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Development of image-based wheat spike counter through a Faster R-CNN algorithm and application for genetic studies

The Crop Journal 2022年第5期10卷 1303-1311页

作者： Lei Li Muhammad Adeel Hassan Shurong Yang furong jing Mengjiao Yang Awais Rasheed Jiankang Wang Xianchun Xia Zhonghu He Yonggui Xiao Institute of Crop Sciences National Wheat Improvement CentreChinese Academy of Agricultural Sciences(CAAS)Beijing 100081China Electronic Information School Foshan PolytechnicFoshan 528137GuangdongChina International Maize and Wheat Improvement Centre(CIMMYT)China Office c/o CAASBeijing 100081China Department of Plant Sciences Quaid-i-Azam UniversityIslamabad 44000Pakistan

Spike number(SN) per unit area is one of the major determinants of grain yield in wheat. Development of high-throughput techniques to count SN from large populations enables rapid and cost-effective selection and facilitates genetic studies. In the present study, we used a deep-learning algorithm, i.e., Faster Region-based Convolutional Neural Networks(Faster R-CNN) on Red-Green-Blue(RGB) images to explore the possibility of image-based detection of SN and its application to identify the loci underlying SN. A doubled haploid population of 101 lines derived from the Yangmai 16/Zhongmai 895 cross was grown at two sites for SN phenotyping and genotyped using the high-density wheat 660 K SNP *** of manual spike number(MSN) in the field, image-based spike number(ISN), and verification of spike number(VSN) by Faster R-CNN revealed significant variation(P < 0.001) among genotypes, with high heritability ranged from 0.71 to 0.96. The coefficients of determination(R^(2)) between ISN and VSN was 0.83, which was higher than that between ISN and MSN(R^(2)= 0.51), and between VSN and MSN(R^(2)= 0.50). Results showed that VSN data can effectively predict wheat spikes with an average accuracy of 86.7% when validated using MSN data. Three QTL ***-4 DS, ***-7 DS, and ***-7 DL were identified based on MSN, ISN and VSN data, while ***-7 DS was detected in all the three data sets. These results indicate that using Faster R-CNN model for image-based identification of SN per unit area is a precise and rapid phenotyping method, which can be used for genetic studies of SN in wheat.

关键词： Deeping learning High-throughput phenotyping QTL mapping RGB imaging

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预制装配式建筑防水技术研究及工程应用

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山西建筑 2018年第29期44卷 103-104页

作者：荆富荣江苏联合职业技术学院南京分院江苏南京210019

介绍了预制装配式建筑防水技术研究重要性,重点分析了预制叠合板保温外挂墙板防水技术、预制空调板防水技术和预制叠合楼板防水技术及其工程应用,旨在提升防水技术应用效率,保证预制装配式建筑工程项目建设质量。

关键词：预制装配式建筑工程防水技术

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应用精英反向学习的引力搜索算法

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计算机应用研究 2015年第12期32卷 3638-3641页

作者：井福荣郭肇禄罗会兰李康顺江西理工大学信息工程学院广州510642 江西理工大学理学院江西赣州341000 华南农业大学信息学院广州510642

引力搜索算法是近年提出的一种颇具潜力的全局优化算法,已经成功应用到了各种工程实践中,然而它在求解复杂工程优化问题时容易出现早熟收敛问题。为了在一定程度上避免早熟收敛现象,提出一种应用精英反向学习策略的引力搜索算法(EOGSA)... 详细信息

引力搜索算法是近年提出的一种颇具潜力的全局优化算法,已经成功应用到了各种工程实践中,然而它在求解复杂工程优化问题时容易出现早熟收敛问题。为了在一定程度上避免早熟收敛现象,提出一种应用精英反向学习策略的引力搜索算法(EOGSA)。在演化进程中,对当前种群中的每个个体分别执行精英反向学习策略,生成一个精英反向种群,并将生成的精英反向种群与当前种群同时进行竞争,选择出下一代种群。在一系列经典函数优化测试问题上的对比实验结果表明,EOGSA算法能够提高传统引力搜索算法的性能,在一定程度上避免早熟收敛的缺点。

关键词：全局优化演化算法精英反向学习引力搜索

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一种应用精英混沌搜索的函数优化算法

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江西理工大学学报 2015年第5期36卷 74-79页

作者：井福荣郭肇禄罗会兰江西理工大学信息工程学院江西赣州341000 江西理工大学理学院江西赣州341000

针对基本FPA算法在求解复杂工程优化问题时存在收敛速度慢的缺点,提出应用精英混沌搜索的花粉授粉算法(CSFPA).CSFPA算法在搜索过程中从当前种群中随机选择出一个个体,对其执行精英混沌搜索操作,从而加快算法的收敛速度.将提出的CSFPA... 详细信息

针对基本FPA算法在求解复杂工程优化问题时存在收敛速度慢的缺点,提出应用精英混沌搜索的花粉授粉算法(CSFPA).CSFPA算法在搜索过程中从当前种群中随机选择出一个个体,对其执行精英混沌搜索操作,从而加快算法的收敛速度.将提出的CSFPA算法与基本FPA算法在几个国际上常用的基准测试问题上进行了比较实验,实验结果表明CSFPA算法能够在大多数测试问题上比基本FPA算法获得更优的结果.

关键词：优化算法演化算法混沌搜索花粉授粉算法

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一种使用反向学习策略的改进花粉授粉算法

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江西理工大学学报 2015年第3期36卷 101-106页

作者：井福荣郭肇禄罗会兰江西理工大学信息工程学院江西赣州341000 江西理工大学理学院江西赣州341000

为了进一步提高基本花粉授粉算法的性能,提出了一种改进的花粉授粉算法(EFPA).该算法在演化过程中以一定的概率利用一般反向学习策略对当前种群作一般反向变换,从而生成一般反向变换种群,然后将一般反向变换种群与当前种群同时进行竞争... 详细信息

为了进一步提高基本花粉授粉算法的性能,提出了一种改进的花粉授粉算法(EFPA).该算法在演化过程中以一定的概率利用一般反向学习策略对当前种群作一般反向变换,从而生成一般反向变换种群,然后将一般反向变换种群与当前种群同时进行竞争,选择出优秀的个体进入下一代种群.在演化计算领域中广泛使用的基准测试函数上,将提出算法与基本花粉授粉算法进行了比较实验,实验结果表明提出算法能够有效地提高基本花粉授粉算法的性能.

关键词：优化算法演化算法反向学习花粉授粉算法

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基于“对分课堂案例讨论”的病理学教学改革探究

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科教导刊 2024年第22期 156-158页

作者：敬芙容古丽沙依拉·队三拜郭雨新疆第二医学院基础医学院病理学教研室新疆克拉玛依834000 新疆第二医学院基础医学院人体解剖学教研室新疆克拉玛依834000

病理学是医学中一门重要的桥梁课。为提高该课程的教学质量,文章围绕2021级3个班临床学生的病理学教学展开探讨,教学中尝试采用“对分课堂+案例讨论”教学模式,并创新性地将讨论环节设置在实验课中,通过课中赋分评价、课后问卷调查、期... 详细信息

病理学是医学中一门重要的桥梁课。为提高该课程的教学质量,文章围绕2021级3个班临床学生的病理学教学展开探讨,教学中尝试采用“对分课堂+案例讨论”教学模式,并创新性地将讨论环节设置在实验课中,通过课中赋分评价、课后问卷调查、期末统考三方面开展教学评价。结果显示,开展新教学模式的班级学生成绩高于传统教学的班级,该教学模式能有效提高学生的教学参与度,提高病理学教学质量。

关键词：病理学对分课堂案例讨论教学方法

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Occurrence Characteristics of Saline-Lacustrine Shale-Oil in the Qianjiang Depression,Jianghan Basin,Central China

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Journal of Earth Science 2022年第4期33卷 945-962页

作者： Zhongliang Sun Zhiliang He furong Wang Yuanjia Han Sheng He Yuguang Hou jing Luo Youheng Zheng Shiqiang Wu Wuxi Research Institute of Petroleum Geology Petroleum Exploration and Production Research InstituteSINOPECWuxi 214126China State Key Laboratory of Shale Oil and Gas Enrichment Mechanisms and Effective Development Beijing 100083China Department of Science and Technology China Petroleum and Chemical CorporationBeijing 100728China Key Laboratory of Tectonics and Petroleum Resources China University of GeosciencesWuhan 430074China Research Institute of Petroleum Exploration and Development Jianghan Oilfield Branch CompanySINOPECWuhan 430223China

The amount of shale oil and its characterization are key issues in the study of shale *** present,many scholars use a variety of methods to evaluate the amount of shale oil,and use the calculated amount of hydrocarbons to analyze its influencing factors;however,there is lack of detailed research on the storage space for shale-oil and it’s influencing *** view of this issue,gas chromatography(GC),gas chromatography-mass spectrometry(GC-MS),soxhlet extraction(SE),fieldemission scanning electron microscopy(FE-SEM),low-temperature nitrogen adsorption(LTNA),highpressure mercury intrusion(HPMI),and X-ray diffraction(XRD)were used to analyze and compare samples from two wells located in different deposition *** unconventional Well BYY2 and BX7 s were drilled in the depocenter and distal area of the Qianjiang Formation,*** by differences in the organic matter type and sedimentary environment,the organic matter in Well BYY2,which was found to be characterized by laminar shale,mainly originated from aquatic *** results showed that as the total organic carbon(TOC)content increased,the amount of shaleoil in the pores ***-oil was mostly stored in mesopores and macropores that had been preserved by dolomite minerals of a biogenic origin and also occurred in pores sized 5–200 nm and>1μ*** minerals of a biogenic origin and clay minerals contributed to the occurrence of *** comparison,the organic matter in Well BX7 has been greatly influenced by terrestrial organic *** in the massive mudstones from Well BX7 were determined to be mainly mesopores preserved by clay minerals and quartz,and the shale-oil was mostly stored within the pores of<40 *** the TOC content was~2.5 wt.%,the generated shale-oil reached *** minerals contributed to the occurrence of shale-oil,whereas quartz only contributed to the occurrence of shale-oil in macropores.

关键词： shale oil reservoir storage space of shale oil saline lacustrine Jianghan Basin

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Synthesis and electromagnetic transport of large-area 2D WTe_(2) thin film

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Journal of Semiconductors 2022年第10期43卷 50-55页

作者： Yumeng Zhang Zhejia Wang Jiaheng Feng Shuaiqiang Ming furong Qu Yang Xia Meng He Zhimin Hu jing Wang Institute of Microelectronics of the Chinese Academy of Sciences Beijing100029China University of Chinese Academy of Sciences Beijing100049China Jiaxing Microelectronics Instrument and Equipment Engineering Center of the Chinese Academy of Sciences Jiaxing 314000China Jiaxing Kemin Electronic Equipment Technology Co. Ltd.Jiaxing 314000China Institute of Physics of the Chinese Academy of Sciences Beijing100190China

Tungsten telluride thin films were successfully prepared on monocrystal sapphire substrates by using atomic layer de-position and chemical vapor deposition technology,and the effects of different tellurization temperatures on the properties of tungsten telluride films were *** growth rate,crystal structure and composition of the film samples were character-ized and analyzed by using scanning electron microscope,Raman spectroscopy and X-ray photoelectron *** res-ults showed that tungsten telluride thin films with good crystal orientation in(001)were obtained at telluride temperature of 550℃.When the telluride temperature reached 570℃,the tungsten telluride began to decompose and unsaturated mag-netoresistance was found.

关键词： atomic layer deposition(ALD) chemical vapor deposition(CVD) telluride temperature WTe_(2)thin film

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Laser projection proximity transfer for deterministic assembly of microchip arrays at scale

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Science China(Technological Sciences) 2022年第9期65卷 2205-2214页

作者： HU JinLong CHEN furong BIAN jing SUN NingNing WANG KaiXin LING Hong YU HaiYang GAI MengXin XU LiZhi HUANG YongAn State Key Laboratory of Digital Manufacturing Equipment and Technology Huazhong University of Science and TechnologyWuhan 430074China Flexible Electronics Research Center Huazhong University of Science and TechnologyWuhan 430074China College of Electronic and Optical Engineering&College of Microelectronics Nanjing University of Posts and TelecommunicationsNanjing 210023China Department of Mechanical Engineering The University of Hong KongHong Kong SAR 999077China

Deterministic assembly techniques that enable programmatic and massively parallel integration of chips are essential for the development of novel electronic systems such as micro LED ***,large-area integration of ultrathin micro-chips with high yield and transfer accuracy remains a great challenge due to the difficulties in selective transfer,adhesion switchability,and transfer ***,a“laser projection proximity transfer(Laser PPT)”technique is presented for the deterministic assembly of microchip arrays at *** of the remarkable features is that the transfer status between the chip and the receiver substrate evolves from the original non-contact mode to contact mode for high-precision transfer,which overcomes the strict requirements of the flatness of stamp and substrate in contact-style transfer,and flight deviation of microchip array in noncontact-style *** feature is the rapid modulation of interfacial adhesion for reliable transfer via the use of thermally expandable microspheres to form microstructures and combining with a laser-induced *** adhesion regulation range is over 20 times without any damage to chip *** results show that the transfer accuracy has been improved substantially with a minimum relative error of~0.5%.Combined with a laser beam projection system,demonstrations of Laser PPT for selective assembly of fragile objects onto challenging non-adhesive/cured surfaces in batch illustrate its potential in the highprecision integration of microscale chips at scale.

关键词： microtransfer printing micro LED display laser lift-off interfacial adhesion

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Single-cell Long Non-coding RNA Landscape of T Cells in Human Cancer Immunity

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Genomics, Proteomics & Bioinformatics 2021年第3期19卷 377-393页

作者： Haitao Luo Dechao Bu Lijuan Shao Yang Li Liang Sun Ce Wang jing Wang Wei Yang Xiaofei Yang Jun Dong Yi Zhao furong Li Translational Medicine Collaborative Innovation Center The Second Clinical Medical College(Shenzhen People’s Hospital)Jinan UniversityShenzhen 518020China Shenzhen Key Laboratory of Stem Cell Research and Clinical Transformation Shenzhen 518020China Integrated Chinese and Western Medicine Postdoctoral Research Station Jinan UniversityGuangzhou 510632China Bioinformatics Research Group Key Laboratory of Intelligent Information ProcessingAdvanced Computing Research CenterInstitute of Computing TechnologyChinese Academy of SciencesBeijing 100190China Department of Gastrointestinal Surgery The Second Clinical Medical College(Shenzhen People’s Hospital)Jinan UniversityShenzhen 518020China

The development of new biomarkers or therapeutic targets for cancer immunotherapies requires deep understanding of *** date,the complete landscape and systematic characterization of long noncoding RNAs(lncRNAs)in T cells in cancer immunity are ***,by systematically analyzing full-length single-cell RNA sequencing(scRNA-seq)data of more than 20,000 libraries of T cells across three cancer types,we provided the first comprehensive catalog and the functional repertoires of lncRNAs in human T ***,we developed a custom pipeline for de novo transcriptome assembly and obtained a novel lncRNA catalog containing 9433 *** increased the number of current human lncRNA catalog by 16%and nearly doubled the number of lncRNAs expressed in T *** found that a portion of expressed genes in single T cells were lncRNAs which had been overlooked by the majority of previous *** on metacell maps constructed by the MetaCell algorithm that partitions scRNA-seq datasets into disjointed and homogenous groups of cells(metacells),154 signature lncRNA genes were *** were associated with effector,exhausted,and regulatory T cell ***,84 of them were functionally annotated based on the co-expression networks,indicating that lncRNAs might broadly participate in the regulation of T cell *** findings provide a new point of view and resource for investigating the mechanisms of T cell regulation in cancer immunity as well as for novel cancer-immune biomarker development and cancer immunotherapies.

关键词： Long non-coding RNA Transcriptome assembly Metacell Immune regulation Functional annotation

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