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Anticancer property of sediment actinomycetes against mCF-7 and mDA-mB-231 cell lines

Asian Pacific Journal of Tropical Biomedicine 2012年第2期2卷 92-96页

作者： Ravikumar S fredimoses m Gnanadesigan m School of marine Sciences Department of Oceanography and Coastal Area StudiesAlagappa UniversityThondi CampusThondi-623 409Ramanathapuram District Department of Biotechnology K.S.Rangasamy College of Arts & ScienceThiruchengodu- 637209Namakkal District

Objective:To investigate the anticancer property of marine sediment actinomvceles against two different breast cancer cell ***:In vitro anticancer activity was carried out against breast(mCF-7 and mDA-mB-231)cancer cell *** sequences of the 16s rRNA gene,phylogenetic tree construction,multiple sequence analysis and secondary structure analysis were also carried out with the actinomycetes ***:Of the selected five actinomycete isolates,ACT01 and ACT02 showed the IC_(50)value with(10.13±0.92)and(22.34±5.82)μg/mL concentrations,respectively for mCF-7 cell line at 48 h,but ACT01 showed the minimum(18.54±2.49μg/mL)level of IC_(50)value with mDA-mB-231 cell ***,the 16s rRNA partial sequences of ACT01,ACT02,ACT03,ACT04 and ACT05 isolates were also deposited in NCBI data bank with the accession numbers of GQ478246,GQ478247,GQ478248,GQ478249 and *** phylogenetic tree analysis showed that,the isolates of ACT02 and ACT03 were represented in groupⅠandⅢ,respectively,but ACT01 and ACT02 were represented in groupⅡ.The multiple sequence alignment of the actinomycete isolates showed that,the maximum identical conserved regions were identified with the nucleotide regions of 125 to 221st base pairs,65 to 119th base pairs and 55,48 and 31st base *** structure prediction of the 16s rRNA showed that,the maximum free energy was consumed with ACT03 isolate(-45.4 kkal/mol)and the minimum free energy was consumed with ACT04 isolate(-57.6 kkal/mol).Conclusions:The actinomycete isolates of ACT01 and ACT02(GQ478246 and GQ478247)which are isolated from sediment sample can be further used as anticancer agents against breast cancer cell lines.

关键词： Actinomyetes Breast cancer mCF-7 mDA-mB-231 Phylogenetic tree Anticancer property multiple sequence analysis Secondary structure analysis Sediment Anticaner agent Cell line

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