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gRK2 mediated degradation of sav1 initiates hyperplasia of fibroblast-like synoviocytes in rheumatoid arthritis

Acta Pharmaceutica sinica B 2024年第3期14卷 1222-1240页

作者： Paipai guo Ji Jiang Rui Chu Feng he Mingli ge Ruhong Fang Qiuyun guan Huijuan Cheng Chunru Jiang Tiantian su Zhenduo Zhu Hao Liu Wei Wei shihao Zhang Qingtong Wang Institute of Clinical Pharmacology Anhui Medical UniversityKey Laboratory of Anti-inflammatory and Immune MedicineMinistry of EducationCollaborative Innovation Center of Anti-inflammatory and Immune MedicinesHefei 230032China school of Pharmacy Bengbu Medical CollegeBengbu 233030China The Third Affiliated Hospital of Anhui Medical University(the First People’s Hospital of hefei) Hefei 230061China

Hyperplasia and migration of fibroblast-like synoviocytes(FLss)are the key drivers in the pathogenesis of rheumatoid arthritis(RA)and joint *** Yes-associated protein(YAP),which is a powerful transcription co-activator for proliferative genes,was observed in the nucleus of inflammatory FLss with unknown upstream *** gene Expression Omnibus database analysis,it was found that salvador homolog-1(sav1),the pivotal negative regulator of the Hippo-YAP pathway,was slightly downregulated in RA ***,sav1 protein expression is extremely ***,it was revealed that sav1 is phosphorylated,ubiquitinated,and degraded by interacting with an important serine-threonine kinase,g protein-coupled receptor(gPCR)kinase 2(gRK2),which was predominately upregulated by gPCR activation induced by ligands such as prostaglandin E2(PgE2)in *** process further contributes to the decreased phosphorylation,nuclear translocation,and transcriptional potency of YAP,and leads to aberrant FLss *** depletion of gRK2 or inhibition of gRK2 by paroxetine rescued sav1 expression and restored YAP phosphorylation and finally inhibited RA FLss proliferation and ***,paroxetine treatment effectively reduced the abnormal proliferation of FLss in a rat model of collagen-induced arthritis which was accompanied by a significant improvement in clinical ***,these results elucidate the significance of gRK2 regulation of Hippo-YAP signaling in FLss proliferation and migration and the potential application of gRK2 inhibition in the treatment of FLss-driven joint destruction in RA.

关键词： Rheumatoid arthritis Fibroblast-like synoviocytes g protein-coupled receptor kinase 2 salvador homolog-1 Yes-associated protein

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Design of Clustering Techniques in Cognitive Radio sensor Networks

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Computer systems science & Engineering 2023年第1期44卷 441-456页

作者： R.ganesh Babu D.hemanand V.Amudha s.sugumaran Department of Electronics and Communication Engineering SRM TRP Engineering College621105Tamil NaduIndia Department of Computer science and Engineering Sriram Engineering College602024Tamil NaduIndia Department of Electronics and Communication Engineering Saveetha School of Engineering602105Tamil NaduIndia Department of Electronics and Communication Engineering Vishnu Institute of TechnologyBhimavaram534202Andhra PradeshIndia

In recent decades,several optimization algorithms have been developed for selecting the most energy efficient clusters in order to save power during trans-mission to a shorter distance while restricting the Primary Users(PUs)*** Cognitive Radio(CR)system is based on the Adaptive swarm Distributed Intelligent based Clustering algorithm(AsDIC)that shows better spectrum sensing among group of multiusers in terms of sensing error,power sav-ing,and convergence *** this research paper,the proposed AsDIC algorithm develops better energy efficient distributed cluster based sensing with the optimal number of clusters on their *** this research,multiple random sec-ondary Users(sUs),and PUs are considered for ***,the pro-posed AsDIC algorithm improved the convergence speed by combining the multi-users clustered communication compared to the existing optimization *** results showed that the proposed AsDIC algorithm reduced the node power of 9.646%compared to the existing ***,AsDIC algorithm reduced 24.23%of sUs average node power compared to the existing *** of detection is higher by reducing the signal-to-Noise Ratio(sNR)to 2 dB *** proposed AsDIC delivers low false alarm rate compared to other existing optimization algorithms in the primary *** results showed that the proposed AsDIC algorithm effectively solves the multimodal optimization problems and maximizes the performance of net-work capacity.

关键词： Adaptive swarm distributed clustering cognitive radio clustering algorithm distributed swarm intelligent energy efficient distributed cluster-based sensing multi modal optimization

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Oxidative stress indicators in human and bottlenose dolphin leukocytes in response to a pro-inflammatory challenge

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BIOCELL 2021年第6期45卷 1621-1630页

作者： TARYN E.sYMON RAMÓN gAXIOLA-ROBLEs CLAUDIA J.heRNÁNDEZ-CAMACHO TANIA ZENTENO-savÍN Centro de Investigaciones Biológicas del Noroeste La Paz23096México Hospital general de Zona No.1 Instituto Mexicano del Seguro SocialLa Paz23000México Centro Interdisciplinario de Ciencias Marinas Instituto Politécnico NacionalLa Paz23096México

Marine mammals undergo cycles of tissue ischemia and reperfusion during the dive *** injury can result in oxidative tissue damage and the activation of a pro-inflammatory immune *** risk of oxidative damage is reduced by *** hypothesis is that the reported higher antioxidant defenses within marine mammal tissues provide additional protection in situations that produce oxidative stress,like inflammation,in comparison to terrestrial mammal *** were isolated from the whole blood of Pacific bottlenose dolphins(Tursiops truncatus gilli)and humans(Homo sapiens)and were exposed to lipopolysaccharides(LPs,10μg/mL)in vitro to simulate a pro-inflammatory *** stress indicators,including superoxide radical(O_(2)^(•−))production,activities of superoxide dismutase(sOD),catalase(CAT),glutathione peroxidase(gPx),glutathione reductase(gR),and glutathione s-transferase(gsT),as well as oxidative protein damage,were quantified by *** 48 h under experimental conditions,bottlenose dolphin leukocytes produced 1.9 times more O_(2)^(•−)but displayed 2.0 times lower protein carbonyl concentrations compared to human *** 48 h under experimental conditions,bottlenose dolphin leukocytes displayed 7.9,2.0,11.1,and 3.3 times more activities of CAT,gPx,gR,and gsT,respectively,compared to human *** results suggest that,under cell culture conditions,the antioxidant defenses in bottlenose dolphin leukocytes provide additional protection against pro-inflammatory challenges in comparison to human leukocytes,likely as an adaptive advantage.

关键词： Antioxidant defenses Marine mammals Oxidative stress Reactive oxygen species White blood cells

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Differences in arsenic, molybdenum, barium, and other physicochemical relationships in groundwater between sites with and without mining activities

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Natural science 2013年第2期5卷 238-243页

作者： Lia Méndez-Rodríguez Tania Zenteno-savín Baudilio Acosta-Vargas Jobst Wurl Miguel Imaz-Lamadrid Department of Marine geology Autonomous University of Baja California Sur La Paz México Environmental Planning and Conservation Centro de Investigaciones Biológicas del Noroeste S.C. La Paz México

The characteristic relationships of trace metals and other water quality parameters in a specific region can be affected by anthropogenic activeties. since the mid-18th century in the southwestern part of the Baja California Peninsula, intermittent gold mining activities have been developed. We analyzed 36 water quality parameters in accordance with the procedures suggested by international agencies to evaluate the impact of this activity and the time of year on the mobilization of trace element levels and their relationships in groundwater. Quantifiable levels of molybdenum help to establish the area influenced by ore deposits because it is one of the three elements in the paragenesis associated to gold. Arsenic in sites closer to ore burning areas was associated with cobalt, indicating the potential presence of a by-product generated from arsenolite;whereas in the non-mineralized area, it was associated with barium, forming a compound that tends to precipitate, thereby maintaining a natural geochemical control in this region. From the sites sampled, 45% exceeded the limit for arsenic (10 μg/l) established by international agencies. During area monitoring with annual precipitation of 207 mm, only seven of 36 parameters analyzed showed significant differences in relation to time of year.

关键词： Arsenic Barium Molybdenum Water Quality Ore Deposits

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Fungal diversity notes 1611-1716: taxonomic and phylogenetic contributions on fungal genera and species emphasis in south China

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Fungal Diversity 2023年第5期122卷 161-403页

作者： Indunil C.senanayake Walter Rossi Marco Leonardi Alex Weir Mark McHugh Kunhiraman C.Rajeshkumar Rajnish K.Verma samantha C.Karunarathna saowaluck Tibpromma Nikhil Ashtekar sreejith K.Ashtamoorthy sanjay Raveendran gurmeet kour Aishwarya singh saúl De la Peña-Lastra Antonio Mateos Miroslav kolařík Vladimír Antonín HanaŠevčíková Fernando Esteve-Raventós Ellen Larsson Fermín Pancorbo gabriel Moreno Alberto Altés Yolanda Turégano Tian-Ye Du Li Lu Qi-Rui Li Ji-Chuan Kang sugantha gunaseelan Kezhocuyi Kezo Malarvizhi Kaliyaperumal Jizhen Fu Milan C.samarakoon Yusufjon gafforov shakhnoza Teshaboeva Pradeep C.Kunjan Arya Chamaparambath Adam Flakus Javier Etayo Pamela Rodriguez-Flakus Mikhail P.Zhurbenko Nimali Ide silva Danushka s.Tennakoon KPDeepna Latha Patinjareveettil Manimohan KNAnil Raj Mark s.Calabon Abdollah Ahmadpour Zeinab heidarian Zahra Alavi Fatemeh Alavi Youbert ghosta Razmig Azizi Mei Luo Min-Ping Zhao Nuwan D.Kularathnage Li Hua Yun-Hui Yang Chun-Fang Liao Hai-Jun Zhao Anis s.Lestari subashini C.Jayasiri Feng-Ming Yu Lei Lei Jian-Wei Liu Omid Karimi song-Ming Tang Ya-Ru sun Yong Wang Ming Zeng Zin H.Htet Benedetto T.Linaldeddu Artur Alves Alan J.L.Phillips Carlo Bregant Lucio Montecchio AndréDe Kesel Vincent P.Hustad Andrew N.Miller Anna g.Fedosova Viktor Kučera Mubashar Raza Muzammil Hussain Yan-Peng Chen Vinodhini Thiyagaraja Deecksha gomdola Achala R.Rathnayaka Asha J.Dissanayake Nakarin suwannarach sinang Hongsanan sajeewa s.N.Maharachchikumbura Lakmali s.Dissanayake Nalin N.Wijayawardene Rungtiwa Phookamsak saisamorn Lumyong E.B.gareth Jones Neelamanie Yapa Dhanushka N.Wanasinghe Ning Xie Mingkwan Doilom Ishara s.Manawasinghe Jian-Kui(Jack)Liu Qi Zhao Biao Xu Kevin D.Hyde Jiage song Innovative Institute for Plant health/Key Laboratory of green Prevention and Control On Fruits and Vegetables in south China Ministry of Agriculture and Rural AffairsZhongkai University of Agriculture and EngineeringHaizhu DistrictGuangzhou 510225GuangdongChina Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand guangdong Provincial Key Laboratory for Plant Epigenetics College of Life Science and OceanographyShenzhen University3688Nanhai AvenueNanshanShenzhen 518055GuangdongChina sect.Environmental sciences Department MeSVAUniversity of L’AquilaVia Vetoio67100 CoppitoAQItaly Department of Environmental Biology SUNY-ESF241 Illick Hall1 Forestry DriveSyracuseNY 13210USA National Fungal Culture Collection of India MACS Agharkar Research InstitutePuneMaharashtra 411004India Department of Plant Pathology Punjab Agricultural UniversityLudhianaPunjab 141004India Center for Yunnan Plateau Biological Resources Protection and Utilization College of Biological Resource and Food EngineeringQujing Normal UniversityQujing 655011YunnanChina Department of Forest Ecology Kerala Forest Research InstitutePeechiThrissurKerala 680653India Department of Botany The American CollegeTallakkulamMaduraiTamil Nadu 625002India Department of Zoology Savitribai Phule Pune UniversityGaneshkhind RdGaneshkhindPuneMaharashtra 411007India University of santiago de Compostela 15782 Santiago de CompostelaSpain sociedad Micológica Extremeña C/Sagitario 1410001 CáceresSpain Institute of Microbiology of the Czech Academy of sciences Vídeňská108314220 Prague 4Czech Republic Department of Botany Moravian MuseumZelnýtrh 665937 BrnoCzech Republic Department of Life sciences(Botany Unit) University of Alcalá28805 Alcaláde HenaresMadridSpain Biological and Enviromental sciences University of Gothemburgand Gothemburg Global Biodiversity CentreBox 46140530 GöteborgSweden Madrid Mycological society Real Jardín BotánicoC/Claudio Moyano 128014 MadridSpain state Ke

This article is the 15th contribution in the Fungal Diversity Notes series,wherein 115 taxa from three phyla,nine classes,28 orders,48 families,and 64 genera are *** taxa described and illustrated in the present study include a new family,five new genera,61 new species,five new combinations,one synonym,one new variety and 31 records on new hosts or new geographical *** *** introduced and accommodated in *** new genera introduced in this study are Ageratinicola,Kevinia,Pseudomultiseptospora(Parabambusicolaceae),Marasmiellomycena,and Vizzinia(Porotheleaceae).Newly described species are Abrothallus altoandinus,Ageratinicola kunmingensis,Allocryptovalsa aceris,Allophoma yuccae,Apiospora cannae,***,***,Boeremia wisteriae,Calycina papaeana,Clypeo-coccum lichenostigmoides,Coniochaeta riskali-shoyakubovii,Cryphonectria kunmingensis,Diaporthe angustiapiculata,***,***,Diatrypella guangdongense,Dothiorella franceschinii,Endocalyx phoenicis,Epicoc-cum terminosporum,Fulvifomes karaiensis,***,ganoderma ghatensis,Hysterobrevium baoshanense,Inocybe avellaneorosea,***,Jahnula oblonga,Kevinia lignicola,Kirschsteiniothelia guangdongensis,Laboulbenia caprina,***,***,***,***,***,***,***,***,Laccaria rubriporus,Lasiodiplodia morindae,Lyophyllum agnijum,Marasmiellomycena pseudoomphaliiformis,Melomastia beihaiensis,Nemania guangdongensis,Nigrograna thailandica,Nigrospora ficuum,Oxydothis chinensis,***,Petriella thailandica,Phaeoacremonium chinensis,Phialocephala chinensis,Phytophthora debattistii,Polyplosphaeria nigrospora,Pronectria loweniae,seriascoma acutispora,setoseptoria bambusae,stictis anomianthi,Tarzetta tibetensis,Tarzetta urceolata,Tetraploa obpyriformis,Trichoglossum beninense,and Tricoderma *** provide an emendation for Urnula ailaoshanensis Agaricus duplocingulatoides *** i

关键词： Agaricomycetes Ascomycota Basidiomycota Dothideomycetes Eurotiomycetes Fungal diversity geoglossomycetes geographical distribution Host records Incertae sedis Laboulbeniomycetes Lecanoromycetes Leotiomycetes Novel taxa New combination Oomycota Peronosporea Pezizomycetes Phylogeny sordariomycetes south Chinese fungi Taxonomy

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Fission-fusion dynamics of a pelagic delphinid in the arctic:the white-beaked dolphin(Lagenorhynchus albirostris)

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Integrative Zoology 2021年第4期16卷 512-526页

作者： Chiara g.BERTULLI Marianne H.RAsMUssEN Massimiliano ROssO Department of Life and Environmental sciences University of IcelandReykjavikIceland sea Watch Foundation New QuayUK Húsavík Research Centre University of IcelandHúsavíkIceland CIMA Research Foundation SavonaItaly

Delphinids exhibit great variability in their social *** is therefore important to document lesser known species,in extreme changing habitats,to compare and contrast mechanisms driving ***,we describe the first long-term assessment of social structure of white-beaked dolphins(Lagenorhynchus albirostris)using a compiled version of sOCPROg 2.8 and an 11-year photo-identification dataset(2002–2013)collected from whalewatching vessels at 2 sites(Faxaflói and skjálfandi bays)off *** identified a total of 487 dolphins which are suggested to be part of an open population as shown by the discovery curve obtained analyzing photo-id *** social analyses were restricted to 35 adults which were sighted on≥5 different *** mean residency time of white-beaked dolphins in our 2 study areas was of 95 days(sE=35.63;95%CI:23-171),with the“migration-full interchange”model best describing movements of dolphins in and out of our 2 study *** differentiation in this population was high(Likelihood:s=0.87,sE=0.04;r=0.31,sE=0.03)indicating diverse,non-random social *** associations best fit the model of“casual acquaintances”against the standardized lagged association rates with the majority of white-beaked dolphin associations being short-term,but with a few long-term across *** for preferred companionship,long-term associations are favored over *** this study,associations among white-beaked dolphins are short-term but with desirably long-term associations fitting into a society with fission-fusion *** information expands the latitudinal range for which social structure has been described for oceanic dolphin species.

关键词： association patterns Iceland Lagenorhynchus albirostris social structure white-beaked dolphin

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Issues Relating to standards for Determination of Design Patent Infringement——Comments on and analysis of patent infringement case (spanish) Fabricacion Asientos Vehiculos Industriales *** United Automobile Industry (suzhou) Co.,Ltd.

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中国专利与商标 2008年第4期 40-44页

作者： Zhou Xiaobing Judge of the Intellectual Property Tribunal of the Beijing No.2 Intermediate People’s Court

The case in brief The plaintiff,the spanish Fabricacion Asientos Vehicu- los Industriales sA(FAINsA),was granted,by the state In- tellectual Property Office(sIPO),the patent right for the de- sign of the'seats for vehicles'in March *** 2007,the FAINsA found out that the seats inside the Haige-brand coaches made and marketed by the Kinglong United Auto- mobile Industry(suzhou)Co.,Ltd.(Kinglong for short)and marketed by the Beijing Jintongbaolong Automobile sale

关键词： Issues Relating to standards for Determination of Design Patent Infringement Ltd spanish suzhou Fabricacion Asientos Vehiculos Industriales sav.Kinglong United Automobile Industry Design case

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WATERsheD AND EsTUARINE LANDsCAPE CHARACTERIsTICs AND TheIR EFFECTs ON ECOLOgICAL REsPONsEs OF ChesAPEAKE BAY sUBEsTUARIEs

WATERSHED AND ESTUARINE LANDSCAPE CHARACTERISTICS AND THEIR ...

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第三世界生态高峰会

作者： King Ryan s. Weller Donald E. Hines Anson H. smithsonian Enviromnental Researck Center.Edgewater MD 21037 UsA Department of Biology Baylor University Waco TX 76798.UsA smithsonian Enviromnental Researck Center.Edgewater MD 21037 UsA smithsonian Enviromnental Researck Center.Edgewater MD 21037 UsA

The distribution of biota in estuaries is influenced by watershed and estuary *** used landscape analysis to identify the linkages of several estuarine responses with watershed and estuarine characteristics for sballow subestuaries of Chesapeake Bay,UsA and their local watersheds We analyzed the effects of watershed use and subestuary characteristics on submerged aquatic vegetation(sav) abundance in 101 subestuaries during 1984-2003.A classification regression tree(CART)model indicated that 60% of the variance in sav abundance could be explained by subestuary fractal dimension, mean tidal range,local watershed land use,watershed to subestuary area ratio,and mean wave ***-measures ANOVA showed that sav abundance declined with the dominant watershed land use in the order:forested,mixed-undisturbed,mixed-developed＞ mixed-agricultural＞agricultural＞*** point analyses indicated strong threshold responses of sav abundance to the proportion of developed land,point source nutrient inputs and the density of septic systems in the *** sampled 19 subestuaries to test hypothesized relationships of blue crabs(Callinectes sapidus) and bivalves(Macoma balthica and ***)with landscape *** measured water quality,sediment class,physical habitat characteristics,and adjacent land use at each of six stations within each *** nets were employed to estimate blue crab abundance and size-structure,while cores were used to estimate bivalve densities and *** analysis indicated that 51% of the variance in blue crab abundance could be explained by salinity, watershed land use and shoreline marsh *** related subestuary average measurements of total Polychlorinated biphenyl(PCB) concentrations in white perch(Morone americana)to the amount and spatial arrangement of developed land in watersheds that discharge into 14 *** considered the intensity of development in watersheds using four develo

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福建省1株salivirus病毒全基因组特征分析

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中国公共卫生 2021年第10期37卷 1535-1539页

作者：何文祥吴冰珊林琦陈炜翁育伟福建省疾病预防控制中心福建省人兽共患病研究重点实验室福州350001

目的了解福建地区salivirus病毒的全基因组特征并对其遗传背景进行分析。方法对2018年福建省342份腹泻儿童粪便标本中检测到的1份salivirus病毒阳性标本进行高通量测序,获得病毒全基因组序列FUAN-01。对FUAN-01全基因组核苷酸序列和VP1... 详细信息

目的了解福建地区salivirus病毒的全基因组特征并对其遗传背景进行分析。方法对2018年福建省342份腹泻儿童粪便标本中检测到的1份salivirus病毒阳性标本进行高通量测序,获得病毒全基因组序列FUAN-01。对FUAN-01全基因组核苷酸序列和VP1区的核苷酸及氨基酸进行遗传进化分析。结果 FUAN-01全长7907 bp。全基因组与参考株的核苷酸同源性为81.86%~98.21%,与KT182636株的同源性最高,二者ORF区(开放阅读框)编码蛋白存在14个氨基酸位点的差异。VP1区与参考株的核苷酸酸同源性为76.83%~97.81%,与KT182636株的同源性最高;氨基酸同源性为85.27%~99.22%,与KT182636株同源性最高。FUAN-01属于salivirus A1(sav-A1)基因型。结论首次在福建地区腹泻儿童粪便中发现salivirus。国内sav-A1株或由同一来源传入,与欧洲来源的病毒可能存在流行病学关联。需建立可靠的筛查办法,进一步对福建地区salivirus的病原学及流行病学情况展开研究。

关键词： salivirus 全基因组遗传进化分析

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基于R-ET融合的黄河流域水资源管理和调控案例研究

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水利水电技术 2014年第2期45卷 1-4,9页

作者：冯峰何宏谋陈召军张文鸽黄河水利职业技术学院河南开封475004 清华大学水利系北京100084 黄河水利科学研究院河南郑州450003 开封黄河河务局河南开封475002

针对目前黄河流域存在的实际引黄耗水总量超过年度分水指标、地下水超采等问题,分析其原因在于水资源管理主要侧重于供水管理,调控的是取水量,忽视了水资源在使用过程中的循环转化与消耗,因此提出基于R-ET(Runoff-Evapotranspiration/... 详细信息

针对目前黄河流域存在的实际引黄耗水总量超过年度分水指标、地下水超采等问题,分析其原因在于水资源管理主要侧重于供水管理,调控的是取水量,忽视了水资源在使用过程中的循环转化与消耗,因此提出基于R-ET(Runoff-Evapotranspiration/径流—土壤水分蒸发蒸腾损失总量)融合的新型水资源管理模式,以"耗水"管理替代"取水"管理,控制区域ET从而实现真实节水。以黄河流域兰州至头道拐区间为研究实例,通过分析各分项ET(Evapotranspiration/土壤水分蒸发蒸腾损失总量)调控的可行性,提出了4种节水方案,可使引黄水量从无节水的98.6亿m3减少至42.96亿m3,从而达到了降低该区域实际耗水量的目的。该管理模式有利于完善黄河流域的水资源管理体系,为黄河的健康可持续发展奠定基础。

关键词：黄河流域水资源管理耗水量案例研究 R-ET(Runoff-Evapotranspiration)

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