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SPEED:an integrated,smartphone-operated,handheld digital PCR Device for point-of-care testing

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Microsystems & Nanoengineering 2024年第3期10卷 149-158页

作者： Haoqing Zhang Xiaocheng Liu Xinlu Wang Zhiqiang yan ying Xu Martina gaňová TomášŘezníček Marie Korabečná Pavel Neuzil Ministry of Education Key Laboratory of Micro and Nano Systems for Aerospace School of Mechanical EngineeringNorthwestern Polytechnical University127 West Youyi RoadXi’anShaanxi 710072PR China The Key Laboratory of Biomedical Information Engineering of the Ministry of Education School of Life Science and TechnologyXi’an Jiaotong UniversityXi’anShaanxi 710049PR China Bioinspired Engineering and Biomechanics Center(BEBC) Xi’an Jiaotong UniversityXi’an 710049PR China School of Marine Science and Technology Northwestern Polytechnical UniversityXi’anShaanxi 710072PR China Central European Institute of Technology Brno University of TechnologyPurkyňova 12361300 BrnoCzech Republic ITD Tech S.R.O Osvoboditelu100573581 BohumínCzech Republic Institute of Biology and Medical genetics First Faculty of MedicineCharles University and General University Hospital of PragueAlbertov 412800 PragueCzech Republic

This study elaborates on the design,fabrication,and data analysis details of SPEED,a recently proposed smartphonebased digital polymerase chain reaction(dPCR)*** dPCR chips incorporate partition diameters ranging from 50μm to 5μm,and these partitions are organized into six distinct blocks to facilitate image *** to the superior thermal conductivity of Si and its potential for mass production,the dPCR chips were fabricated on a Si substrate.A temperature control system based on a high-power density Peltier element and a preheating/cooling PCR protocol user interface shortening the thermal cycle *** optical design employs four 470 nm light-emitting diodes as light sources,with filters and mirrors effectively managing the light emitted during *** algorithm is utilized for image processing and illumination nonuniformity correction including conversion to a monochromatic format,partition identification,skew correction,and the generation of an image correction *** validated the device using a range of deoxyribonucleic acid targets,demonstrating its potential applicability across multiple ***,we provide guidance and verification of the design and testing of the recently proposed SPEED device.

关键词： correction smart testing

Is It about a Bicycle? A Posthumanist Perspective on the Role of Women’s Bicycles in WWII Resistance Movements

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Sociology Study 2024年第4期14卷 198-211页

作者： Zuzana Križalkovičová Academy of Fine Arts Vienna Austria

Drawing upon Donna Haraway’s cyborg theory,this paper argues that the bicycle can be conceived as a cyborg extension of body and identity which transcended its mechanical essence to become an integral co-partner for women involved in resistance activities in *** specifically,the study explores how women used cycling during World War Ⅱ not only for mobility but as a profound expression of courage,endurance and solidarity which enabled them to overcome physical and symbolic barriers under totalitarian *** bicycle emerges not only as a central co-agent but also actively co-creates the situations in which it emerges as a transformative *** acted as a catalyst for personal and collective empowerment,fostering new subjectivities while transcending physical and social *** study sparks multidimensional discourse on how technological tools can co-create our societies and identities,offering insights into how bicycles can drive socio-political change,promote emancipation and foster inclusivity.

关键词： bicycle female involvement in World WarⅡresistance movements cyborg theory

Sc元素添加对双辊铸轧Al−Cu−Li−Mg−Zr基合金热处理的影响

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Transactions of Nonferrous Metals Society of China 2024年第6期34卷 1759-1773页

作者： Rostislav KRÁLÍK Barbora KŘIVSKÁ Lucia BAJTOŠovÁ Mykhailo STOLBCHENKO Mirko SCHAPER Olexandr gRyDIN Miroslav CIESLAR Department of Physics of Materials Faculty of Mathematics and PhysicsCharles UniversityKe Karlovu 512116PragueCzech Republic Department of Materials Science Faculty of Mechanical EngineeringUniversity of PaderbornWarburger Straße 10033098PaderbornGermany

提出制备含Sc的Al−Cu−Li−Mg−Zr基合金带材的工艺方案。即先采用双辊铸轧技术制备近净形状的3 mm厚带材,再对其进行均匀化/固溶处理。研究两种Al−Cu−Li−Mg−Zr基双辊铸轧合金,其中一种添加质量分数为0.17%的Sc,另一种未添加Sc。两种合金... 详细信息

提出制备含Sc的Al−Cu−Li−Mg−Zr基合金带材的工艺方案。即先采用双辊铸轧技术制备近净形状的3 mm厚带材,再对其进行均匀化/固溶处理。研究两种Al−Cu−Li−Mg−Zr基双辊铸轧合金,其中一种添加质量分数为0.17%的Sc,另一种未添加Sc。两种合金均先在300℃下退火30 min,然后在450℃下退火30 min以及530℃下退火30 min,随后进行室温水淬。前两段退火后组织中生成了细小、弥散的Al_(3)Zr/Al_(3)(Sc,Zr)颗粒。最后的退火步骤起到了均匀化/固溶处理的作用。双辊铸轧材料的细小晶粒结构使含有主要合金元素的原始颗粒在较短的退火时间内溶解。缩短保温时间可以限制表面层中Li的耗尽和Al_(3)Zr/Al_(3)(Sc,Zr)弥散相的明显粗化。铸态含Sc材料已经具有明显细小的晶粒结构,而经均匀化/固溶处理后生成的Al_(3)(Sc,Zr)弥散相抑制了晶粒在高温下发生粗化。在后续180℃下时效处理后,形成了θʹ(Al_(2)Cu)、T1(Al_(2)CuLi)和S'(Al_(2)CuMg)强化相。在峰值时效状态下,屈服强度提高了200 MPa,这一数值与类似直接冷却铸造材料的相当,从而证实了所提方案的合理性。

关键词：显微组织力学性能 Al−Cu−Li基合金 Sc合金化双辊铸轧

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Changes in the adult sex ratio of six duck species breeding populations over two decades

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Avian Research 2024年第2期15卷 202-210页

作者： Monika Homolkova Petr Musil Diego Pavon-Jordan Dorota gajdosova Zuzana Musilova Sarka Neuzilova Jan Zouhar Department of Ecology Faculty of Environmental SciencesCzech University of Life Sciences PragueKamýcka 129CZ-16521Prague–SuchdolCzech Republic Department of Terrestrial Ecology Norwegian Institute for Nature Research(NINA)PO Box 5685 TorgardenTrondheimN-7485Norway Department of Econometrics Faculty of Informatics and StatisticsUniversity of Economicsnam.W.Churchilla 4CZ-13067Prague 3Czech Republic

Despite all efforts,long-term changes in the adult sex ratios of breeding duck populations are still unclear;this uncertainty is especially true for male-bias populations,which are often under the scrutiny of researchers lacking convenient results for the active protection of endangered *** with male-bias populations are usually strongly affected by a decline in population size that leads to a higher extinction *** this study,we examined our long-term data of the abundance of breeding populations in six duck species(Mallard Anas platyrhynchos,gadwall Mareca strepera,Red-crested Pochard Netta rufina,Common Pochard Aythya ferina,Tufted Duck Aythya fuligula,and Common goldeneye Bucephala clangula)from fishponds in South Bohemia,Czechia,between 2004 and *** evidence was used to assess long-term changes in the adult sex ratio in these breeding populations and investigate the possible effects of the NAO index(North Atlantic Oscillation index)on them,indicating climate conditions in *** determined a long-term decrease of the proportion of females in the breeding season in two of the six examined species:Common Pochard and Red-crested Pochard,which is driven by the long-term increase in the number of males in contrast to the decreasing or stable number of females likely caused by different migration behaviours between females and *** the case of Common Pochard,in breeding populations,we estimated 60-65%of males in the early 2000s rising to 75-80%in the early ***,we establish no significant effects linked to climate conditions of the previous winter in these species as a crucial cause of the changes of the proportion of females in the breeding population.

关键词： Adult sex ratio Breeding population Population dynamics Red-listed species Waterfowl Winter weather conditions

Anisotropy effect on strengths of metamorphic rocks

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Journal of Rock Mechanics and geotechnical Engineering 2018年第1期10卷 164-175页

作者： Ahmet ?zbek Murat gül Ergun Karacan ovünc Alca Department of geological Engineering Kahramanmaras Sutcu Imam UniversityAvsar CampusKahramanmarasTurkey Department of geological Engineering Mugla Sltkl Kocman UniversityMuglaTurkey

This paper aims to study the effect of anisotropy on strengths of several metamorphic rocks of southern(Cine) submassif of Menderes metamorphic massif in southwest Turkey. Four different metamorphic rocks including foliated phyllite, schist, gneiss and marble(calcschist) were selected and *** surveys were made along lines for each rock and evaluated with DIPS program. L-type Schmidt hammer was applied in the directions parallel and perpendicular to foliation during the field study. Several hand samples and rock blocks were collected during the field study for measurements of dry and saturated densities, dry and saturated unit weights and porosity, and for petrographic analysis and strength determination in laboratory. L-and N-type Schmidt hammers were applied in the directions perpendicular(anisotropy angle of 0°) and parallel(anisotropy angle of 90) to the foliation on selected blocks of phyllite, schist, gneiss and marble(calcschist). The phyllite and schist have higher porosity and lower density values than the other rocks. However, coarse crystalline gneiss and marble(calcschist) have higher rebound values and strengths, and they are classified as strong-very strong rocks. generally, the rebound values in the direction perpendicular to the foliation are slightly higher than that in the direction parallel to foliation. Rebound values of N-type Schmidt hammer are higher than the L-type values except for phyllite. Sometimes, the rebound values of laboratory and field applications gave different results. This may result from variable local conditions such as minerals differentiation,discontinuities, water content, weathering degree and thickness of foliated structure.

关键词： Rock anisotropy Field survey Schmidt hammer rebound values Southwest Turkey

Neuroprotective agents effective against radiation damage of central nervous system

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Neural Regeneration Research 2022年第9期17卷 1885-1892页

作者： Mária Lalkovičová Laboratory of Radiation Biology Joint Institute for Nuclear ResearchDubnaRussia Slovak Academy of Sciences Institute of Experimental PhysicsKošiceSlovakia

Ionizing radiation caused by medical treatments,nuclear events or even space flights can irreversibly damage structure and function of brain *** can result in serious brain damage,with memory and behavior disorders,or even fatal oncologic or neurodegenerative *** used treatments and drugs are mostly targeting biochemical processes of cell apoptosis,radiation toxicity,neuroinflammation,and conditions such as cognitive-behavioral disturbances or others that result from the radiation *** most drugs,the side effects and potential toxicity are also to be ***,many agents have not been approved for clinical use *** this review,we focus on the latest and most effective agents that have been used in animal and also in the human research,and clinical *** could have the potential therapeutical use in cases of radiation damage of central nervous system,and also in prevention considering their radioprotecting effect of nervous tissue.

关键词： antioxidants biomedical neuroprotectants central nervous system ionizing radiation neuroprotection radiation injury radiomitigators radioprotectants radioprotection therapeutics

KRT6A介导Wnt/β-catenin信号通路调节上皮-间质转化促进非小细胞肺癌A549细胞抗辐射

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中国医科大学学报 2024年第7期53卷 628-634页

作者：尹策刘畅冯革中国医科大学附属盛京医院肿瘤中心沈阳110004 中国医科大学附属第一医院放疗科沈阳110001

目的探讨KRT6A对非小细胞肺癌A549细胞抗辐射的促进作用,并阐明其作用机制。方法诱导并建立抗辐射A549(A549-RR)细胞,通过CCK-8法、平板克隆形成实验和流式细胞术验证细胞构建成功。Western blotting检测A549和A549-RR细胞中KRT6A表... 详细信息

目的探讨KRT6A对非小细胞肺癌A549细胞抗辐射的促进作用,并阐明其作用机制。方法诱导并建立抗辐射A549(A549-RR)细胞,通过CCK-8法、平板克隆形成实验和流式细胞术验证细胞构建成功。Western blotting检测A549和A549-RR细胞中KRT6A表达情况。将A549-RR细胞分为敲减对照组(sh-NC组)和敲减KRT6A组(sh-KRT6A组),Western blotting检测KRT6A、上皮-间质转化(EMT)相关蛋白(E-cadherin、N-cadherin、Vimentin、Snail和Slug)以及β-catenin的表达;采用CCK-8法、平板克隆形成实验和流式细胞术检测细胞增殖及凋亡情况。将A549-RR细胞分为sh-NC组、sh-KRT6A组、敲减KRT6A和过表达对照(shKRT6A+ov-NC组)以及敲减KRT6A和过表达β-catenin (sh-KRT6A+ov-β-catenin组),Western blotting检测β-catenin以及EMT相关蛋白E-cadherin、N-cadherin、Vimentin、Snail和Slug的表达;采用CCK-8法、平板克隆形成实验和流式细胞术检测细胞增殖及凋亡情况。结果成功建立了A549-RR细胞,A549-RR细胞中KRT6A表达上调。敲减KRT6A降低A549-RR细胞增殖活性和克隆形成能力,增加细胞凋亡率,上调E-cadherin蛋白表达并下调N-cadherin、Vimentin、Snail、Slug和β-catenin蛋白表达;过表达β-catenin可逆转此作用。结论 KRT6A在A549-RR细胞中表达上调,敲减KRT6A可降低A549-RR细胞的抗辐射能力,其机制可能与激活Wnt/β-catenin信号通路诱导的EMT有关。

关键词：非小细胞肺癌角蛋白6A 放疗上皮-间质转化 Wnt/β-catenin信号通路

Combination therapy with oncolytic virus and T cells or mRNA vaccine amplifies antitumor effects

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Signal Transduction and Targeted Therapy 2024年第6期9卷 2655-2668页

作者： Rao Fu Ruoyao Qi Hualong Xiong Xing Lei yao Jiang Jinhang He Feng Chen Liang Zhang Dekui Qiu yiyi Chen Meifeng Nie Xueran guo yuhe Zhu Jinlei Zhang Mingxi yue Jiali Cao guosong Wang yuqiong Que Mujing Fang yingbin Wang yixin Chen Tong Cheng Shengxiang ge Jun Zhang Quan yuan Tianying Zhang Ningshao Xia State Key Laboratory of Vaccines for Infectious Diseases Xiang An Biomedicine LaboratoryDepartment of Laboratory MedicineSchool of Public Health&School of Life SciencesXiamen UniversityXiamen 361102 FujianChina National Institute of Diagnostics and Vaccine Development in Infectious Diseases Xiamen 361102 FujianChina

Antitumor therapies based on adoptively transferred T cells or oncolytic viruses have made significant progress in recent years,but the limited efficiency of their infiltration into solid tumors makes it difficult to achieve desired antitumor effects when used *** this study,an oncolytic virus(rVSV-LCMVg)that is not prone to induce virus-neutralizing antibodies was designed and combined with adoptively transferred T *** transforming the immunosuppressive tumor microenvironment into an immunosensitive one,in B16 tumor-bearing mice,combination therapy showed superior antitumor effects than *** occurred whether the ov was administered intratumorally or *** therapy significantly increased cytokine and chemokine levels within tumors and recruited CD8^(+)T cells to the TME to trigger antitumor immune *** with adoptively transferred T cells and subsequent oncolytic virotherapy sensitizes refractory tumors by boosting T-cell recruitment,downregulating the expression of PD-1,and restoring effector T-cell *** offer a combination therapy with greater translational value,mRNA vaccines were introduced to induce tumor-specific T cells instead of adoptively transferred T *** combination of ovs and mRNA vaccine also displays a significant reduction in tumor burden and prolonged *** study proposed a rational combination therapy of ovs with adoptive T-cell transfer or mRNA vaccines encoding tumor-associated antigens,in terms of synergistic efficacy and mechanism.

关键词： transferred rational boosting

Predicting the output error of the suboptimal state estimator to improve the performance of the MPC-based artificial pancreas

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Control Theory and Technology 2023年第4期21卷 541-554页

作者： Martin Dodek Eva Miklovičová Institute of Robotics and Cybernetics Faculty of Electrical Engineering and Information TechnologySlovak University of Technology in BratislavaIlkovičova 3Bratislava81219Slovakia

The error of single step-ahead output prediction is the information traditionally used to correct the state estimate while exploiting the new measurement of the system ***,its dynamics and statistical properties can be further studied and exploited in other *** is known that in the case of suboptimal state estimation,this output prediction error forms a correlated sequence,hence it can be effectively predicted in real *** a suboptimal scenario is typical in applications where the process noise model is not known or it is ***,the paper deals with the problems of analytical and empirical modeling,identification,and prediction of the output error of the suboptimal state estimator for the sake of improving the output prediction accuracy and ultimately the performance of the model predictive *** improvements are validated on an empirical model of type 1 diabetes within an in-silico experiment focused on glycemia prediction and implementation of the MPC-based artificial pancreas.

关键词： Suboptimal state estimation Prediction Autoregressive model Moving average model Model predictive control Diabetes mellitus Artificial pancreas