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CRIsPR/Cas9-mediated knockout of E4 gene promotes maturation in soybean

Oil Crop science 2024年第3期9卷 170-176页

作者： Wu s. Chen l. guo M. Cai Y. gao Y. Yuan s. sun s. Zhang Y. Hou W. Han T. College of Agriculture Heilongjiang Bayi Agricultural University Daqing 163316 China Ministry of Agriculture Key laboratory of soybean Biology (Beijing) Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China College of Agriculture Northeast Agricultural University Harbin 150030 China state Key laboratory of Crop germplasm Innovation and Molecular Breeding Syngenta Biotechnology (China) Co. Ltd Beijing 100081 China

soybean is a broadly popular and extensively cultivated crop, however, many high-yield and high-quality varieties require specific growth conditions, restricting their widespread adoption. The appropriate light conditions and photoperiod must be attained for these varieties to thrive in new environments. In this study, we employed CRIsPR/Cas9 to design two sgRNAs aimed at knocking out the maturity-related gene E4 in a major American soybean variety called ''Jack'', which belongs to maturity group MgII. E4 gene is primarily involved in the photoperiodic flowering and maturity in soybean, making it an ideal candidate for genetic manipulation. We successfully obtained 1 homozygous E4-sg1 mutant type with 1-bp insertion, and 4 homozygous E4-sg2 mutants type with 2-bp deletion, 7-bp deletion, 61-bp deletion, and 1-bp insertion, respectively. The homozygous e4 mutant plants contained early termination codons devoid of transgenic elements. Additionally, no potential off-target sites of the E4 gene were detected. A comparative analysis revealed that, unlike the wild-type, the maturity time of homozygous e4 mutants was early under both short-day and long-day conditions. These mutants offer novel germplasm resources that may be used to modify the photoperiod sensitivity and maturity of soybean, enhancing its adaptability to high-latitude regions. © 2024 the Oil Crop Research Institute, Chinese Academy of Agriculture sciences

关键词： CRIsPR/Cas9 E4 Maturity soybean

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Effect of Ta_(2)O_(5)nanoparticles on bioactivity,composition,structure,in vitro and in vivo behavior of PEO coatings on Mg-alloy

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Journal of Magnesium and Alloys 2024年第6期12卷 2360-2379页

作者： D.V.Mashtalyar I.M.Imshinetskiy V.V.Kashepa K.V.Nadaraia M.A.Piatkova A.I.Pleshkova K.A.Fomenko A.Yu.Ustinov s.l.sinebryukhov s.V.gnedenkov Institute of Chemistry Far Eastern BranchRussian Academy of SciencesVladivostok690022Russia Far Eastern Federal University Vladivostok690950Russia Regional Clinical Dermatovenerological Dispensary Vladivostok690033Russia

The present study investigates the physical and chemical characteristics,behavior in vitro and in vivo,and biocompatibility of coatings containing Ta_(2)O_(5),which are obtained by plasma electrolytic oxidation(PEO)on MA8 magnesium *** obtained coatings demonstrate in vivo biocompatibility and in vitro *** to the base PEO coating,the layers containing Ta_(2)O_(5)facilitate the development of apatite in simulated body fluid,suggesting that the inclusion of nanoparticles improves bioactivity of the *** was found that incorporation of Ta_(2)O_(5)nanoparticles increases roughness and porosity of the formed layers by increasing particle concentration in electrolytes for the PEO process contributing to sufficient soft tissue ingrowth in *** on in vivo studies,these coatings also provide favorable tissue response and minimal inflammatory reaction in comparison with the bare magnesium alloy due to protection of living tissues from deleterious corrosion events of magnesium implant such as local alkalization and intense hydrogen *** results obtained in the present study concluded biocompatibility,tissue integration of the PEO coatings containing Ta_(2)O_(5)nanoparticles making them a promising protective layer for biodegradable magnesium implants.

关键词： Plasma electrolytic oxidation Bioactive coatings Tantalum pentoxide simulated body fluid(sBF) Apatite formation Histology

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Neuroimaging in atypical normal tension glaucoma:debating routine implementation in the absence of classic neurological findings

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 509-517页

作者： Umay güvenç gülizar Demirok güner Üney selma Uzman Ophthalmology Department Ankara Training and Research HospitalAnkara 06230Turkey

●AIM:To assess the necessity of neuroimaging in patients with neurological or atypical findings of normal tension glaucoma(NTg)who do not exhibit typical glaucoma manifestations.●METHODs:A retrospective analysis was conducted on 90 atypical NTg patients who underwent cranial magnetic resonance imaging(MRI)due to atypical *** demographic characteristics,clinical parameters,and radiological findings were recorded.●REsUlTs:Among the patients,66.7%had abnormal radiology results,with the most common findings being gliosis(34.4%),sequelae of cerebrovascular events and vascular malformations(14.4%),and benign intracranial mass lesions(11%).Non-glaucomatous visual field defects were more frequently observed in patients with abnormal neuroimaging ***,there were no significant differences in intraocular pressure,optic disc parameters,retinal nerve fiber layer thickness,and visual field indices between patients with normal and abnormal radiological *** mean age of the patients was ***,there was a significant age difference,with the abnormal radiology group having a higher median age(P=0.021).●CONClUsION:The study highlights the importance of cranial imaging in older NTg patients to detect underlying pathologies and prevent *** suggests that neuroimaging may be warranted in NTg patients with atypical visual field defects incompatible with ***,routine neuroimaging in all NTg patients without classic neurological signs may not be necessary.

关键词： normal tension glaucoma neuroimaging atypical visual field defects

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Determining the Checkpoint of Transition from Thin to Thick Periodontal Phenotypes in the Maxillary and Mandibular Anterior Regions of south Indian Population

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Journal of Forensic science and Medicine 2024年第1期10卷 55-61页

作者： Mohan Kumar Pasupuleti Rasagna Chowdary g gautami sPenmetsa Vivek Bypalli Ramesh s.V.Konathala sruthima N.V.s.gottumukkala Department of Periodontics and Implantology Vishnu Dental CollegeBhimavaramAndhra PradeshIndia2Vishnu Dental CollegeBhimavaramAndhra PradeshIndia

Aim:The aim of this study was to compare and investigate the tissue thickness at the maxillary and mandibular central incisors utilizing the probe visibility method and traditional way of measuring gingival thickness(gT)by endodontic ***:In 200 selected patients,the gT was measured at both the maxillary and mandibular anterior using an endodontic file after local infiltration at the mid-distance from the gingival margin to the mucogingival *** checkpoint of transition from thin to thick periodontal phenotype was determined by probe visibility method in the following age groups:group A:21-30 years;group B:31-40 years;group C:41-50 years;and group D:51-60 ***:gT varied from 0.3 to 1.9 *** translucency was observed up to the thickness of 0.7 *** 0.8 mm of gT,there was no probe ***:According to the present research,the gingiva appears transparent up to a thickness of around 0.7 *** checkpoint of transition between thin and thick periodontal phenotypes at both the maxillary and mandibular anterior was associated with a gT of 0.8 mm.

关键词： gingiva periodontal phenotype probe visibility

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Effect of Eu3+,Bi3+,and li+ doping on luminescent property of gdNbO4

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Journal of Rare Earths 2024年第1期42卷 66-75页

作者： s.W.Wi Y.s.lee Department of Physics and Integrative Institute of Basic sciences Soongsil University

We investigated the emission properties of Eu3+-doped gdNbO4-based oxide phosphors,aiming at improving the Eu3+emission by codoping of Bi3+and li+.Eu3+singly doped,Eu3+and Bi3+doubly doped,and Eu3+,Bi3+,and li+triply doped gdNbO4were prepared using a high-temperature solid-state reaction *** red-orange emissions from Eu3+in the singly doped samples are significantly improved by the codoping of Bi3+,mainly due to the energy transfer from Bi3+to Eu3+.The additional codoping of li+is found to increase the Eu3+emission *** improvement might be attributed to the increase in the photoluminescent quantum yield originating from larger grain sizes and better crystallinity.A detailed analysis of the X-ray diffraction pattern and the asymmetric ratio estimated from the photoluminescence spectra show that the local lattice environment around Eu3+in gdNbO4does not change significantly with the codoping of Bi3+and li+.Our results indicate that li+doping is a promising way to improve the emission properties of rare-earth ion-doped gdNbO4phosphors.

关键词： Eu 3+ gdNbO 4 Codoping of li + Photoluminescence sensitizer Rare earths

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Prediction of Dynamic and Aerodynamic Characteristics of the Centrifugal Fan with Forward Curved Blades

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Journal of Thermal science 2013年第6期22卷 517-521页

作者： Jií Polansky lászló Kalmár Roman gápár Univerzitní 8 306 14 PlzeňCzech Republic Miskolc-Egyetemváros MiskolcHungary

The main aim of this paper is determine the centrifugal fan with forward curved blades aerodynamic characteristics based on numerical modeling. Three variants of geometry were investigated. The first, basic "A" variant contains 12 blades. The geometry of second "B" variant contains 12 blades and 12 semi-blades with optimal length[1]. The third, control variant "C" contains 24 blades without semi-blades. Numerical calculations were performed by CFD Ansys. Another aim of this paper is to compare results of the numerical simulation with results of approximate numerical procedure. Applied approximate numerical procedure [2] is designated to determine characteristics of the turbulent flow in the bladed space of a centrifugal-flow fan impeller. This numerical method is an extension of the hydro-dynamical cascade theory for incompressible and inviscid fluid flow. Paper also partially compares results from the numerical simulation and results from the experimental investigation. Acoustic phenomena observed during experiment, during numerical simulation manifested as deterioration of the calculation stability, residuals oscillation and thus also as a flow field oscillation. Pressure pulsations are evaluated by using frequency analysis for each variant and working condition.

关键词： Centrifugal Fan CFD Pressure Coefficient Flow Coefficient

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Cataract blindness in Hungary

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International Journal of Ophthalmology(English edition) 2020年第3期13卷 438-444页

作者： gábor l.sándor gábor Tóth Dorottya szabó Irén szalai Regina lukács Anita Pék georgina Z.Tóth András Papp Zoltán Z.Nagy Hans limburg János Németh Department of Ophthalmology Semmelweis UniversityBudapest 1085Hungary Department of Ophthalmology Flór Ferenc HospitalKistarcsa 2143Hungary Department of Ophthalmology Petz Aladár HospitalGyor 9024Hungary Faculty of Health sciences Semmelweis UniversityBudapest 1088Hungary Health Information services Grootebroek 1613The Netherlands

AIM:To estimate the prevalence of blindness and visual impairment resulting from cataract in the population aged≥50 y in Hungary,and to assess the cataract surgical ***:A rapid assessment of avoidable blindness(RAAB)was conducted.A total of 3523 eligible people were randomly selected and *** participant underwent surgery for cataract was interviewed with regard to the year,place,and costs of the *** with obvious cataract were asked why they had not yet undergone surgery(barriers to surgery).REsUlTs:An estimated 12514 people were bilaterally blind;the visual acuity(VA)in 19293 people wasle wasstimated 77933 eyes are blind;98067 eyes had a VA ofstimated 277493 eyes had a VA ofll cataract surgeries were conducted in government *** age-and sexadjusted cataract surgical coverage with VAs was 90.0%.The rate of good visual outcome after surgery was 79.5%.Ocular comorbidity was the main cause of poor outcome(78.1%),followed by late complications(such as posterior capsule opacification)(17.2%),inadequate optical correction(3.1%),and surgical complications(1.6%).The main barrier to surgery in people with bilateral cataract and VA ofs‘need not felt’.CONClUsION:The prevalence of visual impairment resulting from cataract is slightly higher than *** quality of the cataract surgical service seems adequate in ***,the number of cataract operations per year should continue to increase due to the increasing patient demands and the aging population.

关键词： cataract prevalence blindness visual impairment rapid assessment of avoidable blindness

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solid-liquid extraction for yttrium recovery using porous polymeric resin(XAD-7) functionalized with D_(2)EHPA

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Journal of Rare Earths 2024年第9期42卷 1764-1773,I0005页

作者： P.A.Martínez-Montoya R.g.sanchez-Alvarado D.Y.Medina-Velazquez M.l.Carrera-Jota P.garnica-Chávez A.de J.Morales-Ramirez Higher school of Chemical Engineering and Extractive Industries ESIQIEIPNDepartment of Metallurgical EngineeringUPALM S/NCol.LindavistaGustavo A.Madero Cd.de MéxicoC.P07738Mexico Metropolitan Autonomous University Department of MaterialsBasic Sciences and EngineeringAv.San Pablo No.180Col.Reynosa TamaulipasCd.de MéxicoC.P02200Mexico Center for Research and Technological Innovation CIITECIPNCda.de CECATIs/nSanta CatarinaAzcapotzalcoC.P02250CDMXMexico

The recovery of yttrium is proposed by applying a solid-liquid extraction process using di-2-ethyl hexylphosphoric acid(D2EHPA) as extracting *** extracting agents were supported on a macro porous polymeric resin XAD-7(solid phase).Yttrium ions extraction and discharge tests were performed,firstly from a synthetic aqueous solution of 100 mg/l Y(liquid phase) at 25℃ with *** effects of pH of aqueous solutions bearing yttrium,volume fraction of extracting agents and the solid/liquid(s/l)ratio on the yttrium recovery were *** most favorable conditions for yttrium ions extraction are;20 vol% D_(2)EHPA functionalized resin,pH=1.5 and an s/l ratio of 10 mg/ml The discharge of yttrium ions was done under the same conditions of extraction stage,using a 2 mol/l [H_(2)sO_(4)] as stripping *** to 80% yttrium is extracted,while 75% yttrium is recovered in the striping *** all experiments,the reaction equilibrium is reached after 20 min,and the kinetics for the extraction stage was determined as a second-order ***,experiments were carried out to discharge the yttriumloaded resins,and it has been determined that the best pH value to strip the Y ions is *** tests of extraction and discharge for yttrium ions show that the functionalized resin can work at least five cycles without decreasing its ***,the proposed process was tested in a real solution with Y ions fro m a waste fluorescent la mp powder leached in H_(2)sO_(4),demonstrating the ability to effectively recover yttrium,separating it from various metals from the studied residue.

关键词： Yttrium recovery Rare earth solid-liquid extraction XAD-7 resin Fluorescent lamps recycling solid-liquid kinetics extraction

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Further investigation on the fusion of ^(6)li with ^(209)Bi target at near-barrier energies

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Chinese Physics C 2024年第7期48卷 106-117页

作者：张高龙焦振威张广鑫 M.R.Cortes 胡世鹏钱建强 D.Mengoni 屈卫卫李聪博郑云张焕乔孙慧斌王楠张春雷 J.J.Valiente-Dobón D.Testov M.Mazzocco A.gozzelino C.Parascandolo D.Pierroutsakou M.la Commara A.goasduff D.Bazzacco D.R.Napoli F.galtarossa F.Recchia A.Illana s.Bakes I.Zanon s.Aydin g.de Angelis M.siciliano R.Menegazzo s.M.lenzi J.l.Ferreira J.Rangel serkan Akkoyun l.F.Canto J.lubian school of Physics Beihang UniversityBeijing 100191China sino-French Institute of Nuclear Engineering and Technology Sun Yat-Sen UniversityZhuhai 519082China Instituto de Física Universidade Federal Fluminense24210-340NiteróiRio de JaneiroBrazil Institute for Advanced study in Nuclear Energy&safety Shenzhen UniversityShenzhen 518060China shenzhen Key laboratory of Research and Manufacture of High Purity germanium Materials and Fetectors Shenzhen UniversityShenzhen 518060China INFN Sezione di PadovaPadovaItaly Dipartimento di fisica Astronomia dell'Universita di padova PadovaItaly state Key laboratory of Radiation Medicine and Protection School of Radiation Medicine and ProtectionSoochow UniversitySuzhou 215123China China Institute of Atomic Energy Beijing 102413China The Key laboratory of Beam Technology and Material Modification of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China INFN Laboratori Nazionali di LegnaroI-35020 LegnaroItaly INFN Sezione di NapoliI-80126 NapoliItaly Dipartimento di Farmacia Universitàdi Napoli"Federico II"I-80131 NapoliItaly Department of Natural and Mathematical sciences Faculty of EngineeringTarsus University33480 MersinTurkiye Physics Division Argonne National LaboratoryLemont-ILUSA Departamento de Matemaética Física e Computaç˜ao Universidade do Estado do Rio de JaneiroFaculdade de Tecnologia27537-000ResendeRio de JaneiroBrazil Department of Physics Sivas Cumhuriyet UniversitySivasTurkey Instituto de Física Universidade Federal do Rio de JaneiroCP 6852821941-972Rio de JaneiroBrazil

The complete and incomplete fusion cross sections for ^(6)li+^(209)Bi were measured using the in-beamγ-ray method around the Coulomb *** cross sections of(deuteron captured)incomplete fusion(ICF)products were re-quantified experimentally for this reaction *** results reveal that the ICF cross section is equivalent to that of complete fusion(CF)above the Coulomb barrier and dominant near or below the Coulomb barrier.A theoretical calculation based on the continuum discretized coupled channel(CDCC)method was performed for the aforementioned CF and ICF cross sections;the result is consistent with the *** universal fusion function(UFF)was also compared with the measured CF cross section for different barrier parameters,demonstrating that the CF suppression factor is significantly influenced by the choice of potential,which can reflect both dynamic and static effects of breakup on the fusion process.

关键词： cross section incomplete fusion suppression factor weakly bound nuclei

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The magnetosheath at high spectral resolution

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Earth and Planetary Physics 2024年第1期8卷 234-246页

作者： K.D.Küntz D.Koutroumpa W.R.Dunn A.Foster F.s.Porter D.g.sibeck B.Walsh The Henry A.Rowland Department of Physics and Astronomy Johns Hopkins University3701 San Martin DriveBaltimoreMD21218USA NAsA goddard space Flight Center 8800 Greenbelt RoadGreenbeltMD 20771USA lATMOs-OVsQ CNRSUVSQ Paris-SaclaySorbonne Université11 Boulevard d’Alembert78280GuyancourtFrance Department of Physics and Astronomy University College LondonLondonUK The Centre for Planetary science at UCl/Birkbeck Gower StreetLondon WC1E 6BTUK Center for Astrophysics Harvard University&Smithsonian Institution60 Garden StreetCambridgeMA 02138USA Center for space Physics Boston University110 Cummington MallBostonMAUSA

While we eagerly anticipate sMIlE’s(solar wind Magnetosphere Ionosphere link Explorer)unprecedented X-ray observations of the Earth’s magnetosheath and the initiation of a new era of magnetospheric research,it seems appropriate to look ahead to the abilities of the next generation of astrophysics *** these,the line Emission Mapper(lEM),a large aperture micro-calorimeter based mission,is currently planned to be able to observe the magnetosheath at high spectral resolution(~2 eV).With a field of view of~30′,lEM will allow higher spatial resolution and higher cadence measurement of the motion of a very small portion of the magnetopause over relatively short periods of time(multiple hours),complementing sMIlE’s global ***’s strength is its spectral *** will be able to measure the abundance of a broad range of elements and ionization states,many of which are inaccessible to current in situ instruments,and will be able to separate the emission from the magnetosheath from the emission from the cosmic X-ray background using the difference in their relative velocities.

关键词： magnetosheath charge exchange solar wind abundances

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