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心肌缺血再灌注损伤中心肌免疫细胞单细胞图谱绘制及具有心肌保护效应的Ym-1hi中性粒细胞的鉴定

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Science Bulletin 2024年第7期69卷 949-967页

作者：董亚兰康真玉张子力张永强周海锋刘艳飞帅欣欣李君仪殷梁青青王埙埙马彦范恒 Feng Jiang 林志豪丁从珠金渊真 Alexey Sarapultsev 李芳菲张戈谢天尹长军程翔罗珊珊刘玥胡德胜 Department of Integrated Traditional Chinese and Western Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China hubei Key Laboratory of Biological Targeted Therapy Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Nanjing Drum Tower hospital Clinical College of Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjing 210008China National Clinical Research Center for Chinese Medicine Cardiology Xiyuan HospitalChinese Academy of Chinese Medical SciencesBeijing 100091China Department of Cardiology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of International Education Tianjin University of Traditional Chinese MedicineTianjin 300193China Institute of Neuroscience School of MedicineXiamen UniversityXiamen 361102China School of Traditional Chinese Medicine Xiamen University MalaysiaSepang 43900Malaysia School of Medical Biology South Ural State UniversityChelyabinsk 620049Russia Shum Yiu Foon Sum Bik Chuen Memorial Centre for Cancer and Inflammation Research(CCIR) School of Chinese MedicineHong Kong Baptist UniversityHong Kong 999077China Institute of Integrated Bioinfomedicine and Translational Science School of Chinese MedicineHong Kong Baptist UniversityHong Kong 999077China Division of Vascular Surgery The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China Institute of Precision Medicine The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China Institute for Cardiovascular Prevention(IPEK) Ludwig-Maximilians-UniversityMunich 80336Germany Institute of hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China China-Russia Medical Research Center for Stress Immunology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Myocardial ischemia–reperfusion injury(MIRI)is a major hindrance to the success of cardiac reperfusion *** increased neutrophil infiltration is a hallmark of MIRI,the subtypes and alterations of neutrophils in this process remain ***,we performed single-cell sequencing of cardiac CD45^(+)cells isolated from the murine myocardium subjected to MIRI at six-time *** identified diverse types of infiltrating immune cells and their dynamic changes during *** neutrophils showed the most immediate response and largest changes and featured with functionally heterogeneous subpopulations,including Ccl3^(hi)Neu and Ym-1^(hi)Neu,which were increased at 6 h and 1 d after reperfusion,***-1^(hi)Neu selectively expressed genes with protective effects and was,therefore,identified as a novel specific type of cardiac cell in the injured *** analysis indicated that neutrophils and their subtypes orchestrated subsequent immune responses in the cardiac tissues,especially instructing the response of *** abundance of Ym-1^(hi)Neu was closely correlated with the therapeutic efficacy of MIRI when neutrophils were specifically targeted by anti-Lymphocyte antigen 6 complex locus g6D(Ly6g)or anti-Intercellular cell adhesion molecule-1(ICAM-1)neutralizing *** addition,a neutrophil subtype with the same phenotype as Ym-1^(hi)Neu was detected in clinical samples and correlated with ***-1 inhibition exacerbated myocardial injury,whereas Ym-1 supplementation significantly ameliorated injury in MIRI mice,which was attributed to the tilt of Ym-1 on the polarization of macrophages toward the repair phenotype in myocardial ***,our findings reveal the antiinflammatory phenotype of Ym-1^(hi)Neu and highlight its critical role in myocardial protection during the early stages of MIRI.

关键词： Myocardial ischemia-reperfusion injury Neutrophil Ym-1 Cardiac immune response Single-cell sequencing

转基因小鼠中人清道夫受体AI基因的稳定遗传和特异表达

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中国动脉硬化杂志 2000年第1期8卷 5-12页

作者：万腊香杨永宗 SookjaS.Chung StephenS.M.Chung 曹德良吴孟津万载阳陈修衡阳医学院分子生物学研究中心香港大学分子生物学研究所 Department of Pharmacology Yale University School of MedicineNew HavenConnecticut06520-8066USA 湖南医科大学药理学教研室

为阐明人清道夫受体AI (hSR -AI)的功能及在动脉粥样硬化发生中的作用 ,本研究首先构建了含鼠tie- 1启动子和人清道夫受体AIcDNA的表达载体 ,经酶切及测序鉴定后 ,用显微注射的方法将制备好的tie - 1-hSR-A -BghpolyA片段注入受精卵 ,... 详细信息

为阐明人清道夫受体AI (hSR -AI)的功能及在动脉粥样硬化发生中的作用 ,本研究首先构建了含鼠tie- 1启动子和人清道夫受体AIcDNA的表达载体 ,经酶切及测序鉴定后 ,用显微注射的方法将制备好的tie - 1-hSR-A -BghpolyA片段注入受精卵 ,将注射后存活的受精卵移入ICR假孕母鼠 ,产下的仔鼠经聚合酶链反应和Southernblot分析 ,筛选出整合有外源目的基因的阳性转基因鼠 ;对小鼠组织RNA行逆转录聚合酶链反应及组织切片免疫组织化学染色 ,检测人清道夫受体AI在小鼠体内的表达水平及表达部位 ;光镜及电镜观察转基因鼠血管及其它组织的病理变化。结果发现 ,重组Tie - 1/hSR -AI质粒中鼠tie - 1启动子和人清道夫受体AIcDNA序列正确 ,显微注射后存活的 5 6 1枚受精卵分别移入 19只ICR假孕母鼠 ,有 13只受孕 ,共产下 5 6只仔鼠 ,存活 5 4只 ,经过整合检测 ,检出 7只阳性鼠 ,整合效率为 13%。在g1、g2、g3代及纯合子转基因鼠中PCR阳性率分别为 47.8%、71.3%、75 .0 %和 10 0 %。 5只雄性转基因鼠的主动脉、肾、肝等组织中均有人清道夫受体AI表达 ,且主要集中在血管的内皮细胞上 ;转基因鼠主动脉内皮细胞明显水肿 ,表面呈多囊状和虫蚀样改变 ,胞质中有较多水泡 ,中膜有糖原沉积样灶性病变 ,平滑肌细胞中亦有水肿 ;血浆?

关键词：人清道夫受体AI 转基因动脉粥样硬化遗传

JAK/STAT3抑制剂对创伤性休克致兔肾损伤影响

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创伤与急危重病医学 2017年第1期5卷 18-21页

作者：刘学磊柳云恩佟周佟昌慈张玉彪施琳丛培芳史秀云刘颖金红旭侯明晓沈阳军区总医院急诊医学部辽宁沈阳110016 沈阳军区总医院全军重症(战)创伤救治中心实验室及辽宁省重症创伤和器官保护重点实验室辽宁沈阳110016

目的探讨JAK/STAT3抑制剂Ag490对创伤性休克致兔肾损伤的影响。方法将30只健康新西兰兔分为对照组、模型组与Ag490治疗组,每组10只。模型组与Ag490治疗组兔建立创伤性休克致肾损伤动物模型。通过实时荧光定量聚合酶链反应(Real-time PCR... 详细信息

目的探讨JAK/STAT3抑制剂Ag490对创伤性休克致兔肾损伤的影响。方法将30只健康新西兰兔分为对照组、模型组与Ag490治疗组,每组10只。模型组与Ag490治疗组兔建立创伤性休克致肾损伤动物模型。通过实时荧光定量聚合酶链反应(Real-time PCR)和蛋白质印迹法(Western-blot)检测肾组织中STAT3、kim-1、TgF-β1的表达情况。结果与对照组比较,模型组肾组织肌酐与尿素氮水平显著高于对照组,差异有统计学意义(Pg490治疗组肌酐与尿素氮水平显著降低,差异有统计学意义(PgF-β1的mRNA与蛋白表达水平显著升高,差异有统计学意义(Pg490治疗组STAT3、TgF-β1的mRNA与蛋白表达水平显著降低,差异有统计学意义(PgF-β1来阻断JAK/STAT3信号通路的激活,进而抑制创伤性休克肾损伤发展。

关键词：创伤性休克肾损伤 JAK/STAT3信号通路 TgF-β1 Ag490

Nature:科学家鉴别出调节机体睡眠的两个关键基因

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现代生物医学进展 2017年第6期17卷 I0002-I0002页

作者： hiromasa Funato, Chika Miyoshi, Tomoyuki Fujiyama, Takeshi Kanda, Makito Sato, Zhiqiang Wang, Jing Ma, Aya Ikkyu, Miyo Kakizaki, Noriko hotta-hirashima, Satomi Kanno, haruna Komiya, Fuyuki Asano, Takato honda, Staci J. kim, Kanako harano, hiroki Muramoto, Toshiya Yonezawa, Shinichi Miyazaki, Linzi Connor, Yu hayashi, Qinghua Liu, Joseph S. Takahashi Masashi Yanagisawa hiromasa Funato Makito Sato Masashi Yanagisawa Shin Nakane, Jun Tomita Kazuhiko Kume Seiya Mizuno, Fumihiro Sugiyama Satoru Takahashi Vivek Kumar Joseph S. Takahashi Vivek Kumar Ikuo Miura, Tomohiro Suzuki Shigeharu Wakana Atsushi Watanabe Manabu Abe Kenji Sakimura Yu hayashi Qinghua Liu Joseph S. Takahashi Masashi Yanagisawa Masashi Yanagisawa International Institute for Integrative Sleep Medicine (WPI-IIIS) University of Tsukuba Tsukuba Ibaraki 305-8575 Japan Department of Anatomy Faculty of Medicine Toho University Ota-ku Tokyo 143-8540 Japan Department of Molecular genetics University of Texas Southwestern Medical Center Dallas Texas 75390 USA Department of Neuropharmacology Graduate School of Pharmaceutical Sciences Nagoya City University Nagoya Aichi 467-8603 Japan Laboratory Animal Resource Center University of Tsukuba Tsukuba Ibaraki 305-8575 Japan Department of Neuroscience University of Texas Southwestern Medical Center Dallas Texas 75390 USA The Jackson Laboratory Bar Harbor Maine 04609 USA Technology and Development Team for Mouse Phenotype Analysis RIKEN Bioresource Center Tsukuba Ibaraki 305-0074 Japan Laboratory of Research Advancement National Center for Geriatrics and Gerontology Obu Aichi 474-8511 Japan Department of Cellular Neurobiology Brain Research Institute Niigata University Niigata 951-8585 Japan PRESTO Japan Science and Technology Agency Kawaguchi Saitama 332-0012 Japan Department of Biochemistry University of Texas Southwestern Medical Center Dallas Texas 75390 USA howard hughes Medical Institute University of Texas Southwestern Medical Center Dallas Texas 75390 USA Life Science Center Tsukuba Advanced Research Alliance University of Tsukuba Tsukuba Ibaraki 305-8575 Japan

近日，刊登在国际杂志Nature上的一项研究报告中，来自西南医学中心等机构的研究人员通过研究鉴别出了两个关键的核心基因，这两个基因能够帮助调节机体深度睡眠和做梦的水平，相关研究或为阐明相关的基因控制睡眠的网络提供新的线索。... 详细信息

近日，刊登在国际杂志Nature上的一项研究报告中，来自西南医学中心等机构的研究人员通过研究鉴别出了两个关键的核心基因，这两个基因能够帮助调节机体深度睡眠和做梦的水平，相关研究或为阐明相关的基因控制睡眠的网络提供新的线索。此前研究者通过对小鼠进行研究发现了能够控制机体快速眼动睡眠的基因，同时该基因还能够诱导深度睡眠。而第二个基因能够控制机体对快速眼动睡眠的需求量，相关研究为科学家们提供了一个关键的切入点来帮助解释睡眠工作的机制，同时研究者还鉴别出了治疗睡眠障碍的潜在靶点。

关键词：睡眠障碍核心基因科学家机体鉴别基因控制研究人员需求量

CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed Xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves Weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai Yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay Shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci Xiao-Jun Bi Yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev giacomo Cacciapaglia hua Cai Wenyong Cai Wujun Cai Yijian Cai Yizhou Cai Yuchen Cai haiying Cai huacheng Cai Lorenzo Calibbi Junsong Cang guofu Cao Jianshe Cao Antoine Chance Xuejun Chang Yue Chang Zhe Chang Xinyuan Chang Wei Chao Auttakit Chatrabhuti Yimin Che Yuzhi Che Bin Chen Danping Chen Fuqing Chen Fusan Chen gang Chen guoming Chen hua-Xing Chen huirun Chen Jinhui Chen Ji-Yuan Chen Kai Chen Mali Chen Mingjun Chen Mingshui Chen Ning Chen Shanhong Chen Shanzhen Chen Shao-Long Chen Shaomin Chen Shiqiang Chen Tianlu Chen Wei Chen Xiang Chen Xiaoyu Chen Xin Chen Xun Chen Xurong Chen Ye Chen Ying Chen Yukai Chen Zelin Chen Zilin Chen gang Chen Boping Chen Chunhui Chen hok Chuen Cheng huajie Cheng Shan Cheng Tongguang Cheng Yunlong Chi Pietro Chimenti Wen han Chiu guk Cho Ming-Chung Chu Xiaotong Chu Ziliang Chu guglielmo Coloretti Andreas Crivellin hanhua Cui Xiaohao Cui Zhaoyuan Cui Brunella D'Anzi Ling-Yun Dai Xinchen Dai Xuwen Dai Antonio De Maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De Sio Elisa Del Core Shuangxue Deng Wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco Ran Ding Siqin Dingl Yadong Ding haiyi Dong Jianin Agh University of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui University HefeiAnhui Applied Physics Department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras hindu University Institute of ScienceVaranasi Bandirma Onyedi Eylul University Bandirma Beihang University Beijing Beijing Computational Science Research Center Beijing Beijing Conveyi LTD Beijing Beijing glass Research Institute Co. LtdBeijing Beijing Institute of Technology Beijing Beijing Normal University Beijing Brown University ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad University Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo University Giza Center for Theory and Computation National Tsing Hua UniversityHsinchu Central China Normal University WuhanHubei Central South University ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa Chengdu KaitengSifang Digital Radio&TV Equipment Co. LtdChengduSichuan China academy of engineering physics MianyangSichuan China building materials academy Beijing China Center of Advanced Science and Technology Beijing China Institute of Atomic Energy Beijing China Jiliang University HangzhouZhejiang China Nuclear Instrument CO. LTDBeijing China University of geosciences Beijing China University of geosciences WuhanHubei China University of Mining and Technology XuzhouJiangsu China West Normal University NanchongSichuan Chongqing University Department of PhysicsChongqing Chulalongkorn University Bangkok City University of hong Kong Department of PhysicsHong Kong Cockcroft Institute Daresbury Cornell University IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu University DalianLiaoning Dalian University of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP JINR) Dubna East China University Of Science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 geV Linac,a 1.1 geV Damping Ring,a Booster capable of achieving energies up to 180 geV,and a Collider operating at varying energy modes(Z,W,h,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million higgs bosons,facilitating precise measurements of higgs coupling at sub-percent levels,exceeding the precision expected from the hL-LhC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise