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Assessment of probiotic and antifungal activity of Lactiplantibacillus plantarum MYSAgT3 isolated from locally available herbal juice against mycotoxigenic Aspergillus species

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Food Bioscience 2022年第6期50卷 1549-1560页

作者： g.adithi Rakesh Somashekaraiah S.Divyashree B.Shruthi M.Y.Sreenivasa Applied Mycology Lab Department of Studies in MicrobiologyUniversity of MysoreManasagangotriMysuruIndia

This study investigated the antifungal activities of probiotic bacteria isolated from traditional herbal juice, which was a blend of Tulsi, Mint, Bael and Indian borage leaves. About 20 isolates were obtained from the herbal juice sample;these isolates were initially screened against fungal pathogens such as Aspergillus parasiticus , Aspergillus flavus , Aspergillus ochraceus , Aspergillus carbonarius and Aspergillus fumigatus . Of the 20 isolates obtained, MYSAgT3 and MYSAgT4 exhibited potential antifungal activity against the tested fungal pathogens. Moreover, MYSAgT3 and MYSAgT4 was found to survive acidic pH and bile conditions, but were susceptible to tested antibiotics. Both the isolates exhibited 40–60% of autoaggregation ability, >90% of cell surface hydrophobicity and tolerance to 0.4–0.6% phenol. The 16S rRNA sequencing results identified the isolates MYSAgT3 and MYSAgT4 as Lactiplantibacillus plantarum. The MYSAgT3 exhibited potent antagonistic and probiotic activity compared to MYSAgT4;hence MYSAgT3 was further used for the control of Aspergillus ochraceus. Initial antifungal activity of MYSAgT3 inhibited about 76.67% growth of Aspergillus ochraceus comparatively higher than other Aspergillus sp. tested. Co-inoculation and microdilution assay revealed the high inhibitory activity of Lactiplantibacillus plantarum MYSAgT3. Further, cell free supernatant (CFS) of Lactiplantibacillus plantarum MYSAgT3 reduced the growth of fungal hyphae and conidial germination of Aspergillus ochraceus in conidial germination assay and bio preservative activity on peanuts. The CFS characterization and Liquid chromatography-mass spectroscopy (LCMS) results showed that, the antifungal activity was due to different organic acids present in the CFS of MYSAgT3. MYSAgT3 and its CFS also reduced the growth of Aspergillus ochraceus in Poultry feeds and about 21.80% of ochratoxin A (OTA) degradation was attained after 24 h of incubation with MYSAgT3. The isolated Lactic acid bacteria

关键词： Aspergillus ochraceus Lactiplantibacillus plantarum Cell free supernatant Antifungal activity Ochratoxin A

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An SPH Framework for Earthquake-Induced Liquefaction Hazard Assessment of geotechnical Structures

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 251-277页

作者： Sourabh Mhaski g.V.Ramana Department of Civil Engineering Indian Institute of Technology DelhiNew Delhi110016India

Earthquake-induced soil liquefaction poses significant risks to the stability of geotechnical structures *** understanding of the liquefaction triggering,and the post-failure large deformation behaviour is essential for designing resilient *** present study develops a Smoothed Particle Hydrodynamics(SPH)framework for earthquake-induced liquefaction hazard assessment of geotechnical *** coupled flowdeformation behaviour of soils subjected to cyclic loading is described using the PM4Sand model implemented in a three-phase,single-layer SPH framework.A staggered discretisation scheme based on the stress particle SPH approach is adopted to minimise numerical inaccuracies caused by zero-energy modes and tensile ***,non-reflecting boundary conditions for seismic analysis of semi-infinite soil domains using the SPH method are *** numerical framework is employed for the analysis of cyclic direct simple shear test,seismic analysis of a level ground site,and liquefaction-induced failure of the Lower San Fernando *** agreement for liquefaction triggering and post-failure behaviour demonstrates that the SPH framework can be utilised to assess the effect of seismic loading on field-scale geotechnical *** present study also serves as the basis for future advancements of the SPH method for applications related to earthquake geotechnical engineering.

关键词： Earthquake seismic liquefaction stress particle PM4Sand smoothed particle hydrodynamics(SPH)

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Synthetic biology techniques to tackle heavy metal pollution and poisoning

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Synthetic and Systems Biotechnology 2022年第3期7卷 841-846页

作者： adithi Somayaji Soumodeep Sarkar Shravan Balasubramaniam Ritu Raval Department of Biotechnology Manipal Institute of TechnologyManipal Academy of Higher EducationManipalIndia Manipal BioMachines Manipal Institute of TechnologyManipal Academy of Higher EducationManipalIndia

The requirement for natural resources and energy increases continually with the increase in *** inevitable result of this is soil,water,and air pollution with diverse pollutants,including heavy *** Biology involves using modular,interchangeable biological parts,devices in standard chassis or whole organisms to achieve a programmed result that can be quantified and optimized till it meets the required *** makes synthetic biology techniques very popular to tackle pressing global issues such as heavy metal *** review aimed to highlight various advancements as well as benefits,risks,and problems in synthetic biology techniques for detection,bioaccumulation,and biosorption of various heavy metals using engineered *** found that while such an approach is cost-effective,accessible,and efficient,there are several inherent technological and ethical issues including but not limited to metabolic burden and consequences of use of genetically modified organisms *** these hurdles will probably take time and innumerable conversations,and should be done through education and a culture of responsible research,rather than enforcing restrictions on the development of synthetic biology.

关键词： Heavy metals Biosensors Bioremediation Synthetic biology Responsible research

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Stepping up after spinal cord injury:negotiating an obstacle during walking

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Neural Regeneration Research 2025年第7期20卷 1919-1929页

作者： Alain Frigon Charly g.Lecomte Department of Pharmacology-Physiology Faculty of Medicine and Health SciencesUniversitéde SherbrookeCentre de recherche du Centre Hospitalier Universitaire de SherbrookeSherbrookeQCCanada

Every day walking consists of frequent voluntary modifications in the gait pattern to negotiate *** spinal cord injury,stepping over an obstacle becomes *** over an obstacle requires sensorimotor transformations in several structures of the brain,including the parietal cortex,premotor cortex,and motor *** information and planning are transformed into motor commands,which are sent from the motor cortex to spinal neuronal circuits to alter limb trajectory,coordinate the limbs,and maintain *** spinal cord injury,bidirectional communication between the brain and spinal cord is disrupted and animals,including humans,fail to voluntarily modify limb trajectory to step over an ***,in this review,we discuss the neuromechanical control of stepping over an obstacle,why it fails after spinal cord injury,and how it recovers to a certain extent.

关键词： biomechanics locomotion neurophysiology obstacle negotiation spinal cord injury

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Bioelectronic medicine in modulation of cortical spreading depolarization and beyond

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Neural Regeneration Research 2025年第2期20卷 481-482页

作者： Khaled Alok Timothy g.White Chunyan Li Department of Neurosurgery Donald and Barbara Zucker School of Medicine at Hofstra/NorthwellHempsteadNYUSA Translational Brain Research Laboratory The Feinstein Institutes for Medical ResearchManhassetNYUSA

Bioelectronic interventions,specifically trigeminal nerve st imulat ion(TNS),have attracted considerable attention in conditions where cortical spreading depolarizations(CSDs)accompanied by compromised cerebral perfusion may exacerbate neurological *** pharmacological interventions have demonstrated initial potential in addressing CSDs,a standardized treatment approach has not yet been *** objective of this perspective is to explore emerging bioelectronic methodologies for addressing CSDs,particularly emphasizing TNS,and to underscore TNS’s capacity to enhance neurovascular coupling and cerebral perfusion.

关键词： cerebral perfusion electronic

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Impact of Zika virus non-structural protein mutations on hippocampal damage

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Neural Regeneration Research 2025年第8期20卷 2307-2308页

作者： Larissa M.g.Cassiano Roney S.Coimbra Neurogenômica/Imunopatologia Instituto RenéRachouFiocruzBelo HorizonteBrazil Departamento de Bioquímica e Imunologia Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil

The Zika virus(ZIKV),a member of the Flaviviridae family,attracted worldwide attention for its connection to severe neurological effects,notably microcephaly in newborns,first reported during the 2015 epidemic in ***,its impact goes beyond fetal and neonatal abnormalities,also affecting the central nervous system(CNS)in both children and adults,leading to enduring cognitive and behavioral impairments.

关键词： damage fetal neonatal

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An experimental investigation on pulse transit time and pulse arrival time using ecg, pressure and ppg sensors

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Medicine in Novel Technology and Devices 2023年第1期17卷 116-122页

作者： Sukesh Rao M. Narendra K.C adithi K. Sanith C.Bangera Department of Electronics and Communication Engineering NMAM Institute of TechnologyNitteNitte Deemed to be UniversityNitte-574110KarnatakaIndia Department of general Medicine K.S.Hegde Medical AcademyNitte Deemed to be UniversityMangaloreKarnatakaIndia

Pulse transit time(PTT)and pulse arrival time(PAT)are explored using multiple sensors connected to the human *** wave velocity(PWV)is indirectly computed using *** conducted a study using ECg,Pressure,and PPg sensor to capture the PAT and PTT information *** PAT and three PTT values were obtained from the *** electrocardiograph(ECg/EKg)is used as a reference signal for three PAT *** values were obtained using pressure and PPg sensors connected at the wrist and fingertip respectively.A pressure sensor module was constructed to measure PTT at the wrist and the results were compared with the other standard reference *** rate variability was found to be relatively lower in both ECg and PPg sensors as compared with pressure sensors at the *** PAT obtained from ECg and PPg sensor was found to have the least variation of 0:386±0:037 sec with 9.58% of the *** at the wrist captured using a pressure sensor was recorded to be 0:026±0:013 sec with 50% of the *** values measured using ECg and pressure sensor were found to be correlating with ECg and *** experimental result indicates different ranges of the PWV which indicates that the flow velocity of the blood varies with different *** can be effectively computed from pressure and PPg sensors,and this makes it possible to extract different pulse features in the wearable devices without the need of ECg signal or PAT as reference.

关键词： Pressure Sensor Pulse Artery Wrist Signal

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Present status and future directions:Apexification

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World Journal of Methodology 2025年第1期15卷 26-32页

作者： Sachin Chauhan Radha Chauhan Prashant Bhasin Bhavna g Sharaf Department of Conservative Dentistry and Endodontics Sudha Rustagi College of Dental Sciences and ResearchFaridabad 121002HaryanaIndia Department of Prosthodontics and Crown and Bridge and Oral Implantology Mahatma Gandhi Dental College&HospitalJaipur 302022RajasthanIndia Department of Pedodontics and Preventive Dentistry Sudha Rustagi College of Dental Sciences and ResearchFaridabad 121002HaryanaIndia

The success rate of apexification is primarily determined by multiple factors,including the material used,the size of the open apex compared to the length of the root,and the technique used in each *** main objective of this review was to provide an update on the present management of open apex to identify factors and circumstances that may influence the success of apexification using different materials and *** research on apexification should focus on how to treat open apices with wide periapical lesions without ***,the predictability of these parameters with non-surgical procedures was uncertain,but now,with the use of a dental operating microscope,it has become more *** reason could be that extra visits are no longer required due to major advances in the armamentarium and materials used for apexification.

关键词： Bioceramics Magnification Biodentine Mineral trioxide aggregate Apexification Open apex

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Advancement in utilization of magnetic resonance imaging and biomarkers in the understanding of schizophrenia

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World Journal of Clinical Cases 2025年第1期13卷 11-15页

作者： Aidan K Tirpack Danyaal g Buttar Mandeep Kaur Department of Psychiatry Campbell University School of Osteopathic MedicineBuies CreekNC 27506United States Department of Psychiatry and Behavioral Health Mercyhealth Hospital and Trauma CenterJanesvilleWI 53548United States

Historically,psychiatric diagnoses have been made based on patient’s reported symptoms applying the criteria from diagnostic and statistical manual of mental *** utilization of neuroimaging or biomarkers to make the diagnosis and manage psychiatric disorders remains a distant *** have been several studies that examine brain imaging in psychiatric disorders,but more work is needed to elucidate the complexities of the human *** this editorial,we examine two articles by Xu et al and Stoyanov et al,that show developments in the direction of using neuroimaging to examine the brains of people with schizo-phrenia and *** et al used magnetic resonance imaging to examine the brain structure of patients with schizophrenia,in addition to examining neurotransmitter levels as *** et al used functional magnetic resonance imaging to look at modulation of different neural circuits by diagnostic-specific scales in patients with schizophrenia and *** two studies provide crucial evidence in advancing our understanding of the brain in prevalent psychiatric disorders.

关键词： Schizophrenia Magnetic resonance imaging Biomarkers Neurotransmitters Psychiatric disorders

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green ionic metal-organic frameworks as nanocatalysts for CO_(2)fixation

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Nano Research 2025年第1期18卷 260-269页

作者： Nuria Martín Miguel Maireles-Porcar Julián Sánchez Velandia Belén Altava Francisco g.Cirujano Eduardo garcía-Verdugo Departamento de Química Inorgánica y Orgánica Universidad Jaume IAv.Sos Baynats/nCastellóde la PlanaCastellón 12071Spain

Co,Ni,and Zn iso-reticular coordination polymers of 2,5-dihydroxyterphthalic acid(H_(2)dot)can be rapidly obtained under room temperature and aqueous conditions using either sodium or ammonium hydroxide as the bases to deprotonate the *** use of metal bromides and tetrabutylammonium hydroxide(TBAOH)as the base allows the green synthesis of ionic metal-organic frameworks(MOFs),characterized by X-ray diffraction(XRD),Fourier transform infrared(FTIR),scanning electron microscopy(SEM),thermo gravimetric analysis(TgA),H-nuclear magnetic resonance(NMR),elemental analysis,and X-ray photoelectron spectroscopy(XPS),and employed in the pressure,solvent,and additive-free CO_(2)fixation to epoxides by its cycloaddition to epichlorohydrin,exhibiting a cooperative behavior between the MOF and the ionic liquid formed within the framework,achieving TONs of the confined tetrabutylammonium bromide(TBABr)up to 700.

关键词： metal-organic frameworks(MOFs) ionic liquids heterogeneous catalysis CO_(2)conversion

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