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CRISPR/Cas9-based Editing of Acetyl-CoA Carboxylase (ACC1) Gene in Barley

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Journal of Northeast Agricultural University(English Edition) 2020年第4期27卷 18-30页

作者： lin Meng-meng Sun Meng Chen Feng-juan Lyu Bo Ni Fei Wu Jia-jie Allan Caplan fu dao-lin State Key Laboratory of Crop Biology Shandong Agricultural UniversityTaian 271018ShandongChina Department of Plant Sciences University of IdahoMoscowID 83844USA Center for Reproductive Biology Washington State UniversityPullmanWA 99164USA

Plastid localized acetyl-CoA carboxylase(ACCase;EC 6.***.1.2)is a target for aryloxyphenoxypropionates(APPs)and cyclohexanediones(CHDs),two groups of selective herbicides used in controlling grassy *** and barley are important cereal crops in the grass(Poaceae or Gramineae)family,and thus sensitive for those *** of this form of ACCase(or ACC1)in wheat and barley is essential if these agents are used in the sustainable *** this study,it was confirmed that a single ACC1 gene presented on the second chromosome per homologous group in common wheat,wild emmer wheat,goat grass and *** CRISPR/Cas9,the barley ACC1 gene was edited,specifically in the carboxyl transferase(CT)domain that was critical for herbicide responses in grass *** new alleles were generated,one with a 3-bp deletion leading to ACC1:***1878del and one with a 26-bp deletion causing ACC1:***2099_*** were recovered as heterozygotes in the T0 *** the seven T0 plants harboring the 3-bp deletion grew normally,but the only T0 plant with 26-bp deletion died at the extension stage(Zadoks 32),probably because there was inadequate ACC1 activity when the plant was *** the T1 generation,the 3-bp deletion(or Ile1878del)did not impact the edited plants in tiller numbers,tiller height,spike length and spikelet numbers,when compared to the wild-type allele in the non-edited *** study demonstrated that CRISPR/Cas9 was practical to generate single amino acid deletions in the ACC1 protein and the Ile1878 deletion did not compromise plant ***,the ACC1:***1878del protein did not confer resistance to the currently tested APP herbicides,including clethodim,haloxyfop,quizalofop-Pethyl and sethoxydim.

关键词： ACCase genome editing herbicide resistance Triticeae wheat

Effects of Long-term Located Fertilization on the Physico-chemical Property of Soil Humus

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Agricultural Sciences in China 2002年第4期1卷 424-431页

作者： SHI Ji-ping, ZHANG fu-dao and lin Bao(School of Agriculture, Shanghai Jiao Tong University, Shanghai 201101 , P.R. China Soil and Fertilizer Inst. CAAS , Beijing 100081 , P.R. China ) School of Agriculture Shanghai Jiao Tong University Shanghai 201101 P.R. China Soil and Fertilizer Inst. CAAS Beijing 100081 P.R. China

A systematic study concerning the effects of a long-term stationary fertilization on content and property of soil humus in fluvo-aquic soil sampled from Malan Farm, Xinji City, Hebei, and arid red soil and paddy red soil sampled from the Institute of Red Soil, Jinxian County, Jiangxi was conducted. The results showed that long-term fertilization had effects not only on the content and composition of soil humus, but also on the physico-chemical property of humus. With applying organic manure or combined application of organic manure and chemical fertilizer, E4 and E6 values of humic acid decreased in fluvo-aquic soil and arid red soil, but increased in paddy red soil. In paddy red soil, E4 and E6 values of humic acid increased also with a single application of chemical fertilizer, but E4 and E6 values had less change of humic acid in fluvo-aquic soil and arid red soil. The effects on the visible spectroscopic property of f ulvic acid were different from that of humic acid. Long-term application of organic manure or combined application of organic manure and chemical fertilizer could increase E4 and E6 values of f ulvic acid in three types of soil. Single application of chemical fertilizer had less effect on the E4 and E6. Long-term fertilization could also influence the ultraviolet spectroscopic property of humus. With a single application of organic manure or combined application of organic manure and chemical fertilizer, the ultraviolet absorbance of humic acid and f ulvic acid increased in the three types of soil. But this effect was obvious only in short wave length, and the effect could decrease if the wave length increased. With a single application of chemical fertilizer the ultraviolet absorbance of fulvic acid could increase, but it of humic acid increased only in fluvo-aquic soil. Long-term application of organic manure or combined application of organic manure and chemical fertilizer could increase the content of total acidic groups, carboxy groups and phe

关键词： Long-term located fertilization Soil humus Fluvo-aquic soil Arid red soil Paddy red soil

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低轮廓高效率的印刷复合空馈阵天线

电波科学学报 2010年第3期25卷 409-414页

作者：吴知航章文勋贺祥符道临东南大学毫米波国家重点实验室江苏南京210096

印刷式复合空馈阵是一种融合印刷反射阵和传输阵概念为一体的新型天线,具有馈电简单、效率高、轮廓低、成本低等特点。研究了两种提高该类天线效率的技术:采用基板和盖板上印刷尺寸渐变的贴片进行相位补偿,使天线口径面的场分布趋于同相... 详细信息

印刷式复合空馈阵是一种融合印刷反射阵和传输阵概念为一体的新型天线,具有馈电简单、效率高、轮廓低、成本低等特点。研究了两种提高该类天线效率的技术:采用基板和盖板上印刷尺寸渐变的贴片进行相位补偿,使天线口径面的场分布趋于同相;并采用"蘑菇型"人工磁导体表面作为基板以抑制横向波,以减小副瓣和交叉极化,由此提高了天线增益。设计工作于14 GHz的天线样机的测试结果与仿真结果吻合,达到近20 dBi的峰值增益,对应82%的口径效率,以及增益跌落少于3dB且回波损失低于-10 dB的8.4%公共频带;天线的剖面总高度仅为12 mm,即0.560λ。

关键词：空馈阵印刷天线低轮廓天线高效率天线宽频带天线人工磁导体

中国研究型大学本科生高影响力教育活动特征探析

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高等教育研究 2020年第2期41卷 58-65页

作者：许丹东吕林海傅道麟南京大学教育研究院江苏南京210093

本研究调查发现,我国研究型大学本科生高影响力教育活动具有八个特征:使学生与他人广泛而深入地交流;为学生创造合作的机会和具有挑战性的任务;知行合一,让学生在实践中学习;有一定的学习自由度,促进学生自主学习;使学生感受到师者风范... 详细信息

本研究调查发现,我国研究型大学本科生高影响力教育活动具有八个特征:使学生与他人广泛而深入地交流;为学生创造合作的机会和具有挑战性的任务;知行合一,让学生在实践中学习;有一定的学习自由度,促进学生自主学习;使学生感受到师者风范;能启发学生的深入思考;提供大量阅读或写作的机会;让学生体验多元文化。中美本科生高影响力教育活动的特征既有共同点也有不同之处。我国研究型大学建设一流本科,要将优质学习发生的共性要素作为高质量学习的核心标准,充分发挥教师对本科生成长的影响作用,加强本科生的阅读和写作训练,将体验多元文化作为本科人才培养的重要组成部分。

关键词：高影响力教育活动大学生学习本科教育多元文化

Bidirectional regulation of the brain-gut-microbiota axis following traumatic brain injury

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Neural Regeneration Research 2025年第8期20卷 2153-2168页

作者： Xinyu You lin Niu Jiafeng fu Shining Ge Jiangwei Shi Yanjun Zhang Pengwei Zhuang National Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional Chinese MedicineTianjinChina Haihe Laboratory of Modern Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjinChina First Teaching Hospital of Tianjin University of Traditional Chinese Medicine TianjinChina National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion TianjinChina

Traumatic brain injury is a prevalent disorder of the central nervous *** addition to primary brain parenchymal damage,the enduring biological consequences of traumatic brain injury pose long-term risks for patients with traumatic brain injury;however,the underlying pathogenesis remains unclear,and effective intervention methods are *** dysfunction is a significant consequence of traumatic brain *** the most densely innervated peripheral tissue in the body,the gut possesses multiple pathways for the establishment of a bidirectional“brain-gut axis”with the central nervous *** gut harbors a vast microbial community,and alterations of the gut niche contribute to the progression of traumatic brain injury and its unfavorable prognosis through neuronal,hormonal,and immune pathways.A comprehensive understanding of microbiota-mediated peripheral neuroimmunomodulation mechanisms is needed to enhance treatment strategies for traumatic brain injury and its associated *** comprehensively reviewed alterations in the gut microecological environment following traumatic brain injury,with a specific focus on the complex biological processes of peripheral nerves,immunity,and microbes triggered by traumatic brain injury,encompassing autonomic dysfunction,neuroendocrine disturbances,peripheral immunosuppression,increased intestinal barrier permeability,compromised responses of sensory nerves to microorganisms,and potential effector nuclei in the central nervous system influenced by gut ***,we reviewed the mechanisms underlying secondary biological injury and the dynamic pathological responses that occur following injury to enhance our current understanding of how peripheral pathways impact the outcome of patients with traumatic brain *** review aimed to propose a conceptual model for future risk assessment of central nervous system-related diseases while elucidating novel insights into the bidirectional effec

关键词： traumatic brain injury brain-gut-microbiome axis gut microbiota neuroimmune immunosuppression host defense vagal afferents bacterial infection dorsal root ganglia nociception neural circuitry

两种改进的Fabry-Perot谐振器印刷天线

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电波科学学报 2008年第2期23卷 300-304页

作者：符道临章文勋顾莹莹葛志晨 YK南大学毫米波国家重点实验室江苏南京210096

对各种Fabry-Perot谐振器印刷天线结构的辐射性能进行了详细比较,从而发展了两种改进结构:采用频率选择表面(FSS)为盖板、U形缝隙矩形贴片为辐射器、以及采用普通电导体或人工磁导体(AMC)为反射板,构成Fabry-Perot谐振器的印刷天线,实... 详细信息

对各种Fabry-Perot谐振器印刷天线结构的辐射性能进行了详细比较,从而发展了两种改进结构:采用频率选择表面(FSS)为盖板、U形缝隙矩形贴片为辐射器、以及采用普通电导体或人工磁导体(AMC)为反射板,构成Fabry-Perot谐振器的印刷天线,实现了高增益、低旁瓣电平、高前后辐射比、较宽的阻抗与增益公共频带等性能,以及低轮廓结构。

关键词： Fabry—Perot谐振器频率选择表面人工磁导体印刷天线

Reducing the measurement errors in nonadiabatic holonomic quantum computers

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 64-70页

作者： Guo-fu Xu Department of Physics Shandong University

Nonadiabatic holonomic quantum computers serve as the physical platform for nonadiabatic holonomic quantum *** quantum computation has entered the noisy intermediate-scale era, building accurate intermediate-scale nonadiabatic holonomic quantum computers is clearly necessary. Given that measurements are the sole means of extracting information, they play an indispensable role in nonadiabatic holonomic quantum computers. Accordingly, developing methods to reduce measurement errors in nonadiabatic holonomic quantum computers is of great importance. However, while much attention has been given to the research on nonadiabatic holonomic gates, the research on reducing measurement errors in nonadiabatic holonomic quantum computers is severely lacking. In this study, we propose a measurement error reduction method tailored for intermediate-scale nonadiabatic holonomic quantum computers. The reason we say this is because our method can not only reduce the measurement errors in the computer but also be useful in mitigating errors originating from nonadiabatic holonomic gates. Given these features,our method significantly advances the construction of accurate intermediate-scale nonadiabatic holonomic quantum computers.

关键词： quantum holonomy quantum computation measurement errors

调控好自己的情绪对大学生的学习有何助益?——基于N大学问卷调查的实证分析

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教学研究 2024年第2期47卷 10-18页

作者：傅道麟吕林海厦门大学教育研究院福建厦门361000 南京大学教育研究院江苏南京210093

相对于“情绪”本身对学习的影响,“情绪调控”所具有的对学生发展的作用越发成为国际学术界关注的前沿主题。在此背景下,“大学生在情绪调控能力方面的人群分布特征”以及“情绪调控与大学生的学习之间的关系”应当成为研究者们探究的... 详细信息

相对于“情绪”本身对学习的影响,“情绪调控”所具有的对学生发展的作用越发成为国际学术界关注的前沿主题。在此背景下,“大学生在情绪调控能力方面的人群分布特征”以及“情绪调控与大学生的学习之间的关系”应当成为研究者们探究的焦点。为此,问卷调查的数据分析获得了如下研究结论:(1)聚类分析和判别分析表明,以情绪调控能力和学习结果作为分类指标的学生群体能够被分为两类,这两类学生群体在学习结果上存在着显著的差异,由此可知,情绪调控能力和学业表现之间存在重要的关联;(2)回归分析的结果显示,情绪调控能力对学生各学习结果变量均具有正向的显著影响。基于上述发现讨论提高教学质量的对策建议。

关键词：大学生学习情绪调控学习满意度深度学习

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麦族WKS基因的重组和遗传转化

山东农业大学学报（自然科学版） 2012年第4期43卷 483-490页

作者：李坤吕波姜辉吴佳洁付道林作物生物学国家重点实验室山东省作物生物学重点实验室山东农业大学山东泰安271018

小麦条锈病是由专性寄生真菌Puccinia striiformis ***引起的重要病害,在世界范围内广泛流行。小麦条锈病抗性基因Wheat Kinase-START 1(WKS1),是近年克隆的高温抗条锈病基因。本研究从二粒小麦、长穗偃麦草、百萨薄冰草和顶芒山羊草中... 详细信息

小麦条锈病是由专性寄生真菌Puccinia striiformis ***引起的重要病害,在世界范围内广泛流行。小麦条锈病抗性基因Wheat Kinase-START 1(WKS1),是近年克隆的高温抗条锈病基因。本研究从二粒小麦、长穗偃麦草、百萨薄冰草和顶芒山羊草中克隆了6个WKS同源基因(WKS1或WKS2)的cDNA片段;通过基因切换创建了WKS1和WKS2之间的重组基因8个;并构建了WKS同源和重组基因相应的植物表达载体。为验证基因功能,分别将WKS同源和重组基因导入了高感锈病的短柄草株系,获得了转基因稳定整合和表达的阳性植株。本项研究为创建新型WKS基因和揭示WKS1基因的作用机理奠定了基础。

关键词：小麦短柄草基因切换条锈病 WKS1