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Lineage relationship of CD8^＋ T cell subsets is revealed by progressive changes in the epigenetic landscape

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Cellular & Molecular Immunology 2016年第4期13卷 502-513页

作者： Joseph g. Crompton Manikandan Narayanan Suresh Cuddapah Rahul Roychoudhuri Yun Ji Wenjing Yang Shashank J. Patel Madhusudhanan Sukumar Douglas C. Palmer Weiqun Peng Ena Wang Francesco M. Marincola Christopher A. Klebanoff Keji Zhao John S. Tsang Luca gatt-inoni Nicholas P. Restifo Surgery Branch National Cancer Institute National Institutes of Health Bethesda MD 20892 USA cambridge Institute for Medical Research University of Cam bridge Cambridge CB20XY UK Systems genomics and Bioinformatics Unit Laboratory of Systems Biology National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda MD 20892 USA Department of Environmental Medicine New York University School of Medicine Tuxedo NY 10987 USA Experimental Transplantation and Immunology Branch National Cancer Institute National Institutes of Health Bethesda MD 20892 USA Department of Physics The George Washington University Washington DC 20052 USA Research Branch Sidra Medical and Research Centre Doha Qatar Infectious Disease and Immunogenetics Section Department of Transfusion Medicine Clinical Center National Institutes of Health Bethesda MD 20892 USA Clinical Investigator Development Program NCI/NIH Bethesda MD 20892 USA Systems Biology Center National Heart Lung and Blood Institute National Institutes of Health Bethesda MD 20892 USA. Lombardi Comprehensive Cancer Center Georgetown University Washington. DC 20007. United States. Bethesda. MD 20892. USA.

To better elucidate epigenetic mechanisms that correlate with the dynamic gene expression program observed upon T-cell differentiation, we investigated the genomic landscape of histone modifications in naive and memory CD8＋ T cells. Using a ChlP-Seq approach coupled with global gene expression profiling, we generated genome-wide histone H3 lysine 4 （H3K4me3） and H3 lysine 27 （H3K27me3） trimethylation maps in naive, T memory stem cells, central memory cells, and effector memory cells in order to gain insight into how histone architecture is remodeled during T cell differentiation. We show that H3K4me3 histone modifications are associated with activation of genes, while H3K27me3 is negatively correlated with gene expression at canonical loci and enhancers associated with T-cell metabolism, effector function, and memory. Our results also reveal histone modifications and gene expression signatures that distinguish the recently identified T memory stem cells from other CD8＋ T-cell subsets. Taken together, our results suggest that CD8＋ lymphocytes undergo chromatin remodeling in a progressive fashion. These findings have major implications for our understanding of peripheral T-cell ontogeny and the formation of immunological memory.

关键词： TRANSCRIPTION FACTOR STEM-CELLS MEMORY EFFECTOR DIFFERENTIATION BET FATE LYMPHOCYTES MAINTENANCE gENERATION

Early Extubation in Congenital Heart Surgery

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Early Extubation in Congenital Heart Surgery

The Fifth China International Congress on Thoracic and Cardiovasculal Surgery

作者： R.Neirotti D.Jones R.Hackbarth g.Fosse T.Steffens M.Oosterheert DeVos Children’s Hospital 100 Michigan N.E.MC-117Grand RapidsMI 49503USA DeVos Children’s Hospital 100 Michigan N.E.MC-117Grand RapidsMI 49503USA DeVos Children’s Hospital 100 Michigan N.E.MC-117Grand RapidsMI 49503USA DeVos Children’s Hospital 100 Michigan N.E.MC-117Grand RapidsMI 49503USA DeVos Children’s Hospital 100 Michigan N.E.MC-117Grand RapidsMI 49503USA DeVos Children’s Hospital 100 Michigan N.E.MC-117Grand RapidsMI 49503USA

＜正＞ Objective:To evaluate the impact of early extubation（within six hours post-operatively）onchildren undergoing congenital heart *** of the problems following surgery are related tothe endotracheal tube,mechanical ventilation,and interventions necessary to maintain ***-ods:717 consecutive patients from 4/93 t0 1/98 were reviewed.66 premature infants with isolatedductus ligation and five patients with preexisting respiratory conditions precluding extubation were *** records were *** data on 638 patients was evaluated using ***:Extubation occurred within six hours in 81.5%（76.2% in the operating room）.Median agewas 22 *** weight was 11kg.

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一种高速铣削刀轨优化算法的研究与实现

淮阴工学院学报 2003年第1期12卷 17-19页

作者：孙全平廖文和南京航空航天大学CADcam工程研究中心

针对高速加工的特点以及现实要求 ,提出了适合高速铣削的一种刀轨优化算法。经优化的刀轨不仅能适应高速 ,而且能保持机床运转平稳 ,延长刀具寿命。该算法应用于Superman 2 0 0 0CAD cam高速铣加工模块 ,测试表明 ,计算速度快。

关键词：高速切削刀轨优化算法

Late Protective Effects of the Anticalmodulin Drug Fluphenazine on Carbon Tetrachloride-induced Liver Necrosis

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Biomedical and Environmental Sciences 1995年第3期8卷 218-225页

作者： E. C. DE FERREYRA A. S. BERNACCHI M. F. SAN MARTIN g. D. CASTRO AND J. A. CASTRO (Centro de Investigaciones Toxicologicas (CEITOX)-CITEFA/CONICET,Zufriategui 4380, (1603) Villa Martelli, Buenos Aires, Angentina) Centro de Investigaciones Toxicológicas (CEITOX)-CITEFA/CONICET Zufriategui Buenos Aires Argentina

Fluphenazine (FP) treatment (50mg/kg bw, ip in saline) 30 min before or 6 or 10 h after CCl4 administration (1 ml/kg ip in olive oil) significantly prevented the liver necrosis produced by the hepatotoxin at 24 h. FP had enhancing effects on the covalent binding of CCl4 reactive metabolites to cellular constituents and on CCl4 induced lipid *** lowered bOdy temperature of the CCl4-poisoned animals during the 24 h observation period. The obtained results are compatible but do not prove the hypothesis that calmodulin (cam) had participation in late occurring events preceding necrosis. FP lowering action on body temperature, however, might also play a role in the effects of this drug on the onset of CCl4 induced liver necrosis. FP levels in liver tissue as determined by gas chromatography-mass spectrometry evidenced the presence of the drug in amounts suffi cient to inhibit cam and that suggests that not all preventive effects of FP are due to its indirect actions on the central nervous system via decreased body temperature

关键词： ab Late Protective Effects of the Anticalmodulin Drug Fluphenazine on Carbon Tetrachloride-induced Liver Necrosis

The role of shaded cocoa plantations in the maintenance of epiphytic orchids and their interactions with phorophytes

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Journal of Plant Ecology 2020年第1期13卷 27-35页

作者： Jonas Morales-Linares Joséggarcía-Franco Alejandro Flores-Palacios Thorsten Krömer Tarin Toledo-Aceves Red de Ecología Funcional Instituto de EcologíaA.C.Carretera Antigua a Coatepec 351El HayaXalapa 91070VeracruzMexico Centro de Investigación en Biodiversidad y Conservación Universidad Autónoma del Estado de MorelosAv.Universidad 1001Col.ChamilpaCuernavaca 62209MorelosMexico Present address:Centro de Investigación en Biodiversidad y Conservación Universidad Autónoma del Estado de MorelosAv.Universidad 1001Col.ChamilpaCuernavaca 62209MorelosMéxico Centro de Investigaciones Tropicales Universidad VeracruzanaJoséMaría Morelos 44-46Zona CentroXalapa 91000VeracruzMexico

Aims Habitat loss and fragmentation are the main threats to biodiversity in tropical *** such as shaded cocoa plantations(SCP)provide refuge for tropical forest ***,it is poorly known whether the interspecific ecological interactions are also maintained in these transformed *** evaluated the diversity,reproductive status and photosynthetic metabolism(cam or C3)of the epiphytic orchid community,and their interactions with host trees(phorophytes)in SCP compared to tropical rainforest(TRF).Methods In southeastern Mexico,three sites each in TRF and SCP were studied,with four 400 m2 plots established at each site to record all orchids and their *** determined the reproductive(adult)or non-reproductive(juvenile)status of each orchid individual in relation to the presence or absence,respectively,of flowers/fruits(or remnants),and assigned the photosynthetic pathway of each orchid species based in *** used true diversity and ecological networks approaches to analyze orchid diversity and orchid–phorophyte interactions,*** Findings In total,607 individuals belonging to 47 orchid species were *** diversity was higher in TRF(19 effective species)than in SCP(11 effective species)and only seven species were shared between the two *** orchid species were more frequent in SCP(53%)than in TRF(14%).At the community level the proportion of non-reproductive and reproductive orchid species and the nested structure and specialization level of the TRF orchid–phorophyte network were maintained in ***,only a subset of TRF epiphytic orchids remains in SCP,highlighting the importance of protecting *** this difference,shaded agroecosystems such as SCP can maintain some of the diversity and functions of natural forests,since the SCP epiphytic orchid community,mainly composed of cam species,and its phorophytes constitute a nested interaction network,which would confer robustness to d

关键词： agroecosystem ecological networks Mexico photosynthetic pathway tropical rainforest

CAD／cam制作的整体硅酸锂全瓷冠与饰面瓷一氧化锆全瓷冠的比较研究：模拟疲劳试验下的破坏模式与可靠性

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中国口腔医学继续教育杂志 2012年第6期15卷 343-350页

作者： Petra C. guess Ricardo A. Zavanelli Nelson R.F.A. Silva Estevam A. Bonfante Paulo g. Coelho Van P. Thompson 何帅不详南方医科大学口腔医学院广州市510515

目的：本研究的目的在于评估CAD／cam制作的整体硅酸锂全瓷冠与手工饰瓷的氧化锆全瓷冠的体外疲劳表现和可靠性。材料和方法：利用CAD设计并采用快速成型技术制作下颌磨牙进行全冠预备后的基牙模型，通过CAD／cam系统分别完成全解剖式... 详细信息

目的：本研究的目的在于评估CAD／cam制作的整体硅酸锂全瓷冠与手工饰瓷的氧化锆全瓷冠的体外疲劳表现和可靠性。材料和方法：利用CAD设计并采用快速成型技术制作下颌磨牙进行全冠预备后的基牙模型，通过CAD／cam系统分别完成全解剖式的19颗硅酸锂全瓷冠（IPS ***）和21颗手工饰瓷的氧化锆全瓷冠（IPS *** ZirCAD／Ceram）。用树脂粘结材料将上述全瓷冠粘结于前期储存在水中的拟牙本质复合树脂模型上。在模拟口腔运动的疲劳试验中，用半径318mm的碳钨压头从全瓷冠的远颊尖加力并缓慢向舌侧滑动07mm．用三种不同的步进应力方法测试，直到试件发生破坏。当试件出现大块崩瓷或冠折裂时视为失败。如果承受高负载（〉900N）时试件仍未出现失败．则测试方法改变为应力比r呈阶梯式变化的疲劳试验。计算应力水平概率曲线及可靠性。结果：手工饰瓷的氧化锆全冠加载200N负荷完成10万次循环时出现崩瓷，其可靠性〈0．01（0．03～000，双侧90％的可信区间）。在整个步进应力（18万循环，900N）模拟1：3腔运动疲劳试验中未发生CAD／cam制作的整体硅酸锂全瓷冠的失败，在应力比r的疲劳试验（100万循环．100～1000N）中整体硅酸锂全瓷冠也未见失败。1100～1200N负载可能是阈值范围．硅酸锂全瓷冠出现破坏或折裂。结论：基于目前的疲劳试验结果．CAD／cam制作的全解剖式硅酸锂全瓷冠具有较高的抗疲劳特性．而手工饰瓷的氧化锆全瓷冠在较低负载的循环试验过程中易出现崩瓷。

关键词： CAD cam系统氧化锆全瓷冠疲劳试验可靠性硅酸制作模拟饰面瓷

Maternal undernutrition and offspring sex determine birth-weight, postnatal development and meat characteristics in traditional swine breeds

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Journal of Animal Science and Biotechnology 2018年第3期9卷 674-688页

作者： M.Vázquez-gómez C.garcía-Contreras L.Torres-Rovira S.Astiz C.óvilo A.gonzález-Bulnes B.Isabel Faculty of Veterinary Medicine UCMMadrid Spain INIA Madrid Spain

Background: The aim of this study was to determine how maternal undernutrition during pregnancy and offspring birth-weight can affect the postnatal development of offspring under farm conditions, which may lead to consequences in its meat and carcass quality. The current study involved a total of 80 litters from Iberian sows fed a diet fulfilling daily requirements(n = 47; control) or providing 70% daily requirements(n = 33; underfed) from d 38 to d 90 of gestation when fetal tissue development begins. After birth, piglets born live were classified as low birthweight(LBW; g) and normal birth-weight(NBW; ≥1 kg). During the growing phase, 240 control and 230 underfed pigs(50% males and females) distributed by BW category and sex were studied until the ***: At birth and weaning, there were significant differences in all morphological measures and weight between NBW and LBW piglets as expected(P gestational feed restriction. During the growing phase, NBW pigs continued with higher weight than LBW pigs on all the days of evaluation(P gh control-LBW-females and LBW-males showed a catch-up growth. However, underfed pigs showed slower growth and higher feed conversion ratio than control pigs(P ge daily weight gain(ADWg) for the overall period was greater for NBW, male and control pigs than for their LBW, female and underfed pigs(P gs were slaughtered at a younger age than LBW pigs(P ghtering, control pigs also had higher carcass yield and backfat depth than underfed pigs(P ges in the polar lipid fraction of liver and loin. The fatty acid composition of loin in control pigs had higher C18:1 n-9 and n-3 FA concentrations, as well as lower ∑n-6/∑n-3 ratio, than in underfed pigs(P < 0.005).Conclusions: In brief, results showed

关键词： Carcass quality Fatty acids Feed restriction growth Iberian pigs Low birth-weight Malnutrition

循环加载对全解剖式二氧化锆冠抗压强度的影响

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河北医科大学学报 2019年第1期40卷 70-73页

作者：吴硕廉云敏g 高岚王璞河北医科大学第二医院口腔修复科河北石家庄050000

目的观察循环加载对全解剖式二氧化锆冠抗压强度的影响。方法制作18个标准左侧下颌第一磨牙的金属代型,利用CAD/cam技术分别制作出对应的18个全锆冠。用3M玻璃离子将制作完成的18个全锆冠分别黏固于相应金属代型上。随机分成3组,每组6... 详细信息

目的观察循环加载对全解剖式二氧化锆冠抗压强度的影响。方法制作18个标准左侧下颌第一磨牙的金属代型,利用CAD/cam技术分别制作出对应的18个全锆冠。用3M玻璃离子将制作完成的18个全锆冠分别黏固于相应金属代型上。随机分成3组,每组6个。将A组和B组全锆冠分别固定在生物力学试验机上进行循环加载,加载频率为8Hz,循环次数为300 000次,其中A组加载力为300N,B组加载力为500N。随后将18个试件按顺序依次置于万能试验机上测试其抗压强度,记录数值。应用SPSS 21.0软件对实验所得的数据进行统计学分析。结果各组全冠的抗压强度比较差异无统计学意义(P>0.05)。结论使用不同力值对全锆冠进行300 000次循环加载对其抗压强度无明显影响。

关键词：牙冠二氧化锆抗压强度循环加载

意大利头痛诊所收治的进行补充和替代治疗(cam)偏头痛患者的患病率、模式和预测因素

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世界核心医学期刊文摘（神经病学分册） 2005年第10期1卷 10-10页

作者： Rossi P. Di Lorenzo g. Malpezzi M.g. 夏峰 Via Suvereto 250 00139 Rome Italy Dr.

The use of complementary and alternative medicine (cam)- in migraine is a gr owing phenomenon about which little is known. This study was undertaken to evalu ate the rates, pattern and presence of predictors of cam use in a clinical popul ation of patients with different migraine subtypes. Four hundred and eighty- on e migraineurs attending a headache clinic were asked to undergo a physician- ad ministered structured interview designed to gather information on cam use. Past use of cam therapies was reported by 31.4% of the patients surveyed, with 17.1 % having used cam in the previous year. cam therapies were perceived as benefi cial by 39.5% of the patients who had used them. A significantly higher propor tion of transformed migraine patients reported cam treatments as ineffective com pared with patients suffering from episodic migraine (73.1% vs. 50.7% , P cam was a friend or relativ e (52.7% ). In most cases, migraineurs’ recourse to cam treatments was specif ically for their headache (89.3% ). Approximately 61% of cam users had not in formed their medical doctors of their cam use. The most common reason for decidi ng to try a cam therapy was that it offered a ’ potential improvement of headac he’ (47.7% ). The greatest users of cam treatments were: patients with a diag nosis of transformed migraine; those who had consulted a high number of speciali sts and reported a higher lifetime number of conventional medical visits; those with a comorbid psychiatric disorder; those with a high income; and those whose headache had been either misdiagnosed or not diagnosed at all. Our findings sugg est that headache clinic migraine patients, in their need of and quest for care, seek and explore both conventional and cam approaches. Physicians should be mad e aware of this patient- driven change in the medical climate in order to preve nt misuse of healthcare resources and to be better equipped to meet patients’ needs.

关键词：偏头痛患者 cam 变异型执业医师医疗保健率比常规医疗