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Succession of Bacterial Community Inhabited Acid Mine Drainage under High Fe（Ⅱ） Concentration

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Journal of Environmental Science and Engineering 2010年第8期4卷 46-55页

作者： M.X. Wan Y. Yang W.M. zeng J.L. Xia X.D. Liu W.Q. Qin g.z. qiu School of Minerals Processing and Bioengineering Central South University Hunan 410083 China Department of Chemical ＆ Biomolecular Engineering Johns Hopkins University MD 21218 USA Key Laboratory of Biometalhtrgy of Ministry of Education Hunan 410083 China CSIRO Process Science and Engineering Clayton VIC 3168 Australia

To reveal the effects of Fe2＋ on bacterial communities in the early stages of minerals dissolution, two different acid mine drainage （AMD） samples were collected at Dabaoshan Mine and Shenbu Mine. Community successions of AMD niches were analyzed by Amplified Ribosomal DNA Restriction Analysis （ARDRA）, sequencing, and phylogenetic analysis in original AMD samples and their subculture under Fe2＋ concentrations. Although geochemical properties and community structures were greatly different between the two original AMD samples, bacterial community successions were still very similar under high Fe2＋ concentrations. The results showed that Acidithiobacillus ferrooxidans have competitive relationship with other bacterial species living in the AMD, including species that were also capable of oxidizing ferrous ion. A competitive relationship among different At. ferrooxidans strains likewise existed. Some of At. ferrooxidans can grow first under conditions of high ferrous ion concentration, and other At. ferrooxidans species decreased gradually and disappeared. This suggested that these species of At. ferrooxidans are most acidophilic bacteria and afford Fe3＋ to leach other metallic ion in the early stages of minerals dissolution.

关键词： Bacterial diversity succession acid mine drainage amplified ribosomal DNA restriction analysis.

Macro-microscopic dynamic characteristics of the ballast bed induced by the variation of moisture content

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Particuology 2024年第7期90卷 118-127页

作者： Shuchen Wang Longlong Fu Yongjia qiu Haonan Xi Shunhua zhou Key Laboratory of Road and Traffic Engineering of the Ministry of Education Tongji UniversityShanghai 201804China Shanghai Key Laboratory of Rail Infrastructure Durability and System Safety Tongji UniversityShanghai 201804China

Due to the excellent drainage performance of the ballast,existing studies mainly focus on the dynamic response of ballast under field capacity or *** has rarely been paid to dynamic changes in moisture content and potential *** this article,we firstly conduct a model test to determine the variation of ballast moisture content under artificial *** that,a full-scale model test with cyclic loading is carried out to study the effect of moisture content variation on the macro-microscopic response of the ballast bed,where several wireless particle sensors are installed to obtain ballast motion characteristics at strategic *** results show that the moisture content increases gradually and stabilizes at a flat peak under rainfall,despite the excellent drainage performance of ballast *** halting rainfall,the moisture content drops back to field capacity,which indicates dynamic flowing surface water on ballast particles under *** flowing surface water brings changes to the original dynamic equilibrium of ballast bed:macroscopically,the deformation rate of stabilized ballast bed increases significantly,reaching a local peak under field capacity;microscopically,the x-and z-angular accelerations of the ballast show positive correlation with rainfall *** multiscale responses indicate that field capacity is a critical moisture content.

关键词： Railway ballast Flowing surface water Wireless particle sensor Multidimensional motion Full-scale model test

Coupling coordinated development among digital economy,regional innovation,and talent employment:A case study in the Hangzhou Metropolitan Circle,China

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Regional Sustainability 2024年第1期5卷 93-106页

作者： qiu Luyi Sharina OSMAN HUA Yidi Department of International Trade and Economics Ningbo PolytechnicNingbo315800China Business School Universiti Kuala LumpurKuala Lumpur50250Malaysia Research Institute of Digital Culture and Tourism Ningbo PolytechnicNingbo315800China School of International Studies University of Nottingham Ningbo ChinaNingbo315400China

Coordinative development across various systems,particularly the economic,social,cultural,and human resources subsystems,is a key aspect of urban sustainability and has a direct impact on the quality of *** Hangzhou Metropolitan Circle,comprising Hangzhou City,Huzhou City,Jiaxing City,and Shaoxing City,was the first metropolitan circle approved by the National Development and Reform Commission(NDRC)as a demonstration of economic transformation in *** evaluate the coupling coordination degree of the four cities and analyze the coordinative development in three systems(including digital economy,regional innovation,and talent employment),we collected panel data during 2015–2022 from these four *** development level of the three systems was evaluated by the standard deviation method and comprehensive development *** results are as follows:(1)the level of coupling coordinated development of the three systems in the Hangzhou Metropolitan Circle was relatively low;(2)the coupling coordination degree of the four cities in the Hangzhou Metropolitan Circle showed significant regional differences,among which Hangzhou City was in the leading position,and Huzhou,Jiaxing,and Shaoxing cities made steady but slow progress in the coupling development of the three systems;and(3)the development of digital economy and talent employment needs to be *** study contributes to the coordinative development of Hangzhou Metropolitan Circle by innovatively focusing on the coupling coordination relationship among digital economy,regional innovation,and talent employment,which also meets the industrial layout of Hangzhou Metropolitan *** this way,the optimal allocation and sustainable development of digital economy,regional innovation,and talent employment in the Hangzhou Metropolitan Circle can be achieved.

关键词： Digital economy Regional innovation Talent employment Coupling coordination degree Hangzhou Metropolitan Circle

Lath-like phases formed at an extremely high temperature in a Mg-RE(RE=rare earth)-Al alloy

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Rare Metals 2024年第8期43卷 3937-3945页

作者： Bo Deng Shu-Hui Lv Qiang Yang Dong-Yue zhao Jun-Yuan Fan Xin qiu School of Materials Science and Engineering Changchun University of Science and TechnologyChangchun 130022China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Chongqing Research Institute Changchun University of Science and TechnologyChongqing 401135China

Thermal stable intermetallic particles are important for the heat resistance of magnesium(Mg)*** this work,many lath-like particles formed in α-Mg grains of a Mg-8gd-3Sm-0.7Al casting alloy when heat-treated at 873 ***-resolution high-angle annular dark field scanning transmission electron microscopy(HAADF-STEM) characterizations indicate that most of them are Mg-containing Al_(2)(gd,Sm),with the atomic ratio of Mg:Al:(gd,Sm) being ~1:1:1;a small part of them with relatively wider thickness are long-period stacking ordered(LPSO) phases simultaneously containing both 14H and 18R *** followed common orientation relationships with Mg matrix as those reported in previous *** addition,many Mg laths were observed in the primary blocky Al_(2)(gd,Sm) phase at grain boundaries,where the atomic ratio of Al:(gd,Sm) in the Al_(2)(gd,Sm) matrix was 2:***,density functional theory(DFT) calculations illustrated the detail structure of the re-constructed Mg/Al_(2)RE interface and simultaneously deduced the underlying reason for the re-dissolution of the newly formed Mg-containing Al_(2)(gd,Sm) plates in α-Mg matrix.

关键词： Magnesium alloy HAADF-STEM Microstructures Intermetallic particles Orientation relationships Interfaces

Normalized glandular dose coefficients for digital breast tomosynthesis using detailed Chinese breast models

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Nuclear Science and Techniques 2024年第5期35卷 42-59页

作者： Jia-Hao Wang Rui qiu An-Kang Hu Ye-Qi Liu zhen Wu Hui zhang Jun-Li Li Department of Engineering Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Particle and Radiation Imaging Ministry of EducationTsinghua UniversityBeijing 100084China Joint Institute of Tsinghua University Nuctech Company Limited BeijingBeijing 100084China

The rise in breast cancer diagnoses among Chinese women has necessitated the use of X-ray breast screening,which carries a radiation *** study aimed to provide a dosimetry protocol for the Chinese female population to replace the traditional standard that utilizes simplified breast models,for the accurate estimation of the mean glandular dose of a patient undergoing digital breast tomosynthesis(DBT).The first set of detailed Chinese female breast models and representative breast parameters was *** backscatter radiation and computational efficiency,we improved the combination of these models and the Chinese reference adult female whole-body voxel *** acquisition for four commercial DBT systems that are widely employed in China was simulated using the Monte Carlo method to obtain the normalized glandular dose coefficients of DBT(D_(gN)^(DBT))and the glandular depth dose(D_(g)^(dep)(z))for different breast characteristics and X-ray *** calculated a series of D_(gN)^(DBT) values for breasts with different percentage mass glandularities(5%,25%,50%,75%,and 100%)and compressed breast thicknesses(2,3,4,5,6,and 7 cm)at various tube potentials(25,28,30,32,35,and 49 kV)and target/filter combinations(W/Rh,W/Al,Mo/Mo,Rh/Rh,and Rh/Ag).The parameter dependence of the breast characteristics and beam conditions on D_(gN)^(DBT) in detailed breast models was *** D_(gN)^(DBT) results were 14.6-51.0%lower than those of the traditional dosimetry standard in *** difference in D_(gN)^(DBT) was mainly due to a decrease in the depth of the main energy deposition area caused by the glandular distribution along the depth *** results obtained in this study may be used to improve breast dosimetry in China and provide more detailed information on risk assessment during DBT.

关键词： Digital breast tomosynthesis Normalized glandular dose coefficients Detailed breast model Monte Carlo simulation

Design of antiviral AgO2-dependent short hairpin RNAs

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Virologica Sinica 2024年第4期39卷 645-654页

作者： Yuanyuan Bie Jieling.z.ang Jiyao Chen Yumin zhang Muhan Huang Leike zhang Xi zhou Yang qiu Key Laboratory of Virology and Biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China School of Life Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China

The increasing emergence and re-emergence of RNA virus outbreaks underlines the urgent need to develop effective *** interference(RNAi)is a sequence-specific gene silencing mechanism that is triggered by small interfering RNAs(siRNAs)or short hairpin RNAs(shRNAs),which exhibits significant promise for antiviral ***2-dependent shRNA(agshRNA)generates a single-stranded guide RNA and presents significant advantages over traditional siRNA and *** this study,we applied a logistic regression algorithm to a previously published chemically siRNA efficacy dataset and built a machine learning-based model with high predictive *** this model,we designed siRNA sequences targeting diverse RNA viruses,including human enterovirus A71(EV71),zika virus(zIKV),dengue virus 2(DENV2),mouse hepatitis virus(MHV)and severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),and transformed them into *** validated the performance of our agshRNA design by evaluating antiviral efficacies of agshRNAs in cells infected with different *** the agshRNA targeting EV71 as an example,we showed that the anti-EV71 effect of agshRNA was more potent compared with the corresponding siRNA and ***,the antiviral effect of agshRNA is dependent on AgO2-processed guide RNA,which can load into the RNA-induced silencing complex(RISC).We also confirmed the antiviral effect of agshRNA in ***,this work develops a novel antiviral strategy that combines machine learning-based algorithm with agshRNA design to custom design antiviral agshRNAs with high efficiency.

关键词： AgshRNA Antiviral strategy Machine learning model

Structural insights into AtABCg25,an angiosperm-specific abscisic acid exporter

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Plant Communications 2024年第1期5卷 284-295页

作者： Jian Xin Yeling.z.ou Yichun qiu He geng Yuzhu Wang Yi Song Jiansheng Liang Kaige Yan Department of Chemical Biology School of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China Institute of Plant and Food Science Department of BiologySchool of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China Max Planck Institute of Molecular Plant Physiology Potsdam-GolmGermany Institute for Biological Electron Microscopy Southern University of Science and TechnologyShenzhenGuangdongChina

Cellular hormone homeostasis is essential for precise spatial and temporal signaling responses and *** acid(ABA)plays pivotal roles in orchestrating various developmental and stress re-sponses and confersfitness benefits over ecological and evolutionary timescales in terrestrial *** ABA level is regulated by complex processes,including biosynthesis,catabolism,and ***25 is thefirst ABA exporter identified through genetic screening and affects diverse ABA *** the structural basis of ABA export by ABCg25 is critical for further manipulations of ABA homeostasis and *** used cryo-electron microscopy to elucidate the structural dynamics of AtABCg25 and successfully characterized different states,including apo AtABCg25,ABA-bound AtABCg25,and ATP-bound AtABCg25(E232Q).Notably,AtABCg25 forms a homodimer that fea-tures a deep,slit-like cavity in the transmembrane domain,and we precisely characterized the critical residues in the cavity where ABA *** binding triggers closure of the nucleotide-binding domains and conformational transitions in the transmembrane *** show that AtABCg25 belongs to a conserved ABCg subfamily that originated during the evolution of *** subfamily neofunctionalized to regulate seed germination via the endosperm,in concert with the evolution of this angio-sperm-specific,embryo-nourishing ***,thesefindings provide valuable insights into the intricate substrate recognition and transport mechanisms of the ABA exporter AtABCg25,paving the way for genetic manipulation of ABA homeostasis and plantfitness.

关键词： AtABCg25 abscisic acid ABC transporters cryo-EM structure

Evaluating eDNA and eRNA metabarcoding for aquatic biodiversity assessment: From bacteria to vertebrates

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Environmental Science and Ecotechnology 2024年第5期21卷 396-404页

作者： Yan zhang Yu qiu Kai Liu Wenjun zhong Jianghua Yang Florian Altermatt Xiaowei zhang State Key Laboratory of Pollution Control&Resource Reuse School of the EnvironmentNanjing UniversityNanjing210023China Department of Evolutionary Biology and Environmental Studies University of ZurichZürichSwitzerland Department of Aquatic Ecology Eawag:Swiss Federal Institute of Aquatic Science and TechnologyDübendorfSwitzerland Key Laboratory of Freshwater Fisheries and germplasm Resources Utilization Ministry of Agriculture and Rural AffairsFreshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxi214081China

The monitoring and management of aquatic ecosystems depend on precise estimates of *** analyses of environmental nucleic acids(eNAs),including environmental DNA(eDNA)and environmental RNA(eRNA),have garnered attention for their cost-effective and non-invasive biomonitoring ***,the accuracy of biodiversity estimates obtained through eNAs can vary among different organismal *** we evaluate the performance of eDNA and eRNA metabarcoding across nine organismal groups,ranging from bacteria to terrestrial vertebrates,in three crosssections of the Yang.z. River,*** observe robust complementarity between eDNA and eRNA *** relative detectability of eNAs was notably influenced by major taxonomic groups and organismal sizes,with eDNA providing more robust signals for larger *** eDNA and eRNA exhibited similar cross-sectional and longitudinal ***,the detectability of larger organisms declined in eRNA metabarcoding,possibly due to differential RNA release and decay among different organismal groups or *** underscoring the potential of eDNA and eRNA in large river biomonitoring,we emphasize the need for differential interpretation of eDNA versus eRNA *** highlights the importance of careful method selection and interpretation in biomonitoring studies.

关键词： Environmental nucleic acids Aquatic biodiversity Organismal size Species detectability

End-group modulation of phenazine based non-fullerene acceptors for efficient organic solar cells with high open-circuit voltage

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Journal of Energy Chemistry 2024年第1期88卷 461-468,I0011页

作者： Yahui zhang Yafeng Li Ruixiang Peng Yi qiu Jingyu Shi zhenyu Chen Jinfeng ge Cuifen zhang.z.eng Tang.z.yi ge School of Materials Science and Engineering Shanghai UniversityShanghai 200444China zhejiang Business Technology Institute Ningbo 315012ZhejiangChina zhejiang Provincial Engineering Research Center of Energy Optoelectronic Materials and Devices Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201ZhejiangChina Center for Advanced Low-dimension Materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China

Phenazine-based non-fullerene acceptors(NFAs)have demonstrated great potential in improving the power conversion efficiency(PCE)of organic solar cells(OSCs).Halogenation is known to be an effective strategy for increasing optical absorption,refining energy levels,and improving molecular packing in organic ***,a series of NFAs(Pz IC-4H,Pz IC-4F,Pz IC-4Cl,Pz IC-2Br)with phenazine as the central core and with/without halogen-substituted(dicyanomethylidene)-indan-1-one(IC)as the electron-accepting end group were synthesized,and the effect of end group matched phenazine central unit on the photovoltaic performance was systematically *** photophysical and morphological analyses revealed that the PM6:Pz IC-4F blend involves efficient exciton dissociation,higher charge collection and transfer rates,better crystallinity,and optimal phase ***,OSCs based on PM6:Pz IC-4F as the active layer exhibited a PCE of 16.48%with an open circuit voltage(Voc)and energy loss of 0.880 V and 0.53 e V,***,this work demonstrated a promising approach by designing phenazine-based NFAs for achieving high-performance OSCs.

关键词： Organic solar cells Non-fullerene acceptor Phenazine Central core End group