检索结果-南通市图书馆

A First-principles Study on the Adhesion Strength,Interfacial Stability,and Electronic Properties of Mg/Mg_(2)Y Interface

引用

Acta Metallurgica Sinica(English Letters) 2024年第3期37卷 537-550页

作者： Yunxuan Zhou Wenjun Tian quan Dong Hailian Wang Jun Tan Xianhua Chen Kaihong Zheng fusheng Pan College of Materials Science and Engineering National Engineering Research Center for Magnesium AlloysChongqing UniversityChongqing 400044China State Key Laboratory of Mechanical Transmission Chongqing UniversityChongqing 400044China Institute of New Materials Guangdong Academy of SciencesGuangzhou 510650China

The interfacial microstructures and configurations directly affect the comprehensive properties of the composites,but their interfacial adhesion mechanism is complicated to expound by experimental *** this work,based on the stacking sequence of the Mg/Mg_(2)Y interface models,nine different Mg/Mg_(2)Y interface configurations with top site,bridge site,and hollow site(HCP)under Mg1,Mg_(2),and Y terminations were successfully constructed and systematically explored by first-principles *** results showed that the Mg_(2)Y(0001)surface with Y termination is the most stable when the yttrium chemical potential()is less than-1.09 eV;otherwise,Mg_(2)Y(0001)surface with Mg1 termination is the most *** seven-layer Mg(0001)and eleven-layer Mg_(2)Y(0001)slabs are employed to reflect the bulk-like interior ***,the Mg(0001)/Mg_(2)Y(0001)with the Y-HCP stacking has the largest interface thermodynamic stability with the value of 2.383 J/m^(2) in all interface configurations owing to its largest work of *** addition,the interfacial energy of Y-HCP stacking is significantly smaller than those of Mg1-HCP when is approximately less than-0.55 eV,showing that it is more *** thermodynamic stability of Mg/Mg_(2)Y with Y-HCP is due to Mg-Y chemical bonds formed between Mg and Y ***,the Mg/Mg_(2)Y interfaces are strong interfaces based on the Griffith fracture theory.

关键词： Mg/Mg_(2)Y interface Interface stability Work of adhesion Electronic structure First-principles calculations

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Recent advancements in thermal conductivity of magnesium alloys

引用

Journal of Magnesium and Alloys 2024年第5期12卷 1687-1708页

作者： Hao Lv Jun Tan Qian Yuan Fanglei Wang Yunxuan Zhou quan Dong Aitao Tang Jürgen Eckert Bin Jiang fusheng Pan National Engineering Research Center for Magnesium Alloys College of Materials Science and Engineering Chongqing University National Key Laboratory of Advanced Casting Technologies Chongqing University Department of Materials Science Montanuniversit?t Leoben Erich Schmid Institute of Materials Science Austrian Academy of Sciences

As highly integrated circuits continue to advance, accompanied by a growing demand for energy efficiency and weight reduction, materials are confronted with mounting challenges pertaining to thermal conductivity and lightweight properties. By virtue of numerous intrinsic mechanisms, as a result, the thermal conductivity and mechanical properties of the Mg alloys are often inversely related, which becomes a bottleneck limiting the application of Mg alloys. Based on several effective modification methods to improve the thermal conductivity of Mg alloys, this paper describes the law of how they affect the mechanical properties, and clearly indicates that peak aging treatment is one of the best ways to simultaneously enhance an alloy's thermal conductivity and mechanical properties. As the most frequently used Mg alloy,cast alloys exhibit substantial potential for achieving high thermal conductivity. Moreover, recent reports indicate that hot deformation can significantly improve the mechanical properties while maintaining, and potentially slightly enhancing, the alloy's thermal conductivity. This presents a meaningful way to develop Mg alloys for applications in the field of small-volume heat dissipation components that require high strength. This comprehensive review begins by outlining standard testing and prediction methods, followed by the theoretical models used to predict thermal conductivity, and then explores the primary influencing factors affecting thermal conductivity. The review summarizes the current development status of Mg alloys, focusing on the quest for alloys that offer both high thermal conductivity and high strength. It concludes by providing insights into forthcoming prospects and challenges within this field.

关键词： Mg alloy Thermal conductivity Mechanical properties Solute atom Second phase

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Modification of alternative splicing in bovine somatic cell nuclear transfer embryos using engineered CRISPR-Cas13d

引用

Science China(Life Sciences) 2022年第11期65卷 2257-2268页

作者： Rui Cheng Xiaoman Zheng Yingmei Wang Xing Ma Xin Liu Wenjun Xu Mengyun Wang Yuanpeng Gao Xupeng Xing Chuan Zhou Hongzheng Sun Zekun Guo fusheng quan Jun Liu Song Hua Yongsheng Wang Yong Zhang Xu Liu College of Veterinary Medicine Northwest A&F UniversityKey Laboratory of Animal Biotechnology of the Ministry of AgricultureYangling 712100China

Animal cloning can be achieved by somatic cell nuclear transfer(SCNT),but the resulting live birth rate is relatively *** previously improved the efficiency of bovine SCNT by exogenous melatonin treatment or by overexpression of lysine-specific demethylase 4D(KDM4D)and 4E(KDM4E).In this study,we revealed abundant alternative splicing(AS)transitions during fertilization and embryonic genome activation,and demonstrated abnormal AS in bovine SCNT embryos compared with in vitro fertilized *** used the CRISPR-Cas13d RNA-targeting system to target cis-elements of ABI2 and ZNF106 pre-m RNA to modify AS,thus reducing the ratio of abnormal-isoform SCNT embryos by nearly 50%and achieving a high survival rate(11%–19%).These results indicate that this system may provide an efficient method for bovine cloning,while also paving the way for further improvements in the efficiency of SCNT.

关键词： animal cloning alternative splicing CRISPR-Cas13d bovine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

miR-101 Regulates Development of Bovine SCNT Embryos via Targeting ING3

miR-101 Regulates Development of Bovine SCNT Embryos via Tar...

引用

中国畜牧兽医学会兽医产科学分会第十三次学术研讨会

作者： Rongxia Xie fusheng quan College of Veterinary Medicine Northwest A&F UniversityKey Laboratory of Animal Biotechnology of the Ministry of Agriculture

The SCNT(somatic cell nuclear transfer) technique is a key technology of modern biotechnology,has been widely used in livestock breeding of excellent individuals,transgenic technology and ***,due to incomplete reprogramming factors,the low birth rate of cloned animals and the high proportion of abnormal individuals affect the further development of SCNT *** evidence suggests that some microRNAs(miRNAs) are involved in the development potential of SCNT *** the abundant miRNA-101 in SCNT donor cells and bovine oocytes,numerous studies have demonstrated that miR-101 can regulate cell proliferation and ***,its roles in bovine SCNT donor cells and embryo development are not *** order to elucidate the roles of miR-101 in bovine SCNT donor cells and embryos,miR-101 was overexpressed in donor cells and the effects on the donor cell proliferation and embryo development were ***,luciferase reporter and western blot assays identified ING3 as a novel target of miR-101 in bovine *** results of Luciferase Reporter demonstrated that the 3’-UTR in ING3 mRNA contained a complementary site for the seed region of *** protein level of ING3 was detected by western blot and the result showed that miR-101 could down-regulate ING3 protein ***,real time PCR and flow cytometry methods were introduced to examine the effects of miR-101 on gene transcripts and cell *** results showed miR-101 reduced ING3 mRNA level,and also inhibited expression of P53,P21,UHRF1,BAX and DNMT3 *** addition,miR-101 promoted the growth of bovine fetal fibroblasts and decreased its ***,the effects of miR-101 overexpression on the development of SCNT embryos and blastocyst quality were *** utilizing donor cells with overexpressed miR-101,the blastocyst rate of bovine SCNT embryos in miR-101 group(35.75%) was significantly higher than the control group(26.06%)(P <0

关键词： miR-101 ING3 SCNT bovine embryo bovine fetal fibroblasts

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

参与调节哺乳动物精子运动的离子通道研究进展

引用

畜牧兽医学报 2023年第7期54卷 2708-2722页

作者：何琪富高峰吴盛辉张涌权富生西北农林科技大学动物医学院杨凌712100 农业部动物生物技术重点实验室杨凌712100 陕西省动物胚胎工程技术研究中心杨凌712100

精子运动能力是精子完成自然受精、实现自然条件下精子-卵母细胞融合的必要条件。精子从雄性附睾经生殖道进入雌性生殖道的过程中,其运动形式可以在很短的时间内根据环境的变化而做出改变,表明精子膜蛋白(离子通道)参与精子运动的调节,... 详细信息

精子运动能力是精子完成自然受精、实现自然条件下精子-卵母细胞融合的必要条件。精子从雄性附睾经生殖道进入雌性生殖道的过程中,其运动形式可以在很短的时间内根据环境的变化而做出改变,表明精子膜蛋白(离子通道)参与精子运动的调节,离子通道的正常表达和受精过程密切相关。目前研究发现Ca^(2+)、K^(+)、Na^(+)等离子通道均参与精子膜极化、运动、获能和顶体反应等生理过程,且通过膜片钳和电生理学等技术初步了解精子离子通道的作用分子机制和拓扑结构。本文就参与调节精子运动的Ca^(2+)、pH、电压门控Na^(+)、K^(+)、Cl^(-)通道的生理功能和拓扑结构进行了总结,期望能为精子离子通道的调节机制和功能研究提供全面的信息,促进动物生殖生物学的发展。

关键词：精子离子通道精子运动 Ca^(2+)/K^(+) 拓扑结构

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

卵泡液细胞外囊泡携带的microRNA对卵泡闭锁影响的研究进展

引用

生物工程学报 2022年第8期38卷 2767-2783页

作者：王莹王晓梅赵蕴琦吴盛辉张涌权富生西北农林科技大学动物医学院农业农村部动物生物技术重点实验室陕西杨凌712100

细胞外囊泡(extracellular vesicles,EVs)是细胞主动释放的膜结合颗粒。在原核生物和真核生物中,EVs被认为是细胞间进行信息交流的一种方式。EVs具有携带蛋白质、脂质和核酸等生物大分子的能力,可以影响亲本细胞和受体细胞的不同生理功... 详细信息

细胞外囊泡(extracellular vesicles,EVs)是细胞主动释放的膜结合颗粒。在原核生物和真核生物中,EVs被认为是细胞间进行信息交流的一种方式。EVs具有携带蛋白质、脂质和核酸等生物大分子的能力,可以影响亲本细胞和受体细胞的不同生理功能。其中,EVs携带的microRNA研究报道最多,在生物体生理功能方面发挥着重要作用。卵泡在发育过程中,只有少数卵泡可以充分发育、成熟、排卵,大多数卵泡在发育的不同阶段发生闭锁。在卵泡发育的整个过程中,每一阶段的变化以及卵泡闭锁调控机制还不完全清楚。本文在总结EVs类型、特性、分离方法及用途的基础上,从不同细胞因子、激素方面重点论述了卵泡液中EVs携带的microRNA是如何调控卵泡闭锁,同时对卵泡液EVs携带的microRNA在生殖调控和生殖疾病诊断方面的研究前景进行了展望,对于卵泡发育调控研究以及有效利用研究具有一定参考意义。

关键词：卵泡液细胞外囊泡 microRNA 卵泡闭锁

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Luteinizing Hormone Induces Androgen Production in Goat Theca Cells via Activation of the PI3K/Akt Pathway

Luteinizing Hormone Induces Androgen Production in Goat Thec...

引用

中国畜牧兽医学会兽医产科学分会第十三次学术研讨会

作者： Pengda Zou Kai Meng Xiaomei Wang Rongxia Xie fusheng quan College of Veterinary Medicine Northwest A&F UniversityKey Laboratory of Animal Biotechnology of the Ministry of Agriculture

Objectives:Theca cells play an important role in maintaining follicles development,particularly,in controlling ovarian steroidogenesis by supplying androgens to granulosa cell for conversion into *** it has been known that steroid hormone production from theca cells is mainly under the control of LH,the intracellular signaling mechanisms that modulate theca cells secrete steroid hormones is *** aim is to have looked at the mechanism of luteinizing hormone(LH) on goat theca cells androgen production,and which is an important step towards better understanding the role of LH in follicle function and development *** and methods:Goat ovaries were collected from 1 to 2 years old goats at a local *** those ovaries without corpus luteum were chosen for the *** cells collected from small antral follicles were treated with LH(1 IU/ml) for various durations(0,4h,6h,8h,12 h,24h,48h).The levels of total-Akt and phospho-Akt were examined using Western *** Findings:Goat theca cells from small antral follicles were incubated with LH for various durations,and phospho-Akt and total-Akt content were examined using Western blotting indicated that the amount of phospho-Akt reached its highest level at 12 h after treatment with ***,theca cells were incubated with LH combined with PI3 K inhibitors for various durations,results show that PI3 K inhibitors inhibited LH-induced Akt phosphorylation in the goat theca ***-PCR analyses showed that LH significantly increased CYP11A1 and CYP17A1 mRNA levels in the goat theca cells(PO.05).Addition of LY294002,significantly decreased LH-induced CYP11A1 and CYP17A1 mRNA expression(P<0.05).However,addition of wortmannin,significantly decreased LH-induced CYP11A1 and CYP17A1 mRNA expression(P<0.05).LH significantly increased LHR mRNA in goat theca cells(P<0.05).Addition of LY294002,but not wortmannin,significantly decreased the expression of LH-induced LHR mRNA(P<0.05

关键词： theca cells LH PI3K/Akt Pathway goat

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Melatonin Stimulates STAR Expression and Progesterone Production Via Activation of The PI3K/AKT Pathway in Bovine Theca Cells

Melatonin Stimulates STAR Expression and Progesterone Produc...

引用

中国畜牧兽医学会兽医产科学分会第十四次学术研讨会

作者： Xiaomei Wang Kai Meng Yuanyuan He Hengqin Wang Yong Zhang fusheng quan College of Veterinary Medicine Northwest A&F University

Melatonin exists in mammal follicular fluid and plays an important role in regulating the steroidogenesis of follicular *** the present study,we studied the effect of melatonin on steroidogenesis

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The Wilms tumor gene(Wt1)(+/-KTS) isoforms regulate steroidogenesis by modulating the PI3k/AKT and MAPK/ERK1/2 pathways in bovine granulosa cells

The Wilms tumor gene(Wt1)(+/-KTS) isoforms regulate steroido...

引用

中国畜牧兽医学会兽医产科学分会第十四次学术研讨会

作者： Kai Meng Xiaomei Wang Yuanyuan He Jiashu Yang Hengqin Wang Yong Zhang fusheng quan College of Veterinary Medicine Northwest A&F University Key Laboratory of Animal Bio-Technology Ministry of AgricultureNorthwest A&F University

The Wilms’tumor gene(Wt1) includes the splicing variants Wt1(+KTS) and Wt1(-KTS).Recent data suggest that Wt1 plays an important role in the development of mice ***,the mechanism through

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

山羊Zfy基因克隆分析及其敲除载体构建

引用

畜牧兽医学报 2022年第3期53卷 711-721页

作者：黄敏谢晓刚何琪富曹旭阳董翔宸康健刘军权富生西北农林科技大学动物医学院杨凌712100 杨凌职业技术学院动物工程分院杨凌712100

旨在对西农萨能奶山羊Zfy基因进行克隆和生物信息学分析,并利用CRISPR/Cas9技术成功获得敲除Zfy基因的奶山羊成纤维细胞株,为进一步探索Zfy基因功能及性别调控提供基础数据。本研究以健康的西农萨能奶山羊为对象,采集3只3月龄公山羊睾... 详细信息

旨在对西农萨能奶山羊Zfy基因进行克隆和生物信息学分析,并利用CRISPR/Cas9技术成功获得敲除Zfy基因的奶山羊成纤维细胞株,为进一步探索Zfy基因功能及性别调控提供基础数据。本研究以健康的西农萨能奶山羊为对象,采集3只3月龄公山羊睾丸组织并提取总RNA。根据NCBI中预测的山羊Zfy基因mRNA序列信息(GenBank登录号:XM_018044893)设计引物,利用RT-PCR技术分段克隆获得山羊Zfy基因序列,使用相关生物信息学软件分析Zfy基因CDS区核苷酸序列并预测其蛋白质的结构和功能。针对山羊Zfy基因CDS区共设计了4条sgRNA,构建4个打靶载体并转染山羊胎儿成纤维细胞(GFFs),利用T7E1酶切检验打靶载体的切割效率,通过嘌呤霉素筛选敲除Zfy基因的GFFs阳性单克隆细胞,经PCR扩增、测序检测突变率。结果显示,成功克隆得到了长度为2406 bp的西农萨能奶山羊Zfy基因CDS区序列。同源性以及系统进化树分析表明,山羊Zfy基因序列与马鹿、牛、绵羊的同源性高,亲缘关系较近,与小鼠的同源性最低,亲缘关系最远。生物信息学软件分析显示,山羊Zfy基因共编码801个氨基酸,分子质量为90.37 ku,Zfy蛋白含66个磷酸化位点,等电点为5.66,亲水性较高,无信号肽,是一个结构不稳定的非分泌蛋白。T7E1酶切发现,4个打靶载体均可发挥敲除活性,切割效率分别为12.31%、40.86%、31.52%、26.37%。选择切割效率最高的打靶质粒转染山羊胎儿成纤维细胞(GFFs),经嘌呤霉素筛选获得能稳定靶向敲除Zfy基因的GFFs阳性细胞株,PCR测序检测突变率为23.91%。综上所述,本研究成功克隆了西农萨能奶山羊Zfy基因并揭示了该基因所编码蛋白的理化性质;成功构建了山羊Zfy基因CRISPR/Cas9敲除载体,并筛选出最佳敲除位点,获得了Zfy基因敲除的亚克隆细胞,为深入研究山羊Zfy基因功能及开发性别控制技术奠定了基础。

关键词： Zfy基因克隆西农萨能奶山羊生物信息学分析基因敲除

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：