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Histochemical pattern of gastrocnemius muscle in fluoride toxicity syndrome

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Asian Pacific Journal of Tropical Medicine 2010年第2期3卷 136-140页

作者： Shashi A bhushan b bhardwaj M Department of Zoology Punjabi University

Objective:To evaluate the effect of sodium fluoride(NaF) on cytochemical distribution of proteins,dna,and RNA in the gastrocnemius muscle of rat in experimental ***: Young Sprague Dawley albino rats were administered with NaF at 30,45,and 75 mg/kg bw/ day subcutaneously for 15 and 30 days,*** control animals were given the vehicle(1cc deionized double distilled water/kg bw/day).Results:In the first phase of 15 days experimentation,the gastrocnemius muscle of rats intoxicated with NaF at 30,45,and 75 mg /kg bw/ day showed decline in proteins including a amino acids as compared to control. In the second phase of 30 days experimentation,the muscle fibers of rat showed elevation in sarcolemmal and sarcoplasmic proteins in 30 mg NaF dose group,and angulated fibers exhibited increase in sarcolemmal proteins in 45 mg NaF *** marginal regions of angular and rim fibers showed deeply stained rings of sarcoplasmic proteins whereas the split fibers were faindy stained in rats treated with NaF at 75 mg/kg bw/day for 30 *** rats treated with NaF at 30 mg/kg bw/day for 15 days,the hypertrophied peripheral muscle fibers contained more dna,however the atrophied fibers had more *** 45 mg NaF group,RNA was located in sarcolemmal regions,while dna content decreased as compared to *** 75 mg NaF group,the muscle fibers had dark and light staining regions of dna and *** the 30 days of experimentation,the dna level decreased whereas RNA content increased in the gastrocnemius muscle fibers of the rat treated with NaF at 30 mg/kg bw/*** treatment group with NaF at 45 mg/kg bw/day,the RNA content slighdy declined in comparison to control,in all treatments for 15 days as well as in treatment group with NaF at 30 mg/kg bw/day for 30 days whereas the amount of dna slightly increased as compared to treatment group with NaF at 75 mg/kg bw/day for 15 *** highest dose group revealed elevated amount of RNA whereas dna content remained stable.C

关键词： Albino rats dna gastrocnemius muscle Proteins RNA Sodium fluoride

Fragile X mental retardation protein interacts with TDg

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Chinese Science bulletin 2000年第6期45卷 516-520页

作者： bardoni b MandelJ.-L IgbMC CNRS-INSERM-ULP67404 IllkirchFrance Correspondence should be addressed to Shen YanIGBMCCNRS-INSERM-ULP67404 IllkirchFrance Correspondence Should be addressed to Shen Yan

Fragiie X syndrome is the most common form of inherited mental retardation disease, resulting from absent of expression of its disease gene FMR1. To study the function of the fragiie X mental retardation protein (FMRP) through protein/protein interaction, a mouse embryo cdna library was screened by the yeast two-hybrid system. A clone was found to interact specifically with FMRP. The cdna of this clone ( genbank accession number af102875 ) encoded a protein highly homologous to human g/T mismatch-specific dna thymine glycosylase ( hTDg ). Interactions between various alternatively spliced FMRP isoforms and a series of mTDg deletion proteins were further studied in the yeast two-hybrid system and their interaction amino acid regions were determined. interaction between FMRP and TDg existed inside exon 13 of FMRP ( amino acid residue 397-425 ) and around amino acid residue 122-346 of TDg. These results will be helpful to the study of the biological role of FMRP.

关键词： fragile X mental retardation protein g/T mismatch-specific dna thymine glycosylase yeast two-hybrid protein/protein interaction.

Treatment of chronic viral hepatitis with nitazoxanide and second generation thiazolides

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World Journal of gastroenterology 2009年第15期15卷 1805-1808页

作者： Emmet b Keeffe Jean-Franois Rossignol The Romark Institute for Medical Research TampaFlorida and SausalitoCaliforniaUnited States and the Division of Gastroenterology and HepatologyDepartment of MedicineStanford University Medical Center The Romark Institute for Medical Research TampaFloridaUnited States and the Division of Gastroenterology and HepatologyDepartment of MedicineStanford University Medical Center

Nitazoxanide,the first thiazolide,was originally developed for the treatment of Cryptosporidium *** recently,antiviral activity of nitazoxanide against hepatitis b virus(HbV)and hepatitis C virus was recognized in in vitro *** basic studies led to phaseⅡclinical trials that demonstrated the safety and efficacy of nitazoxanide in combination with peginterferon,with or without ribavirin,in the treatment of chronic hepatitis C genotype *** sustained virologic response rate was 79%and 80%in two studies,which was higher than the response rate of 50%with the standard of care with peginterferon plus *** very preliminary studies of patients with chronic hepatitis b,nitazoxanide suppressed serum HbV dna and led to loss of hepatitis b e antigen in the majority of patients and hepatitis b surface antigen in approximately a quarter of *** controlled studies of naive and nonresponder patients with chronic hepatitis C genotype 1 are underway,new second generation and controlled release thiazolides are being developed,and future studies of patients with chronic hepatitis b are planned.

关键词： Hepatitis C Hepatitis C virus Nitazoxanide

TELS: A Novel Computational Framework for Identifying Motif Signatures of Transcribed Enhancers

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genomics, Proteomics & bioinformatics 2018年第5期16卷 332-341页

作者： Dimitrios Kleftogiannis Haitham Ashoor Vladimir b.bajic Centre for Evolution and Cancer Division of Molecular PathologyThe Institute of Cancer Research(ICR)London SM2 5NGUnited Kingdom The Jackson Laboratory for genomic Medicine FarmingtonCT 06032USA Computational bioscience Research Center(CbRC) ComputerElectrical and Mathematical Sciences and Engineering

In mammalian cells, transcribed enhancers(TrEns) play important roles in the initiation of gene expression and maintenance of gene expression levels in a spatiotemporal manner. One of the most challenging questions is how the genomic characteristics of enhancers relate to enhancer activities. To date, only a limited number of enhancer sequence characteristics have been investigated, leaving space for exploring the enhancers’ dna code in a more systematic way. To address this problem, we developed a novel computational framework, Transcribed Enhancer Landscape Search(TELS), aimed at identifying predictive cell type/tissue-specific motif signatures of *** a case study, we used TELS to compile a comprehensive catalog of motif signatures for all known TrEns identified by the FANTOM5 consortium across 112 human primary cells and *** results confirm that combinations of different short motifs characterize in an optimized manner cell type/tissue-specific TrEns. Our study is the first to report combinations of motifs that maximize classification performance of TrEns exclusively transcribed in one cell type/tissue from TrEns exclusively transcribed in different cell types/tissues. Moreover, we also report 31 motif signatures predictive of enhancers’ broad activity. TELS codes and material are publicly available at http://***/TELS.

关键词： Sequence analysis Machine learning Transcription regulation Transcribed enhancer Motif identification

FERONIA Is a Key Modulator of brassinosteroid and Ethylene Responsiveness in Arabidopsis Hypocotyls

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Molecular Plant 2010年第3期3卷 626-640页

作者： Stephen D. Deslauriers Paul b. Larsen Department of biochemistry University of California-Riverside Riverside CA 92521 USA

Ethylene signaling is a complex pathway that has been intensively analyzed partly due to its importance to the manifestation of horticultural phenomena, including fruit ripening and tissue senescence. In order to further our un- derstanding of how this pathway is regulated, a screen for Arabidopsis mutants with increased ethylene response was conducted. From this, a mutant was identified as having a dark-grown hypocotyl that is indistinguishable from Col-0 wt in the presence of the ethylene perception inhibitor AgNO3, yet has extreme responsiveness to even low levels of ethylene. Map-based cloning of the mutation revealed a T-dna insertion in the coding sequence of the receptor-like kinase FERONIA, which is required for normal pollen tube reception and cell elongation in a currently unknown capacity. In contrast to a previous report, analysis of our feronia knockout mutant shows it also has altered responsiveness to brassinosteroids, with etiolated fer-2 seedlings being partially brassinosteroid insensitive with regard to promotion of hypocotyl elonga- tion. Our results indicate that FERONIA-dependent brassinosteroid response serves to antagonize the effect of ethylene on hypocotyl growth of etiolated seedlings, with loss of proper brassinosteroid signaling disrupting this balance and leading to a greater impact of ethylene on hypocotyl shortening.

关键词： FERONIA ethylene brassinosteroid triple response hypocotyl Arabidopsis.

The complex of Diplodia species associated with Fraxinus and some other woody hosts in Italy and Portugal

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Fungal Diversity 2014年第4期67卷 143-156页

作者： A.Alves b.T.Linaldeddu A.Deidda b.Scanu A.J.L.Phillips Departamento de biologia CESAMUniversidade de Aveiro3810-193 AveiroPortugal Dipartimento di Agraria Sezione di Patologia vegetale ed EntomologiaUniversitàdegli Studi di Sassarivia E.De Nicola 107100 SassariItaly Centro de Recursos Microbiológicos Departamento de Ciencias da VidaFaculdade de Ciencias e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal

Studies on the taxonomy and phylogeny of Diplodia have been hampered by the lack of an ex-type culture linked to the holotype of ***,which is the type of the *** this study a large collection of Diplodia strains,obtained from ash and other woody hosts showing V-shaped cankers and branch dieback,were identified based on morphological characters and dna sequence data from ITS and EF1-α *** of combined morphological and phylogenetic analyses showed that the Fraxinus isolates from Italy,the Netherlands,Portugal and Spain belong to three distinct species namely Diplodia fraxini,Diplodia mutila and Diplodia subglobosa *** epitype was designated for Diplodia mutila,with associated ex-epitype *** name *** is re-instated and a neotype *** species,Diplodia seriata and Diplodia pseudoseriata were reported for the first time on Fraxinus spp.

关键词： botryosphaeriaceae Epitype Neotype Phylogeny Systematics Taxonomy

dna damage, NF-κb and accelerated aging

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Asian Journal of Andrology 2012年第6期14卷 811-812页

作者： David g Le Couteur David J Handelsman ANZAC Medical Research Institute Sydney 2139 Australia Centre for Education and Research on Ageing (CERA) Concord Hospital and University of Sydney Concord NSW 2139 Australia

The aging process is the major risk factor for disease and disability yet the cel- lular mechanisms for aging are uncertain.

关键词： LIFE-SPAN NUCLEAR AbNORMALITIES MICE

The Value and Significance of Metagenomics of Marine Environments

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genomics, Proteomics & bioinformatics 2015年第5期13卷 271-274页

作者： Fangqing Zhao Vladimir b.bajic Computational genomics Lab Beijing Institutes of Life ScienceChinese Academy of Sciences Computational bioscience Research Center (CbRC) ComputerElectrical and Mathematical Sciences and Engineering Division (CEMSE)King Abdullah University of Science and Technology (KAUST)

Microbes have played a fundamental role in the natural his- tory of our planet, and have done so for billions of years. They have adapted to Earth＇s many environments from the mild to the very extreme. Studying their diversity and their way of life is critical for understanding their full impact on the global ecology. Although the field of metagenomics is still young, it has unravelled a wider microbial diversity that had otherwise been completely overlooked by the traditional methods of microbiology. There are three major metagenomic themes：（a） marker metagenomics that surveys microbial community structure by targeting the highly-conserved 16S rRNA gene, （b） functional metagenomics that takes the total environmental dna, from which it infers the metabolic potential of the microbial community, and （c） identification of novel enzymes.

关键词： The Value and Significance of Metagenomics of Marine Environments

genotypic characteristics of resistant tumors to pre-operative ionizing radiation in rectal cancer

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World Journal of gastrointestinal Oncology 2014年第7期6卷 194-210页

作者： Zeeshan Ramzan Ammar b Nassri Sergio Huerta VA North Texas Healthcare System-Dallas VA Medical Center University of Texas Southwestern Medical Center Dallas TX 75216 United States

Due to a wide range of clinical response in patients un-dergoing neo-adjuvant chemoradiation for rectal cancer it is essential to understand molecular factors that lead to the broad response observed in patients receiving the same form of *** extensive research in this field,the exact mechanisms still remain *** raging from dna-repair to specific molecules lead-ing to cell survival as well as resistance to apoptosis have been ***,or in combination,there is no single pathway that has become clinically applicable to *** the following review,we describe the current status of various pathways that might lead to resistance to the therapeutic applications of ionizing radiation in rectal cancer.

关键词： Ionizing radiation dna double-strand break Non-homologous end-joining pathway dna-PKcs Ku proteins Complete pathological response Radiation therapy Apoptosis Angiogenesis