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Small extracellular vesicles derived from human induced pluripotent stem cell-differentiated neural progenitor cells mitigate retinal ganglion cell degeneration in a mouse model of optic nerve injury

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Neural Regeneration Research 2025年第2期20卷 587-597页

作者： Tong li Hui-Min Xing Hai-Dong Qian Qiao gao Sheng-Lan Xu Hua Ma Zai-Long Chi State Key Laboratory of Ophthalmology Optometry and Visual ScienceEye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina National Clinical Research Center for Ocular Diseases Eye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Several studies have found that transplantation of neural progenitor cells(NPCs)promotes the survival of injured ***,a poor integration rate and high risk of tumorigenicity after cell transplantation limits their clinical *** extracellular vesicles(sEVs)contain bioactive molecules for neuronal protection and *** studies have shown that stem/progenitor cell-derived sEVs can promote neuronal survival and recovery of neurological function in neurodegenerative eye diseases and other eye *** this study,we intravitreally transplanted sEVs derived from human induced pluripotent stem cells(hiPSCs)and hiPSCs-differentiated NPCs(hiPSC-NPC)in a mouse model of optic nerve *** results show that these intravitreally injected sEVs were ingested by retinal cells,especially those localized in the ganglion cell *** with hiPSC-NPC-derived sEVs mitigated optic nerve crush-induced retinal ganglion cell degeneration,and regulated the retinal microenvironment by inhibiting excessive activation of *** analysis further revealed that hiPSC-NPC derived sEVs transported neuroprotective and anti-inflammatory miRNA cargos to target cells,which had protective effects on RGCs after optic nerve *** findings suggest that sEVs derived from hiPSC-NPC are a promising cell-free therapeutic strategy for optic neuropathy.

关键词： exosome miRNA neural progenitor cell neurodegeneration neuroinflammation neuroprotection optic nerve crush optic neuropathy retinal ganglion cell small extracellular vesicles

Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

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Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： Yuan Zhao Yidan Zhang Xin liu Jian Zhang Ya gao Shuyue li Cui Chang Xiang liu Guofeng Yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

交叉引物扩增技术在肺外结核早期诊断中的价值

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中国生物制品学杂志 2016年第6期29卷 637-640页

作者：毛秀军葛晓励高景利河北省唐山市第四医院河北唐山063000 华北理工大学附属医院呼吸科河北唐山063000 河北省唐山市开滦总医院河北唐山063000

目的探讨交叉引物扩增技术(crossing priming amplification,CPA)在结核病诊断中的临床应用价值。方法对237例初治结核病患者标本(包括结核性腹膜炎70例,泌尿生殖道结核24例,结核性胸膜炎80例,骨结核13例,淋巴结核16例,结核性脑膜炎34例... 详细信息

目的探讨交叉引物扩增技术(crossing priming amplification,CPA)在结核病诊断中的临床应用价值。方法对237例初治结核病患者标本(包括结核性腹膜炎70例,泌尿生殖道结核24例,结核性胸膜炎80例,骨结核13例,淋巴结核16例,结核性脑膜炎34例,其中115例临床确诊为肺外结核)分别进行抗酸染色法、固体培养法及TBCPA法检测,以固体培养法和临床诊断结果为金标准,分析TB-CPA法检测标本中结核分枝杆菌的敏感度、特异度、阳性预测值、阴性预测值和Kappa值。结果以固体培养法和临床诊断结果为金标准,TB-CPA法以及抗酸染色法总体敏感性、特异性、阳性预测值、阴性预测值、Kappa值分别为91.86%(79/86)、87.42%(132/151)、80.61%(79/98)、92.05%(139/151)和0.81,以及53.49%(46/86)、96.03%(145/151)、88.46%(46/52)、122.52%(185/151)和0.54。结论 TB-CPA法可快速检测肺外结核分枝杆菌,具有良好的应用前景。

关键词：结核分枝杆菌交叉引物扩增肺外结核

老龄化背景下社区养老状况调查及对策:以杭州市为样本

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未来与发展 2015年第7期36卷 35-40,48页

作者：高丽静高凯东浙江理工大学马克思主义学院浙江杭州310018

随着我国人口老龄化程度加深,养老问题日渐凸显。在传统家庭养老功能弱化、机构养老无法满足需求的情况下,社区养老成为主流养老模式。通过对杭州市主城区各类老年人的问卷调查,了解当前老年人居家生活和社区养老的基本状况,提出强化社... 详细信息

随着我国人口老龄化程度加深,养老问题日渐凸显。在传统家庭养老功能弱化、机构养老无法满足需求的情况下,社区养老成为主流养老模式。通过对杭州市主城区各类老年人的问卷调查,了解当前老年人居家生活和社区养老的基本状况,提出强化社区功能、改善社区养老的对策建议,推动"老有所养、老有所乐"的小康社会品质社区的建设和完善。

关键词：社区养老状况调查对策建议

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汶川县高家沟泥石流堵江初步研究

四川地质学报 2016年第1期36卷 111-113,117页

作者：高精理四川省地质矿产勘查开发局成都水文地质工程地质中心成都610031

通过对汶川县高家沟泥石流2011年"7·3"泥石流运动特征分析,结合泥石流堵江经验公式计算不同暴雨条件下泥石流堵江的可能性,认为"5·12"地震后当降雨频率达到20年一遇时会对岷江河道产生影响,随着降雨频率的降低,对河道影响越大。

关键词：泥石流堵江研究高家沟

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焦化行业水污染防治问题与对策研究

煤 2006年第2期15卷 41-42页

作者：高景丽长治市环境保护局山西长治046000

对焦化废水污染防治的现状进行了分析评价,针对存在的问题提出了对策措施。焦化行业废水处理率低、工艺落后、部分设施质量差、管理落后、关键技术成本高,对此,应从执法、管理、清洁生产、扶持新技术、降低成本几个方面加以解决。

关键词：焦化废水污染防治

Passive activity enhances residual control ability in patients with complete spinal cord injury

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Neural Regeneration Research 2025年第8期20卷 2337-2347页

作者： Yanqing Xiao Mingming gao Zejia He Jia Zheng Hongming Bai Jia-Sheng Rao Guiyun Song Wei Song Xiaoguang li Beijing Key Laboratory for Biomaterials and Neural Regeneration Beijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijingChina Department of Rehabilitation Evaluation China Rehabilitation Research CenterBeijingChina Cardiac Center Beijing Children’s HospitalCapital Medical UniversityBeijingChina The State Key Laboratory of Virtual Reality Technology and Systems Beihang UniversityBeijingChina Department of Rehabilitation Engineering China Rehabilitation Science InstituteBeijingChina

Patients with complete spinal cord injury retain the potential for volitional muscle activity in muscles located below the spinal injury ***,because of prolonged inactivity,initial attempts to activate these muscles may not effectively engage any of the remaining neurons in the descending pathway.A previous study unexpectedly found that a brief clinical round of passive activity significantly increased volitional muscle activation,as measured by surface *** this study,we further explored the effect of passive activity on surface electromyographic signals during volitional control tasks among individuals with complete spinal cord *** patients with chronic complete thoracic spinal cord injury were *** electromyography data from eight major leg muscles were acquired and compared before and after the passive activity *** results indicated that the passive activity led to an increased number of activated volitional muscles and an increased frequency of *** the cumulative root mean square of surface electromyography amplitude for volitional control of movement showed a slight increase after passive activity,the difference was not statistically *** findings suggest that brief passive activity may enhance the ability to initiate volitional muscle activity during surface electromyography tasks and underscore the potential of passive activity for improving residual motor control among patients with motor complete spinal cord injury.

关键词： complete spinal cord injury cycle training epidural electrical stimulation motor training passive activity physiological state spinal cord circuit surface electromyography volitional control task

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论上市公司绩效评价的重要性及方法

绵阳师范学院学报 2004年第6期23卷 28-31页

作者：高景丽绵阳师范学院经济系四川绵阳621000

本文在借鉴国内外企业绩效评价理论的基础上,从现代财务管理的角度,结合相关学科理论,探讨上市公司绩效评价的重要性及方法。上市公司绩效评价的重要性源于证券市场快速发展,各利益相关方需要一个科学的评价尺度。对一个上市公司的绩... 详细信息

本文在借鉴国内外企业绩效评价理论的基础上,从现代财务管理的角度,结合相关学科理论,探讨上市公司绩效评价的重要性及方法。上市公司绩效评价的重要性源于证券市场快速发展,各利益相关方需要一个科学的评价尺度。对一个上市公司的绩效评价应从三方面展开:公司基本素质分析,会计数据分析,财务状况分析。

关键词：上市公司企业绩效评价会计数据证券市场外企财务管理财务状况分析重要性相关学科基本素质

水文地质在矿山地质勘查中的重要性及有效措施探讨

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世界有色金属 2023年第18期 109-111页

作者：高精理四川省地质环境调查研究中心四川成都610081

在我国工业现代化发展进程不断加快的形势下,人们日常生产生活中对于矿产资源的需求也越来越大。但是,要想持续合理、有效的开采矿山资源,就需要对前期的矿山地质勘查工作予以高度的重视。水文地质是矿山地质勘查工作中最关键的一个环节... 详细信息

在我国工业现代化发展进程不断加快的形势下,人们日常生产生活中对于矿产资源的需求也越来越大。但是,要想持续合理、有效的开采矿山资源,就需要对前期的矿山地质勘查工作予以高度的重视。水文地质是矿山地质勘查工作中最关键的一个环节,对于整个矿山地质勘查的工作质量有着直接的影响。本文重点针对水文地质在矿山地质勘查中的重要性及有效措施进行了详细的分析,以供参考。

关键词：矿山地质勘查水文地质重要性措施