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Construction of retroviral vector of p^(125FAK) specific ribozyme genes and its effects on BGC-823 cells

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World Journal of Gastroenterology 2006年第5期12卷 686-690页

作者： Guo-Xian Guan Hong-Xing Jian Dong-Yin Lei Hui-Shan Lu xiang-fu zhang Department of Oncology Affiliated Union Hospital Fujian Medical University Fuzhou 350001 Fujian ProvinceChina

AIM： To construct the retroviral vector of p^125FAK specific ribozyme genes and to explore the feasibility of ribozyme in BGC-823 gene therapy in vitro. METHODS： A hammerhead ribozyme DNA targeting p^125FAK mRNA from nt 1010 to nt 1032 was synthesized and recombinated into the retroviral vector pLXSN forming pLRZXSN recon. Using the lipofectin-mediated DNA transfection technique, pLRZXSN was introduced into BGC-823 cells. The effects of ribozyme on the growth of BGC-823 cells and apoptosis were studied by cell colony assay, flow cytometry （FCM）, reverse transcriptasepolymerase chain reaction （RT-PCR）, detection of DNA fragmentation and electron microscopy. RESULTS： The number of BGC-823 cell colonies was inhibited by 56% after the cells were treated for 48 h. The cell proliferation was inhibited effectively by p^125FAK ribozyme and the inhibitory effect depended on the concentration and the time of incubation. The expression of p^125FAK mRNA and protein p^125 decreased sharply in BGC-823 cells treated with p^125FAK ribozyme. The characteristics of apoptosis, namely sub-G1 peak, DNA fragmentation and morphological changes, were revealed in BGC-823 cells treated with p^125FAK ribozyme. CONCLUSION： p^125FAK ribozyme decreases p^125FAK gene expression and induces apoptosis of human gastric cancer cells in vitro.

关键词： Ribozyme p^12SFAK gene Stomach neoplasm Apoptosis

Effect of lymphadenectomy extent on advanced gastric cancer located in the cardia and fundus

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World Journal of Gastroenterology 2008年第26期14卷 4216-4221页

作者： Chang-Ming Huang Bi-Juan Lin Hui-Shan Lu xiang-fu zhang Ping Li Jian-Wei Xie Department of Oncology Affiliated-Union Hospital of Fujian Medical University Fuzh-ou350001 Fujian Province China

AIM： To analyze the prognostic impact of lymphade-nectomy extent in advanced gastric cancer located in the cardia and fundus. METHODS： Two hundred and thirty-six patients with advanced gastric cancer located in the cardia and fundus who underwent D2 curative resection were analyzed retrospectively. Relationships between the numbers of lymph nodes （LNs） dissected and survival was analyzed among different clinical stage subgroups. RESULTS： The 5-year overall survival rate of the entire cohort was 37.5%. Multivariate prognostic variables were total LNs dissected （P 〈 0.0001; or number of negative LNs examined, P 〈 0.0001）, number of positive LNs （P 〈 0.0001）, T category （P 〈 0.0001） and tumor size （P = 0.015）. The greatest survival differences were observed at cutoff values of 20 LNs resected for stage Ⅱ（p = 0.0136）, 25 for stage Ⅲ（P 〈 0.0001）, 30 for stage Ⅳ（P = 0.0002）, and 15 for all patients （P = 0.0024）. Based on the statistically assumed linearity as best fit, linear regression showed a significant survival enhancement based on increasing negative LNs for patients of stages Ⅲ （P = 0.013） and Ⅳ（P = 0.035）. CONCLUSION： To improve the long-term survival of patients with advanced gastric cancer located in the cardia and fundus, removing at least 20 LNs for stage Ⅱ,25 LNs for stage Ⅲ, and 30 LNs for stage N patients during D2 radical dissection is recommended.

关键词： Stomach neoplasms Lymph node meta-stasis Surgery Lymphadenectomy Prognosis

Prognostic impact of metastatic lymph node ratio in advanced gastric cancer from cardia and fundus

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World Journal of Gastroenterology 2008年第27期14卷 4383-4388页

作者： Chang-Ming Huang Bi-Juan Lin Hui-Shan Lu xiang-fu zhang Ping Li Jian-Wei Xie Department of Oncology Affiliated Union Hospital of Fujian Medical University Fuzhou 350001 Fujian Province China

AIM: To investigate the prognostic impact of the metastatic lymph node ratio (MLR) in advanced gastric cancer from the cardia and fundus. METHODS: Two hundred and thirty-six patients with gastric cancer from the cardia and fundus who underwent D2 curative resection were analyzed ret- rospectively. The correlations between MLR and the total lymph nodes, positive nodes and the total lymph nodes were analyzed respectively. The influence of MLR on the survival time of patients was determined with univariate Kaplan-Meier survival analysis and mul- tivariate Cox proportional hazard model analysis. And the multiple linear regression was used to identify the relation between MLR and the 5-year survival rate of the patients. RESULTS: The MLR did not correlate with the total lymph nodes resected (r = -0.093, P = 0.057). The 5-year overall survival rate of the whole cohort was 37.5%. Kaplan-Meier survival analysis identified that the following eight factors influenced the survival time of the patients postoperatively: gender (χ2 = 4.26, P = 0.0389), tumor size (χ2 = 18.48, P fundus. The decrease of MLR due to adequate number of total resected lymph nodes can improve the survival.

关键词： Lymph node metastasis Metastatic lymph node ratio Lymphadenectomy Prognosis

Preface to special issue: Celebrating the centennial of Chemistry at Nankai University

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Journal of Energy Chemistry 2021年第12期30卷 I0017-I0018页

作者： Fang-Yi Cheng fu-xiang zhang Jun Chen Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) College of ChemistryNankai UniversityTianjin 300071China State Key Laboratory of Catalysis Dalian Institute of Chemical Physics.Chinese Academy of Sciences.Dalian 116023China

Dating back to the founding of Nankai University (NKU) in 1919and initiated by ***-Yue Qiu in 1921,the Department of Chemistry at NKU is one of the earliest chemistry programs in Chinese *** 1923,***-Xian Yang joined ... 详细信息

关键词： founding initiated Nan

Preparation of silver nanoparticles through the reduction of straw-extracted lignin and its antibacterial hydrogel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 504-514页

作者： Lou zhang Shuo Li fu Tang Jingkai zhang Yuetong Kang Hean zhang Lidong Li State Key Laboratory for Advanced Metals and Materials School of Materials Science and EngineeringUniversity of Science and Technology BeijingBeijing 100083China

Silver nanoparticles(Ag NPs)have attracted attention in the field of biomaterials due to their excellent antibacterial ***,the reducing and stabilizing agents used for the chemical reduction of Ag NPs are usually toxic and may cause water *** this work,Ag NPs(31.2 nm in diameter)were prepared using the extract of straw,an agricultural waste,as the reducing and stabilizing *** analysis revealed that the straw extract contained lignin,the structure of which possesses phenolic hydroxyl and methoxy groups that facilitate the reduction of silver salts into Ag *** surfaces of Ag NPs were negatively charged due to the encapsulation of a thin layer of lignin molecules that prevented their *** the prepared Ag NPs were added to the precursor solution of acrylamide,free radical polymerization was triggered without the need for extra heating or light irradiation,resulting in the rapid formation of an Ag NP-polyacrylamide composite *** inhibition zone test proved that the composite hydrogel possessed excellent antibacterial ability due to the presence of Ag *** prepared hydrogel may have potential applications in the fabrication of biomedical materials,such as antibacterial dressings.

关键词： silver nanoparticles hydrogel straw extraction antibacterial

Intensive cholesterol lowering with statin improves the outcomes of percutaneous coronary intervention in patients with acute coronary syndrome

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Chinese Medical Journal 2009年第6期122卷 659-664页

作者： JIA Xin-wei fu xiang-hua zhang Jing GU Xin-shun FAN Wei-ze WU Wei-li HAO Guo-zhen LI Shi-qiang JIANG Yun-fa Department of Cardiovascular Disease Second Hospital of Hebei Medical University Shijiazhuang Hebei 050000 China

Background The incidence of no reflow phenomenon limits the clinical outcomes of percutaneous coronary intervention （PCI）. This randomized controlled study was designed to evaluate the immediate protective effects of intensive statin pretreatment on myocardial perfusion and myocardial ischemic injury during PCI.Methods Altogether 228 patients with acute coronary syndrome （ACS） were randomly assigned to standard statin group （SS group, n=115） and intensive statin group （IS group, n=-113）. Patients in the SS group received 20 mg simvastatin and patients in the IS group received 80 mg simvastatin for 7 days before PCI. Thrombolysis in myocardial infarction （TIMI） flow grade （TFG）, corrected TIMI frame count （CTFC） and TIMI myocardial perfusion grade （TMPG） of the intervened vessel were recorded before and after stent deployment. Creatine kinase （CK） isoenzyme MB, troponin I and plasma level of high sensitive-C reactive protein （hs-CRP）, P-selectin and intercellular adhesion molecule （ICAM） were measured before and 24 hours after the procedure.Results The TFG after stent deployment was significantly improved with less TIMI 0-1 and more TIMI 3 blood flow in the IS group than in the SS group （all P〈0.05）. Patients with no reflow phenomenon were less in the IS group （P〈0.001）. The CTFC was lower in the IS group than in the SS group （P 〈0.001）. TMPG was also improved in the IS group than in the SS group （P=0.001）. Although PCI caused a significant increase in CK-MB 24 hours after the procedure, the elevated CK-MB value was lower in the IS group than in the SS group （18.74±8.41 vs 21.78±10.64, P=0.018）. Similar changes were also found in troponin I （0.99±1.07 in the IS group vs 1.47±1.54 in the SS group, P=0.006）. CK-MB elevation occurred in 27.8% （32/115） of the patients in the SS group vs 15.9% （18/113） in the IS group （P=-0.030）. Myocardial necrosis was detected in 4.4% （5/115） of the patients in the SS group, whereas 0.9% （1/113） in the IS group （P=0.341）. But no myo

关键词： simvastatin myocardium perfusion percutaneous coronary intervention acute coronary syndrome no reflow phenomenon

Quantum public-key crypto via EPR pairs

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 58-63页

作者： xiang-Qun fu Hong-Wei Li Jian-Hong Shi Tan Li Wan-Su Bao Henan Key Laboratory of Quantum Information and Cryptography Information Engineering University Synergetic Innovation Center of Quantum Information and Quantum Physics University of Science and Technology of China

With the development of quantum computing technology, quantum public-key cryptography is gradually becoming an alternative to the existing classical public-key cryptography. This paper designs a quantum trapdoor one-way function via EPR pairs and quantum measurements. Based on this, a new quantum public-key cryptosystem is presented, which offers forward security,and can resist the chosen-plaintext attack and chosen-ciphertext attack. Compared with the existing quantum public-key cryptos,eavesdropping can be automatically detected in this new quantum public-key cryptosystem under a necessary condition, which is also detailed in the paper.

关键词： quantum public-key crypto trapdoor one-way function EPR pairs

边鸡IGF-ⅠR基因AluⅠ位点多态性及其对生长性状和繁殖性状的遗传效应分析

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中国畜牧兽医 2017年第3期44卷 813-818页

作者：吴鹏飞王冬张向前张丽丁馥香张跟喜扬州大学动物科学与技术学院扬州225009 中国农业科学院家禽研究所扬州225125 山西省农业科学院畜牧兽医研究所太原030032

为了寻找与边鸡生长性状和繁殖性状相关的遗传标记,本试验采用PCR-RFLP技术检测边鸡类胰岛素生长因子Ⅰ受体(insulin-like growth factorⅠreceptor,IGF-ⅠR)基因AluⅠ位点的多态性,并分析该多态位点对边鸡生长性状和繁殖性状的遗传效... 详细信息

为了寻找与边鸡生长性状和繁殖性状相关的遗传标记,本试验采用PCR-RFLP技术检测边鸡类胰岛素生长因子Ⅰ受体(insulin-like growth factorⅠreceptor,IGF-ⅠR)基因AluⅠ位点的多态性,并分析该多态位点对边鸡生长性状和繁殖性状的遗传效应。结果显示,边鸡IGF-ⅠR基因AluⅠ位点存在多态性,在外显子2的376bp处有1个G→A突变,产生了GG、GA和AA 3种基因型,基因型频率分别为0.687、0.296和0.017,GG为优势基因型;G等位基因频率为0.835,为优势等位基因。卡方适合性检验结果表明,该基因座位在边鸡群体中处于哈代-温伯格平衡状态(P>0.05)。相关分析结果表明,在8、14、16和18周龄时,GG基因型个体的体重显著高于GA基因型(P<0.05),GG基因型个体的开产体重极显著高于GA基因型个体(P<0.01),但并不能说明该位点可以作为影响边鸡繁殖性状的遗传标记。IGF-ⅠR基因外显子2的AluⅠ位点(G→A)可能是影响边鸡生长性状的一个遗传标记。

关键词：边鸡胰岛素样生长因子-Ⅰ受体基因生长性状繁殖性状 PCR-RFLP

Investigation of bubbles escape behavior from low basicity mold flux for high-Mn high-Al steels using 3D X-ray microscope

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 102-110页

作者： Qiang Liu xiang Li Shen Du Ming Gao Yanbin Yin Jiongming zhang State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China

During the continuous casting process of high-Mn high-Al steels,various types of gases such as Ar need to escape through the top of the *** which,the behavior of bubbles traversing the liquid slag serves as a restrictive link,closely associated with viscosity and the thickness of liquid *** contrast to two-dimensional surface observation,three-dimensional(3D)analysis method can offer a more intuitive,accurate,and comprehensive ***,this study employs a 3D X-ray microscope(3D-XRM)to obtained spatial distribution and 3D morphological characteristics of residual bubbles in mold flux under different basicity of liquid slag,different temperatures,and different holding *** results indicate that as basicity of slag increases from 0.52 to 1.03,temperature increases from 1423 to 1573 K,the viscosity of slag decreases,the floating rate of bubbles *** addition,when holding time increases from 10 to 30 s,the bubbles floating distance increases,and the volume fraction and average equivalent sphere diameter of the bubbles solidified in the mold flux gradually *** one word,increasing the basicity,temperature,and holding time leading to an increase in the removal rate of bubbles especially for the *** findings of bubbles escape behavior provide valuable insights into optimizing low basicity mold flux for high-Mn high-Al steels.

关键词： mold flux low basicity bubbles three-dimensional X-ray microscope viscosity