检索结果-南通市图书馆

World Journal of Gastroenterology 2005年第6期11卷 803-809页

作者： Jin-Young jang Sun-Whe Kim ja-Lok Ku Yong-Hyun Park jae-Gahb Park Department of Surgery

AIM: To investigate the effects of gastrin and cholecystokinin (CCK) and their specific antagonists on the growth of pancreatic and biliary tract cancer cell lines. METHODS: Five pancreatic and 6 biliary cancer cell lines with 2 conrtol cells were used in this study. Cell proliferation study was done using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) test and direct cell count method. Reverse transcription-polymerase chain reaction (RT-PCR) and slot blot hybridization were performed to examine and quantify the expression of hormonal receptors in these cell lines. RESULTS: SNU-308 showed a growth stimulating effect by gastrin-17, as did SNU-478 by both gastrin-17 and CCK-8. The trophic effect of these two hormones was completely blocked by specific antagonists (L-365, 260 for gastrin and L-364, 718 for CCK). Other cell lines did not respond to gastrin or CCK. In RT-PCR, the presence of CCK-A receptor and CCK-B/gastrin receptor mRNA was detected in all biliary and pancreatic cancer cell lines. In slot blot hybridization, compared to the cell lines which did not respond to hormones, those that responded to hormones showed high expression of receptor mRNA. CONCLUSION: Gastrin and CCK exert a trophic action on some of the biliary tract cancers.

关键词： Bile duct cancer Gallbladder cancer Pancreatic cancer Gastrin Cholecystokinin

Promoter hypomethylation and reactivation of MAGE-A1 and MAGE-A3 genes in colorectal cancer cell lines and cancer tissues

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第35期12卷 5651-5657页

作者： Kyung-Hee Kim Jin-Sung Choi Il-Jin Kim ja-Lok Ku jae-Gahb Park Laboratory of Cell Biology Korean Cell Line Bank Cancer Research Institute Seoul National University College of Medicine Seoul 110-744 Korea Laboratory of Cell Biology Korean Cell Line Bank Cancer Research Institute Seoul National University College of Medicine Seoul 110-744

AIM： To verify the expression and methylation status of the MAGE-A1 and MAGE-A3 genes in colorectal cancer tissues and cancer cell lines. METHODS： We evaluated promoter demethylation status of the MAGE-A1 and MAGE-A3 genes by RT-PCR analysis and methylation-specific PCR （MS-PCR）, as well as sequencing analysis, after sodium bisulfite modification in 32 colorectal cancer cell lines and 87 cancer tissues. RESULTS： Of the 32 cell lines, MAGE-A1 and MAGE-A3 expressions were observed in 59% and 66%, respectively. Subsequent to sodium bisulfite modification and MSPCR analysis, the promoter hypomethylation of MAGE-A1 and MAGE-A3 was confirmed in both at 81% each. Promoter hypomethylation of MAGE-A1 and MAGE-A3 in colorectal cancer tissues was observed in 43% and 77%, respectively. Hypomethylation of MAGE-A1 and MAGE-A3 genes in corresponding normal tissues were observed in 2% and 6%, respectively. CONCLUSION： The promoter hypomethylation of MAGE genes up-regulates its expression in colorectal carcinomas as well as in gastric cancers and might play a significant role in the development and progression of human colorectal carcinomas.

关键词： MAGE-A1 MAGE-A3 Promoter Hypomethylation Colorectal cancer

Bamboo salt suppresses skin inflammation in mice with 2, 4-dinitrofluorobenzene-induced atopic dermatitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2018年第2期16卷 97-104页

作者： Myoung-schook Yoou Sun-Young Nam Kyoung Wan Yoon Hyun-ja Jeong Hyung-Min Kim Department of Pharmacology College of Korean Medicine Kyung Hee University Department of Biotechnology Hoseo University Department of Food Science & Technology and Research Institute for Basic Science Hoseo University

Bamboo salt(BS) is a traditional Korean food, and has been reported to have anti-cancer, anti-inflammatory, and anti-metastatic effects. However, the anti-atopic dermatitis(AD) activity of BS has not been described yet. In the present study, we examined the preventive effect of BS on AD. The effect of oral administration of BS was tested in a 2, 4-dinitrofluorobenzene(DNFB)-induced AD animal model, by histological analysis, enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, caspase-1 assay, and Western blotting analysis. BS administration reduced the total clinical severity and scratching frequencies, compared with the AD group. In the serum of DNFB-induced AD mice, the levels of IgE, histamine, thymic stromal lymphopoietin(TSLP), interleukin(IL)-5, and IL-13 were significantly reduced by BS treatment. BS significantly reduced the protein and mRNA expression of TSLP, IL-6, and tumor necrosis factor-α in the AD skin lesions. BS markedly reduced the infiltration of inflammatory cells. Furthermore, the activation of caspase-1 was reduced by BS in the AD skin lesions. Our results suggested that BS should be considered as a candidate treatment for allergic inflammatory diseases including AD.

关键词： Bamboo salt Atopic dermatitis Thymic stromal lymphopoietin Caspase-1

Report of a Dicephalic Steppes Ratsnake (Elaphe dione) Collected in South Korea

在线全文

学校读者我要写书评

暂无评论

Asian Herpetological Research 2013年第3期4卷 182-186页

作者： Il-Hun KIM ja-Kyeong KIM Jonathan J.FONG Daesik PARK Department of Biology Kangwon National University School of Biological Sciences Seoul National University Division of Science Education Kangwon National University

In this report, we describe morphological characteristics of a dicephalic Steppes Ratsnake （Elaphe dione） collected from the wild in 2011 in South Korea. The specimen has two heads and two long necks. Unlike normal individuals, the dicephalic snake has divided ventral scales under the necks of the bifurcated columns. The snout- vent length （SVL） and overall total length of the individual are shorter than those of normal snakes of the same age. Nevertheless, the counts of nine different scale types that are often used for classification are all within the ranges of normal individuals. As far as we know, this is the first detailed morphological description of a dicephalic E. dione in the scientific literature.

关键词： dicephalism morphology Steppes Ratsnake Elaphe dione

在线全文

学校读者我要写书评

暂无评论

长沙航电枢纽三叶片转轮改造四叶片转轮浅析

水电站机电技术 2024年第5期47卷 14-18页

作者：尹斌勇纪攀佳李卫黎泽文贺玉平长沙市湘江综合枢纽工程办公室湖南长沙410200

针对长沙航电枢纽水电站机组在枯水期因下游水位下降严重,致使实际水头高于机组最大运行水头而不能运行的问题,以三叶片转轮改造为四叶片转轮技术方案为基础,论述了具体改造方案和相关技术复核工作,经理论分析和实践检验,得出结论:三叶... 详细信息

针对长沙航电枢纽水电站机组在枯水期因下游水位下降严重,致使实际水头高于机组最大运行水头而不能运行的问题,以三叶片转轮改造为四叶片转轮技术方案为基础,论述了具体改造方案和相关技术复核工作,经理论分析和实践检验,得出结论:三叶片转轮改造为四叶片转轮技术方案可行,改造效果良好。机组改造具体方案:①三叶片转轮改造为四叶片转轮,发电机功率因素由0.85调整至0.95,机组容量由9.5 MW增至10.6 MW;②机组改造后,转轮重量增加,水推力增大,进行厂房流道强度复核、振动复核,满足安全运行要求;③同时对机组导水机构、推力轴承、主轴、水导轴承进行改造或更换。供类似航电枢纽的问题处理提供借鉴参考。

关键词：航电枢纽转轮改造

High-performance and current crowding-free InGaN-GaN- based LEDs integrated by an electrically-reverse-connected Schottky diode and a Mg-delta doped p-GaN

在线全文

学校读者我要写书评

暂无评论

Frontiers of Optoelectronics 2012年第2期5卷 127-132页

作者： Sei-Min KIM Seon-Ho jaNG ja-Soon jaNG Department of Electronic Engineering LED-IT Fusion Technology Research Center (LIFTRC) Yeungnam University Gyeongbuk 712 749 Korea

This work demonstrates high-performance and current crowding-free InGaN/GaN light-emitting diodes （LEDs） using an electrically-reverse-connected Schottky diode （SD） and an Mg-delta （δ） doped layer. Possible mechanism of carrier transport at the interface between transparent conducting electrode （TCE） and p-GaN with the δ-doped layer is also investigated. Results show that the LED with the SD and Mg delta （δ）-doping layer yields lower series resistance, higher output power, and lower reverse leakage current compared to normal LEDs. In addition, unlike the normal LED, there is no occurrence for the current crowding effect in the proposed LED even at high current density of 380mA/cm2. These remarkable behaviours clearly indicate that the use of the SD and δ- doping in the p-GaN region is very promising to achieve high-brightness and excellent-reliability GaN-based LEDs.

关键词： GaN light-emitting diode (LED) Schottkydiode (SD) integration current crowding

Effect of pineapple peel extract on total phospholipids and lipid peroxidation in brain tissues of rats

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2011年第3期4卷 182-184页

作者： Erukainure OL Ajiboye ja Adejobi RO Okafor OY Kosoko SB Owolabi FO Food Technology Division Federal Institute of Industrial ResearchOshodiNigeria College of Natural and Applied Sciences Bells University of TechnologyOtaNigeria

Objective:To investigate the ability of the methanolic extract of pineapple peel to attenuate alcohol-induced changes in total phospholipids and lipid peroxidation in brain tissues. Methods:Oxidative stress was induced by oral administration of ethanol(20%w/v) at a dosage of 5 mL/kg bw in *** 28 days of treatment,the rats were fasted overnight and sacrificed by cervical *** tissues were assayed for total phospholipid(TP) content and malondialdehyde(MDA).Results:Administration of alcohol significantly caused a reduction in TP *** with pineapple peel extract significantly increased the TP content. Significant high levels of MDA was observed in alcohol-fed rats,treatment with pineapple peel extract significantly reduced the MDA ***:Results obtained from this study indicates that pineapple peel extract protects against alcohol-induced changes in total phospholipids and lipid peroxidation in brain tissues.

关键词： Brain Malondialdehyde Total phospholipids Alcohol

Male masturbation device for the treatment of delayed ejaculation: A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian pacific Journal of Reproduction 2017年第4期6卷 191-192页

作者： Rodríguez JE Picazo ja López E Research Department of Instituto Sexológico Murciano C/Periodista Encarna Sánchez22030007 MurciaSpain

Delayed ejaculation is a rare condition with different etiologies. Majority of cases of delayed ejaculation have a strong psychological/behavior component. A 48-year-old male with a diagnostic of delayed ejaculation acquired was encouraged to make an exercise protocol using a masturbation device for 8 wk, the percentage of intercourse successful a month after finish protocol exercise was 81%.

关键词： Delayed Ejaculation Male Device Treatment Ejaculation disorders

Post-translational modifications of prostaglandin-endoperoxide synthase 2 in colorectal cancer:An update

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2018年第48期24卷 5454-5461页

作者： Rafael I jaén Patricia Prieto Marta Casado Paloma Martín-Sanz Lisardo Boscá Department of Metabolism and Physiopathology of Inflammatory Diseases Instituto de Investigaciones Biomédicas Alberto Sols (CSIC-UAM) Department of Biomedicine Instituto de Biomedicina de Valencia (CSIC) Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares y Hepáticas y DigestivasISCⅢ Unidad Asociada IIBMULPGC Universidad de las Palmas de Gran Canaria (ULPGC)

The biosynthesis of prostanoids is involved in both physiological and pathological processes. The expression of prostaglandin-endoperoxide synthase 2(PTGS2; also known as COX-2) has been traditionally associated to the onset of several pathologies, from inflammation to cardiovascular, gastrointestinal and oncologic events. For this reason, the search of selective PTGS2 inhibitors has been a focus for therapeutic interventions. In addition to the classic non-steroidal anti-inflammatory drugs, selective and specific PTGS2 inhibitors, termed coxibs, have been generated and widely used. PTGS2 activity is less restrictive in terms of substrate specificity than the homeostatic counterpart PTGS1, and it accounts for the elevated prostanoid synthesis that accompanies several pathologies. The main regulation of PTGS2 occurs at the transcription level. In addition to this, the stability of the mRNA is finely regulated through the interaction with several cytoplasmic elements, ranging from specificmicroR NAs to proteins that control mR NA degradation. Moreover, the protein has been recognized to be the substrate for several post-translational modifications that affect both the enzyme activity and the targeting for degradation via proteasomal and non-proteasomal mechanisms. Among these modifications, phosphorylation, glycosylation and covalent modifications by reactive lipidic intermediates and by free radicals associated to the proinflammatory condition appear to be the main changes. Identification of these post-translational modifications is relevant to better understand the role of PTGS2 in several pathologies and to establish a correct analysis of the potential function of this protein in diseases progress. Finally, these modifications can be used as biomarkers to establish correlations with other parameters, including the immunomodulation dependent on molecular pathological epidemiology determinants, which may provide a better frame for potential therapeutic interventions.

关键词： Prostaglandins Prostaglandin-endoperoxide synthase 2 Post-translational modifications Glycosylation Colorectal cancer Inflammation

通心络胶囊对糖尿病大鼠心功能和心肌重塑的影响

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

宁夏医学杂志 2024年第3期46卷 185-189页

作者：张雄慧贾丽张常喜闫文瑞杨珂珂宁夏中西医结合医院宁夏银川750021 宁夏回族自治区体检康复保健中心宁夏银川750002 宁夏回族自治区中医医院宁夏银川750021 宁夏医科大学宁夏银川750004

目的研究通心络胶囊对糖尿病大鼠心功能及心肌重塑的影响。方法选取Spargue Dawley(SD)雄性大鼠83只,用2%链脲佐菌素(STZ)30 mg/kg造模后,将其随机分为通心络高、中、低剂量组、缬沙坦组、糖尿病组。各组灌胃10周;监测其体重及血糖;大... 详细信息

目的研究通心络胶囊对糖尿病大鼠心功能及心肌重塑的影响。方法选取Spargue Dawley(SD)雄性大鼠83只,用2%链脲佐菌素(STZ)30 mg/kg造模后,将其随机分为通心络高、中、低剂量组、缬沙坦组、糖尿病组。各组灌胃10周;监测其体重及血糖;大鼠麻醉后测定其心功能,测定心脏质量指数,ELISA法测定大鼠心肌匀浆TGF-β1、Caspase-3、bcl-2蛋白含量。结果通心络胶囊的高、中、低剂量组及缬沙坦组较糖尿病组精神状态更活跃,毛色有光泽,多食、多饮、多尿症状减轻,大便较干燥,体重增加。造模后,通心络高、中、低剂量组、缬沙坦组及糖尿病组较空白对照组明显下降,灌胃4~10周,各组较空白对照组差异均有统计学意义(P0.05)。心室间隔厚度、室后壁厚度、室舒张末期内径、左室收缩末期内径、左室舒张末期容积、左室收缩末期容积指标比较,缬沙坦组和通心络高剂量组较糖尿病组各指标均明显降低(P0.05)。bcl-2指标中,缬沙坦和通心络高、中剂量组较糖尿病组明显增加(P<0.05),通心络高剂量组明显高于缬沙坦组(P<0.05)。结论通心络胶囊可以明显改善糖尿病大鼠的心功能、抑制心肌重塑,疗效可观。

关键词：糖尿病心肌病 SD大鼠心功能心肌重塑