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Cloning and analysis of expression profile of 13 WRKY genes in rice

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Chinese Science Bulletin 2004年第20期49卷 2159-2168页

作者： QIUYuping JINGShaojuan fujian LILu YUDiqiu KunmingSection XishuangbannaTropicalBotanicalGardenTheChineseAcademyofSciencesKunming650223China GraduateSchooloftheChineseAcademyofSciences Beijing100039China

The transcriptional factor WRKY proteins contain the highly conserved amino acid sequence WRKYGQK as well as the novel zinc-finger-like motifs Cys2His2 or Cys2HisCys. A search of the rice genome identi-fied 97 genes encoding WRKY proteins. Of these 97 WRKY homologs found in rice, 13 cDNAs encoding WRKY proteins were consequently isolated from a rice cDNA library con-structed from 4℃-treated shoots by probing for the con-served WRKY domain. Northern blotting analysis revealed that 10 of 13 OsWRKY genes were differentially regulated in plants that were treated by the four following abiotic stress factors: NaCl,PEG,cold (4℃) and heat (42℃). The resulting expression profiles exhibited great differences in both the manner and timing of their response to the four different abiotic treatments. The difference of gene expression profiles suggested the different physiological functions among the WRKY genes.

关键词：无性繁殖稻基因表达 WRKY基因转录因子非生物应激因子转基因作物

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2022年枣庄市重点职业病监测结果分析

职业与健康 2024年第8期40卷 1029-1033页

作者：郭童褚福见庞英枣庄市疾病预防控制中心职业病防制科山东枣庄277000 枣庄市市中区疾病预防控制中心职业卫生科山东枣庄277100 滕州市疾病预防控制中心职业卫生科山东枣庄277100

目的分析2022年枣庄市接触重点职业病危害因素劳动者的健康状况,为职业卫生监管部门制定相关防治措施提供参考依据。方法依托2022年1月1日—12月31日职业病及健康危害因素监测信息系统上报数据,建立数据库,对重点职业病监测结果异常... 详细信息

目的分析2022年枣庄市接触重点职业病危害因素劳动者的健康状况,为职业卫生监管部门制定相关防治措施提供参考依据。方法依托2022年1月1日—12月31日职业病及健康危害因素监测信息系统上报数据,建立数据库,对重点职业病监测结果异常检出率进行分析。结果在岗劳动者矽尘疑似职业病检出率为0.11%,煤尘疑似职业病检出率为0.04%,噪声疑似职业病检出率为0.003%,矽尘岗前职业禁忌证检出率为0.70%,噪声岗前职业禁忌证检出率为1.07%,苯在岗期间职业禁忌证检出率为0.08%。矽尘作业人员尘肺样改变异常率为0.08%,均为男性,均来自建筑用石加工行业。煤尘作业人员胸片异常主要集中在采矿业,其中2例尘肺样改变均出自烟煤和无烟煤开采洗选业,其他异常783例,异常率11.74%,男性异常率(12.11%)高于女性(4.62%)。接触粉尘劳动者肺功能检查指标异常率从高到低依次为用力肺活量(forced vital capacity,FVC%)异常(17.44%)、1秒用力呼出量(forced expiratory volume in one second,FEV1.0%)异常(5.98%)、FEV1.0/FVC%异常(0.78%)。苯接触工人专项指标其中以白细胞(5.91%)及中性粒细胞减少异常居多(5.58%)。女性白细胞、中性粒细胞、血小板异常率均高于男性。男性双耳高频平均听阈异常率(7.32%)高于女性(1.61%),不同年龄段、工龄段噪声作业工人双耳高频平均听阈异常率有随年龄、工龄增长而升高的趋势。结论 2022年枣庄市职业健康风险最大的职业病危害因素为噪声,其次为粉尘,枣庄市的职业病治理应以尘肺的防治为主,结合职业病危害专项治理及中小微企业职业健康帮扶行动,对超标的石英石板材加工企业进行定点帮扶。

关键词：职业病危害因素职业病职业健康检查

Influence of Intrinsic Decoherence on Nonclassical Effects of System of a Three-LevelAtom in [1] Configuration

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Communications in Theoretical Physics 2004年第2X期42卷 275-280页

作者： XUJing-Bo ZOUXu-Bo GAOXiao-Chun fujian ZhejiangInstituteofModernPhysicsandDepartmentofPhysics ZhejiangUniversityHangzhou310027China

With the help of an SU(3) dynamicalalgebraic structure, we ~nd an exact solutionof the Milburn equationfor the system of a three-levelatom in the [1] configuration interacting with one quantized field mode with arbitrary detuning ,The exact solution is then used to discuss the influence of the intrinsic decoherence on the nonclassical properties of the system,such as collapes and revivals of the atomic populations,oscillations of the photon number distribution,and squeezing of the radiation field.

关键词：非经典效应原子-场相互作用原子物理学三层原子结构本征脱散 Milburn方程

ZnPcS2P2-based Photodynamic Therapy Induces Mitochondria-dependent Apoptosis in K562 Cells

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Acta Biochimica et Biophysica Sinica 2005年第7期37卷 488-494页

作者： Hui-Fang HUANG Yuan-Zhong CHEN Yong WU fujian Institute of Hematology,Union Hospital,fujian Medical university,Fuzhou 350001,China fujian Institute of Hematology Union Hospital Fujian Medical University Fuzhou 350001 China

Mitochondria play a key role in the regulation of apoptosis induced by numerous antitumorchemotherapeutic and other toxic *** therapy （PDT） exerts significant cellular killingefficacy through either an apoptotic or necrotic cell death *** study investigated the mechanismunderlying the killing effects of a novel amphipathic photosensitizer [di-sulfonated di-phthalimidomethylphthalocyanine zinc （ZnPcS2P2）]-mediated photodynamic therapy （ZnPcS2P2-PDT） on K562 *** evident in the post-PDT cells through the TdT-mediated dUTP nick end labeling （TUNEL） method andDNA fragmentation *** ZnPcS2P2-PDT,K562 cells underwent mitochondria-dependent apoptosisas evidenced by the release of cytochrome c from mitochondria into cytosol,accompanied by mitochondrialmembrane potential （ΔΨm） reduction,indicating the opening of the mitochondrial permeability transitionpore （PTP）.The activities of protease from the caspase family and caspase-3 were also significantly ***,ZnPcS2P2-PDT down-regulated the expression of chimaeric Bcr-Abl oncoprotein,which isthe molecular hallmark of chronic myelogenous leukemia （CML）.

关键词： photodynamic therapy zinc phthalocyanine apoptosis mitochondria K562 cell

THE SINGULAR PERTURBATION OF NONLOCAL BOUNDARY VALUE PROBLEMS FOR NONLINEAR ELLIPTIC EQUATION OF FOURTH ORDER

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Systems Science and Mathematical Sciences 2000年第1期13卷 92-96页

作者： CHEN Yu sen (fujian Normal university, Fuqing Branch, Fuqing 350300, China) 福建师范大学福清分校福建福清 350300

Chenandothersstudiedaclassofsingularlyperturbedboundaryproblems[1--3].NowweconsiderthefollowingsingUlarlyperturbednonlocalb***,bistheLaplacian,ndenotesaboundedregi... 详细信息

Chenandothersstudiedaclassofsingularlyperturbedboundaryproblems[1--3].NowweconsiderthefollowingsingUlarlyperturbednonlocalb***,bistheLaplacian,ndenotesaboundedregi...

关键词： Singular perturbation nonlocal boundary value problem asymptotic expansion.

Rheological Behavior of Basalt Fiber Reinforced Asphalt Mastic

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Journal of Wuhan university of Technology(Materials Science) 2014年第5期29卷 950-955页

作者：顾兴宇 XU Tingting NI fujian Transportation College Southeast University Road Maintenance Technology Engineering Center of Jiangsu Province Jiangsu Zhongshe Engineering Consultancy Group Co. LTD

The characters of basalt fiber are analyzed and compared with commonly used fibers. The rheological behaviors of the basalt fiber reinforced asphalt mastic are investigated by the dynamic shear rheological tests and the repeated creep tests. The results show that basalt fiber has excellent reinforced performances, such as high asphalt absorption ratio, low water absorption ratio, high tensile strength, high elastic modulus and high temperature stability. The rutting factor of the fiber reinforced asphalt mastic is higher than the plain asphalt mastic and the reinforced effects are more remarkable under high temperature. The rheological performances of the asphalt mastic demonstrate a good linear relationship between different temperature and loading frequency. The creep stiffness modulus of the asphalt mastic at different loading time can be expressed by power function. Improved Burgers model is used to represent the rheological behaviors of the asphalt mastic with basalt fiber and the model parameters are estimated.

关键词： basalt fiber asphalt mastic dynamic shear rheological test repeated creep test rheological model

Experimental studies of the effects of ZnPcS2P2-based-photodynamic therapy on bone marrow purging

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中华医学杂志(英文版) 2005年第2期000卷 19-24页

作者： HUANG Hui fang, CHEN Yuan zhong and WU Yong fujian Institute of Hematology, Union Hospital, fujian Medical university, Fuzhou 350001, China fujian Institute of Hematology Union Hospital Fujian Medical University Fuzhou 350001 China

Background An effective purging technique plays an important role in autologous hematopoietic stem cells transplantation Photodynamic therapy (PDT) provides a novel approach for this purpose This study dealt with the purging effects of disulfodiphthalimidomethyl phthalolcyanine zinc (ZnPcS2P2)based photodynamic therapy (ZnPcPDT) Methods Fluorescence intensity of cell extracts was measured using a fluoresence spectrophotometry The proliferative potency of K562 cells and HL60 cells was detected using MTT colorimetric assay, Typan blue dye exclusion method, colony formation test The proliferative potency of normal hematopoietic cells was evaluated using mixture colonyforming unit (CFUMix), granulocytemacrophage colonyforming unit (CFUGM), and erythrocyte colonyforming unit (CFUE) assays K562 cells were mixed with normal mononuclear cells (MNCs) at ratios of 1∶100 and 1∶1000 for creating the model of simulated remission bone marrow Colony formation test and nestedRTPCR were carried out to detect the residual K562 cells in cell mixture Results After a 5hour incubation with ZnPcS2P2, the content of ZnPcS2P2 in normal MNCs was the lowest value At the same time, the content in K562 cells and HL60 cells was very high Therefore, the time point was selected as the optimal one for irradiating the cell suspensions ZnPcPDT could significantly kill proliferative K562 cells and HL60 cells in a dosedependent manner At the concentration of 10 μg/ml, the inhibitory rate of ZnPcPDT on the colony formation was 100% for K562 cells, 897% for HL60 cells 025 μg/ml ZnPcPDT could completely photoinactivate residual K562 cells in the simulated remission bone marrow Under an identical condition, the inhibitory rates of CFUMix, CFUGM, CFUE were 180%, 186%, and 178% respectively Conclusion ZnPcPDT appears to be a promising approach for bone marrow purging

关键词： photodynamic therapy · zinc phthalocyanine · bone marrow purging · leukemia

OPTIMAL CONTROL OF SINGULARLY PERTURBED NONLINEAR STATE REGULATOR PROBLEM

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Annals of Differential Equations 1995年第4期11卷 412-419页

作者： Lin Zongchi (fujian Normal university)

In this paper, we consider the optimal control of singularly perturbed nonlinear state regulator by diagonalization techniques. Under the suitable conditions we proved that the given problem has a unique solution (ξ... 详细信息

关键词： singular perturbation. nonlinear state regulator optimal control diagonalization techniques.

A Note on the Multifractal Decomposition of Directed Graph Self Similar Sets

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Wuhan university Journal of Natural Sciences 2004年第3期9卷 269-272页

作者： Zheng Shui-cao 1,2 , Hu Di-he 1 1. School of Mathematics and Statistics, Wuhan university , Wuhan 430072, Hubei, China 2. The Basic Teaching Department, Jimei university, Xiamen 361021, fujian, China Wuhan university School of Mathematics and Statistics Wuhan Hubei China (GRID:grid.49470.3e) (ISNI:***) Jimei university The Basic Teaching Department Xiamen Fujian China (GRID:grid.411902.f) (ISNI:***) Wuhan university School of Mathematics and Statistics Wuhan Hubei China (GRID:grid.49470.3e) (ISNI:***)

We investigated the directed graph self similar sets under some weak overlapping condition. We get the multifractal decomposition formulas for these sets, i. e., dimK u a =DimK u a =f(a), wheref is the multifractal spectral function of the directed graph self similar measure, Especially, the results improve that of Edgar and Mauldin to the case which allows certain overlapping. Key words Hausdorff dimension - Hausdorff measure - multifractal decomposition CLC number O 211. 6 Foundation item: Supported by the National Natural Science Foundation of China (10371092) and the Foundation of Wuhan universityBiography: Zheng Shui-cao (1973-), male, Ph. D candidate, research direction: stochastic processes and random fractals.

关键词： Hausdorff dimension Hausdorff measure multifractal decomposition