quality of the tailor-welded *** this work,the bulging process of the tailor-welded overlapping sheets was taken as the research *** alloy tailor-welded overlapping sheets bulging specimens were studied by a combination of finite element analysis and experimental *** results show that the appropriate use of interface friction between tailor-welded and overlapping sheets can improve the formability of tailor-welded sheets and control the flow of weld seam to improve the forming *** increasing the interface friction coefficient on the side of tailor-welded sheets with higher strength and decreasing that on the side of tailor-welded sheets with lower strength,the deformation of the tailor-welded sheets are more uniform,the offset of the weld seam is minimal,the limit bulging height is maximal,and the forming quality is optimal.

关键词： tailor-welded sheets overlapping sheet bulging interface friction weld seam stress

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Glu-A1和Glu-D1位点高分子量谷蛋白亚基共同缺失对弱筋小麦品质的影响

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麦类作物学报 2011年第3期31卷 450-454页

作者：武茹张晓高德荣别同德吴荣林程顺和扬州大学农学院江苏省作物遗传生理重点实验室教育部植物功能基因组学重点实验室江苏扬州225009 江苏里下河地区农业科学研究所江苏扬州225007

为了研究高分子量谷蛋白亚基(HMW-GS)缺失对小麦品质的影响,以Glu-A1和Glu-D1位点HMW-GS共同缺失材料2GS0414-10作供体亲本,以弱筋小麦品种扬麦13和扬麦18作轮回亲本进行回交,构建不同遗传背景的BC1F3和BC2F3群体,测定受体、供体亲本的... 详细信息

为了研究高分子量谷蛋白亚基(HMW-GS)缺失对小麦品质的影响,以Glu-A1和Glu-D1位点HMW-GS共同缺失材料2GS0414-10作供体亲本,以弱筋小麦品种扬麦13和扬麦18作轮回亲本进行回交,构建不同遗传背景的BC1F3和BC2F3群体,测定受体、供体亲本的品质,以及回交群体BC1F3和BC2F3中Glu-A1和Glu-D1位点HMW-GS共同缺失纯合单株及两位点均正常表达纯合单株的品质。结果表明,供体2GS0414-10具有较低的SDS沉降值、较短的面团形成时间和稳定时间;在BC1F3和BC2F3中,Glu-A1和Glu-D1位点HMW-GS共同缺失对蛋白含量影响不显著,但可显著或极显著降低SDS沉降值和水溶剂保持力(SRC)。Glu-A1和Glu-D1位点HMW-GS双缺失在弱筋小麦品质育种中具有一定的应用价值。

关键词：小麦 Glu-A1 Glu-D1 HMw-GS 双缺失品质

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