检索结果-南通市图书馆

山楂叶总黄酮通过miR-21a-5p对血管紧张素Ⅱ诱导的心肌细胞肥大的影响

在线全文

学校读者我要写书评

暂无评论

中国临床药理学杂志 2024年第15期40卷 2182-2186页

作者：田林燕朱冉代聚平王佳佳张斌南阳医学高等专科学校内科教研室河南南阳473000 南阳医学高等专科学校临床实训室河南南阳473000 南阳医学高等专科学校第一附属医院心血管内科河南南阳473000 南阳医学高等专科学校第一附属医院病理科河南南阳473000

目的探讨山楂叶总黄酮对血管紧张素Ⅱ(AngⅡ)诱导的心肌肥大的影响,及其可能作用机制。方法将H9c2细胞分为对照组(正常培养),模型组(100 nmol·L^(-1)AngⅡ处理24 h),低、中、高剂量实验组(先给予100 nmol·L^(-1)AngⅡ处理24 h后,再分... 详细信息

目的探讨山楂叶总黄酮对血管紧张素Ⅱ(AngⅡ)诱导的心肌肥大的影响,及其可能作用机制。方法将H9c2细胞分为对照组(正常培养),模型组(100 nmol·L^(-1)AngⅡ处理24 h),低、中、高剂量实验组(先给予100 nmol·L^(-1)AngⅡ处理24 h后,再分别给予25、50、100 mg·L^(-1)的山楂叶总黄酮处理24 h),anti-miR-NC、anti-miR-21a-5p组(分别转染anti-miR-NC、anti-miR-21a-5p后,给予100 nmol·L^(-1)AngⅡ处理24 h),miR-NC+高剂量组和miR-21a-5p+高剂量组(分别转染miR-NC、miR-21a-5p mimics,再给予100 nmol·L^(-1)AngⅡ+100 mg·L^(-1)山楂叶总黄酮各处理24 h)。用CCK-8法和流式细胞术检测细胞活力和凋亡情况,用实时荧光定量聚合酶链反应法检测miR-21a-5p的表达水平,用蛋白质印迹法检测环氧化酶-2(COX2)/前列腺素E2(PGE2)的表达水平。结果对照组、模型组和高剂量实验组的细胞活力分别为1.03±0.09、0.51±0.05和0.93±0.08,凋亡率分别为(7.69±0.61)%、(23.04±1.82)%和(9.43±0.71)%,miR-21a-5p表达水平分别为1.00±0.09、2.43±0.18和1.09±0.08,COX2蛋白相对表达水平分别为0.42±0.03、0.85±0.08和0.40±0.04,PGE2蛋白相对表达水平分别为0.34±0.03、0.75±0.07和0.35±0.03,模型组的上述指标与高剂量实验组和对照组相比,在统计学上差异均有统计学意义(均P<0.05)。anti-miR-NC组、anti-miR-21a-5p组、miR-NC+高剂量组和miR-21a-5p+高剂量组的细胞活力分别为0.52±0.04、1.12±0.08、0.94±0.09和0.57±0.04,凋亡率分别为(23.04±1.82)%、(9.86±0.73)%、(9.47±0.64)%和(24.96±1.94)%,miR-21a-5p表达水平分别为1.00±0.10、0.43±0.04、1.00±0.09和2.12±0.18,COX2蛋白相对表达水平分别为0.86±0.05、0.39±0.04、0.41±0.03、0.78±0.07,PGE2蛋白相对表达水平分别为0.74±0.06、0.38±0.07、0.36±0.02、0.71±0.05,anti-miR-21a-5p组的上述指标与anti-miR-NC组比较,miR-21a-5p+高剂量组的上述指标与miR-NC+高剂量组比较,在统计学上差异均有统计学意义(均P<0.05)。结论山楂叶总黄酮通过调控miR-21a-5p和COX2/PGE2通路的表达,从而抑制AngⅡ诱导的心肌肥大。

关键词：山楂叶总黄酮心肌肥大血管紧张素Ⅱ 细胞增殖凋亡

Preparation of Diamond—Like carbon Films in methane by Electron Cyclotron Resonance Microwave Plasma Source Ion Implantation

在线全文

学校读者我要写书评

暂无评论

Chinese Physics Letters 2003年第5期20卷 692-695页

作者： LIXin TANGZhen-An MAGuo-ja WUZhi-Meng DengXin-Lu DepartmentofElectronicEngineering MicrosystemResearchCenterDalianUniversityofTechnologyDalian116024 StateKeyLaboratoryofMaterialsModificationbyLaser IonandElectronBeamsDalianUniversityofTechnologyDalian116024

Diamond-like carbon(DLC)films were prepared on Si(100) substrates by ion implantation from an electron cyclotron resonance microwave plasma *** the implantation,650W microwave power was used to produce discharge plasma with methane as working gas,and-20KV voltage pulses were applied to the substrate holder to accelerate ions in the *** Raman spectra confirmed the DLC characteristics of the ***-transform infrared characterization indicates that the DLC films were composed of sp^3 and sp^2 carbonbonded *** hardness of the films was evaluated with a Nano Indenter-XP *** result shows that the highest hardness valus was 14.6 *** surface rms roughness of the films was as low as 0.104nm measured with an atomic force *** friction coeffcient of the films was checked using a ball-on-disk *** average friction coefficient is approximately 0.122.

关键词：硅衬底等离子体源电子回旋加速器薄膜生长离子注入微波加速器拉曼光谱傅里叶变换

Insulinotrophic and hypolipidemic effects of Ecklonia cava in streptozotocin-induced diabetic mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2012年第5期5卷 374-379页

作者： Mi-ja Kim Hye Kyung Kim Department of Food and Biotechnology Hanseo UniversitySeosan 356-706Republic of Korea Department of Pharmacology school of MedicineKyunghee UniversitySeoul 130-701Republic of Korea

Objective:To explore the anti-diabetic activity of Ecklonia cava(EC) in streptozotocin(STZ)-induced diabetic ***:Diabetes was induced by a single intraperitoneal injection of STZ(90 mg/kg).Normal and diabetic mice were treated with 0%,3%,and 5%EC diet for 4 weeks. Serum glucose and insulin concentrations,serum lipid profile,oral glucose tolerance test,and liver and pancreaticβ-cell histopathological observations were *** addition,in vitro glucose-induced insulin secretion was determined using pancreaticβ-islet ***:EC supplementation significantly and dose-dependently decreased serum glucose concentration, and improved glucose homeostasis in diabetic mice by preventing loss ofβ-cell mass resulting in increase of insulin *** triglyceride and total cholesterol concentrations in the serum and liver were markedly reduced by EC treatment in STZ-diabetic ***,LDL-,and HDL-cholesterol levels were ameliorated in EC supplemented diabetic *** steatosis induced by STZ was ameliorated by EC ***,in vitro insulinotrophic effect of EC extract was observed in pancreaticβ-***:This study demonstrated that EC is a potent and efficacious hypoglycemic and hypolipidemic agent,and prevents the loss ofβ-cell mass resulting in increase of insulin secretary capacity.

关键词： Diabetes Streptozotocin Insulin Lipid profile Pancreaticβ-cell

Helicobacter pylori infection in gastric mucosa-associated lymphoid tissue lymphoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2014年第11期20卷 2751-2759页

作者： Jeong Bae Park ja Seol KooJeong Department of Internal Medicine College of MedicineDongguk UniversityGyeongju 780-350South Korea Division of Gastroenterology and Hepatology Department of Internal MedicineKorea University Ansan HospitalAnsan 425-707South Korea

Gastrointestinal lymphoma is the most common type of extranodal lymphoma,and most commonly affects the *** zone B-cell lymphoma of the mucosa-associated lymphoid tissue(MALT)and diffuse large B-cell lymphoma are the most common histologic types of gastric *** its increasing incidence,diagnosis of gastric lymphoma is difficult at an earlier stage due to its nonspecific symptoms and endoscopic findings,and,thus,a high index of suspicion,and multiple,deep,repeated biopsies at abnormally and normally appearing sites in the stomach are *** addition,testing for Helicobacter pylori(***)infection and endoscopic ultrasonography to determine the depth of tumor invasion and involvement of regional lymph nodes is essential for predicting response to *** eradication and for assessment of disease *** addition,*** infection and MALT lymphoma development are associated,and complete regression of low-grade MALT lymphomas after *** eradication has been *** and/or chemotherapy can be used in cases that show poor response to *** eradication,negativity for *** infection,or high-grade lymphoma.

关键词： Mucosa-associated lymphoid tissue Lymphoma Helicobacter pylori Eradication Remission

CONJUGATE FLOWS OVER A STEP IN A THREE-LAYER FLUID

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Hydrodynamics 2002年第4期14卷 15-23页

作者： Wei, Gang Le, ja-Chun Dai, Shi-Qiang Inst. of Appl. Math. and Mech. Shanghai Univ. Shanghai 200072 China Sch. of Sci. P.L.A Univ. of Sci. and Eng. Nanjing 211101 China

The conjugate flows over a step with the height h_0, which might be positive or negative, was studied in a three-layer fluid and the coupled nonlinear equations were derived, with which the effects of varying height h_0 on the existence and evolution of conjugate flows were examined. It is concluded that the conjugate flow is sharply sensitive to the thickness of fluid layers and its characteristics alters remarkably due to the existence of the step. As the flow climbs up a step (h_0>0), the conjugate flow with a convex lower interface and a concave upper interface is allowed to appear, while the flow with the concave lower interface or the simultaneous concave interfaces will be depressed. As the flow goes down a step (i.e., h_0<0), on most occasions only one kind of conjugate flow could exist, which prossesses the form with the simultaneous convex interfaces and will disappear rapidly with the increase of the step depth.

关键词： conjugate flow flat solitary wave stratified flow step topography three-layer fluid

Crowdsourcing geographic information for disaster response:a research frontier

在线全文

学校读者我要写书评

暂无评论

International Journal of Digital Earth 2010年第3期3卷 231-241页

作者： Michael F.Goodchild ja.lan Glennon Center for Spatial Studies University of CaliforniaSanta BarbaraCA 93106-4060USA

Geographic data and tools are essential in all aspects of emergency management:preparedness,response,recovery,and *** information created by amateur citizens,often known as volunteered geographic information,has recently provided an interesting alternative to traditional authoritative information from mapping agencies and corporations,and several recent papers have provided the beginnings of a literature on the more fundamental issues raised by this new *** quality is a major concern,since volunteered information is asserted and carries none of the assurances that lead to trust in officially created *** emergencies time is the essence,and the risks associated with volunteered information are often outweighed by the benefits of its *** example is discussed using the four wildfires that impacted the Santa Barbara area in 20072009,and lessons are drawn.

关键词： emergency management volunteered geographic information crowdsourcing Web 2.0 neogeography wildfire Santa Barbara

Nonlinear Local Lyapunov Exponent and Quantification of Local Predictability

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Physics Letters 2008年第5期25卷 1919-1922页

作者：丁瑞强李建平 HA Kyung-ja State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics (LASG) Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 Division of Earth Environmental ~System Pusan National University Busan 609- 735 Korea

Nonlinear local Lyapunov exponent （NLLE） is applied to quantitatively determine the local predictability limit of chaotic systems. As an example, we find that the local predictability limit of Henon attractor varies considerably with time, and some underlying phase-spatial structure does not appear. The local predictability limit of initially adjacent points in phase space may be completely different. This will cause difficulties in making the long-time analogue forecast.

关键词： ATMOSPHERIC PREDICTABILITY

EFFECT OF LOADING TYPE ON FATIGUE CRACK GROWTH BEHAVIOR OF SOLDER JOINT IN ELECTRONIC PACKAGING

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Mechanica Solida Sinica 2014年第3期27卷 245-258页

作者： Xishu Wang Huaihui Ren Bisheng Wu Su ja Norio Kawagoishi Department of Engineering Mechanics AML Tsinghua University China Longyuan Power Group Corporation Limited China Goudian CSIRO Earth Science and Resource Engineering Clayton VIC 3168 Australia Department of Mechanical System Engineering Daiichi Institute of TechnologyKagoshima 899-4395 Japan

Fatigue cracking tests of a solder joint were carried out using in-situ scanning electron microscopy （SEM） technology under tensile and bending cyclic loadings. The method for predicting the fatigue life is provided based on the fatigue crack growth rate of the solder joint. The results show that the effect of the loading type on the fatigue crack growth behavior of a solder joint cannot be ignored. In addition, the finite element analysis results help quantitatively estimate the response relationship between solder joint structures. The fatigue crack initiation life of a solder joint is in good agreement with the fatigue life （N50%） of a totally electronic board with 36 solder joints.

关键词： microcracking fatigue MEMS experimental techniques finite element method

探究早期帕金森病Thy 1-SNCA模型小鼠运动学习障碍的时间特征

在线全文

学校读者我要写书评

暂无评论

首都医科大学学报 2024年第5期45卷 881-890页

作者：付文欣王铁杨汝宁曲明芹高歌杨慧贾军首都医科大学基础医学院生理学与病理生理学学系北京100069 首都医科大学基础医学院神经生物学学系北京100069

目的本实验通过建立早期帕金森病(Parkinson s disease,PD)小鼠模型,以探究PD模型出现运动学习障碍的时间特征。方法选用6月龄Thy 1-SNC A转基因(transgenic,TG)小鼠作为早期PD模型,与同龄野生型(wild type,WT)小鼠进行对比分析。通过... 详细信息

目的本实验通过建立早期帕金森病(Parkinson s disease,PD)小鼠模型,以探究PD模型出现运动学习障碍的时间特征。方法选用6月龄Thy 1-SNC A转基因(transgenic,TG)小鼠作为早期PD模型,与同龄野生型(wild type,WT)小鼠进行对比分析。通过旷场实验、爬杆实验和Y迷宫等行为学手段来评估小鼠的运动功能和空间工作记忆能力,并以经典的匀加速转棒实验作为运动学习的行为学范式,来评估其运动学习能力。结果6月龄的Thy 1-SNC A TG小鼠在运动功能和空间记忆能力方面并未显示出异常,却呈现出明显的运动学习障碍。结论在早期PD模型小鼠上存在着特定的运动学习障碍,可作为早期PD的一个重要诊断指标;同时匀加速转棒测试可作为检测PD运动学习障碍的一种重要的行为学手段。

关键词：帕金森病早期帕金森病模型运动学习障碍运动症状