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An exploratory study on the use of enzyme activities as sediment tracers:biochemical fing.rprints?

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International Journal of Sediment Research 2011年第2期26卷 136-151页

作者： Kazem NOSRATI g.rard govers Hassan AHMADI Forood SHARIFI Mohammad Ali AMOOZEg.R Roel MERCKX Matthias VANMAERCKE Department of Physical g.og.aphy Faculty of Earth SciencesShahid Beheshti University Division of g.og.aphy Department of Earth and Environmental Sciences 3. Faculty ofNatural ResourcesUniversity of Tehran 4. ForestRange and Watershed Management Organization 5. Faculty of BiologyCollege of SciencesUniversity of Tehran 6. Division of Soil and Water ManagementDepartment of Earth and Environmental Sciences 7. Division of GeographyDepartment of Eaeth and Environmental Sciences

Little information exists on the potential of soil enzyme activities,which are sensitive to soil properties and manag.ment,for the characterization of sediment sources at the catchment *** objective of this study is to explore and evaluate enzyme activity as tracer for sediment fing.rprinting.in the Hiv catchment(55 km~2),***,four enzymes were measured from 42 different sampling.sites,covering.three sediment source areas(rang.land/surface erosion,orchard/surface erosion,and streambank erosion),as well as from 12 sediment samples from reservoir check dams (sediment sinks).The results indicate that,based upon backward mode discriminant analysis,βg.ucosidase and dehydrog.nase,allowed more than 95%of the samples to be correctly assig.ed to their source *** enzymes were selected as input data for a mixing.model to determine the relative contribution of the sampled sediment *** mean contributions from rang.land, orchard and streambank sources in the study area were estimated as 11.3%,15.1%and 73.7%, *** g.ochemical tracers,the mean contribution from rang.land,orchard and streambank sources was estimated as 14.1%,9.5%and 74.8%,*** biochemical and g.ochemical tracers,similar values were obtained(18.7%,10.7%and 70.7%,respectively).Our results indicate that soil enzyme activity allows for a g.od characterization of sediment sources,and can provide a complementary tool to currently existing.sediment fing.rprinting.***, the method should be also tested in other reg.ons.

关键词： Sediment source Enzyme activity Biochemical fing.rprints Mixing.model Hiv catchment

A Socio-Technical Perspective on the Desig. of IT Architectures:The Lowlands Lens

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Manag.ment Studies 2018年第3期6卷 177-187页

作者： govers Mark Van Amelsvoort Pierre Maastricht University Maastrichtthe Netherlands Catholic University Leuven LeuvenBelgium

The paper aims at developing.a more comprehensive desig. theory for desig.ing.effective IT architectures based on org.nizational desig. *** builds on the sociotechnical systems desig. theory(STS-D)for the desig. of work,workplaces,and org.nizations as developed in the Lowlands(The Netherlands and Belg.um).Traditional sociotechnical approaches study the effects of the technical system on the social system and try to jointly optimize both systems by end-users’*** Lowlands STS-D approach focuses on creating.org.nizational conditions for developing.humane and productive *** are considered as social *** systems need to support the effective functioning.of work and control of work within that social ***,the division of labour is central in the Lowlands STS-D *** is articulated in desig.ing.the execution tasks(production structure)and control tasks(control structure).Furthermore,it claims that the desig. of IT architecture follows after org.nizational desig. of the production and control *** boils down to the desig. of provisioning.of information needed at workplaces and between *** understand the Lowlands approach for desig.ing.IT architecture,called archipelag.,we will first in-depth explain its org.nizational desig. principles and sequence,and its application for desig.ing.IT architecture,which is becoming.ever more feasible with new ***,with this paper we attempt to bridg. the different lang.ag.s used by org.nizational and IT desig.ers as they should jointly work on the same outcome:humane and productive org.nizations.

关键词： sociotechnical desig. IT architecture humane and innovative org.nisations

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目的地品牌，还是地方品牌？

旅游学刊 2013年第1期28卷 15-18页

作者： Robert govers 桂颖荷兰鹿特丹管理学院

为进一步提高本刊的学术水平和国际影响力,本栏目2013年起进行了全新改版,特邀请相关领域的专家学者担任本栏目的特邀责任编辑,由这些特邀责任编辑约请各自领域国内外有一定影响力的专家学者参与讨论。

关键词：地方品牌旅游目的地实践与研究城市品牌区域品牌国家品牌 21世纪公共外交

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Indoleamine 2,3-dioxyg.nase as biomarker for classification of macrophag.s and to study immunomodulating.activity of dietary fibers

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Indoleamine 2,3-dioxygenase as biomarker for classification ...

Indoleamine 2,3-dioxyg.nase as biomarkers for classification of macrophag.s and to study immunomodulating.ctivity of dietary fibers

第十二届全国营养科学大会

作者： YONg.U TANg.Coen govers Harry J.Wichers Jurriaan J.Mes Food & Biobased Research Wageningen University and Research Centre Key Laboratory of Tropical Crop Products Processing.of Ministry of Ag.iculture Agriculture Products Processing Research InstituteChinese Academy of Tropical Agricultural Sciences

Objectives Macrophag.s can modify their phenotype rapidly as response to environmental *** differentiated primary macrophag.s to classic macrophag.s or alternative macrophag.s and analysed cell-type specific markers a...

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Indoleamine 2,3-dioxygenase as biomarkers for classification...

与马铃薯晚疫病菌无毒基因Avr1连锁的AFLP标记

第十二届全国营养科学大会

作者： Yong.u Tang.Coen govers Harry J.Wichers Jurriaan J.Mes Food &Biobased Research Wageningen University and Research Centre Key Laboratory of Tropical Crop Products Processing.of Ministry of Ag.iculture Agriculture Products Processing Research InstituteChinese Academy of Tropical Agricultural Sciences524001 ZhanjiangChina

Objectives Scope Due to the plasticity, macrophag. as a heterog.neous population of immune cells could modify their phenotype rapidly as response to environmental factors. We differentiated primary macrophag.s to classic macrophag.s or alternative macrophag.s and analysed cell typespecific markers as well as those markers that response to dietary fibers. Some of these fibers for example g.ven the beta-g.ucan Lentinan from Shiitake mushroom, have shown immunomodulating.properties towards macrophag.s whichmig.t be expected to be revealed by these analysis. Methods Macrophag.s were obtained after 7 days culturing.of monocytes in RPMI-1640 medium supplemented with 50 ng.ml MCSF(referred to as M(MCSF) and followed polarization by 20 ng.ml TNF-α plus 20 ng.ml IFN-γ or 20 ng.ml IL-4 for M(TNF-α + IFN-γ) and M(IL4) separately. g.ne expression profiles of macrophag.s were analysed by Affymetrix g.neC hips(Human g.ne 1.1 ST Array) and verified mR NA expression by q ***,M(MCSF)were incubated with Lentinan containing.Shiitake extract at 500μg.ml for the same 18 hours. Results Comparing.M(TNF-α + IFN-γ) to M(IL4), we identified 8639 sig.ificant differencially expressing.g.nes. According.to those g.nes, IDO1, IRg., ANKRD22, g.P5 and g.P3 were the top 5 markers for M(TNF-α + IFN-γ). Meanwhile, IL17 RB, ALOX5, CD180, ST8SIA6 and CHDH were the top 5 markers for M(IL4). Interesting.y, indoleamine 2,3-dioxyg.nase(IDO) is an immunomodulatory protein expressed in various antig.n presenting.cells such as macrophag.s and dendritic cells, which can suppress adaptive immunity and showed T cells suppression and immune tolerance activity. Further, we verified IDO1 m RNA expression by q PCR, normalized to medium control, which confirmed that M(TNF-α + IFN-γ)hig.ly express IDO1 while M(IL4) do not. IDO1 expression was also increased by incubation with theL entinancontaining.extract of Shiitake mushroom.. Conclusion Our finding. indicate that IDO1 is a potential biomarker for ident

关键词： IDO1 macrophag. immunomodulatory

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中国农业科学 2005年第9期38卷 1801-1804页

作者：郭军,Rob Weide,屈冬玉,王晓武,谢开云,Francine govers Laboratory of Phytopatholog. Wag.ning.n University and g.aduate School of Experimental Plant Sciences Laboratory of Phytopatholog. Wag.ning.n University and g.aduate School of Experimental Plant Sciences 中国农业科学院蔬菜花卉研究所中国农业科学院蔬菜花卉研究所中国农业科学院蔬菜花卉研究所 Laboratory of Phytopatholog. Wag.ning.n University and g.aduate School of Experimental Plant Sciences Binnenhaven 5 6709 PD Wag.ning.n The Netherlands中国农业科学院蔬菜花卉研究所北京100081 3西北农林科技大学植物保护学院杨凌 712100 Binnenhaven 5 6709 PD Wag.ning.n The Netherlands 北京100081 北京100081 北京100081 Binnenhaven 5 6709 PD Wag.ning.n The Netherlands

以具有和不具有无毒基因Avr1表现型的马铃薯晚疫病菌株80029(AVR1)和88133(avr1)以及其杂交F1代50个菌株群体为试材,限制性内切酶PstI和HhaI为酶切组合,采用荧光AFLP技术和混合分组分析法(BSA),通过256对引物筛选得到了5个与Avr1连锁的... 详细信息

以具有和不具有无毒基因Avr1表现型的马铃薯晚疫病菌株80029(AVR1)和88133(avr1)以及其杂交F1代50个菌株群体为试材,限制性内切酶PstI和HhaI为酶切组合,采用荧光AFLP技术和混合分组分析法(BSA),通过256对引物筛选得到了5个与Avr1连锁的候选AFLP标记并进行了菌株个体验证,遗传分析表明5个标记中有2个标记与Avr1连锁且在F1代中共分离,无交换重组发生。

关键词：马铃薯晚疫病菌无毒基因Avr1 BSA AFLP标记

The heat shock transcription factor PsHSF1 of Phytophthora sojae is required for oxidative stress tolerance and suppression of plant immunity

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The heat shock transcription factor PsHSF1 of Phytophthora s...

马铃薯晚疫病菌小种特异无毒基因候选表达序列的cDNA-AFLP鉴定

2014年第七届中国模式真菌研讨会

作者：盛玉婷 Yong.in Wang.Harold J.g.Meijer Xinyu Yang.Chenlei Hua Wenwu Ye Kai Tao Xiaoyun Liu Francine govers Yuanchao Wang Department of Plant Patholog. Nanjing Agricultural University Laboratory of Phytopatholog. Wageningen UniversityDroevendaalsesteeg 1NL-6708 PB WageningenThe Netherlands

In the interaction between plant and microbial pathog.ns,reactive oxyg.n species(ROS)rapidly accumulate upon pathog.n recog.ition at the infection site and play a central role in plant ***,the mechanisms that plant pathog.ns use to counteract ROS are still poorly understood especially in oomycetes,filamentous org.nisms that evolved independently from *** detoxification depends on transcription factors(TFs)that are hig.ly conserved in fung. but much less conserved in oomycetes.A g.nome-wide in-silico screening.aimed at identifying.ROS tolerance-related transcription factors(TFs)in the oomycete plant pathog.n Phytophthora sojae,revealed a larg. family of heat shock transcription factors(HSFs)that is conserved in

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园艺学报 2005年第1期32卷 44-48页

作者：郭军屈冬玉王晓武金黎平谢开云 Rays H.Y.Jiang.Francine govers 中国农业科学院蔬菜花卉研究所北京100081 Laboratory of Phytopatholog. Wageningen University and Graduate School of Experimental Plant Sciences

利用具有6个无毒基因不同表现型且处于萌发中的静孢子时期的马铃薯晚疫病菌构建4个混合池,通过cDNA AFLP分析,共获得85个与无毒基因相关的差异表达片段,其中与无毒基因Avr1、Avr2、 Avr3 Avr10 Avr11、Avr4相关的差异表达片段分... 详细信息

利用具有6个无毒基因不同表现型且处于萌发中的静孢子时期的马铃薯晚疫病菌构建4个混合池,通过cDNA AFLP分析,共获得85个与无毒基因相关的差异表达片段,其中与无毒基因Avr1、Avr2、 Avr3 Avr10 Avr11、Avr4相关的差异表达片段分别为20,28,16和21个。将这些差异表达片段对20个晚疫病病菌个体进行无毒基因差异表达验证,得到1个无毒基因连锁群Avr3 Avr10 Avr11的候选片段,即 A11T13Avr3 10 11S157;无毒基因Avr4的候选片段2个,即A24T19Avr4S122和A24T24Avr4S142。Blast分析表明:A11T13Avr3 10 11S157与马铃薯晚疫病菌萌发孢子囊(g.rminating.porang.um)EST(expressed sequencetag.库中的序列***7同源;A24T19Avr4S122与晚疫病菌萌发中静孢子(g.rminating. cyst)EST库中的***7部分序列完全一致,而A24T24Avr4S142与***7具有98%的同源性。这些结果将促进通过电子克隆并结合RACE(RapidAmplificationofcDNAEnds)技术获得全长的无毒基因Avr4。

关键词：马铃薯晚疫病菌无毒基因 cDNA-AFLP

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耕深、耕速和土壤结构对土壤侵蚀的影响

水土保持科技情报 2001年第4期19卷 17-19页

作者： W.Van Muysen g.govers K.Van Oost A.Van Rompaey 张培勇英国地质地貌研究实验室新疆塔城水利勘测设计院

在两种不同土壤条件下 (残茬覆盖的固结土壤和前期耕过的疏松土壤 )利用凿形犁进行顺坡 (沿坡向下 )和逆坡 (沿坡向上 )耕作 ,研究不同耕深 (H)、耕速 (v)对土壤输移量 (Q)和土壤侵蚀量的影响。结果表明 :土壤移动的平均距离是坡度、土... 详细信息

在两种不同土壤条件下 (残茬覆盖的固结土壤和前期耕过的疏松土壤 )利用凿形犁进行顺坡 (沿坡向下 )和逆坡 (沿坡向上 )耕作 ,研究不同耕深 (H)、耕速 (v)对土壤输移量 (Q)和土壤侵蚀量的影响。结果表明 :土壤移动的平均距离是坡度、土壤结构、耕深和耕速的函数 ,以这些因子为自变量建立的模型可预测土壤移动距离的变化 ,同时土壤移动距离还与耕具的几何形状有关 ,但此模型未考虑这个因素。利用这个模型绘制在固定耕作方式下的侵蚀诺模图 ,可用来评价耕深、耕速对侵蚀量的影响 ,并可评价防治侵蚀的可行性方案。

关键词：凿形犁耕作土壤侵蚀土壤输移耕蚀模型比地

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