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The Crop Journal 2019年第4期7卷 494-503页

作者： fuying ma Xiaoyan Zhu Hui Wang Shiming Wang Guoqing Cui Ting Zhang Zhenglin Yang Guanghua He Yinghua Ling Nan Wang Fangming Zhao Rice Research Institute Academy of Agricultural Sciences Southwest University

A chromosome segment substitution line (CSSL) is a powerful tool for combining quantitative trait locus (QTL) mapping with the pyramiding of desirable alleles. The rice CSSL Z1364 with increased kernel number was identified in a BC3F8 population derived from a cross of Nipponbare as the recipient with Xihui 18 as the donor parent. Z1364 carried three substitution segments distributed on chromosomes 1, 6, and 8. The mean substitution length was 1.19 Mb. Of 17 QTL identified on the substitution segments, qSP1 for spikelets per panicle, qSSD1 for seed-set density, and qNSB1 for number of secondary branches explained respectively 57.34%, 87.7%, and 49.44% of the corresponding phenotypic variance and were all linked to RM6777. Chi-square analysis showed that the increased kernel number in Z1364 was inherited recessively by a single gene. By fine mapping, qSP1 was delimited to a 50-kb region on the short arm of chromosome 1. Based on DNA sequence, a previously uncharacterized rice homolog of Arabidopsis thaliana AT4G32551 was identified as a candidate gene for qSP1 in which mutation increases the number of spikelets and kernels in Z1364. qSP1 was expressed in all tissues, but particularly in 1-cm panicles. The expression levels of OsmaDS22, GN1A, and DST were upregulated and those of LAX2, GNP1, and GHD7 were downregulated in Nipponbare. These results provide a foundation for functional research on qSP1.

关键词： Rice Chromosome segment substitution line Increased number of kernels qSP1 QTL mapping for yield traits

Multi-genome evolutionary study of the ABC1 gene family and identification of the pleiotropic effects of OsABC1-13 in rice development

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The Crop Journal 2024年第4期12卷 1022-1030页

作者： fuying ma Mingyu Liu Peiwen Yan Shicong He Jian Hu Xinwei Zhang Fuan Niu Jinhao Cui Xinyu Yuan Xiaoyun Xin Liming Cao Jinshui Yang Ying Wang Xiaojin Luo State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology(Ministry of Education) School of Life SciencesFudan UniversityShanghai 200433China Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Department of Ecology and Evolutionary BiologySchool of Life SciencesFudan UniversityShanghai 200433China Institute of Crop Breeding and Cultivation Shanghai Academy of Agricultural SciencesShanghai 200241China Ministry of Education Key Laboratory of Crop PhysiologyEcology and Genetic Breeding College of AgronomyJiangxi Agricultural UniversityNanchang 330045JiangxiChina

In four rice genomes,85 ABC1-family genes were identified by comparative genomics,evolution,genetics,and ***,OsABC1-13,was shown by knockdown and knockout experiments to affect plant height,grain size,and photosynthet... 详细信息

关键词： Multi-genome analysis Activity of bc1 complex gene Photosynthesis Biomass Osabc1-13 Haplotype

The kinase OsSK41/OsGSK5 negatively regulates amylose content in rice endosperm by affecting the interaction between OsEBP89 and OsBP5

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Journal of Integrative Plant Biology 2023年第7期65卷 1782-1793页

作者： Zejun Hu Fuan Niu Peiwen Yan Kai Wang Lixia Zhang Ying Yan Yu Zhu Shiqing Dong fuying ma Dengyong Lan Siwen Liu Xiaoyun Xin Ying Wang Jinshui Yang Liming Cao Shujun Wu Xiaojin Luo Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops(Co‐construction by Ministry and Province) Ministry of Agriculture and Rural AffairsCrop Breeding and Cultivation Research InstituteShanghai Academy of Agricultural SciencesShanghai 201403China State Key Laboratory of Genetic Engineering and MOE Engineering Research Center of Gene Technology School of Life SciencesFudan UniversityShanghai 200438China MOE Key Laboratory of Crop Physiology Ecology and Genetic Breeding College of AgronomyJiangxi Agricultural UniversityNanchang 330045China

Amylose content(AC) is the main factor determining the palatability, viscosity, transparency, and digestibility of rice(Oryza sativa)grains. AC in rice grains is mainly controlled by different alleles of the Waxy(Wx) gene. The AP2/EREBP transcription factor OsEBP89 interacts with the MYC-like protein OsBP5 to synergistically regulate the expression of ***, we determined that the GLYCOGEN SYNTHASE KINASE 5(OsGSK5, also named SHAGGY-like kinase 41 [OsSK41]) inhibits the transcriptional activation activity of OsEBP89 in rice grains during amylose biosynthesis. The loss of OsSK41 function enhanced Wx expression and increased AC in rice grains. By contrast, the loss of function of OsEBP89 reduced Wx expression and decreased AC in rice grains. OsSK41 interacts with OsEBP89 and phosphorylates four of its sites(Thr-28,Thr-30, Ser-238, and Thr-257), which makes OsEBP89 unstable and attenuates its interaction with OsBP5. Wx promoter activity was relatively weak when regulated by the phosphomimicvariantOsEBP89E–OsBP5but relatively strong when regulated by the nonphosphorylatable variant OsEBP89A–***, OsSK41-mediated phosphorylation of OsEBP89 represents an additional layer of complexity in the regulation of amylose biosynthesis during rice grain development. In addition, our findings provide four possible sites for regulating rice grain AC via precise gene editing.

关键词： AP2/EREBP transcription factor endosperm amylose content GSK3-like family protein MYC-like protein OsSK41/OsGSK5 OsEBP89 OsBP5 rice grain

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谁在“简单化、误读和随意发挥”

全球教育展望 2006年第6期35卷 73-75页

作者：马福迎河南省焦作市马村区教育局教研室

时下进行的我国第八次课程改革,正处在由点到面的攻艰阶段。《中国教育报》2005年11月28日教育科学版刊登《谨防简单化、误读和随意发挥》一文,对我国新课程理论基础的评论不够客观。笔者作为一个基层教育工作者,仰望教育理论星光灿烂... 详细信息

时下进行的我国第八次课程改革,正处在由点到面的攻艰阶段。《中国教育报》2005年11月28日教育科学版刊登《谨防简单化、误读和随意发挥》一文,对我国新课程理论基础的评论不够客观。笔者作为一个基层教育工作者,仰望教育理论星光灿烂的天穹,对该文谈了一些不同看法。期望我国课程改革排除各种干扰,坚持正确的理论导向。

关键词：概念重建教条主义简单化课程改革理论基础多元智能理论建构主义

Auxin signaling module OsSK41-OsIAA10-OsARF regulates grain yield traits in rice

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Journal of Integrative Plant Biology 2023年第7期65卷 1753-1766页

作者： fuying ma Fan Zhang Yu Zhu Dengyong Lan Peiwen Yan Ying Wang Zejun Hu Xinwei Zhang Jian Hu Fuan Niu Mingyu Liu Shicong He Jinhao Cui Xinyu Yuan Ying Yan Shujun Wu Liming Cao Hongwu Bian Jinshui Yang Zhikang Li Xiaojin Luo State Key Laboratory of Genetic Engineering and MOE Engineering Research Center of Gene Technology School of Life SciencesFudan UniversityShanghai 200438China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China College of Agronomy Anhui Agricultural UniversityHefei 230036China Institute of Crop Breeding and Cultivation Shanghai Academy of Agricultural SciencesShanghai 201403China Institute of Genetics and Regenerative Biology Key Laboratory for Cell and Gene Engineering of Zhejiang ProvinceCollege of Life SciencesZhejiang UniversityHangzhou 310058China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518100China

Auxin is an important phytohormone in plants, and auxin signaling pathways in rice play key roles in regulating its growth, development, and productivity. To investigate how rice grain yield traits are regulated by auxin signaling pathways and to facilitate their application in rice improvement, we validated the functional relationships among regulatory genes such as OsIAA10, OsSK41, and OsARF21 that are involved in one of the auxin(OsIAA10) signaling pathways. We assessed the phenotypic effects of these genes on several grain yield traits across two environments using knockout and/or overexpression transgenic *** on the results, we constructed a model that showed how grain yield traits were regulated by OsIAA10 and OsTIR1, OsAFB2, and OsSK41 and OsmiR393 in the OsSK41-OsIAA10-OsARF module and by OsARF21 in the transcriptional regulation of downstream auxin response genes in the OsSK41-OsIAA10-OsARF module. The population genomic analyses revealed rich genetic diversity and the presence of major functional alleles at most of these loci in rice populations. The strong differentiation of many major alleles between Xian/indica and Geng/japonica subspecies and/or among modern varieties and landraces suggested that they contributed to improved productivity during evolution and breeding. We identified several important aspects associated with the genetic and molecular bases of rice grain and yield traits that were regulated by auxin signaling *** also suggested rice auxin response factor(OsARF) activators as candidate target genes for improving specific target traits by overexpression and/or editing subspecies-specific alleles and by searching and pyramiding the ‘best' gene allelic combinations at multiple regulatory genes in auxin signaling pathways in rice breeding programs.

关键词： allele evolution auxin signaling pathways OsARFs OsIAA10 yield traits

Mutation breeding inγ-linolenic acid improvement of Cunninghamella echinulata by Pulsed High magnetic Field

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Mutation breeding inγ-linolenic acid improvement of Cunning...

从边民到国民:彝族土司对彝族民众增强现代国民意识的历史贡献——以凉山彝族末代土司岭光电为个案

2013年湖北省暨武汉微生物学会会员代表大会暨学术年会

作者： Adnan.B.Al Hawash Xuejiao Zhang Shue Li Li Tan fuying ma Xiaoyu Zhang Key Laboratory of Molecular Biophysics of MOECollege of Life Science and Technology Huazhong University of Science and Technology

Spore suspension of an oleaginous fungus Cunninghamella echinulata was exposed under different intensity of pulsed high magnetic field for different times. After mutant,spore suspension was spread on PDA plate.64 mutants were isolated and screened by nile red staining and fluorescence microscopic examination.3 mutants were achieved for further fermentation *** biomass content,oil content andγ-linolenic acid(GLA) content of three mutants were higher than that of the *** GLA content was improved by 34.4%.So,pulsed high magnetic field mutation is an effective method for oleaginous fungi breeding.

关键词： Pulsed high-intensity magnetic field nile red staining γ-linolenic acid Cunninghamella echinulata

来源： cnki会议评论

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民族学刊 2023年第6期14卷 79-87,148页

作者：马富英依乌西南民族大学民族学与社会学学院四川成都610041 西南民族大学中国语言文学学院四川成都610225

在现代民族国家话语中,彝族没有以明确的国民身份出现在国家视野,而是作为“边民”散布于川康边地。文化隔阂与政治意识淡薄成为彝族民众增强现代国民意识的主要障碍。凉山彝族末代土司岭光电在彝区推行了一系列社会改革,其主要内容是... 详细信息

在现代民族国家话语中,彝族没有以明确的国民身份出现在国家视野,而是作为“边民”散布于川康边地。文化隔阂与政治意识淡薄成为彝族民众增强现代国民意识的主要障碍。凉山彝族末代土司岭光电在彝区推行了一系列社会改革,其主要内容是传播先进文化、争取政治参与、促进社会发展,让彝族民众增强现代国民意识。文化上,岭光电创办边民小学,促进中华文化认同。政治上,岭光电积极争取彝人参政,增进政治认同。社会改良上,岭光电通过破除迷信、提倡医药,奖励植树、发展农业,培养匠人、扶持生产,禁毒戒酒、改革恶习,促进彝区发展进步。岭光电的思想意识与改革实践,既是对历史经验的总结,又是对现实国情的把握。岭光电通过把彝族的发展进步纳入中华民族共同体构建框架的一系列改良实践,促进了彝区民众中华民族共同体意识的觉醒。

关键词：彝族土司国民意识末代土司岭光电凉山彝族

长征时期我党关于民族问题探索的历史演变与现实启示

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民族学刊 2018年第3期9卷 37-42,109,110,111页

作者：马富英依乌西南民族大学彝学学院中国人民解放军军事科学院

中国工农红军长征时期,党高度重视民族问题,制定和实施了一系列符合中国革命和民族实际的民族纲领政策,开创了一条运用马克思主义民族理论解决中国民族问题的探索之路。中国共产党早期的民族纲领与民族政策主张是从马克思列宁主义关于... 详细信息

中国工农红军长征时期,党高度重视民族问题,制定和实施了一系列符合中国革命和民族实际的民族纲领政策,开创了一条运用马克思主义民族理论解决中国民族问题的探索之路。中国共产党早期的民族纲领与民族政策主张是从马克思列宁主义关于民族独立、民族分离、建立联邦或区域自治等方面去阐释。随着我国革命形势的发展变化,党已认识到民族独立或联邦制并不符合中国的国情,不主张民族自决和实行联邦制,并最终确认了在统一的国家中实行民族区域自治的政策。认真分析长征时期党民族理论政策的发展演变进程,对新时期科学总结党民族工作经验和指导民族工作实践,不断发展和完善中国特色社会主义民族理论体系,实现中华民族伟大复兴具有重要意义。

关键词：长征时期民族问题民族政策