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作者： ALI ABDULLAH SALEH HZam ALT am（艾力）东华大学

学位级别：硕士

纤维素是世界上最丰富的天然聚合物,因此它被视为非常重要的多用途的原材料,基于其特殊的结构和功能性质,广泛应用于医疗产品。纤维素的结构中有羟基,能够部分的或者完全的与不同元素发生反应,得到的衍生物具有多功能性质,例如纤维素酯... 详细信息

纤维素是世界上最丰富的天然聚合物,因此它被视为非常重要的多用途的原材料,基于其特殊的结构和功能性质,广泛应用于医疗产品。纤维素的结构中有羟基,能够部分的或者完全的与不同元素发生反应,得到的衍生物具有多功能性质,例如纤维素酯和纤维素醚。纤维素衍生物在日常生活中被广泛应用,因此它必须无致癌性、无毒性、生物相容性并且对生物环境无排斥性。在食品和药物企业,纤维素醚是最广泛应用的纤维素衍生物,如羧甲基纤维素(CMC),阳离子纤维素(CC)。纤维素醚是通过用烷基或者羟烷基置换羟基得到的,取代基的化学性质,数量和分布影响着纤维素醚的溶解性、热稳定性、胶体性质、表面活性、热塑性、薄膜属性和生物降解性。镧系元素原子结构有4f轨道,使得它们表现出特殊的化学和光谱性质。镧系元素的麻醉剂吸引了人们的极大兴趣,因其在催化和发光领域的潜在应用。一些镧系离子,如铽和铕,已经广泛应用于原子聚集和生物分子结构的发冷光试验和染色试验中。理解高分子与金属离子间的相互作用对科学研究和创新实验具有重大意义。两者的相互作用力主要是静电力和配位作用力。金属很容易失去电子变成离子,同时自然界中有很多带负电荷的高分子,因此静电力相互作用较为常见。同时,金属离子的原子结构有空轨道,并且高分子上的基团能够提供电子对,以此形成配位键。有研究表明羧酸基团和金属离子的配位相互作用分为单齿,双齿,和三齿螯合。在过去几年,人们尝试了许多界面复合的方法来组装纤维素衍生物到聚电解质多层薄膜中,如基于静电作用为主要驱动力的层层组装技术,该技术是一个简单高效的制备薄膜的方法。层层组装薄膜有诸多应用,如光学过滤膜、离子交换膜、防腐涂层、化学和生物传感器以及药物传递层。层层组装技术也可以用来扩展纤维素的应用,但是纤维素不能直接用来制备薄膜,只有通过化学改性,纤维素才可以。最近,高分子螯合物已经被用于层层组装的基础材料。有一类的高分子螯合物是超高分子聚集体,它是基于不同的二级相互作用形成的,包含有高分子-高分子螯合物,高分子-金属螯合物和高分子-表面活性剂螯合物。高分子螯合物的层层组装使得它易于形成微米级的薄膜并且能引入更多的材料进入薄膜中,这对材料的多功能应用有很大意义,例如超疏水、自修复等。在我的研究体系,研究工作的主要目的是引入镧系离子进入纤维素衍生物薄膜中,先制备带负电荷的CMC-Ln的复合物纳米颗粒,然后与带正电荷的CC层层组装,通过这种方法,成功得到CMC-Ln-QC复合物薄膜。我们分别制备了pH=4和pH=6两个pH条件下的CMC-Ln-QC复合物薄膜.研究工作的第一部分是在pH4和pH6的条件下制备CMC-Ln的配合物。CMC包含很多羧酸根和羟基基团,能够基于配位作用和镧系离子形成聚合物金属复合物。在严格控制的pH值和质量百分比的条件下混合相同体积的CMC和Ln离子溶液制备CMC-Ln复合物纳米颗粒。在pH6,质量百分比4:1和pH4,质量百分比6:1的条件下,能得到分散的CMC-Ce复合物纳米颗粒;(i)直接混合Ce离子溶液和CMC溶液(M1),(ii)逐滴地滴加Ce离子溶液到CMC溶液中,滴加速率为1m L/h(M2),(iii)逐滴地滴加CMC溶液到Ce离子溶液中,滴加速率为1m L/h(M3)。我们使用动态光散射(DLS)测试了CMC-Ce复合物纳米颗粒的尺寸和分布,在pH4,M1,M2和M3的平均流体动力学直径分别是610nm,450 nm和740 nm。在pH6时,分别是650 nm,350 nm和1150 nm,同时使用紫外可见光谱UV-Vis证明了纳米颗粒中包含有铈离子。对于M1,M2,M3,CMC-Ce复合物纳米颗粒在pH4的Zeta电位分别是-21.2,-19.2,-20.6,在pH6分别是-43.7,-36.8,-38.6。随着pH值的增加,去离子化增强,得到更多的羧酸根离子。红外光谱FT-IR可用来表征CMC的去离子化程度。根据公式(1)的计算,在pH=2时,CMC的去离子化程度是15%,在pH=4是55%,在pH=6时接近100%。羧酸根离子COO-的对称和非对称伸缩振动峰的差值,可以反映CMC和铈离子的配位作用方式,不同的方式取决于组装的pH和质量百分比。研究工作的第二部分是利用两种带有相反电荷的物质制备薄膜。层层组装技术将负电荷的均匀分散的CMC-Ln配合物胶体颗粒和正电荷的阳离子纤维素交替沉积在石英片或者硅片上来制备薄膜。在pH4和pH6的不同条件下制备薄膜,并且薄膜的性质可通过不同的光谱技术来研究。反射光谱用来表征薄膜厚度,pH=4的条件下制备的[CC/(CMC-Ce)]薄膜非常浑浊,在光谱上没有指纹峰出现,厚度很难测试。在pH=6的条件下制备的薄膜比较透明,可用公式(2)计算厚度。在pH=6的M1和M2方法制备的薄膜厚度分别为1222 nm和750 n

关键词：纤维素纤维素衍生物羧甲基纤维素阳离子纤维素镧系离子层层组装

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Detection of influenza A virus RNA in birds by optimized Real-Time PCR system

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Asian Pacific Journal of Tropical Medicine 2010年第5期3卷 337-340页

作者： Ilinykh Ph A Shestopalova EM Khripko Yu I Durimanov Ag Sharshov KA Shestopalov am State Research Center of Virology and Biotechnology Vector RussiaNovosibirsk region630559 Koltsovo

Objective:To evaluate the use of Real-Time PCR system based on specific amplification of matrix protein gene fragment for influenza A virus RNA detection in cloacal swabs from wild ***:Sensitivity,specificity and reproducibility of analysis results were identified. Study of cloacal swabs from wild birds for influenza A virus presence was ***: Reproducibility of low concentrations of virus detection in samples by Real-Time PCR was significantly higher than that of detection based on cytopathic effect of viruses grown on MDCK cell ***:Real-Time PCR system for influenza A virus RNA detection is developed and applied for virus surveillance study.

关键词： Influenza A virus RNA Real-Time PCR MDCK cell culture Virus surveillance Wild birds

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不全流产的抗生素应用

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生殖与避孕 2005年第2期25卷 116-118页

作者： May W gülmezoglu am Ba-Thike K 刘付朝晖单位不详

1背景不安全流产是影响公众健康的全球性问题.世界卫生组织估计每年约有2千万例不安全流产,其中有10%至50%因不安全流产发生并发症而需要医治(WHO 1994).世界上约有13%的死亡是由与妊娠有关的不安全流产引起的,大多数的死亡(和发病)发... 详细信息

1背景不安全流产是影响公众健康的全球性问题.世界卫生组织估计每年约有2千万例不安全流产,其中有10%至50%因不安全流产发生并发症而需要医治(WHO 1994).世界上约有13%的死亡是由与妊娠有关的不安全流产引起的,大多数的死亡(和发病)发生在发展中国家,在有些国家中流产因法律而受限制.亚洲有20%-25%的孕产妇死亡,非洲和拉丁美洲有30%-50%的孕产妇死亡,俄罗斯有25%-30%的孕产妇死亡被认为是由人工流产引起的(Henshaw 1990,Popov 1991).

关键词：孕产妇死亡不全流产抗生素应用并发症发病 WHO 人工流产非洲限制

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Positron emission tomography to assess hypoxia and perfusion in lung cancer

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World Journal of Clinical Oncology 2014年第5期5卷 824-844页

作者： Eline E Verwer Ronald Boellaard Astrid am van der Veldt Department of Radiologyand Nuclear Medicine VU University Medical Center 1007 MBAmsterdam The Netherlands Department of Internal Medicine VUUniversity Medical Center 1007 MB Amsterdam The Netherlands

In lung cancer, tumor hypoxia is a characteristic feature, which is associated with a poor prognosis and resistance to both radiation therapy and chemotherapy. As the development of tumor hypoxia is associated with decreased perfusion, perfusion measurements provide more insight into the relation between hypoxia and perfusion in malignant tumors. Positron emission tomography(PET) is a highly sensitive nuclear imaging technique that is suited for non-invasive in vivo monitoring of dynamic processes including hypoxia and its associated parameter perfusion. The PET technique enables quantitative assessment of hypoxia and perfusion in tumors. To this end, consecutive PET scans can be performed in one scan session. Using different hypoxia tracers, PET imaging may provide insight into the prognostic significance of hypoxia and perfusion in lung cancer. In addition, PET studies may play an important role in various stages of personalized medicine, as these may help to select patients for specifictreatments including radiation therapy, hypoxia modifying therapies, and antiangiogenic strategies. In addition, specific PET tracers can be applied for monitoring therapy. The present review provides an overview of the clinical applications of PET to measure hypoxia and perfusion in lung cancer. Available PET tracers and their characteristics as well as the applications of combined hypoxia and perfusion PET imaging are discussed.

关键词： Molecular imaging Positron emission tomography Hypoxia Perfusion Quantification Lung cancer

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Bacterial and fungal endophthalmitis in Upper Egypt:related species and risk factors

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Asian Pacific Journal of Tropical Biomedicine 2012年第8期2卷 655-659页

作者： AA gharamah am Moharram MA Ismail AK AL-Hussaini School of Biological Sciences Universiti Sains Malaysia Department of Botany Faculty of SciencesAssiut University Department of Ophthalmology Faculty of MedicineAssiut University

Objective:To study risk factors,contributing factors of bacterial and fungal endophthalmitis in Upper Egypt,test the isolated species sensitive to some therapeutic agents,and to investigate the air-borne bacteria and fungi in opthalmology operating ***:Thirty one cases of endophthalmitis were clinically diagnosed and microbiologically *** air-borne bacteria and fungi inside four air-conditioned operating rooms in the Ophthalmology Department at Assiut University Hospitals were also *** isolated microbes from endophthalmitis cases were tested for their ability to produce some extracellular enzymes including protease,lipase,urease,phosphatase and *** the ability of 5 fungal isolates from endophthalmitis origin to produce mycotoxins and their sensitivity to some therapeutic agents were ***:Results showed that bacteria and fungi were responsible for infection in 10 and 6 cases of endophthalmitis,respectively and only 2 cases produced a mixture of bacteria and *** was the most prevalent risk factor of endophthalmitis where 58.1%of the 31 cases were due to *** ophthalmology operating rooms,different bacterial and fungal species were isolated.8 bacterial and 5 fungal isolates showed their ability to produce enzymes while only 3 fungal isolates were able to produce *** showed the highest effect against most isolates in ***:The ability of bacterial and fungal isolates to produce extracellular enzymes and mycotoxins may be aid in the invasion and destruction of eye tissues. Microbial contamination of operating rooms with air-borne bacteria and fungi in the present work may be a source of postoperative endophthalmitis.

关键词： Bacterial Fungal Endophthalmitis Upper Egypt Risk factors Trauma Ophthalmic surgery

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Acceptability of a task sharing and shifting model between family physicians and physiotherapists in French multidisciplinary primary healthcare centres:a cross-sectional survey

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Family Medicine and Community Health 2022年第2期10卷 11-19页

作者： amélie Kechichian François Desmeules Pauline girard Nicolas Pinsault Themas Team TIMC-IMAG LaboratoryUMR CNRS-UGAGrenobleFrance Department of Physiotherapy University Grenoble-AlpesGrenobleAuvergne-Rhône AlpesFrance Maisonneuve-Rosemont Hospital Research Centre University of Montreal Affiliated Research CenterMontrealQuébecCanada School of Rehabilitation University of MontrealFaculty of MedicineMontrealQuébecCanada Department of general Medicine Faculty of MedicineUniversity Grenoble-AlpesGrenobleFrance

Objectives The rising prevalence of musculoskeletal disorders increases pressure on primary care *** France,patients with musculoskeletal disorders are referred to physiotherapist(PT)by family physician(FP).To improve access to musculoskeletal care,a new model of task sharing and shifting is implemented between FPs and PTs for patients with acute low back *** new model enables French PTs to expand their usual scope of practice by receiving patients as first-contact practitioner,diagnosing low back pain,prescribing sick leave and analgesic *** aim of this study is to investigate the acceptability of FPs and PTs regarding this new *** A cross-sectional survey design was *** was measured using a questionnaire on the perception of the model and the perception of PTs’skills to manage low back *** analyses were performed to compare results among *** French FPs and PTs working in multidisciplinary primary healthcare centres were invited to complete an online *** A total of 174 respondents completed the survey(81 FPs and 85 PTs).Results A majority of participants had a positive perception of the task sharing and shifting model.A majority of the participants were mostly or totally favourable towards the implementation of the model(FPs:n=46,82%and PTs:n=40,82%).The perceived level of competencies of PTs to manage acute low back pain was *** confidence level of FPs was higher than that of PTs regarding PTs’ability to adequately diagnose low back pain,refer patient to physiotherapy and prescribe sick leave or analgesic *** Based on this limited sample of participants,there appears to be good acceptability of the task sharing and shifting model for acute low back *** studies are needed to better determine the factors affecting the acceptability of such a model.

关键词： shifting centre sectional

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Qualitative analysis of catechins from green tea gMB-4 clone using HPLC and LC-MS/MS

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Asian Pacific Journal of Tropical Biomedicine 2015年第12期5卷 1046-1050页

作者： Erna Susanti Ciptati Retty Ratnawati Aulanni'am Achmad Rudijanto Doctoral Program of Biomedical Science Faculty of Medicine Brawijaya University Department of Chemistry Faculty of Science Bandung Institute of Technology Biomedical Science Faculty of Medicine Brawijaya University

Objective:To identify the bioactive compounds in catechins isolation and its components from green tea gMB-4 ***:green tea gMB-4 clones were extracted with distilled water at *** were eluted into the column with 10%***,the column was eluted with95%ethanol and evaporated *** tea extract was identified by thin layer *** were separated by the stationary phase in column chromatography using polyamide with 10%ethanol eluent and 95%*** results of isolations were analyzed by high performance liquid chromatographic(HPLC)and LCMS/*** of catechins by HPLC was done by external ***:Fraction from 10%ethanol showed that four major peaks at retention time of1.663,2.367,2.950 and 4.890,indicated the presence of four catechins components including catechin,epicatechins,gallocatechin and ***,fraction from 95%ethanol showed two main peaks at retention time of 5.167 and 9.82,which indicated the presence of epigallocatechin gallate(EgCg)and epicatechin gallate(ECg).EgCg(m/z 459),epigallocatechin(m/z 307),ECg(m/z 443),and epicatechin(m/z 291)were isolated and separated successfully using HPLC and LC-MS/***:The HPLC and LC-MS/MS methods were successfully tuned for the qualitative analysis of green tea extract with EgCg and *** major catechins were separated and identified by LC-MS/MS,such as EgCg,epigallocatechin,ECg and *** result of HPLC analysis showed that EgCg and ECg were main components from catechins isolation of green tea gMB-4 clone.

关键词： Catechins green tea gMB-4 clone LC-MS/MS HPLC

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Role of CARD15,DLg5 and OCTN genes polymorphisms in children with inflammatory bowel diseases

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World Journal of gastroenterology 2007年第8期13卷 1221-1229页

作者： S Cucchiara A Latiano O Palmieri am Staiano R D'Incà g guariso g Vieni V Rutigliano O Borrelli MR Valvano V Annese Clinica Pediatrica Università "La Sapienza" Roma Italy U.O. gastroenterologia IRCCS "Casa Sollievo della Sofferenza" San Giovanni Rotondo Italy Clinica Pediatrica Università di Napoli Università di Padova Italy Cattedra di gastroenterologia Università di Padova Italy Clinica Pediatrica Università di Padova Italy Clinica Pediatrica Università di Messina Italy Clinica Pediatrica Università di Bari Italy

AIM: To investigate the contribution of variants of CARD15, OCTN1/2 and DLg5 genes in disease predispo- sition and phenotypes in a large Italian cohort of pediatric patients with inflammatory bowel diseases (IBD). METHODS: Two hundred patients with Crohn’s disease (CD), 186 ulcerative colitis (UC) patients, 434 par- ents (217 trios), and 347 healthy controls (HC) were studied. Polymorphisms of the three major variants of CARD15, 1672C/T and -207g/C SNPs for OCTN genes, IgR2096a_1 and IgR2198a_1 SNPs for the IBD5 locus, and 113g/A variant of the DLg5 gene were evaluated. Potential correlations with clinical sub-phenotypes were investigated. RESULTS: Polymorphisms of CARD15 were significantly associated with CD, and at least one variant was found in 38% of patients (15% in HC, OR = 2.7, P gosis for both OCTN1/2 variants was more com- mon in CD patients (1672TT 24%, -207CC 29%) than in HC (16% and 21%, respectively; P = 0.03), with an in- creased frequency of the TC haplotype (44.8% vs 38.3% in HC, P = 0.04). No association with the DLg5 variant was found. CD carriers of OCTN1/2 and DLg5 variants more frequently had penetrating disease (P = 0.04 and P = 0.01), while carriers of CARD15 more frequently had ileal localization (P = 0.03). No gene-gene interaction was found. In UC patients, the TC haplotype was morefrequent (45.4%, P = 0.03), but no genotype/phenotype correlation was observed. CONCLUSION: Polymorphisms of CARD15 and OCTN genes, but not DLg5 are associated with pediatric on- set of CD. Polymorphisms of CARD15, OCTN, and DLg5 genes exert a weak influence on CD phenotype.

关键词： Inflammatory bowel disease Ulcerative colitis Crohn's disease CARD15 DLg5, Carnitine/organic cation transporter gene

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Side－effects　of　organic　and　inorganic　pollutants　on　soil　nitrification　and　respiration

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Journal of Environmental Sciences 1996年第1期8卷 66-76页

作者： gong Ping Sun Tieheng (Institute of Applied Ecology,Chinese Academy of Sciences, Shenyang 110015, China )g nter Beudert Hermann. H. Hahn (Institut fur Siedlungswasserwirtschaft,Der Universitat Karlsruhe).(Postfach 6980,am Fasanengarten,D-76128 Karlsruhe China

Side-effects of heavy metals(Cd and Zn)and organic pollutants(phenanthrene and paclobutrazol)on nitrification and respiration in a loam soil were investigated with its air-dried and fresh conditions. Critical levels for these test pollutants were derived from this *** concentrations of Cd and Zn were also determined. It was concluded that such critical levels as NOEL and LOEL of heavy metals should be expressed using bioavailable concentrations rather than total ones since different soil has different properties,especially different absorption ability to heavy metals.

关键词： nitrification respiration organic pollutants heavy metal bioavailable concentration.

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Phosphorylation of Izumo1 and its role in male nfertility

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Asian Journal of Andrology 2015年第5期17卷 708-710,I0005页

作者： Samantha am Young John Aitken Mark A Baker priority Research Centre in Reproductive Science Discipline of Biological Sciences Faculty of Science and IT University of Newcastle Callaghan NSW Australia Animal Resource Centre for Infectious Diseases Research Institute for Microbial Diseases Osaka University Suita Osaka Japan

Izumol is a testis-specific gene product, whose function is essential for sperm-egg fusion. Throughout its lifespan, Izumol is posttranslationally modified, being both N-linked glycosylated on its extracellular domain and phosphorylated on the intracellular C-terminal tail. Within the caput regions of the rat epididymis, two phosphorylation events have been documented. However, as sperm pass through the epididymis, this cytoplasmic portion of Izumol has been shown to contain up to seven phosphorylation sites. Remarkably, in the rat, in correlation with these events, Izumol undergoes sub-cellular re-location, moving from the head/tail regions of the spermatozoa, to a predominantly equatorial segment location once they have reached the caudal end of the epididymis.

关键词： epididymis Izumol LC-MS phosphorylation proteomics

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